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1. 发明授权

US06287771B1 Method for determining mastitis using keratin and casein which are amplified by primers 失效
标题翻译：使用通过引物扩增的角蛋白和酪蛋白来确定乳腺炎的方法
公开(公告)号：US06287771B1
公开(公告)日：2001-09-11
申请号：US09297960
申请日：1999-05-27
申请人： Mio Imamura , Yasuharu Itagaki , Morimasa Tanimoto
发明人： Mio Imamura , Yasuharu Itagaki , Morimasa Tanimoto
IPC分类号： C12Q168
CPC分类号： C07K14/4732 , C07K14/4741 , C12Q1/6883 , C12Q2600/158
摘要： A novel primer is provided which is useful in the reverse transcription polymerase chain reaction to amplify &agr;S1-casein gene and keratin gene contained in milk. A method is also provided for using the expression ratio of their genes to examine the pathological conditions of mammalian mastitis or determine the milk protein synthesis level in mammary gland cells. The method is quick and accurate and avoids the need for excising mammary gland tissue.
摘要翻译：提供了一种新的引物，其可用于逆转录聚合酶链反应以扩增牛奶中所含的αS1-酪蛋白基因和角蛋白基因。还提供了使用其基因的表达比率来检查哺乳动物乳腺炎的病理状况或确定乳腺细胞中的乳蛋白质合成水平的方法。该方法快速准确，避免了切除乳腺组织的需要。

2. 发明授权

US6143560A Method of synchronizing epithelial cells into G.sub.0 phase 失效
标题翻译：将上皮细胞同步到G0期的方法
公开(公告)号：US6143560A
公开(公告)日：2000-11-07
申请号：US179525
申请日：1998-10-27
申请人： Mio Imamura , Yasuharu Itagaki , Morimasa Tanimoto
发明人： Mio Imamura , Yasuharu Itagaki , Morimasa Tanimoto
IPC分类号： C12N15/09 , C12N5/071 , C12N5/10 , C12N5/00
CPC分类号： C12N5/0631 , C12N2503/00 , C12N2517/10
摘要： The present invention relates to a method of synchronizing epithelial cells into G.sub.0 phase, more specifically, a method of synchronizing epithelial cells into G.sub.0 phase characterized by culturing the cells in a synthetic medium for 5 days or more, wherein said synthetic medium is a medium with the lowered concentration of bovine fetal serum 5% or less and said epithelial cells is bovine mammary gland cells. Further, the present invention relates to a method of culturing cells characterized by using G.sub.0 phase synchronized cells obtained by the present invention, and a method of screening a related to differentiation and/or maturation of said cells by using G.sub.0 phase synchronized cells. The present invention is useful for easily synchronizing epithelial cells into G.sub.0 phase and for screening a factor related to differentiation and/or maturation of epithelial cells.
摘要翻译：本发明涉及将上皮细胞同步至G0期的方法，更具体地说，涉及将上皮细胞同步至G0期的方法，其特征在于在合成培养基中培养细胞5天以上，其中所述合成培养基为牛胎儿血清浓度降低5％以上，所述上皮细胞为牛乳腺细胞。此外，本发明涉及一种培养细胞的方法，其特征在于使用通过本发明获得的G0相同步细胞，以及通过使用G0相同步细胞筛选与所述细胞的分化和/或成熟相关的方法。本发明可用于容易地将上皮细胞同步化为G0期并筛选与上皮细胞的分化和/或成熟有关的因子。

3. 发明授权

US5866146A Compositions for fair-skin 失效
标题翻译：公平皮肤的组成
公开(公告)号：US5866146A
公开(公告)日：1999-02-02
申请号：US804821
申请日：1997-02-24
申请人： Yasuharu Itagaki , Morimasa Tanimoto , Seiji Kurosawa , Tetsuro Ohba , Klaas Doesburg , Jan Sikkema , Taisuke Iwasaki
发明人： Yasuharu Itagaki , Morimasa Tanimoto , Seiji Kurosawa , Tetsuro Ohba , Klaas Doesburg , Jan Sikkema , Taisuke Iwasaki
IPC分类号： C12P19/04 , A61K8/00 , A61K8/73 , A61K8/96 , A61K8/99 , A61Q19/02 , C08B37/00 , C12R1/225 , C12R1/48 , A61K7/00 , A61K7/48 , A61K31/715
CPC分类号： A61Q19/02 , A61K8/73 , A61K8/99
摘要： This invention provides compositions for fair-skin containing the phosphorylated polysaccharides produced by lactic acid bacteria as an effective ingredient. The phosphorylated polysaccharides are expressed, for example, by the following formula and contained in the compositions for fair-skin at concentrations of 0.01-20 weight % and are used such as creams, emulsions and toilet lotions.The compositions for fair-skin exhibit fair-skin effect by inhibiting activities to melanoma proliferation and melanin formation. ##STR1## (wherein, Glc, Gal and Rha represent glucose, galactose and rhamnose residues, respectively. Numerals shown by other than subscript represent the respective binding sites and n represents the degree of polymerization of 1,000-5,000).
摘要翻译：本发明提供了含有由乳酸菌生产的磷酸化多糖作为有效成分的合适皮肤的组合物。磷酸化的多糖例如通过下式表达，并且含量在0.01-20重量％浓度的公平皮肤组合物中，并且使用例如乳膏，乳液和洗手液。通过抑制黑色素瘤增殖和黑色素形成的活性，公平皮肤的组合物显示出公平的皮肤效果。（I）（其中，Glc，Gal和Rha分别代表葡萄糖，半乳糖和鼠李糖残基，除下标所示的数字表示各自的结合位点，n表示聚合度为1,000-5,000）。

4. 发明授权

US5112754A Tissue plasminogen-activating factor and nonoclonal antibody 失效
标题翻译：组织致细胞因子激活因子和非分子抗体
公开(公告)号：US5112754A
公开(公告)日：1992-05-12
申请号：US483800
申请日：1990-02-23
申请人： Akira Suzuki , Yasuharu Itagaki , Kanji Higashio
发明人： Akira Suzuki , Yasuharu Itagaki , Kanji Higashio
IPC分类号： G01N33/53 , A61K38/00 , A61K39/395 , C07K1/22 , C07K14/005 , C07K14/195 , C07K14/745 , C07K16/00 , C07K16/40 , C12N15/02 , C12P21/08 , C12R1/91 , G01N33/577
CPC分类号： C07K14/745 , C07K16/40 , A61K38/00
摘要： A novel tissue plasminogen activator having the following characteristics: molecualr weight of 65,000-72,000 Daltons as measured by SDS-PAGE electrophoresis using at 7.5% agarose gel; plasminogen activator specific activity of about 10.4.times.10.sup.4 IU/mg, wherein specific activity is defined as the ratio of fibrinolytic activity of purified t-PA measured on fibrin-agarose plates to milligrams of protein; about 83.1% absorption of t-PA by a fibrin-Sepharose column when applied; binds to a Concanavalin A column when applied; the fibrinolytic activity is substantially undiminished by heating at 60.degree. C. for 60 minutes or 95.degree. C. for 5 minutes relative to unheated t-PA; unreactive with polyclonal antisera raised against urokinase; the fibrinolytic activity is substantially stable at pH 5-10; exhibits fibrinolytic activity at pH 7.5-9.0 and temperature 39.degree.-41.degree. C.; a Km value of about 1.16.times.10.sup.-3 mol/liter and a V.sub.max of about 11.7.times.10.sup.-8 mol/liter for substrate S-2288; and fibrinolytic activity is inhibited by Co.sup.2+ Zn.sup.2+, Cd.sup.2+, Hg.sup.2+, Ni.sup.2+ and Cu.sup.2+.
摘要翻译：具有以下特征的新型组织纤溶酶原激活物：通过使用7.5％琼脂糖凝胶的SDS-PAGE电泳测量的分子量为65,000-72,000道尔顿; 约10×10 14 IU / mg的纤溶酶原激活剂比活度，其中比活性定义为在纤维蛋白 - 琼脂糖平板上测量的纯化t-PA的纤维蛋白溶解活性与毫克蛋白质的比例; 应用时纤维蛋白 - 琼脂糖凝胶柱吸收t-PA约83.1％; 应用时可结合伴刀豆球蛋白A柱; 相对于未加热的t-PA，通过在60℃加热60分钟或95℃5分钟，纤维蛋白溶解活性基本上没有减弱; 与尿激酶相关的多克隆抗血清无反应; 纤维蛋白溶解活性在pH 5-10时基本稳定; 在pH 7.5-9.0和39°-41℃下呈现纤维蛋白溶解活性。对于基材S-2288，Km值为约1.16×10-3摩尔/升，Vmax为约11.7×10-8摩尔/升; Co2 ++ L，Zn2 +，Cd2 +，Hg2 +，Ni2 +和Cu2 +可抑制纤维蛋白溶解活性。

5. 发明授权

US5587359A Human derived glycoprotein, biologically active factor which includes glycoprotein and pharmaceutical product 失效
标题翻译：人源性糖蛋白，包括糖蛋白和药物产品的生物活性因子
公开(公告)号：US5587359A
公开(公告)日：1996-12-24
申请号：US304419
申请日：1994-09-12
申请人： Kanji Higashio , Shinjiro Mitsuda , Nobuyuki Shima , Yasuharu Itagaki , Masaya Nagao
发明人： Kanji Higashio , Shinjiro Mitsuda , Nobuyuki Shima , Yasuharu Itagaki , Masaya Nagao
IPC分类号： C12N15/00 , A61K38/00 , C07K14/475 , C07K14/52 , C12N15/09 , C12N15/19 , C12P21/02 , C07H17/00 , A01N37/00
CPC分类号： C07K14/4753 , C07K14/475 , A61K38/00
摘要： The new glycoprotein, which possesses an anti-tumor activity, a leukemia cell differentiation inducing activity, a cellular immunology enhancing activity, a vascular endothelial cell growth stimulating activity and a hepatocyte growth stimulating activity, can be obtained.
摘要翻译：可以获得具有抗肿瘤活性，白血病细胞分化诱导活性，细胞免疫增强活性，血管内皮细胞生长刺激活性和肝细胞生长刺激活性的新糖蛋白。

6. 发明授权

US06333309B1 Human-derived glycoprotein, biologically active factor which includes glycoprotein, and pharmaceutical product which comprises biologically active factor as active component 失效
标题翻译：人源性糖蛋白，包括糖蛋白的生物活性因子和包含生物活性因子作为活性成分的药物产品
公开(公告)号：US06333309B1
公开(公告)日：2001-12-25
申请号：US08617621
申请日：1996-03-19
申请人： Kanji Higashio , Shinjiro Mitsuda , Nobuyuki Shima , Yasuharu Itagaki , Masaya Nagao
发明人： Kanji Higashio , Shinjiro Mitsuda , Nobuyuki Shima , Yasuharu Itagaki , Masaya Nagao
IPC分类号： A61K746
CPC分类号： C07K14/4753 , A61K38/00 , C07K14/475
摘要： The new glycoprotein, which possesses an anti-tumor activity, a leukemia cell differentiation inducing activity, a cellular immunology enhancing activity, a vascular endothelial cell growth stimulating activity and a hepatocyte growth stimulating activity, can be obtained.
摘要翻译：可以获得具有抗肿瘤活性，白血病细胞分化诱导活性，细胞免疫增强活性，血管内皮细胞生长刺激活性和肝细胞生长刺激活性的新糖蛋白。

7. 发明授权

US5698185A Whitening cosmetic preparation and method of using same 失效
标题翻译：美白化妆品及其使用方法
公开(公告)号：US5698185A
公开(公告)日：1997-12-16
申请号：US231190
申请日：1994-04-22
申请人： Yasuharu Itagaki , Hidetoshi Ishikawa , Seiji Kurosawa
发明人： Yasuharu Itagaki , Hidetoshi Ishikawa , Seiji Kurosawa
IPC分类号： A61K8/72 , A61K8/00 , A61K8/64 , A61K8/96 , A61K8/98 , A61Q19/00 , A61Q19/02 , A61Q19/10 , A61K7/48
CPC分类号： A61K8/64 , A61K8/986 , A61Q19/02 , A61Q19/10
摘要： A whitening cosmetic preparation containing angiogenin as an effective component is disclosed. Angiogenin used in the present invention may be derived from milk, especially bovine milk. The whitening cosmetic preparation contains at least 0.001% by weight, preferably from 0.01 to 20% by weight angiogenin based on the total amount of said preparation. A method for whitening skin comprising administration of angiogenin to skin is also disclosed. The whitening cosmetic preparation inhibits melanin production and prevents the developments of blotches and freckles of skin.
摘要翻译：公开了含有血管生成素作为有效成分的美白化妆品。用于本发明的血管生成素可以来自牛奶，特别是牛奶。基于所述制剂的总量，美白化妆品包含至少0.001重量％，优选0.01至20重量％的血管生成素。还公开了一种使皮肤美白的方法，包括给皮肤施用血管生成素。美白化妆品抑制黑色素生成并防止皮肤斑点和雀斑的发展。

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