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1. 发明专利

JP2011217751A Optimization of crossover point for directed evolution 审中-公开
标题翻译：用于指导性演变的优化点优化
公开(公告)号：JP2011217751A
公开(公告)日：2011-11-04
申请号：JP2011112828
申请日：2011-05-19
申请人： Maxygen Inc , Mundorff Emily C , マキシジェン, インコーポレイテッド , マンドーフ, エミリーシー.
发明人： GOVINDARAJAN SRIDAR , GUSTAFSSON CLAES , MINSHULL JEREMY S
IPC分类号： C12N15/09 , G06N3/00 , C12N20060101 , C12Q1/68 , C40B50/02 , G01N33/48 , G01N33/50 , G01N33/53 , G06F17/00 , G06F17/10 , G06F17/11 , G06F19/16 , G06F19/22
CPC分类号： G06F19/22 , C40B50/02 , G06F19/16
摘要： PROBLEM TO BE SOLVED: To provide a method for producing libraries of biomolecules that are enriched for functional biomolecules.SOLUTION: Methods and devices for more efficiently engineering diversity into recombinant polypeptides and/or nucleic acids includes: for example, a variety of methods for selecting and/or assessing potential crossover sites in an amino acid sequence or a nucleotide sequence; and the resulting chimeric product sequences. The methods include, e.g. consideration of structural, functional and/or statistical data in the selection and assessment of sequences and crossover sites for use in recombination.
摘要翻译：待解决的问题：提供用于生产功能性生物分子富集的生物分子库的方法。解决方案：用于更有效地将多样性工程化成重组多肽和/或核酸的方法和装置包括：例如用于选择和/或评估氨基酸序列或核苷酸序列中的潜在交叉位点的多种方法; 和所得嵌合产物序列。所述方法包括例如考虑在重组中使用的序列和交换位点的选择和评估中的结构，功能和/或统计数据。版权所有（C）2012，JPO＆INPIT

2. 发明专利

JP2011040068A Method of populating data structure for use in evolutionary simulation 审中-公开
标题翻译：在演化模拟中使用数据结构的方法
公开(公告)号：JP2011040068A
公开(公告)日：2011-02-24
申请号：JP2010179767
申请日：2010-08-10
申请人： Maxygen Inc , マキシジェン, インコーポレイテッド
发明人： SLIFONOV SERGEY A , STEMMER WILLEM P C
IPC分类号： G06N3/00 , C12N15/09 , G06F19/00 , G06F19/14 , G06N3/12
摘要： PROBLEM TO BE SOLVED: To provide a method of populating data structures for use in evolutionary simulations. SOLUTION: There is provided novel methods of populating data structures for use in evolutionary simulations and provides methods of populating a data structure with a plurality of character strings. The methods involve: encoding two or more biological molecules into character strings to provide a collection of two or more different initial character strings; selecting at least two substrings from the pool of character strings; concatenating the substrings to form one or more product strings about the same length as one or more of the initial character strings; adding the product strings to a collection of strings; and optionally repeating this process using one or more of the product strings as an initial string in the collection of initial character strings. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供一种填充用于进化模拟的数据结构的方法。解决方案：提供了用于填充用于进化模拟的数据结构的新颖方法，并提供了使用多个字符串填充数据结构的方法。所述方法涉及：将两个或多个生物分子编码成字符串以提供两个或多个不同的初始字符串的集合; 从字符串池中选择至少两个子字符串; 连接子串以形成与一个或多个初始字符串相同长度的一个或多个产品串; 将产品字符串添加到字符串集合中; 并且可选地使用一个或多个产品串将该过程重复为初始字符串的集合中的初始字符串。版权所有（C）2011，JPO＆INPIT

3. 发明专利

JP2013010765A Pegylated g-csf polypeptide and method of producing the same 审中-公开
标题翻译： PEG聚G-CSF多肽及其制备方法
公开(公告)号：JP2013010765A
公开(公告)日：2013-01-17
申请号：JP2012173959
申请日：2012-08-06
申请人： Maxygen Inc , マキシジェン, インコーポレイテッド
发明人： GERMANSEN CARSTEN , SONI BOBBY , RASMUSSEN GRETHE
IPC分类号： C07K14/535 , A61K38/00 , A61P7/00 , C07K1/06 , C12P21/02
CPC分类号： C07K14/535 , A61K38/193 , A61K47/60 , C12P21/02
摘要： PROBLEM TO BE SOLVED: To provide a method for increasing the stability and uniformity of a PEGylated granulocyte colony stimulating factor (G-CSF).SOLUTION: The method for increasing the stability and uniformity of a PEGylated G-CSF polypeptide having at least one PEG moiety attached to the epsilon amino group of a lysine residue or the N-terminal amino group and at least one PEG moiety attached to a hydroxyl group, comprising subjecting the polypeptide to an elevated pH of above 8.0 for a period of time suitable to remove PEG moieties attached to a hydroxyl group, and reducing the pH to about 8.0 or lower.
摘要翻译：待解决的问题：提供增加聚乙二醇化粒细胞集落刺激因子（G-CSF）的稳定性和均匀性的方法。解决方案：增加具有连接于赖氨酸残基或N-末端氨基和至少一个PEG部分的ε氨基上的至少一个PEG部分的PEG化G-CSF多肽的稳定性和均匀性的方法包括使多肽进入高于8.0的升高的pH适合于除去连接到羟基上的PEG部分并将pH降低至约8.0或更低的时间。版权所有（C）2013，JPO＆INPIT

4. 发明专利

JP2010178757A Oligonucleotide-mediated nucleic acid recombination 审中-公开
标题翻译：寡核苷酸介导的核酸重组
公开(公告)号：JP2010178757A
公开(公告)日：2010-08-19
申请号：JP2010095500
申请日：2010-04-16
申请人： Maxygen Inc , マキシジェン, インコーポレイテッド
发明人： CRAMERI ANDREAS , STEMMER WILLEM P C , MINSHULL JEREMY , BASS STEVEN H , WELCH MARK , NESS JON E , GUSTAFSSON CLAES , PATTEN PHILIP A
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method for recombining nucleic acids including homologous nucleic acids, families of gene shuffling oligonucleotides, their use in a recombination procedure, and a polymerase- and ligase-mediated recombination method. SOLUTION: There is provided a method for oligonucleotide-assisted shuffling of nucleic acids. These oligonucleotide-assisted approaches especially facilitate a family shuffling procedure and provide an substantially simplified shuffling protocol. The protocol can be used for forming a family-shuffled nucleic acid without isolating or cloning whole-length homologous nucleic acids. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供重组核酸的方法，包括同源核酸，基因改组寡核苷酸家族，其在重组过程中的用途，以及聚合酶和连接酶介导的重组方法。解决方案：提供了核酸的寡核苷酸辅助改组方法。这些寡核苷酸辅助方法特别促进了家族洗牌过程并提供了基本上简化的洗牌方案。该方案可用于形成家族洗牌的核酸而不分离或克隆全长同源的核酸。版权所有（C）2010，JPO＆INPIT

5. 发明专利

JP2003174881A MUTAGENESIS OF DNA BY RANDOM FRAGMENTATION AND REASSEMBLY 审中-公开
公开(公告)号：JP2003174881A
公开(公告)日：2003-06-24
申请号：JP2002365621
申请日：2002-12-17
申请人： MAXYGEN INC
发明人： STEMMER WILLEM P C , CRAMERI ANDREAS
IPC分类号： C12N15/09 , A61K38/00 , A61K39/395 , C07K14/435 , C07K14/545 , C07K16/00 , C12N9/38 , C12N9/86 , C12N15/00 , C12N15/10 , C12P21/08 , C12Q1/68 , C12Q1/6811 , C40B40/02 , G01N33/53 , G01N33/566
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a mutant protein, and a method for developing a readily searchable large scale library of sequence of a mutant nucleic acid. SOLUTION: The method for reassembling DNA after random fragmentation and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination are described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The method of repeated cycles of mutagenesis, rearrangement and selection enables the in vitro or in vivo direct molecular evolution of proteins. COPYRIGHT: (C)2003,JPO

6. 发明专利

JP2001197895A METHOD AND COMPOSITION FOR CELL TECHNOLOGY AND METABOLISM TECHNOLOGY 失效
公开(公告)号：JP2001197895A
公开(公告)日：2001-07-24
申请号：JP2000379523
申请日：2000-12-13
申请人： MAXYGEN INC
发明人： MINSHULL JEREMY , STEMMER WILLEM P C
IPC分类号： C12N15/09 , C07K14/11 , C07K14/435 , C07K14/545 , C07K16/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/38 , C12N9/86 , C12N15/10 , C12N15/52 , C12N15/64 , C12Q1/02 , C12Q1/68 , C40B40/02
摘要： PROBLEM TO BE SOLVED: To provide a method for evolving a new metabolic pathway and increasing a bioprocess. SOLUTION: This method is method for evloving the new metabolic pathway comprising the process generally called circulative sequence recombination in this disclosure and increasing the bioprocess. The circulative sequence recombination necessitates to carry out the repetitive cycle of recombination and screening or selection to evolve individual gene, whole plasmid or virus, multigene cluster or even whole genome. The technics like this necessitates no wide range of analysis and calculation needed in the conventional method on the metabolism technology.

7. 发明专利

JP2000308490A PREPARATION OF POLYNUCLEOTIDE HAVING DESIRED CHARACTERISTICS BY REPEATED SELECTION AND RECOMBINATION 失效
公开(公告)号：JP2000308490A
公开(公告)日：2000-11-07
申请号：JP26784799
申请日：1999-09-21
申请人： MAXYGEN INC
发明人： STEMMER WILLEM P C , CRAMERI ANDREAS
IPC分类号： C12N15/09 , C07K14/435 , C07K14/46 , C07K14/545 , C07K16/00 , C12N9/38 , C12N9/86 , C12N15/10 , C12N15/52 , C12N15/64 , C12Q1/68 , C40B40/02 , H01L29/772 , H01L29/78 , H01L29/808 , H01L39/22
摘要： PROBLEM TO BE SOLVED: To provide a method for shuffling a nucleic acid by coding for nucleic acids for selecting variants of a nucleic acid, annealing these sequences in a similar region and heterologous region by multicyclic elongation, followed by selecting nucleic acids by an expression product. SOLUTION: This is a process of performing a multicyclic nucleic acid elongation process in a partially annealed nucleic acid strand having a sequence from the selected variants, wherein the nucleic acid codes for genes to select variants of a polynucleotide (nucleic acid) to be shuffled. A polynucleotide is shuffled by partially annealing via a similar region, followed by performing a method including a process of screening or selecting a recombinant nucleic acid coding for genes by a gene or an expression product under a condition where one strand acts as a template for elongation of other strand which is partially annealed so as to form a population of recombinant nucleic acid, wherein the nucleic acid strands has a similar region each other and a heterologous region.

8. 发明专利

JP2009089710A Method and composition for polypeptide engineering 审中-公开
标题翻译：多肽工程的方法与组合
公开(公告)号：JP2009089710A
公开(公告)日：2009-04-30
申请号：JP2008306897
申请日：2008-12-01
申请人： Maxygen Inc , マキシジェン, インコーポレイテッド
发明人： PATTEN PHILLIP A , STEMMER WILLEM P C
IPC分类号： C12N15/09 , A61K38/45 , A61K48/00 , C07K14/005 , C07K14/435 , C07K14/47 , C07K14/545 , C07K14/56 , C07K16/00 , C07K19/00 , C12N7/00 , C12N9/02 , C12N9/10 , C12N9/12 , C12N9/38 , C12N9/86 , C12N15/10 , C12N15/52 , C12N15/64 , C12P21/02 , C12Q1/02 , C12Q1/37 , C12Q1/68 , C40B40/02 , G01N33/68
CPC分类号： C12N15/1031 , A61K38/45 , A61K48/00 , C07K14/43595 , C07K14/545 , C07K14/56 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N7/00 , C12N9/0069 , C12N9/1007 , C12N9/16 , C12N9/86 , C12N15/10 , C12N15/1027 , C12N15/1034 , C12N15/1037 , C12N15/1058 , C12N15/52 , C12N15/64 , C12N15/67 , C12N2795/14043 , C12N2795/14143 , C12Q1/6811 , C12Q1/6853 , C12Q1/686 , C40B40/02 , C40B40/08 , C40B50/06 , G01N33/68 , G01N33/6845
摘要： PROBLEM TO BE SOLVED: To provide a number of strategies for evolving a polypeptide through a recursive recombination method. SOLUTION: A method for evolving a protein encoded by a DNA substrate molecule contains the following steps: (a) a step to digest at least the first and the second DNA substrate molecules with a restriction endonuclease, wherein the first and the second substrate molecules are different from each other at least by one nucleotide; (b) a step to connect the mixture for producing a library of recombinant DNA molecules; (c) a step to screen or select the product of the step (b) by a desired characteristic; and (d) a step to recover the recombinant DNA substrate molecule encoding the evolved protein. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供通过递归重组方法进化多肽的许多策略。解决方案：用于产生由DNA底物分子编码的蛋白质的方法包括以下步骤：（a）用限制性内切核酸酶消化至少第一和第二DNA底物分子的步骤，其中第一和第二底物分子至少一个核苷酸彼此不同; （b）将混合物连接以产生重组DNA分子文库的步骤; （c）按所需特性筛选或选择步骤（b）的产物的步骤; 和（d）回收编码进化蛋白质的重组DNA底物分子的步骤。版权所有（C）2009，JPO＆INPIT

9. 发明专利

JP2004254709A DNA MUTAGENESIS BY RANDOM FRAGMENTATION AND REASSEMBLY 审中-公开
公开(公告)号：JP2004254709A
公开(公告)日：2004-09-16
申请号：JP2004174720
申请日：2004-06-11
申请人： MAXYGEN INC
发明人： STEMMER WILLEM P C , CRAMERI ANDREAS
IPC分类号： C12N15/09 , A61K38/00 , A61K39/395 , C07K14/435 , C07K14/545 , C07K16/00 , C12N9/38 , C12N9/86 , C12N15/00 , C12N15/10 , C12P21/08 , C12Q1/68 , C12Q1/6811 , C40B40/02 , G01N33/53 , G01N33/566
摘要： PROBLEM TO BE SOLVED: To provide a method for generating a mutant protein and a developing method of a readily searchable large-scale library of a mutant nucleic acid sequence. SOLUTION: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequence by in vitro or in vivo recombination is described. More particularly, the method is used for generation of nucleic acid fragments or polynucleotides encoding mutant proteins. Moreover, the method provides repeated cycles of mutagenesis, shuffling and selection enabling the directed molecular evolution in vitro or in vivo of proteins. COPYRIGHT: (C)2004,JPO&NCIPI

10. 发明专利

JP2004187694A Methods for generating polynucleotides having desired characteristics by repetitive selection and recombination 审中-公开
公开(公告)号：JP2004187694A
公开(公告)日：2004-07-08
申请号：JP2004101193
申请日：2004-03-30
申请人： Maxygen Inc , マキシジェン, インコーポレイテッド
发明人： STEMMER WILLEM P C , CRAMERI ANDREAS
IPC分类号： C12N15/09 , C07K14/435 , C07K14/46 , C07K14/545 , C07K16/00 , C12N9/38 , C12N9/86 , C12N15/10 , C12N15/52 , C12N15/64 , C12Q1/68 , C40B40/02 , H01L29/772 , H01L29/78 , H01L29/808 , H01L39/22
CPC分类号： C40B40/02 , C07K14/43595 , C07K14/545 , C07K16/00 , C07K2317/565 , C07K2317/622 , C12N9/2471 , C12N9/86 , C12N15/1027 , C12N15/1034 , C12N15/1037 , C12N15/1058 , C12N15/52 , C12N15/64 , C12Q1/68 , C12Q1/6811 , C12Y302/01023 , C12Y305/02006 , H01L39/228 , C12Q2525/179
摘要： PROBLEM TO BE SOLVED: To provide a method for generating a mutant DNA, RNA or a large library of protein and selecting a specific mutant for a desired object. SOLUTION: In this method, a selected polynucleotide sequence or population of selected polynucleotide sequences is generated, typically in the form of amplified and/or cloned polynucleotides, the selected polynucleotide sequences possess a desired phenotypic characteristic (e.g., encode a polypeptide, promote transcription of linked polynucleotides, bind a protein, and the like) which can be selected for. COPYRIGHT: (C)2004,JPO&NCIPI

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