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1. 发明授权

US06365341B1 Stabilization of highly sensitive nucleic acid stains in aqueous solutions 有权
标题翻译：在水溶液中稳定高灵敏度的核酸污渍
公开(公告)号：US06365341B1
公开(公告)日：2002-04-02
申请号：US09535129
申请日：2000-03-24
申请人： Minjie Wu , Hugh W. White , Noriko Kusukawa , Thomas M. Stein
发明人： Minjie Wu , Hugh W. White , Noriko Kusukawa , Thomas M. Stein
IPC分类号： C12Q100
CPC分类号： G01N33/52 , C07H19/00
摘要： The present invention discloses the use of quaternary compounds as stabilizing agents for highly-sensitive fluorescent nucleic acid stains in aqueous solvents, their use in gels to give increased usable shelf life, and in compositions of solvents, providing ready-to-use stain solutions.
摘要翻译：本发明公开了季铵化合物作为水性溶剂中高灵敏度荧光核酸污渍的稳定剂的用途，它们在凝胶中的用途以提高可用的保质期，以及在溶剂组合物中提供即用型污渍溶液。

2. 发明授权

US06635427B2 Single-labeled oligonucleotide probes for homogeneous nucleic acid sequence analysis 有权
公开(公告)号：US06635427B2
公开(公告)日：2003-10-21
申请号：US09927842
申请日：2001-08-10
申请人： Carl T. Wittwer , Andrew O. Crockett , Brian E. Caplin , Wade Stevenson , Jian Chen , Noriko Kusukawa
发明人： Carl T. Wittwer , Andrew O. Crockett , Brian E. Caplin , Wade Stevenson , Jian Chen , Noriko Kusukawa
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , C12Q1/6818 , C12Q1/6851 , C12Q2565/107 , C12Q2525/185
摘要： Probes and methods are provided for detection and analysis of nucleic acid sequences. The probes are single-labeled oligonucleotide probes whose fluorescence emission changes in response to probe-target hybridization and dissociation. The methods are for analyzing one or multiple nucleic acid loci using the probes. This invention further relates to the use of fluorescence changes in single-labeled probes for melting curve analysis, genotyping, and pathogen detection, and to methods for quantification of specific sequences in real-time monitoring of nucleic acid amplification.

3. 发明授权

US06464850B1 Method for producing hydrophilic monomers and uses thereof 有权
标题翻译：亲水性单体的制造方法及其用途
公开(公告)号：US06464850B1
公开(公告)日：2002-10-15
申请号：US09363167
申请日：1999-07-29
申请人： Tianhong Zhang , Noriko Kusukawa , Mark Garner
发明人： Tianhong Zhang , Noriko Kusukawa , Mark Garner
IPC分类号： G02N2726
CPC分类号： G01N27/44747
摘要： The invention relates to a method for producing hydrophilic monomers which are particularly useful for electrophoresis and to electrophoresis compositions and coating compositions. The electrophoresis gel compositions and electrophoresis polymer compositions are hydrolytically stable and have high resolution. The method uses the steps of reacting a (meth)acryloyl with an aminoalcohol in the presence of a base in a polar solvent, optionally filtering an aqueous solution of the reaction product, deionizing an aqueous solution of the reaction product, and removing the solvent.
摘要翻译：本发明涉及亲水性单体的制造方法，其特别适用于电泳和电泳组合物和涂料组合物。电泳凝胶组合物和电泳聚合物组合物具有水解稳定性和高分辨率。该方法使用以下步骤：在碱存在下使极性溶剂中的（甲基）丙烯酰基与氨基醇反应，任选地过滤反应产物的水溶液，使反应产物的水溶液去离子，并除去溶剂。

4. 发明授权

US6117293A Method for producing hydrophilic monomers and uses thereof 失效
标题翻译：亲水性单体的制造方法及其用途
公开(公告)号：US6117293A
公开(公告)日：2000-09-12
申请号：US127770
申请日：1998-07-31
申请人： Tianhong Zhang , Noriko Kusukawa
发明人： Tianhong Zhang , Noriko Kusukawa
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44747
摘要： The invention relates to a method for producing hydrophilic monomers which are particularly useful for electrophoresis and to electrophoresis compositions and coating compositions. The method uses the steps of reacting a (meth)acryloyl with an aminoalcohol in the presence of a base in a polar solvent, optionally filtering an aqueous solution of the reaction product, deionizing an aqueous solution of the reaction product, and removing the solvent.
摘要翻译：本发明涉及亲水性单体的制造方法，其特别适用于电泳和电泳组合物和涂料组合物。该方法使用以下步骤：在碱存在下使极性溶剂中的（甲基）丙烯酰基与氨基醇反应，任选地过滤反应产物的水溶液，使反应产物的水溶液去离子，并除去溶剂。

5. 发明授权

US5641626A Highly derivatized agarose conformational nucleic acid separation 失效
标题翻译：高度衍生化的琼脂糖构象核酸分离
公开(公告)号：US5641626A
公开(公告)日：1997-06-24
申请号：US146286
申请日：1993-10-29
申请人： Maxine Dumais , Noriko Kusukawa , Hugh White
发明人： Maxine Dumais , Noriko Kusukawa , Hugh White
IPC分类号： C08L5/12 , C12Q1/68 , G01N27/447 , H01L29/788
CPC分类号： C12Q1/6827 , C08L5/12 , G01N27/44747 , Y10T436/143333
摘要： Compositions for the gel electrophoresis separation of conformational isomers of nucleic acids and agarose compositions useful in such compositions. The compositions use compositions comprising: at least one aqueous gel which is at least one highly derivatized agarose derivatized sufficiently to reduce its gelling temperature (Tg) to below 17.degree. C., present in 40 wt % or more based upon the gel total weight, in admixture with at least one high gel-strength polysaccharide aqueous gel other than polyacrylamide in a balance to 100% total gel weight; and an electrophoretic buffer, present in an electrophoretic buffer--effective amount. The invention compositions comprise at least one aqueous gel comprising at least one agarose derivatized sufficiently to reduce its gelling temperature (Tg) to below 9.degree. C., present in 40 wt % or more based upon the gel total weight, in admixture with at least one high strength non-acrylamide polysaccharide present in a balance to 100% total gel weight; and an electrophoretic buffer, present in an electrophoretic buffer--effective amount.
摘要翻译：用于凝胶电泳分离用于此类组合物的核酸和琼脂糖组合物的构象异构体的组合物。组合物使用组合物，其包含：至少一种水凝胶，其至少一种高度衍生化的琼脂糖，其足以将其胶凝温度（Tg）降低至低于17℃，基于凝胶总重量以40重量％或更多存在，与除了聚丙烯酰胺之外的至少一种高凝胶强度多糖水性凝胶混合，其余至100％总凝胶重量; 和电泳缓冲液存在于电泳缓冲液有效量中。本发明组合物包含至少一种包含至少一种琼脂糖的水性凝胶，所述琼脂糖充分地衍生化，以将凝胶化温度（Tg）降低至低于9℃，基于凝胶总重量以40重量％或更多的比例混合，至少与一种高强度非丙烯酰胺多糖存在于100％总凝胶重量的平衡中; 和以电泳缓冲液有效量存在的电泳缓冲液。

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