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1. 发明授权

US5792668A Radio frequency spectral analysis for in-vitro or in-vivo environments 失效
标题翻译：射频频谱分析体外或体内环境
公开(公告)号：US5792668A
公开(公告)日：1998-08-11
申请号：US631916
申请日：1996-04-15
申请人： Milton E. Fuller , David W. Deamer , Mark N. Iverson , Ajit J. Koshy, deceased
发明人： Milton E. Fuller , David W. Deamer , Mark N. Iverson , Ajit J. Koshy, deceased
IPC分类号： A61B5/00 , A61B5/05 , A61B5/053 , G01N22/00 , G01N33/487 , G01N27/00 , G01N33/50
CPC分类号： A61B5/14532 , A61B5/053 , Y10T436/144444 , Y10T436/171538
摘要： Concentration of a target chemical in the presence of other substances in a specimen is determined by subjecting the specimen to radio frequency electromagnetic components, sequentially or otherwise, ranging to about 5 GHz. The reflected and/or transmitted signal real and imaginary components at the specimen are spectrally examined as a function of frequency to identify the presence and/or concentration of the chemical of interest. Such examination includes analysis of the effective complex impedance presented by the specimen, and/or effective phase shift between the transmitted and reflected signal at the specimen. The effects upon glucose concentration measurements of varying electrolytes, primarily NaCl, can be nulled-out by examining impedance magnitude at a cross-over frequency, for example about 2.5 GHz. NaCl concentration exhibits a very linear relationship with phase shift change at frequencies in the 2 GHz-3 GHz range. In a specimen that is blood, such phase shift measurements provide data proportional to NaCl concentration. Impedance magnitude measurements using 1 MHz to 400 MHz frequencies provides a measure of combined concentration of glucose and NaCl. The phase shift data may then be used to substrate out the NaCl concentration from the combined concentration, to yield a good measure of glucose concentration. Such tests may be conducted in-vitro or in-vivo and lend themselves to blood level glucose analyses by diabetics.
摘要翻译：在样本中存在其他物质的情况下，目标化学物质的浓度是通过对样本进行射频电磁成分的测定，依次或其他方式，范围为约5 GHz。在样品处的反射和/或传输的信号实部和虚部被频谱地检查作为频率的函数，以识别感兴趣的化学物质的存在和/或浓度。这种检查包括对样品所呈现的有效复阻抗的分析和/或样品的透射和反射信号之间的有效相移。通过检查交叉频率（例如约2.5 GHz）处的阻抗幅度，可以对不同电解质（主要是NaCl）对葡萄糖浓度测量的影响无效。 NaCl浓度与2 GHz -3 GHz频段的相移变化呈非常线性关系。在血液样本中，这种相移测量提供与NaCl浓度成比例的数据。使用1 MHz至400MHz频率的阻抗幅度测量提供了葡萄糖和NaCl的组合浓度的量度。然后可以将相移数据用于从组合浓度中提取NaCl浓度，以产生良好的葡萄糖浓度测量值。这样的测试可以在体外或体内进行，并且可以借助糖尿病患者进行血液葡萄糖分析。

2. 发明授权

US07189503B2 Characterization of individual polymer molecules based on monomer-interface interactions 有权
公开(公告)号：US07189503B2
公开(公告)日：2007-03-13
申请号：US10739585
申请日：2003-12-18
申请人： Mark Akeson , Daniel Branton , George Church , David W. Deamer
发明人： Mark Akeson , Daniel Branton , George Church , David W. Deamer
IPC分类号： G01N33/483
CPC分类号： G01N33/48721 , B82Y15/00 , B82Y30/00 , C07K14/705 , C12N9/1241 , C12Q1/6816 , C12Q1/6827 , C12Q1/6869 , G01N33/68 , Y10S977/795 , Y10S977/88 , Y10S977/953 , Y10S977/957 , Y10S977/958 , C12Q2565/631 , C12Q2523/307 , C12Q2565/607
摘要： The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.

3. 发明授权

US4515736A Method for encapsulating materials into liposomes 失效
标题翻译：将材料包封在脂质体中的方法
公开(公告)号：US4515736A
公开(公告)日：1985-05-07
申请号：US493952
申请日：1983-05-12
申请人： David W. Deamer
发明人： David W. Deamer
IPC分类号： A61K9/127 , A61K9/52 , B01J13/02
CPC分类号： A61K9/1278 , A61K9/1277 , Y10S424/812 , Y10S436/829 , Y10T428/2984
摘要： A novel encapsulation method is provided in which liposome dispersions are dried in the presence of a material to be encapsulated. As drying occurs, the individual liposomes fuse to form multilamellar structures which capture the material between lipid lamellae. Upon rehydration, lipid vesicles form which efficiently encapsulate the material.
摘要翻译：提供了一种新颖的包封方法，其中在待包被的材料存在下将脂质体分散体干燥。当干燥发生时，各个脂质体融合形成多层结构，其捕获脂质薄片之间的材料。在再水合时，形成有效地包封材料的脂质囊泡。

4. 发明授权

US08500982B2 Compositions, devices, systems, and methods for using a nanopore 有权
标题翻译：使用纳米孔的组合物，器件，系统和方法
公开(公告)号：US08500982B2
公开(公告)日：2013-08-06
申请号：US12450633
申请日：2008-04-04
申请人： Mark A. Akeson , David W. Deamer , William B. Dunbar , Noah A. Wilson , Kathy Lieberman
发明人： Mark A. Akeson , David W. Deamer , William B. Dunbar , Noah A. Wilson , Kathy Lieberman
IPC分类号： G01N27/453 , G01N27/327
CPC分类号： C12Q1/6874 , C12Q1/54 , C12Q1/6869 , C25B3/10 , G01N27/3278 , G01N27/4166 , G01N33/48721 , C12Q2522/101 , C12Q2565/631
摘要： The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. The devices and methods are also used to determine rapidly (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
摘要翻译：本文公开的本发明提供了可以检测和控制混合物中的单个聚合物在纳米孔中被另一种化合物（例如酶）作用的装置和方法。所述装置和方法还用于在反馈控制下或使用通过多核苷酸和纳米孔之间的相互作用产生的信号来快速（〜> 50Hz）确定多核苷酸的核苷酸碱基序列。本发明特别用于分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域。

5. 发明申请

US20110174625A1 COMPOSITIONS, DEVICES, SYSTEMS, AND METHODS FOR USING A NANOPORE 有权
标题翻译：组合物，设备，系统和使用纳米级的方法
公开(公告)号：US20110174625A1
公开(公告)日：2011-07-21
申请号：US12450633
申请日：2008-04-04
申请人： Mark A. Akeson , David W. Deamer , Roger Jinteh Arrigo Chen , Seico Benner , Daniel Branton , William B. Dunbar , Noah A. Wilson , Kate Lieberman , Robin Abu-Shumays , Nicholas Hurt
发明人： Mark A. Akeson , David W. Deamer , Roger Jinteh Arrigo Chen , Seico Benner , Daniel Branton , William B. Dunbar , Noah A. Wilson , Kate Lieberman , Robin Abu-Shumays , Nicholas Hurt
IPC分类号： G01N27/447
CPC分类号： C12Q1/6874 , C12Q1/54 , C12Q1/6869 , C25B3/10 , G01N27/3278 , G01N27/4166 , G01N33/48721 , C12Q2522/101 , C12Q2565/631
摘要： The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. The devices and methods are also used to determine rapidly (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
摘要翻译：本文公开的本发明提供了可以检测和控制混合物中的单个聚合物在纳米孔中被另一种化合物（例如酶）作用的装置和方法。所述装置和方法还用于在反馈控制下或使用通过多核苷酸和纳米孔之间的相互作用产生的信号来快速（〜> 50Hz）确定多核苷酸的核苷酸碱基序列。本发明特别用于分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域。

6. 发明申请

US20110005918A1 Compositions, devices, systems, and methods for using a nanopore 审中-公开
标题翻译：使用纳米孔的组合物，器件，系统和方法
公开(公告)号：US20110005918A1
公开(公告)日：2011-01-13
申请号：US12080684
申请日：2008-04-04
申请人： Mark A. Akeson , David W. Deamer , Roger Jinteh Arrigo Chen , Seico Benner , Wiliam B. Dunbar , Noah A. Wilson , Kate Lieberman , Robin Abu-Shumays , Nicholas Hurt
发明人： Mark A. Akeson , David W. Deamer , Roger Jinteh Arrigo Chen , Seico Benner , Wiliam B. Dunbar , Noah A. Wilson , Kate Lieberman , Robin Abu-Shumays , Nicholas Hurt
IPC分类号： G01N27/26 , G01N27/40
CPC分类号： C12Q1/6874 , C12Q1/54 , C12Q1/6869 , C25B3/10 , G01N27/3278 , G01N27/4166 , G01N33/48721 , C12Q2522/101 , C12Q2565/631
摘要： The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. The devices and methods are also used to determine rapidly (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
摘要翻译：本文公开的本发明提供了可以检测和控制混合物中的单个聚合物在纳米孔中被另一种化合物（例如酶）作用的装置和方法。所述装置和方法还用于在反馈控制下或使用通过多核苷酸和纳米孔之间的相互作用产生的信号来快速（〜> 50Hz）确定多核苷酸的核苷酸碱基序列。本发明特别用于分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域。

7. 发明授权

US07625706B2 Methods and apparatus for characterizing polynucleotides 有权
标题翻译：表征多核苷酸的方法和装置
公开(公告)号：US07625706B2
公开(公告)日：2009-12-01
申请号：US11824949
申请日：2007-07-03
申请人： Mark Akeson , Daniel Branton , David W. Deamer , Jeffrey R. Sampson
发明人： Mark Akeson , Daniel Branton , David W. Deamer , Jeffrey R. Sampson
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： G01N33/48721 , B01L3/502707 , B01L3/502761 , B01L2300/0896 , B01L2400/0475 , B82Y5/00 , B82Y30/00 , C12Q1/6869 , C12Q2565/631 , C12Q2521/543
摘要： Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.
摘要翻译：提供了用于分析聚合物的系统和方法，例如多核苷酸。该系统能够以指定的速率分析聚合物。一个这样的分析系统包括具有纳米孔孔的结构和邻近纳米孔孔的分子马达，例如聚合酶。

8. 发明申请

US20090264621A1 Lipid-assisted synthesis of polymer compounds and methods for their use 有权
标题翻译：脂质辅助合成高分子化合物及其使用方法
公开(公告)号：US20090264621A1
公开(公告)日：2009-10-22
申请号：US12316515
申请日：2008-12-12
申请人： Sudha Rajamani , Felix Olasagasti , David W. Deamer , Seico Benner
发明人： Sudha Rajamani , Felix Olasagasti , David W. Deamer , Seico Benner
IPC分类号： C07K1/02 , C07H1/00
CPC分类号： C07H1/00 , C07K1/02
摘要： The invention herein disclosed provides for methods for the synthesis of polymers from monomers. In particular the method provides for the synthesis of polynucleotides from mononucleotides in the absence of catalytic enzymes. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
摘要翻译：本文公开的本发明提供了用于从单体合成聚合物的方法。特别地，该方法提供了在不存在催化酶的情况下从单核苷酸合成多核苷酸。本发明特别用于分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域。

9. 发明授权

US06746594B2 Miniature support for thin films containing single channels or nanopores and methods for using the same 有权
标题翻译：用于含有单通道或纳米孔的薄膜的微型载体及其使用方法
公开(公告)号：US06746594B2
公开(公告)日：2004-06-08
申请号：US09877745
申请日：2001-06-08
申请人： Mark A. Akeson , David W. Deamer , Daniel Branton
发明人： Mark A. Akeson , David W. Deamer , Daniel Branton
IPC分类号： G01N2726
CPC分类号： G01N33/48721 , C12Q1/6869 , Y10S977/781 , Y10S977/788 , Y10S977/932 , C12Q2565/631
摘要： Single-channel thin film devices and methods for using the same are provided. The subject devices comprise cis and trans chambers connected by an electrical communication means. At the cis end of the electrical communication means is a horizontal conical aperture sealed with a thin film that includes a single nanopore or channel. The devices further include a means for applying an electric field between the cis and trans chambers. The subject devices find use in applications in which the ionic current through a nanopore or channel is monitored, where such applications include the characterization of naturally occurring ion channels, the characterization of polymeric compounds, and the like.
摘要翻译：提供单通道薄膜器件及其使用方法。主体装置包括通过电通信装置连接的顺式和反式室。在电连通装置的顺式端是一个水平的圆锥形孔，其中密封有薄膜，其包括单个纳米孔或通道。该装置还包括用于在顺式和反式室之间施加电场的装置。主题装置可用于通过纳米孔或通道监测离子电流的应用，其中这些应用包括天然存在的离子通道的表征，聚合化合物的表征等。

10. 发明授权

US06267872B1 Miniature support for thin films containing single channels or nanopores and methods for using same 有权
标题翻译：用于含有单通道或纳米孔的薄膜的微型载体及其使用方法
公开(公告)号：US06267872B1
公开(公告)日：2001-07-31
申请号：US09430240
申请日：1999-10-29
申请人： Mark A. Akeson , David W. Deamer , Daniel Branton
发明人： Mark A. Akeson , David W. Deamer , Daniel Branton
IPC分类号： G01N2726
CPC分类号： G01N33/48721 , C12Q1/6869 , Y10S977/781 , Y10S977/788 , Y10S977/932 , C12Q2565/631
摘要： Single-channel thin film devices and methods for using the same are provided. The subject devices comprise cis and trans chambers connected by an electrical communication means. At the cis end of the electrical communication means is a horizontal conical aperture sealed with a thin film that includes a single nanopore or channel. The devices further include a means for applying an electric field between the cis and trans chambers. The subject devices find use in applications in which the ionic current through a nanopore or channel is monitored where such applications include the characterization of naturally occurring ion channels, the characterization of polymeric compounds, and the like.
摘要翻译：提供单通道薄膜器件及其使用方法。主体装置包括通过电通信装置连接的顺式和反式室。在电连通装置的顺式端是一个水平的圆锥形孔，其中密封有薄膜，其包括单个纳米孔或通道。该装置还包括用于在顺式和反式室之间施加电场的装置。主题装置可用于通过纳米孔或通道的离子电流被监测的应用，其中这些应用包括天然存在的离子通道的表征，聚合化合物的表征等。

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