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    • 3. 发明申请
    • Electron induced fluorescence for nucleic acid sequencing
    • 用于核酸测序的电子诱导荧光
    • US20050019800A1
    • 2005-01-27
    • US10859879
    • 2004-06-02
    • Eric Hannah
    • Eric Hannah
    • C07H21/00C12Q1/68G01N27/447C12P19/34
    • C07H21/00C12Q1/6874G01N27/44721G01N27/44726C12Q2565/629
    • An apparatus, compositions and related methods for sequencing a target nucleic acid are described. In certain embodiments, the apparatus is a microfluidic apparatus comprising an input chamber, microchannel, output chamber and a detection unit that is operatively connected to the microchannel. In preferred embodiments, the methods include hybridizing a target nucleic acid to one or more probe libraries, moving the hybridized target nucleic acid past the detector, and detecting bound probes. Probe libraries may comprise oligonucleotides or oligonucleotide analogs, preferably with each probe uniquely labeled. A linear order of labeled probes hybridized to the target nucleic acid can be detected and the target nucleic acid sequence deduced. In preferred embodiments, probe labels are detected by analysis of electron-induced fluorescence of probes labeled with conductive polymers.
    • 描述了用于测序靶核酸的装置,组合物和相关方法。 在某些实施例中,该装置是包括输入室,微通道,输出室和可操作地连接到微通道的检测单元的微流体装置。 在优选的实施方案中,所述方法包括将目标核酸与一个或多个探针文库杂交,使杂交的靶核酸通过检测器移动,并检测结合的探针。 探针文库可以包含寡核苷酸或寡核苷酸类似物,优选每个探针唯一标记。 可以检测与靶核酸杂交的标记探针的线性顺序,并推导出靶核酸序列。 在优选实施方案中,通过用导电聚合物标记的探针的电子诱导荧光的分析来检测探针标记。