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1. 发明授权

US06291191B1 Microplate thermal shift assay for ligand development and multi-variable protein chemistry optimization 有权
标题翻译：用于配体开发和多变量蛋白质化学优化的微板热转移测定
公开(公告)号：US06291191B1
公开(公告)日：2001-09-18
申请号：US09452203
申请日：1999-12-02
申请人： Michael W. Pantoliano , Eugenio C. Petrella , Alexander W. Rhind , Francis R. Salemme , Barry A. Springer
发明人： Michael W. Pantoliano , Eugenio C. Petrella , Alexander W. Rhind , Francis R. Salemme , Barry A. Springer
IPC分类号： G01N3353
CPC分类号： C40B30/04 , B01L7/00 , B01L7/52 , C07K1/047 , C12Q1/6811 , G01N33/68 , G01N33/6803 , G01N33/6845 , G01N33/86 , G01N35/028 , G01N2500/00
摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.
摘要翻译：本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到从每个容器中的加热，产生作为每个容器的温度的函数的靶分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。

2. 发明授权

US06214293B1 Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization 有权
标题翻译：用于配体开发和多变量蛋白质化学优化的微板热转移测定装置
公开(公告)号：US06214293B1
公开(公告)日：2001-04-10
申请号：US09459996
申请日：1999-12-14
申请人： Michael W. Pantoliano , Roger F. Bone , Alexander W. Rhind , Francis R. Salemme
发明人： Michael W. Pantoliano , Roger F. Bone , Alexander W. Rhind , Francis R. Salemme
IPC分类号： G01N2164
CPC分类号： C40B30/04 , B01L7/00 , B01L7/52 , C07K1/047 , C12Q1/6811 , G01N33/68 , G01N33/6803 , G01N33/6845 , G01N33/86 , G01N35/028 , G01N2500/00
摘要： The present invention provides an assay apparatus for that includes a temperature adjusting means for simultaneously heating a plurality of samples, and a receiving means for receiving spectral emission from the samples while the samples are being heated. In further aspects of the invention, the receiving means can be configured to receive fluorescent emission, ultraviolet light, and visible light. The receiving means can be configured to receive spectral emission from the samples in a variety of ways, e.g., one sample at a time, simultaneously from more than one sample, or simultaneously from all of the samples. The temperature adjusting means can be configured with a temperature controller for changing temperature in accordance with a pre-determined profile.
摘要翻译：本发明提供一种用于同时加热多个样品的温度调节装置和用于在样品被加热时从样品接收光谱发射的接收装置的测定装置。在本发明的另外的方面，接收装置可以被配置为接收荧光发射，紫外光和可见光。接收装置可以被配置为以多种方式从样本接收光谱发射，例如一次一个样本，同时来自多于一个样本，或同时来自所有样本。温度调节装置可以配置有用于根据预定轮廓改变温度的温度控制器。

3. 发明授权

US6036920A Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization 失效
标题翻译：用于配体开发和多变量蛋白质化学优化的微板热转移测定装置
公开(公告)号：US6036920A
公开(公告)日：2000-03-14
申请号：US853459
申请日：1997-05-09
申请人： Michael W. Pantoliano , Roger F. Bone , Alexander W. Rhind , Francis R. Salemme
发明人： Michael W. Pantoliano , Roger F. Bone , Alexander W. Rhind , Francis R. Salemme
IPC分类号： C12N15/00 , B01L7/00 , C07K1/04 , C07K2/00 , C12Q1/68 , C40B30/04 , G01N33/48 , G01N33/50 , G01N33/53 , G01N33/68 , G01N33/86 , G01N35/02 , G01N21/64
CPC分类号： C40B30/04 , B01L7/00 , B01L7/52 , C07K1/047 , C12Q1/6811 , G01N33/68 , G01N33/6803 , G01N33/6845 , G01N33/86 , G01N35/028 , G01N2500/00
摘要： The present invention provides an assay apparatus for that includes a temperature adjusting device for simultaneously heating a plurality of samples, and a receiving device for receiving spectral emission from the samples while the samples are being heated. In further aspects of the invention, the receiving device can be configured to receive fluorescent emission, ultraviolet light, and visible light. The receiving device can be configured to receive spectral emission from the samples in a variety of ways, e.g., one sample at a time, simultaneously from more than one sample, or simultaneously from all of the samples. The temperature adjusting device can be configured with a temperature controller for changing temperature in accordance with a pre-determined profile.
摘要翻译：本发明提供了一种用于同时加热多个样品的温度调节装置和用于在样品被加热时从样品接收光谱发射的接收装置的测定装置。在本发明的其它方面，接收装置可被配置为接收荧光发射，紫外光和可见光。接收装置可以被配置为以多种方式从样本接收频谱发射，例如一次一个样本，同时来自多于一个样本，或同时来自所有样本。温度调节装置可以配置有用于根据预定轮廓改变温度的温度控制器。

4. 发明授权

US06303322B1 Method for identifying lead compounds 有权
标题翻译：铅化合物鉴定方法
公开(公告)号：US06303322B1
公开(公告)日：2001-10-16
申请号：US09478216
申请日：2000-01-05
申请人： Michael W. Pantoliano , Alexander W. Rhind , Francis R. Salemme
发明人： Michael W. Pantoliano , Alexander W. Rhind , Francis R. Salemme
IPC分类号： G01N3353
CPC分类号： C40B30/04 , B01L7/00 , B01L7/52 , C07K1/047 , C12Q1/6811 , G01N33/68 , G01N33/6803 , G01N33/6845 , G01N33/86 , G01N35/028 , G01N2500/00
摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.
摘要翻译：本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到从每个容器中的加热，产生作为每个容器的温度的函数的目标分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。

5. 发明授权

US06268218B1 Method for sensing and processing fluorescence data from multiple samples 有权
标题翻译：用于感测和处理来自多个样品的荧光数据的方法
公开(公告)号：US06268218B1
公开(公告)日：2001-07-31
申请号：US09459997
申请日：1999-12-14
申请人： Michael W. Pantoliano , Roger F. Bone , Alexander W. Rhind , Francis R. Salemme
发明人： Michael W. Pantoliano , Roger F. Bone , Alexander W. Rhind , Francis R. Salemme
IPC分类号： G01N2164
CPC分类号： C40B30/04 , B01L7/00 , B01L7/52 , C07K1/047 , C12Q1/6811 , G01N33/68 , G01N33/6803 , G01N33/6845 , G01N33/86 , G01N35/028 , G01N2500/00
摘要： The present invention provides an assay apparatus for that includes a temperature adjusting device for simultaneously heating a plurality of samples, and a receiving device for receiving spectral emission from the samples while the samples are being heated. In further aspects of the invention, the receiving device can be configured to receive fluorescent emission, ultraviolet light, and visible light. The receiving device can be configured to receive spectral emission from the samples in a variety of ways, e.g., one sample at a time, simultaneously from more than one sample, or simultaneously from all of the samples. The temperature adjusting device can be configured with a temperature controller for changing temperature in accordance with a pre-determined profile.
摘要翻译：本发明提供了一种用于同时加热多个样品的温度调节装置和用于在样品被加热时从样品接收光谱发射的接收装置的测定装置。在本发明的其它方面，接收装置可被配置为接收荧光发射，紫外光和可见光。接收装置可以被配置为以多种方式从样本接收频谱发射，例如一次一个样本，同时来自多于一个样本，或同时来自所有样本。温度调节装置可以配置有用于根据预定轮廓改变温度的温度控制器。

6. 发明授权

US06849458B2 Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization 有权
标题翻译：用于配体开发和多变量蛋白质化学优化的微板热转移测定装置
公开(公告)号：US06849458B2
公开(公告)日：2005-02-01
申请号：US09801676
申请日：2001-03-09
申请人： Michael W. Pantoliano , Roger F. Bone , Alexander W. Rhind , Francis R. Salemme
发明人： Michael W. Pantoliano , Roger F. Bone , Alexander W. Rhind , Francis R. Salemme
IPC分类号： C12N15/00 , B01L7/00 , C07K1/04 , C07K2/00 , C12Q1/68 , C40B30/04 , G01N33/48 , G01N33/50 , G01N33/53 , G01N33/68 , G01N33/86 , G01N35/02
CPC分类号： C40B30/04 , B01L7/00 , B01L7/52 , C07K1/047 , C12Q1/6811 , G01N33/68 , G01N33/6803 , G01N33/6845 , G01N33/86 , G01N35/028 , G01N2500/00
摘要： The present invention provides an assay apparatus for that includes a temperature adjusting means for simultaneously heating a plurality of samples, and a receiving means for receiving spectral emission from the samples while the samples are being heated. In further aspects of the invention, the receiving means can be configured to receive fluorescent emission, ultraviolet light, and visible light. The receiving means can be configured to receive spectral emission from the samples in a variety of ways, e.g., one sample at a time, simultaneously from more than one sample, or simultaneously from all of the samples. The temperature adjusting means can be configured with a temperature controller for changing temperature in accordance with a predetermined profile.
摘要翻译：本发明提供一种用于同时加热多个样品的温度调节装置和用于在样品被加热时从样品接收光谱发射的接收装置的测定装置。在本发明的另外的方面，接收装置可以被配置为接收荧光发射，紫外光和可见光。接收装置可以被配置为以多种方式从样本接收光谱发射，例如一次一个样本，同时来自多于一个样本，或同时来自所有样本。温度调节装置可以配置有用于根据预定轮廓改变温度的温度控制器。

7. 发明授权

US06020141A Microplate thermal shift assay for ligand development and multi-variable protein chemistry optimization 失效
标题翻译：用于配体开发和多变量蛋白质化学优化的微板热转移测定
公开(公告)号：US06020141A
公开(公告)日：2000-02-01
申请号：US853464
申请日：1997-05-09
申请人： Michael W. Pantoliano , Alexander W. Rhind , Francis R. Salemme
发明人： Michael W. Pantoliano , Alexander W. Rhind , Francis R. Salemme
IPC分类号： C12N15/00 , B01L7/00 , C07K1/04 , C07K2/00 , C12Q1/68 , C40B30/04 , G01N33/48 , G01N33/50 , G01N33/53 , G01N33/68 , G01N33/86 , G01N35/02 , C12Q1/00
CPC分类号： C40B30/04 , B01L7/00 , B01L7/52 , C07K1/047 , C12Q1/6811 , G01N33/68 , G01N33/6803 , G01N33/6845 , G01N33/86 , G01N35/028 , G01N2500/00
摘要： The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (T.sub.m) therefrom, comparing the T.sub.m of each of the thermal denaturation curves with the T.sub.m of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in T.sub.m of each of the thermal denaturation curves.
摘要翻译：本发明是一种用于对能够由于热变化而变性的靶分子的多种不同分子的亲和力进行排序的方法。该方法包括使目标分子与多个不同分子中的多个不同分子的一个分子接触，同时加热多个容器，在每个容器中测量与靶分子的热变性相关的物理变化，得到从每个容器中的加热，产生作为每个容器的温度的函数的目标分子的热变性曲线，并从其中确定中点温度（Tm），将每个热变性曲线的Tm与在不存在多个不同分子中的任何分子的情况下，针对靶分子获得的热变性曲线的Tm，并根据每个热变性曲线的Tm的变化对不同分子的多样性的亲和力进行排序。

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