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1. 发明申请

US20050135974A1 Device for preparing multiple assay samples using multiple array surfaces 审中-公开
标题翻译：使用多个阵列表面制备多个测定样品的装置
公开(公告)号：US20050135974A1
公开(公告)日：2005-06-23
申请号：US10739784
申请日：2003-12-18
申请人： Michael Harvey , Lara Dowland , Breck Parker , Stephen Sherman , To Cheung , Bin Li , Yong Peng , Qi- Cai , Ke Liu , Hai Ge , Fu Deng , Li Ding
发明人： Michael Harvey , Lara Dowland , Breck Parker , Stephen Sherman , To Cheung , Bin Li , Yong Peng , Qi- Cai , Ke Liu , Hai Ge , Fu Deng , Li Ding
IPC分类号： B01L3/00 , B01L9/00 , G01N1/31 , G01N31/22
CPC分类号： B01L3/50855 , B01L9/52 , B01L2200/025 , B01L2300/0822 , B01L2300/0829 , G01N1/312
摘要： The present invention relates to a device for preparing assay samples using a number of microscope slides. Each slide has a number of assay reaction surface locations spaced on the planar surface of the slide. In preferred embodiments, the device comprises, in part, a microscope slide holder that has the exterior dimensions of a SBS standard microplate, such as a 96 well plate. The device accepts conventional microscope slides equipped with sixteen microarray surfaces spaced nine millimeters apart on center, or four for a 96 well plate. Individual chamber plates are placed on top of the slides, creating an individual well above each assay reaction surface location. In preferred embodiments, each assay reaction surface location can comprise a microarray of multiple reactive sites. Thus, parallel processing can be done of samples for genomic or proteomic profiling. An advantage of the present invention is that one can use the conventional high throughput assaying equipment for SBS standard microplates while using conventional microscope slides, thereby allowing the use of robotic assay reading equipment designed for slides.
摘要翻译：本发明涉及使用多个显微镜幻灯片制备测定样品的装置。每个载片具有在载玻片平面上间隔开的多个测定反应表面位置。在优选实施例中，该装置部分地包括具有诸如96孔板的SBS标准微板的外部尺寸的显微镜载玻片保持器。该装置接受传统的显微镜载玻片，其中装有十六个微阵列表面，中心隔开9毫米，或四个96孔板。单独的室板放置在载玻片的顶部，在每个测定反应表面位置之上产生单个孔。在优选实施方案中，每个测定反应表面位置可以包含多个反应性位点的微阵列。因此，可以对基因组或蛋白质组图谱的样品进行并行处理。本发明的一个优点是可以使用传统的高通量测定设备用于SBS标准微孔板，同时使用传统的显微镜载玻片，从而允许使用为幻灯片设计的机器人测定读数设备。

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