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1. 发明授权

US5641626A Highly derivatized agarose conformational nucleic acid separation 失效
标题翻译：高度衍生化的琼脂糖构象核酸分离
公开(公告)号：US5641626A
公开(公告)日：1997-06-24
申请号：US146286
申请日：1993-10-29
申请人： Maxine Dumais , Noriko Kusukawa , Hugh White
发明人： Maxine Dumais , Noriko Kusukawa , Hugh White
IPC分类号： C08L5/12 , C12Q1/68 , G01N27/447 , H01L29/788
CPC分类号： C12Q1/6827 , C08L5/12 , G01N27/44747 , Y10T436/143333
摘要： Compositions for the gel electrophoresis separation of conformational isomers of nucleic acids and agarose compositions useful in such compositions. The compositions use compositions comprising: at least one aqueous gel which is at least one highly derivatized agarose derivatized sufficiently to reduce its gelling temperature (Tg) to below 17.degree. C., present in 40 wt % or more based upon the gel total weight, in admixture with at least one high gel-strength polysaccharide aqueous gel other than polyacrylamide in a balance to 100% total gel weight; and an electrophoretic buffer, present in an electrophoretic buffer--effective amount. The invention compositions comprise at least one aqueous gel comprising at least one agarose derivatized sufficiently to reduce its gelling temperature (Tg) to below 9.degree. C., present in 40 wt % or more based upon the gel total weight, in admixture with at least one high strength non-acrylamide polysaccharide present in a balance to 100% total gel weight; and an electrophoretic buffer, present in an electrophoretic buffer--effective amount.
摘要翻译：用于凝胶电泳分离用于此类组合物的核酸和琼脂糖组合物的构象异构体的组合物。组合物使用组合物，其包含：至少一种水凝胶，其至少一种高度衍生化的琼脂糖，其足以将其胶凝温度（Tg）降低至低于17℃，基于凝胶总重量以40重量％或更多存在，与除了聚丙烯酰胺之外的至少一种高凝胶强度多糖水性凝胶混合，其余至100％总凝胶重量; 和电泳缓冲液存在于电泳缓冲液有效量中。本发明组合物包含至少一种包含至少一种琼脂糖的水性凝胶，所述琼脂糖充分地衍生化，以将凝胶化温度（Tg）降低至低于9℃，基于凝胶总重量以40重量％或更多的比例混合，至少与一种高强度非丙烯酰胺多糖存在于100％总凝胶重量的平衡中; 和以电泳缓冲液有效量存在的电泳缓冲液。

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