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1. 发明专利

JP2008206519A Novel glyphosate n-acetyltransferase (gat) gene 审中-公开
标题翻译：新型谷氨酸N-乙酰基转移酶（GAT）基因
公开(公告)号：JP2008206519A
公开(公告)日：2008-09-11
申请号：JP2008051411
申请日：2008-02-29
申请人： Maxygen Inc , Pioneer Hi-Bred Internatl Inc , パイオニアハイ−ブレッドインターナショナル, インコーポレイテッド , マキシジェン, インコーポレイテッド
发明人： CASTLE LINDA A , SIEHL DAN , GIVER LORRAINE J , MINSHULL JEREMY , IVY CHRISTINA , CHEN YONG HONG , DUCK NICHOLAS B , MCCUTCHEN BILLY F , KEMBLE ROGER , PATTEN PHILLIP A
IPC分类号： A01H5/00 , C12N15/09 , C12N5/10 , C12N9/10 , C12N15/54 , C12N15/82
CPC分类号： C12N9/1029 , C12N15/8209 , C12N15/8275
摘要： PROBLEM TO BE SOLVED: To provide a method and reagent for making a living body (e.g. a plant) as resistant against Glyphosate. SOLUTION: This polynucleotide which is an isolated polynucleotide or a recombinant polynucleotide, contains the following: (a) a sequence selected from a group consisting of specific sequences and nucleotide sequences encoding amino acid sequences capable of being optimally arranged so as to produce at least 430 resemble scores by using a BLOSUM62 matrix, 11 gap existence penalties and one gap extension penalty; or (b) their complemental chain nucleotide sequences. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供用于制造抗草甘膦的生物体（例如植物）的方法和试剂。解决方案：作为分离的多核苷酸或重组多核苷酸的多核苷酸包含以下物质：（a）选自特定序列的序列和编码能够最佳排列以产生的氨基酸序列的核苷酸序列通过使用BLOSUM62矩阵至少有430个得分，11个差距存在罚则和一个差距延伸罚分; 或（b）其互补链核苷酸序列。版权所有（C）2008，JPO＆INPIT

2. 发明专利

JP2010220617A New glyphosate-n-acetyltransferase(gat) gene 审中-公开
标题翻译：新型谷氨酸-N-乙酰基转移酶（GAT）基因
公开(公告)号：JP2010220617A
公开(公告)日：2010-10-07
申请号：JP2010106022
申请日：2010-04-30
申请人： E I Du Pont De Nemours & Co , Pioneer Hi-Bred Internatl Inc , Verdia Inc , イー.アイ. デュポンデヌムールアンドカンパニー , ヴァーディア,インコーポレイテッド , パイオニアハイ−ブレッドインターナショナル, インコーポレイテッド
发明人： CASTLE LINDA A , SIEHL DAN , GIVER LORRAINE J , MINSHULL JEREMY , IVY CHRISTINA , CHEN YONG HONG , PATTEN PHILLIP A , GORTON REBECCA , DUCK NICHOLAS B , MCCUTCHEN BILLY FRED , KEMBLE ROGER
IPC分类号： C12N5/10 , A01H5/00 , A01N25/00 , A01N47/36 , A01N57/20 , A01P13/00 , C12N9/10 , C12N15/09 , C12N15/82
CPC分类号： C12N9/1029 , C12N15/8275
摘要： PROBLEM TO BE SOLVED: To provide new polynucleotides and polypeptides imparting resistance to herbicides. SOLUTION: New proteins are provided herein, including proteins catalyzing the acetylation of glyphosate and other structurally related proteins. Also there are provided new polynucleotides encoding these proteins, compositions that include one or more of these new proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these new compounds, diversification methods relating the new compounds, and methods of using the compounds. Some of the new methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供赋予除草剂抗性的新的多核苷酸和多肽。解决方案：本文提供了新的蛋白质，包括催化草甘膦和其他结构相关蛋白质乙酰化的蛋白质。还提供了编码这些蛋白质的新的多核苷酸，包括这些新蛋白质和/或多核苷酸中的一种或多种的组合物，包含这些新化合物的重组细胞和转基因植物，与新化合物相关的多样化方法以及使用该化合物的方法。本文提供的一些新方法和化合物可以用于产生对草甘膦有抗性的生物，如植物。版权所有（C）2011，JPO＆INPIT

3. 发明专利

JP2010227104A New glyphosate-n-acetyltransferase (gat) gene 审中-公开
标题翻译：新型谷氨酸-N-乙酰基转移酶（GAT）基因
公开(公告)号：JP2010227104A
公开(公告)日：2010-10-14
申请号：JP2010106023
申请日：2010-04-30
申请人： E I Du Pont De Nemours & Co , Pioneer Hi-Bred Internatl Inc , Verdia Inc , イー.アイ. デュポンデヌムールアンドカンパニー , ヴァーディア,インコーポレイテッド , パイオニアハイ−ブレッドインターナショナル, インコーポレイテッド
发明人： CASTLE LINDA A , SIEHL DAN , GIVER LORRAINE J , MINSHULL JEREMY , IVY CHRISTINA , CHEN YONG HONG , PATTEN PHILLIP A , GORTON REBECCA , DUCK NICHOLAS B , MCCUTCHEN BILLY FRED , KEMBLE ROGER
IPC分类号： C12N15/09 , A01H5/00 , C07K14/415 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12N15/82
CPC分类号： C12N9/1029 , C12N15/8275
摘要： PROBLEM TO BE SOLVED: To provide new polynucleotides and polypeptides imparting resistance to herbicides. SOLUTION: New proteins are provided herein, including proteins catalyzing the acetylation of glyphosate and other structurally related proteins. Also there are provided new polynucleotides encoding these proteins, compositions that include one or more of these new proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these new compounds, diversification methods relating the new compounds, and methods of using the compounds. Some of the new methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供赋予除草剂抗性的新的多核苷酸和多肽。解决方案：本文提供了新的蛋白质，包括催化草甘膦和其他结构相关蛋白质乙酰化的蛋白质。还提供了编码这些蛋白质的新的多核苷酸，包括这些新蛋白质和/或多核苷酸中的一种或多种的组合物，包含这些新化合物的重组细胞和转基因植物，与新化合物相关的多样化方法以及使用该化合物的方法。本文提供的一些新方法和化合物可以用于产生对草甘膦有抗性的生物，如植物。版权所有（C）2011，JPO＆INPIT

4. 发明专利

JP2010142234A New glyphosate n-acetyltransferase (gat) gene 审中-公开
标题翻译：新型谷氨酸N-乙酰基转移酶（GAT）基因
公开(公告)号：JP2010142234A
公开(公告)日：2010-07-01
申请号：JP2009299177
申请日：2009-12-29
申请人： E I Du Pont De Nemours & Co , Pioneer Hi-Bred Internatl Inc , Verdia Inc , イー. アイ. ドゥポンデュヌムールアンドカンパニー , ヴァーディア,インコーポレイテッド , パイオニアハイ−ブレッドインターナショナル, インコーポレイテッド
发明人： CASTLE LINDA A , SIEHL DAN , GIVER LORRAINE J , MINSHULL JEREMY , IVY CHRISTINA , CHEN YONG HONG , DUCK NICHOLAS B , MCCUTCHEN BILLY F , KEMBLE ROGER , PATTEN PHILLIP A
IPC分类号： A01H5/00 , C12N15/09 , A01H1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12N15/54 , C12N15/82 , C12Q1/04
CPC分类号： C12N9/1029 , C12N15/8209 , C12N15/8275
摘要： PROBLEM TO BE SOLVED: To provide a method and reagent for making a living body (e.g. a plant) resistant to glyphosate. SOLUTION: This new glyphosate N-acetyltransferase (GAT) gene is an isolated polynucleotide or a recombinant polynucleotide, and contains the following: (a) a sequence selected from the group consisting of a plurality of specific amino acid sequences and nucleotide sequences encoding the amino acid sequences capable of being optimally aligned in an order by using a BLOSUM62 matrix, 11 gap existence penalties and 1 gap extension penalty so as to produce at least 430 similarity scores, or (b) complementary chain nucleotide sequences to the above. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供一种制造对草甘膦具有抗性的活体（如植物）的方法和试剂。解决方案：这种新的草甘膦N-乙酰转移酶（GAT）基因是分离的多核苷酸或重组多核苷酸，并且包含以下：（a）选自多个特定氨基酸序列和核苷酸序列的序列编码能够通过使用BLOSUM62基质顺序优化的氨基酸序列，11间隙存在惩罚和1个间隙延伸罚分，以产生至少430个相似性得分，或（b）上述互补链核苷酸序列。版权所有（C）2010，JPO＆INPIT

5. 发明专利

JP2010178757A Oligonucleotide-mediated nucleic acid recombination 审中-公开
标题翻译：寡核苷酸介导的核酸重组
公开(公告)号：JP2010178757A
公开(公告)日：2010-08-19
申请号：JP2010095500
申请日：2010-04-16
申请人： Maxygen Inc , マキシジェン, インコーポレイテッド
发明人： CRAMERI ANDREAS , STEMMER WILLEM P C , MINSHULL JEREMY , BASS STEVEN H , WELCH MARK , NESS JON E , GUSTAFSSON CLAES , PATTEN PHILIP A
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method for recombining nucleic acids including homologous nucleic acids, families of gene shuffling oligonucleotides, their use in a recombination procedure, and a polymerase- and ligase-mediated recombination method. SOLUTION: There is provided a method for oligonucleotide-assisted shuffling of nucleic acids. These oligonucleotide-assisted approaches especially facilitate a family shuffling procedure and provide an substantially simplified shuffling protocol. The protocol can be used for forming a family-shuffled nucleic acid without isolating or cloning whole-length homologous nucleic acids. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供重组核酸的方法，包括同源核酸，基因改组寡核苷酸家族，其在重组过程中的用途，以及聚合酶和连接酶介导的重组方法。解决方案：提供了核酸的寡核苷酸辅助改组方法。这些寡核苷酸辅助方法特别促进了家族洗牌过程并提供了基本上简化的洗牌方案。该方案可用于形成家族洗牌的核酸而不分离或克隆全长同源的核酸。版权所有（C）2010，JPO＆INPIT

6. 发明专利

JP2010131014A Evolution of whole cells and organisms by recursive sequence recombination 审中-公开
标题翻译：通过重新序列重组实现全细胞和有机体的发育
公开(公告)号：JP2010131014A
公开(公告)日：2010-06-17
申请号：JP2009288163
申请日：2009-12-18
申请人： Maxygen Inc , マキシジェン, インコーポレイテッド
发明人： DEL CARDAYRE STEPHEN , TOBIN MATTHEW , STEMMER WILLEM P C , NESS JON E , MINSHULL JEREMY , PATTEN PHILIP A , SUBRAMANIAN VENKITESWATAN , CASTLE LINDA A , KREBBER CLAUS M , BASS STEVE , ZHANG YING-XIN , COX TONY , HUISMAN GJALT , YUAN LING , AFFHOLTER JOSEPH A
IPC分类号： A01H1/00 , A01H5/00 , A01K67/02 , A01K67/027 , C07K14/245 , C12N1/19 , C12N5/10 , C12N15/09 , C12N15/10 , C12N15/82 , C12P17/16 , C12Q1/68
CPC分类号： C12N15/102 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12P17/162 , C12Q1/68 , C12Q1/6811 , C12Q1/6827 , C12R1/465
摘要： PROBLEM TO BE SOLVED: To provide a novel and a large number of molecular genetic methods and compositions for evolution of whole and partial genomes. SOLUTION: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties (including enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites). For example, methods includes the steps of fertilizing pooled male-female gametes derived from different species, growing it up to be a reproducibly survival organism, preparing an organism library of various multicellular organisms by repeating the mating process, and selecting an organism having a desired trait or property. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供用于全部和部分基因组进化的新颖和大量的分子遗传方法和组合物。解决方案：本发明提供了采用迭代循环重复和选择/筛选整个细胞和生物体以获得所需性质（包括增强的重组原型，基因组拷贝数以及蛋白质表达和/或分泌能力的方法）的方法，次级代谢物）。例如，方法包括以下步骤：对来自不同物种的合并雄性 - 雌性配子进行施肥，将其生长成可重复存活的生物，通过重复交配过程制备各种多细胞生物的生物文库，并选择具有所需的生物体特征或财产。版权所有（C）2010，JPO＆INPIT

7. 发明专利

JP2009277235A Method, system, and software for identifying functional bio-molecule 审中-公开
标题翻译：用于鉴定功能生物分子的方法，系统和软件
公开(公告)号：JP2009277235A
公开(公告)日：2009-11-26
申请号：JP2009123638
申请日：2009-05-21
申请人： Maxygen Inc , マキシジェン, インコーポレイテッド
发明人： GUSTAFSSON CLAES , GOVINDARAJAN SRIDAR , EMIG ROBIN , FOX RICHARD JOHN , ROY AJOY , MINSHULL JEREMY , DAVIS S CHRISTOPHER , COX ANTHONY , PATTEN PHIL , CASTLE LINDA A , SIEHL DANIEL L , GORTON REBECCA LYNNE , CHEN TEDDY
IPC分类号： C12N15/09 , G01N33/48 , G01N33/50 , G01N33/53 , G06F19/18
CPC分类号： C40B50/02 , C40B30/02 , G06F19/18 , G06F19/24
摘要： PROBLEM TO BE SOLVED: To provide new methods of efficiently searching sequence space for identifying functional protein. SOLUTION: The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供有效搜索序列空间以鉴定功能性蛋白质的新方法。解决方案：本发明一般涉及快速有效地搜索生物相关数据空间的方法。更具体地，本发明包括从复杂的生物分子文库或这些文库集合鉴定具有所需性质或最适合于获得这些性质的生物分子的方法。本发明还提供了对序列活性关系建模的方法。由于许多方法是计算机实现的，本发明另外提供用于执行这些方法的数字系统和软件。版权所有（C）2010，JPO＆INPIT

8. 发明专利

JP2001197895A METHOD AND COMPOSITION FOR CELL TECHNOLOGY AND METABOLISM TECHNOLOGY 失效
公开(公告)号：JP2001197895A
公开(公告)日：2001-07-24
申请号：JP2000379523
申请日：2000-12-13
申请人： MAXYGEN INC
发明人： MINSHULL JEREMY , STEMMER WILLEM P C
IPC分类号： C12N15/09 , C07K14/11 , C07K14/435 , C07K14/545 , C07K16/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/38 , C12N9/86 , C12N15/10 , C12N15/52 , C12N15/64 , C12Q1/02 , C12Q1/68 , C40B40/02
摘要： PROBLEM TO BE SOLVED: To provide a method for evolving a new metabolic pathway and increasing a bioprocess. SOLUTION: This method is method for evloving the new metabolic pathway comprising the process generally called circulative sequence recombination in this disclosure and increasing the bioprocess. The circulative sequence recombination necessitates to carry out the repetitive cycle of recombination and screening or selection to evolve individual gene, whole plasmid or virus, multigene cluster or even whole genome. The technics like this necessitates no wide range of analysis and calculation needed in the conventional method on the metabolism technology.

9. 发明申请

WO0173000A3 METHODS FOR MODULATING CELLULAR AND ORGANISMAL PHENOTYPES 审中-公开
标题翻译：调节细胞和有机基因的方法
公开(公告)号：WO0173000A3
公开(公告)日：2002-06-27
申请号：PCT/US0109203
申请日：2001-03-23
申请人： MAXYGEN INC , STEMMER WILLEM P C , MINSHULL JEREMY , KEENAN ROBERT J
发明人： STEMMER WILLEM P C , MINSHULL JEREMY , KEENAN ROBERT J
IPC分类号： C12N15/10 , C12N15/113 , C12N15/66 , C12N15/11 , C12N15/63 , C12Q1/68
CPC分类号： C12N15/1079 , C07K2319/00 , C12N15/1027 , C12N15/1034 , C12N15/113 , C12N15/66 , C12N2310/111
摘要： Methods for identifying and controlling the genetic and metabolic pathways underlying complex phenotypes are provided. Conjoint polynucleotide segments that contribute to or disrupt elements of a multigenic phenotype are produced and expressed in cells of interest. Conjoint polynucleotide segments are recombined and/or mutated to give rise to libraries of recombinant concatamers which are expressed in cells of interest. Libraries of conjoint polynucleotide segments and recombinant concatamers are expressed episomally or integrated into the DNA of organelles or chromosomes. Cells are screened or selected to identify members of the population of cells exhibiting a desired phenotype. Libraries and vectors comprising conjoint polynucleotide segments and recombinant concatamers, as well as cells expressing such libraries and vectors or their components are provided. Kits containing conjoint polynucleotide segments, recombinant concatamers, vectors including such polynucleotides, and cells including such polynucleotides and vectors are provided.
摘要翻译：提供了鉴定和控制复杂表型下遗传和代谢途径的方法。有助于或破坏多基因表型的元件的连接多核苷酸片段在感兴趣的细胞中产生并表达。连接的多核苷酸片段被重组和/或突变以产生在感兴趣的细胞中表达的重组连接体的文库。联合多核苷酸片段和重组连接体的文库以附加方式表达或整合到细胞器或染色体的DNA中。筛选或选择细胞以鉴定呈现所需表型的细胞群体的成员。提供了包含组合多核苷酸片段和重组连接体的文库和载体，以及表达这些文库和载体或其组分的细胞。提供了含有联合多核苷酸片段，重组连接体，包含此类多核苷酸的载体和包含此类多核苷酸和载体的细胞的试剂盒。

10. 发明申请

WO0175087A3 SUBTILISIN VARIANTS 审中-公开
公开(公告)号：WO0175087A3
公开(公告)日：2002-12-19
申请号：PCT/US0110781
申请日：2001-04-02
申请人： MAXYGEN INC , NOVOZYMES AS , NESS JON E , WELCH MARK , GIVER LORRAINE J , CHERRY JOEL R , BORCHERT TORBEN V , STEMMER WILLEM P C , MINSHULL JEREMY
发明人： NESS JON E , WELCH MARK , GIVER LORRAINE J , CHERRY JOEL R , BORCHERT TORBEN V , STEMMER WILLEM P C , MINSHULL JEREMY
IPC分类号： C12N15/09 , C07K16/40 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/54 , C12N9/56 , C12N15/57 , G06F17/50
CPC分类号： C12N9/6408 , C12N9/54
摘要： New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.
摘要翻译：提供了新的枯草杆菌蛋白酶同源物（核酸和蛋白质）。还提供了包括这些新蛋白质，重组细胞，涉及新同系物的洗牌方法，新同系物抗体以及使用同源物的方法的组合物。

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