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1. 发明授权

US06489111B1 Apparatus and methods for immobilized DNA library preparation and gene amplification 失效
标题翻译：用于固定化DNA文库制备和基因扩增的装置和方法
公开(公告)号：US06489111B1
公开(公告)日：2002-12-03
申请号：US09601973
申请日：2000-10-12
申请人： Kojiro Takahashi , Michifumi Tanga
发明人： Kojiro Takahashi , Michifumi Tanga
IPC分类号： C12Q168
CPC分类号： C12N15/1093 , B01J19/0046 , B01J2219/00313 , B01J2219/00333 , B01J2219/00495 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00659 , B01J2219/00662 , B01J2219/00677 , B01J2219/00689 , B01J2219/00722 , B01L3/50851 , B01L3/50853 , B01L7/52 , B01L2300/046 , B01L2300/0819 , B01L2300/1822 , C12Q1/6837 , C40B40/06 , C40B60/14
摘要： A purpose of the present invention is to provide a gene amplification apparatus with simple operation, wherein the apparatus can heat and cool gene rapidly and a thermal cycle can be operated in a relatively short period and a method thereof, a method for preparing immobilized DNA library suitable to the above apparatus and a method for comparing genes systematically. The apparatus for preparing immobilized DNA library according to the present invention comprises a reaction body 10 on which a grooved portion for receiving a container is formed, a cap portion 50 provided at an upper portion of the reaction body and a container 12 formed by substrates for immobilizing DNA, wherein the cap portion 50 including means for heating/cooling 51 and means for cooling 52.
摘要翻译：本发明的目的是提供一种具有简单操作的基因扩增装置，其中该装置可以快速加热和冷却基因，并且可以在相对较短的时间内进行热循环及其方法，制备固定化DNA文库的方法适用于上述装置和系统比较基因的方法。根据本发明的用于制备固定化DNA文库的装置包括反应体10，其上形成有用于容纳容器的沟槽部分，设置在反应体上部的盖部分50和由基底形成的容器12，固定DNA，其中盖部分50包括用于加热/冷却的装置51和用于冷却的装置52。

2. 发明授权

US07078517B2 Method of reusing DNA-immobilization substrate 失效
标题翻译：重复使用DNA固定底物的方法
公开(公告)号：US07078517B2
公开(公告)日：2006-07-18
申请号：US10451399
申请日：2001-12-17
申请人： Kojiro Takahashi , Osamu Takai , Michifumi Tanga
发明人： Kojiro Takahashi , Osamu Takai , Michifumi Tanga
IPC分类号： C07H23/00
CPC分类号： C07H21/00 , B01J2219/00529 , B01J2219/00608 , C40B99/00
摘要： It is intended to provide a method of reusing a DNA-immobilization substrate whereby the expensive DNA-immobilization substrate can be efficiently utilized and a reusable DNA-immobilization substrate having the same performance as a new product without any trouble in practical use can be provided. Namely, a method of reusing a DNA-immobilization substrate characterized in that, to remove DNA from a DNA-immobilization substrate carrying the DNA immobilized by an acid-amide bond via an oligonucleotide to thereby enable the immobilization of a fresh DNA, the acid-amide bond between the substrate and the DNA is hydrolyzed with an acid or an alkali.
摘要翻译：旨在提供一种重复使用DNA固定化底物的方法，由此可以有效地利用昂贵的DNA固定化底物，并且可以提供具有与新产品相同性能的可再利用的DNA固定基质，而无需实际使用中的任何麻烦。即，重复利用DNA固定基质的方法，其特征在于，为了从DNA固定化载体中除去DNA，通过寡核苷酸携带由酰胺酰胺键固定的DNA，从而能够固定新鲜的DNA，底物与DNA之间的酰胺键用酸或碱水解。

3. 发明申请

US20060024741A1 Support for fixing nucleotide and process for producing the same 审中-公开
标题翻译：支持固定核苷酸及其制备方法
公开(公告)号：US20060024741A1
公开(公告)日：2006-02-02
申请号：US11239418
申请日：2005-09-30
申请人： Michifumi Tanga , Hiroshi Okamura , Kenichi Takagi , Kojiro Takahashi
发明人： Michifumi Tanga , Hiroshi Okamura , Kenichi Takagi , Kojiro Takahashi
IPC分类号： C40B40/08 , C40B50/18
CPC分类号： C07H21/00 , C07B2200/11 , C12N15/1006 , C12Q1/6837 , C12Q2521/501 , C12Q2521/301
摘要： A support for fixing a nucleotide that contributes to the efficient clarification of DNA without damaging the terminal parts of the DNA and, therefore, is useful in the fields of biology, biochemistry and the like. This support for fixing a nucleotide is a chemically modified substrate on which oligonucleotide are immobilized characterized in that the oligonucleotide have a restriction enzyme cleavage site. This support is produced by chemically modifying the substrate, immobilizing a single-stranded oligonucleotide thereon, then hybridizing the single-stranded oligonucleotide with another single-stranded oligonucleotide having a base sequence complementary thereto, and ligating an oligonucleotide having a restriction enzyme site at the end thereof.
摘要翻译：用于固定有助于DNA的有效澄清而不损害DNA的末端部分的核苷酸的支持物，因此可用于生物学，生物化学等领域。用于固定核苷酸的这种支持是其上固定有寡核苷酸的化学修饰底物，其特征在于寡核苷酸具有限制酶切割位点。通过化学修饰底物，将单链寡核苷酸固定在其上，然后将单链寡核苷酸与其碱基序列互补的另一单链寡核苷酸杂交，并在末端连接具有限制酶位点的寡核苷酸，其中。

4. 发明授权

US07022473B1 Substrates for immobilizing and amplifying DNA and DNA-immobilized chips 失效
标题翻译：用于固定和扩增DNA和DNA固定芯片的底物
公开(公告)号：US07022473B1
公开(公告)日：2006-04-04
申请号：US09601875
申请日：1999-02-08
申请人： Michifumi Tanga , Kojiro Takahashi
发明人： Michifumi Tanga , Kojiro Takahashi
IPC分类号： C12Q1/68 , C12M1/36 , C07H21/04 , B01J29/04
CPC分类号： C12Q1/6844 , B01J2219/00274 , B01J2219/00306 , B01J2219/00497 , B01J2219/005 , B01J2219/00515 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00617 , B01J2219/00626 , B01J2219/00635 , B01J2219/00722 , C12Q1/6834 , C12Q1/686 , C12Q2565/518 , C40B40/06 , C40B60/14 , C12Q2531/113 , C12Q2527/00 , C12Q2565/537 , C12Q2521/107
摘要： Substrates for producing a DNA library are produced by immobilizing DNA onto a suitable substrate having excellent thermal conductivity. A surface of the substrate is chemically modified with a radical having a terminal polar radical. DNA can be amplified by the PCR method in a short period of time using substrates of the present invention onto which DNA is immobilized.
摘要翻译：用于制备DNA文库的底物是通过将DNA固定在具有优良导热性的合适的底物上制备的。用具有末端极性基团的基团对衬底的表面进行化学修饰。可以通过PCR方法在短时间内使用固定有DNA的本发明的底物扩增DNA。

5. 发明授权

US07029881B1 Methods for constructing DNA library and support carrying DNA library immobilized thereon 失效
标题翻译：用于构建携带其上固定有DNA文库的DNA文库和载体的方法
公开(公告)号：US07029881B1
公开(公告)日：2006-04-18
申请号：US10030619
申请日：2000-05-10
申请人： Kojiro Takahashi , Osamu Takai , Michifumi Tanga
发明人： Kojiro Takahashi , Osamu Takai , Michifumi Tanga
IPC分类号： C12P19/36
CPC分类号： C12N15/1096
摘要： Method and apparatus for constructing a cDNA library by hybridizing mRNA with oligo (dT) on a support and treating with a reverse transcriptase to immobilized complementary DNA, or for constructing a gDNA library by ligating a double-stranded chromosomal DNA library with an oligonucleotide on a support having a restriction enzyme site and then immobilizing the gDNA library.
摘要翻译：通过将mRNA与寡核苷酸（dT）杂交到载体上并用逆转录酶处理至固定的互补DNA来构建cDNA文库的方法和装置，或通过将双链染色体DNA文库与寡核苷酸连接在一起构建gDNA文库支持物具有限制酶位点，然后固定gDNA文库。

6. 发明授权

US06607908B1 Supports for immobilizing DNA or the like 失效
标题翻译：支持固定DNA等
公开(公告)号：US06607908B1
公开(公告)日：2003-08-19
申请号：US09807611
申请日：2001-07-16
申请人： Michifumi Tanga , Kojiro Takahashi
发明人： Michifumi Tanga , Kojiro Takahashi
IPC分类号： C12M134
CPC分类号： C40B99/00 , B01J19/0046 , B01J2219/00497 , B01J2219/00531 , B01J2219/00722 , B01L3/5085 , C12Q1/6834
摘要： A subject of the present invention is to provide a support with immobilized DNA as a DNA library and particularly to a support suitable for reproducing DNA in accordance with a DNA amplification reaction. In the present invention, DNA or the like is immobilized on a surface of a support made of one kind or more than material Selected from a group of a diamond including non-diamond carbon, amorphous carbon and graphite. A hydroxyl group may be bonded to a surface of the support or a group bonded to a hydrocarbon group and a carboxyl group at its terminal end may be bonded to a surface of the support through an ester linkage or a peptide linkage.
摘要翻译：本发明的主题是提供具有作为DNA文库的固定化DNA的载体，特别是提供适合于根据DNA扩增反应再生DNA的载体。在本发明中，DNA等固定在由一种或多种材料制成的支撑体的表面上，该表面由选自非金刚石碳，无定形碳和石墨的金刚石组成。羟基可以键合到载体的表面或与烃基键合的基团，并且其末端的羧基可以通过酯键或肽键键合到载体的表面。

7. 发明授权

US08802534B2 Method for forming SOI substrate and apparatus for forming the same 有权
标题翻译：用于形成SOI衬底的方法及其形成装置
公开(公告)号：US08802534B2
公开(公告)日：2014-08-12
申请号：US13488854
申请日：2012-06-05
申请人： Yoshihiro Komatsu , Tomoaki Moriwaka , Kojiro Takahashi
发明人： Yoshihiro Komatsu , Tomoaki Moriwaka , Kojiro Takahashi
IPC分类号： H01L21/331 , H01L21/8222 , H01L21/84 , H01L21/762
CPC分类号： H01L21/84 , H01L21/67092 , H01L21/7624 , H01L21/76254 , H01L27/1262 , H01L29/78654 , Y10S438/967
摘要： A bond substrate is attached with an incline toward the setting surface of a base substrate. Accordingly, an attachment starting portion can be limited. Further, the bond substrate is provided so that part of the bond substrate extends beyond a support base and the part is closest to the base substrate. Because of this, part of the bond substrate is separated from the support base with the use of an end portion of the support base as a fulcrum point because the support base is not provided below the contact portion, and attachment sequentially proceeds from a portion which gets close to the base substrate; thus, stable attachment can be performed without an air layer remaining at the interface between the bond substrate and the base substrate.
摘要翻译：接合基板朝向基底基板的设置表面倾斜地附接。因此，可以限制附件起始部分。此外，提供接合基板，使得接合基板的一部分延伸超过支撑基部，并且该部分最靠近基底。因此，由于支撑基底不设置在接触部的下方，所以借助于支撑基座的端部作为支点，将粘合基板的一部分与支撑基座分离，由此，依次从靠近基底; 因此，可以在粘合基板和基底基板之间的界面处没有空气层的情况下进行稳定的附着。

8. 发明授权

US4169669A Film winding device for slide projectors 失效
标题翻译：幻灯投影机薄膜卷绕装置
公开(公告)号：US4169669A
公开(公告)日：1979-10-02
申请号：US888986
申请日：1978-03-22
申请人： Tadakazu Endo , Susumu Nomura , Masato Matsumoto , Kojiro Takahashi
发明人： Tadakazu Endo , Susumu Nomura , Masato Matsumoto , Kojiro Takahashi
IPC分类号： G03B1/24 , G03B1/32 , G03B1/46 , G03B1/48 , G03B23/12
CPC分类号： G03B1/48 , G03B1/24 , G03B1/32 , G03B1/46 , G03B23/12 , Y10S353/02
摘要： A film winding device for slide projectors of a type using strip films comprising a film housing case capable of being removably fitted to a projector body, a film guide guiding a projected film toward the film housing case, a leading ring mounted rotatably on the end portion of the film guide and capable of leading in the projected film at the tip so as to come to the outside within the film housing case and a roller means arranged so as to be able to positively feed the projected film even in the end portion into the film housing case, whereby the film having finished being projected can be housed successively into the film housing case so as to be projectable without being rewound. In this slide projector, the film to be projected can be easily taken at the tip to the position of the film guide by pushing a button.
摘要翻译：一种用于使用带状薄膜的类型的投影机的胶片卷绕装置，其包括能够可拆卸地装配到投影机主体的胶片容纳壳体，将胶片引导器引导到胶片容纳盒的投影膜，可旋转地安装在所述端部上的引导环并且能够在尖端处引导突出的膜以便在膜容纳壳体内进入外部;以及辊装置，其布置成能够将突出的膜甚至在端部中积极地馈送到膜由此，投影完成的胶片可以依次容纳在胶卷收纳盒中，以便可以投射，而不会被重新卷绕。在这种幻灯投影机中，通过按下按钮，能够将投影的胶片容易地从胶片引导件的尖端处取出。

9. 发明授权

US5777838A Electrostatic chuck and method of attracting wafer 失效
标题翻译：静电吸盘和吸引晶圆的方法
公开(公告)号：US5777838A
公开(公告)日：1998-07-07
申请号：US752932
申请日：1996-11-20
申请人： Koki Tamagawa , Kojiro Takahashi , Takahiko Suzuki , Ryuichi Fukunishi
发明人： Koki Tamagawa , Kojiro Takahashi , Takahiko Suzuki , Ryuichi Fukunishi
IPC分类号： B23Q3/15 , C23C14/50 , H01L21/683 , H02N13/00
CPC分类号： H01L21/6875 , H01L21/6831 , H01L21/6833 , H02N13/00 , Y10T279/23
摘要： An electrostatic chuck including an electrode having first and second surfaces, a dielectric member having a first layer for covering at least the first surface of the electrode, a power supply for conducting electric power to the electrode, and a cooling gas-feeding means for feeding a cooling gas onto the surface of the first layer of the dielectric member. A plurality of fine projections 28 are formed on the surface of the first layer of the dielectric member. Each of the fine projections 28 has a tip smaller than a root thereof so as to hold a wafer W in substantially a point-contact manner. The wafer can be held on the surface of the first layer of the dielectric member and cooled by the cooling gas fed from the cooling gas-feeding means.
摘要翻译：一种静电卡盘，包括具有第一表面和第二表面的电极，具有用于至少覆盖电极的第一表面的第一层的电介质构件，用于向电极传导电力的电源以及用于馈送的冷却气体供给装置在电介质构件的第一层的表面上的冷却气体。在电介质构件的第一层的表面上形成有多个微细突起28。每个细突起28具有小于其根部的尖端，以便将晶片W基本上保持在点接触的方式。晶片可以保持在电介质构件的第一层的表面上，并由从冷却气体供给装置供给的冷却气体冷却。

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