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1. 发明授权

US06569631B1 Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes 有权
标题翻译：使用5-（4'-二甲基氨基苯基）-2-（4'-苯基）恶唑衍生物荧光染料的配体发育的微板热转移测定
公开(公告)号：US06569631B1
公开(公告)日：2003-05-27
申请号：US09438357
申请日：1999-11-12
申请人： Michael W. Pantoliano , Eugenio C. Petrella , F. Raymond Salemme , Barry A. Springer
发明人： Michael W. Pantoliano , Eugenio C. Petrella , F. Raymond Salemme , Barry A. Springer
IPC分类号： G01N3353
CPC分类号： G01N33/582 , G01N33/5375
摘要： The present invention provides a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of unfolding due to a thermal change. The method comprises (a) contacting the target molecule with one molecule of a multiplicity of different molecules, in the presence of a 5-(4″-dimethylaminopheny-2-(4′-phenyl)oxazole derivative dye, in each of a multiplicity of containers; (b) simultaneously heating the multiplicity of containers; (c) measuring the fluorescence in each of the containers; (d) generating thermal unfolding information for the target molecule as a function of temperature for each of the containers; (e) comparing the thermal unfolding information obtained for each of the containers to the thermal unfolding information for the target molecule in the absence of any of the molecules in the multiplicity of different molecules; and (f) ranking the affinities of each of the molecules according to the difference in the thermal unfolding information between the target molecule in each of the containers and the target molecule in the absence of any of the molecules in the multiplicity of different molecules.
摘要翻译：本发明提供了一种用于对能够由于热变化而展开的靶分子的多种不同分子的亲和力进行排序的方法。该方法包括（a）在5-（4“ - 二甲基氨基苯基-2-（4'-苯基）恶唑衍生物染料存在下，将目标分子与多个不同分子的一个分子接触，容器的多样性;（b）同时加热多个容器;（c）测量每个容器中的荧光;（d）作为每个容器的温度的函数产生靶分子的热展开信息;（e ）在不存在多个不同分子中的任何分子的情况下将每个容器获得的热解折叠信息与目标分子的热解折叠信息进行比较;以及（f）根据在不同分子中多个分子中没有任何分子的情况下，每个容器中的目标分子与靶分子之间的热展开信息的差异。

2. 发明授权

US06268158B1 Method for determining conditions that facilitate protein crystallization 有权
标题翻译：确定促进蛋白质结晶的条件的方法
公开(公告)号：US06268158B1
公开(公告)日：2001-07-31
申请号：US09458663
申请日：1999-12-10
申请人： Michael W. Pantoliano , Eugenio C. Petrella , Francis R. Salemme , Barry A. Springer
发明人： Michael W. Pantoliano , Eugenio C. Petrella , Francis R. Salemme , Barry A. Springer
IPC分类号： G01N3353
CPC分类号： C40B30/04 , B01L7/00 , B01L7/52 , C07K1/047 , C12Q1/6811 , G01N33/68 , G01N33/6803 , G01N33/6845 , G01N33/86 , G01N35/028 , G01N2500/00
摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.
摘要翻译：本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到从每个容器中的加热，产生作为每个容器的温度的函数的目标分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。

3. 发明授权

US06291192B1 Method for identifying conditions that facilitate recombinant protein folding 有权
标题翻译：用于鉴定促进重组蛋白质折叠的条件的方法
公开(公告)号：US06291192B1
公开(公告)日：2001-09-18
申请号：US09477724
申请日：2000-01-05
申请人： Michael W. Pantoliano , Eugenio C. Petrella , Francis R. Salemme , Barry A. Springer
发明人： Michael W. Pantoliano , Eugenio C. Petrella , Francis R. Salemme , Barry A. Springer
IPC分类号： G01N3353
CPC分类号： C40B30/04 , B01L7/00 , B01L7/52 , C07K1/047 , C12Q1/6811 , G01N33/68 , G01N33/6803 , G01N33/6845 , G01N33/86 , G01N35/028 , G01N2500/00
摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.
摘要翻译：本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到从每个容器中的加热，产生作为每个容器的温度的函数的靶分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。

4. 发明授权

US06291191B1 Microplate thermal shift assay for ligand development and multi-variable protein chemistry optimization 有权
标题翻译：用于配体开发和多变量蛋白质化学优化的微板热转移测定
公开(公告)号：US06291191B1
公开(公告)日：2001-09-18
申请号：US09452203
申请日：1999-12-02
申请人： Michael W. Pantoliano , Eugenio C. Petrella , Alexander W. Rhind , Francis R. Salemme , Barry A. Springer
发明人： Michael W. Pantoliano , Eugenio C. Petrella , Alexander W. Rhind , Francis R. Salemme , Barry A. Springer
IPC分类号： G01N3353
CPC分类号： C40B30/04 , B01L7/00 , B01L7/52 , C07K1/047 , C12Q1/6811 , G01N33/68 , G01N33/6803 , G01N33/6845 , G01N33/86 , G01N35/028 , G01N2500/00
摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.
摘要翻译：本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到从每个容器中的加热，产生作为每个容器的温度的函数的靶分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。

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