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1. 发明授权

US08008439B2 Biodegradable biopolymers, method for their preparation and functional materials constituted by these biopolymers 失效
公开(公告)号：US08008439B2
公开(公告)日：2011-08-30
申请号：US12076745
申请日：2008-03-21
申请人： Masuhiro Tsukada , Takayuki Arai
发明人： Masuhiro Tsukada , Takayuki Arai
IPC分类号： C07K14/435 , C08L89/04 , A61F13/00
CPC分类号： A61L27/3604 , A61K38/1767 , A61L27/227 , A61L27/48 , C12N5/0068 , C12N2533/50
摘要： A biodegradable biopolymer material consists of silk fibroin from domesticated silkworm; silk fibroin from wild silkworm; a composite material comprising silk fibroin from domesticated silkworm and silk fibroin from wild silkworm; or a composite material comprising either silk fibroin from domesticated silkworm or silk fibroin from wild silkworm and at least one secondary substance selected from the group consisting of cellulose, chitin, chitosan, chitosan derivatives, keratin from wool and polyvinyl alcohol. The material may be prepared by, for instance, casting an aqueous solution of domesticated silkworm silk fibroin on the surface of a substrate and then cast drying the applied solution. The biodegradable biopolymer material is effectively used as, for instance, a metal ion-adsorbing material, a sustained release substrate for a useful substance such as a medicine, a biological cell-growth substrate and a biodegradable water-absorbing material.

2. 发明申请

US20080131520A1 BIODEGRADABLE BIOPOLYMERS, METHOD FOR THEIR PREPARATION AND FUNCTIONAL MATERIALS CONSTITUTED BY THESE BIOPOLYMERS 审中-公开
公开(公告)号：US20080131520A1
公开(公告)日：2008-06-05
申请号：US12025484
申请日：2008-02-04
申请人： Masuhiro Tsukada , Takayuki Arai
发明人： Masuhiro Tsukada , Takayuki Arai
IPC分类号： A61K35/64
CPC分类号： A61L27/3604 , A61K38/1767 , A61L27/227 , A61L27/48 , C12N5/0068 , C12N2533/50
摘要： A biodegradable biopolymer material consists of silk fibroin from domesticated silkworm; silk fibroin from wild silkworm; a composite material comprising silk fibroin from domesticated silkworm and silk fibroin from wild silkworm; or a composite material comprising either silk fibroin from domesticated silkworm or silk fibroin from wild silkworm and at least one secondary substance selected from the group consisting of cellulose, chitin, chitosan, chitosan derivatives, keratin from wool and polyvinyl alcohol. The material may be prepared by, for instance, casting an aqueous solution of domesticated silkworm silk fibroin on the surface of a substrate and then cast drying the applied solution. The biodegradable biopolymer material is effectively used as, for instance, a metal ion-adsorbing material, a sustained release substrate for a useful substance such as a medicine, a biological cell-growth substrate and a biodegradable water-absorbing material.

3. 发明申请

US20080176960A1 Biodegradable biopolymers, method for their preparation and functional materials constituted by these biopolymers 失效
标题翻译：可生物降解的生物聚合物，其制备方法和由这些生物聚合物构成的功能材料
公开(公告)号：US20080176960A1
公开(公告)日：2008-07-24
申请号：US12076745
申请日：2008-03-21
申请人： Masuhiro Tsukada , Takayuki Arai
发明人： Masuhiro Tsukada , Takayuki Arai
IPC分类号： A61K47/42 , C07K14/435 , C12N5/00
CPC分类号： A61L27/3604 , A61K38/1767 , A61L27/227 , A61L27/48 , C12N5/0068 , C12N2533/50
摘要： A biodegradable biopolymer material consists of silk fibroin from domesticated silkworm; silk fibroin from wild silkworm; a composite material comprising silk fibroin from domesticated silkworm and silk fibroin from wild silkworm; or a composite material comprising either silk fibroin from domesticated silkworm or silk fibroin from wild silkworm and at least one secondary substance selected from the group consisting of cellulose, chitin, chitosan, chitosan derivatives, keratin from wool and polyvinyl alcohol. The material may be prepared by, for instance, casting an aqueous solution of domesticated silkworm silk fibroin on the surface of a substrate and then cast drying the applied solution. The biodegradable biopolymer material is effectively used as, for instance, a metal ion-adsorbing material, a sustained release substrate for a useful substance such as a medicine, a biological cell-growth substrate and a biodegradable water-absorbing material.
摘要翻译：可生物降解的生物聚合物材料由家蚕丝素蛋白组成; 来自野生蚕丝的丝素蛋白一种复合材料，包括来自家蚕的丝素蛋白和来自野生蚕的丝素蛋白; 或包含来自驯化的蚕丝的丝素蛋白或来自野蚕的丝素蛋白的复合材料和至少一种选自纤维素，壳多糖，壳聚糖，壳聚糖衍生物，来自羊毛和聚乙烯醇的角蛋白的次级物质。该材料可以通过例如将驯化的蚕丝素蛋白水溶液浇铸在基材的表面上，然后将所施加的溶液进行干燥来制备。可生物降解的生物聚合物材料有效地用作例如金属离子吸附材料，用于有用物质如药物，生物细胞生长基质和可生物降解的吸水材料的缓释基质。

4. 发明授权

US08048989B2 Biodegradable biopolymers, method for their preparation and functional materials constituted by these biopolymers 失效
标题翻译：可生物降解的生物聚合物，其制备方法和由这些生物聚合物构成的功能材料
公开(公告)号：US08048989B2
公开(公告)日：2011-11-01
申请号：US12878662
申请日：2010-09-09
申请人： Masuhiro Tsukada , Takayuki Arai
发明人： Masuhiro Tsukada , Takayuki Arai
IPC分类号： C07K14/435 , C08L89/04 , A61F13/00
CPC分类号： A61L27/3604 , A61K38/1767 , A61L27/227 , A61L27/48 , C12N5/0068 , C12N2533/50
摘要： A biodegradable biopolymer material consists of silk fibroin from domesticated silkworm; silk fibroin from wild silkworm; a composite material comprising silk fibroin from domesticated silkworm and silk fibroin from wild silkworm; or a composite material comprising either silk fibroin from domesticated silkworm or silk fibroin from wild silkworm and at least one secondary substance selected from the group consisting of cellulose, chitin, chitosan, chitosan derivatives, keratin from wool and polyvinyl alcohol. The material may be prepared by, for instance, casting an aqueous solution of domesticated silkworm silk fibroin on the surface of a substrate and then cast drying the applied solution. The biodegradable biopolymer material is effectively used as, for instance, a metal ion-adsorbing material, a sustained release substrate for a useful substance such as a medicine, a biological cell-growth substrate and a biodegradable water-absorbing material.
摘要翻译：可生物降解的生物聚合物材料由家蚕丝素蛋白组成; 来自野生蚕丝的丝素蛋白一种复合材料，包括来自家蚕的丝素蛋白和来自野生蚕的丝素蛋白; 或包含来自驯化的蚕丝的丝素蛋白或来自野蚕的丝素蛋白的复合材料和至少一种选自纤维素，壳多糖，壳聚糖，壳聚糖衍生物，来自羊毛和聚乙烯醇的角蛋白的次级物质。该材料可以通过例如将驯化的蚕丝素蛋白水溶液浇铸在基材的表面上，然后将所施加的溶液进行干燥来制备。可生物降解的生物聚合物材料有效地用作例如金属离子吸附材料，用于有用物质如药物，生物细胞生长基质和可生物降解的吸水材料的缓释基质。

5. 发明申请

US20080124473A1 BIODEGRADABLE BIOPOLYMERS, METHOD FOR THEIR PREPARATION AND FUNCTIONAL MATERIALS CONSTITUTED BY THESE BIOPOLYMERS 审中-公开
标题翻译：生物可降解生物聚合物，由这些生物反应器构成的其制备和功能材料的方法
公开(公告)号：US20080124473A1
公开(公告)日：2008-05-29
申请号：US12025524
申请日：2008-02-04
申请人： Masuhiro Tsukada , Takayuki Arai
发明人： Masuhiro Tsukada , Takayuki Arai
IPC分类号： B05D3/02
CPC分类号： A61L27/3604 , A61K38/1767 , A61L27/227 , A61L27/48 , C12N5/0068 , C12N2533/50
摘要： A biodegradable biopolymer material consists of silk fibroin from domesticated silkworm; silk fibroin from wild silkworm; a composite material comprising silk fibroin from domesticated silkworm and silk fibroin from wild silkworm; or a composite material comprising either silk fibroin from domesticated silkworm or silk fibroin from wild silkworm and at least one secondary substance selected from the group consisting of cellulose, chitin, chitosan, chitosan derivatives, keratin from wool and polyvinyl alcohol. The material may be prepared by, for instance, casting an aqueous solution of domesticated silkworm silk fibroin on the surface of a substrate and then cast drying the applied solution. The biodegradable biopolymer material is effectively used as, for instance, a metal ion-adsorbing material, a sustained release substrate for a useful substance such as a medicine, a biological cell-growth substrate and a biodegradable water-absorbing material.
摘要翻译：可生物降解的生物聚合物材料由家蚕丝素蛋白组成; 来自野生蚕丝的丝素蛋白一种复合材料，包括来自家蚕的丝素蛋白和来自野生蚕的丝素蛋白; 或包含来自驯化的蚕丝的丝素蛋白或来自野蚕的丝素蛋白的复合材料和至少一种选自纤维素，壳多糖，壳聚糖，壳聚糖衍生物，来自羊毛和聚乙烯醇的角蛋白的次级物质。该材料可以通过例如将驯化的蚕丝素蛋白水溶液浇铸在基材的表面上，然后将所施加的溶液进行干燥来制备。可生物降解的生物聚合物材料有效地用作例如金属离子吸附材料，用于有用物质如药物，生物细胞生长基质和可生物降解的吸水材料的缓释基质。

6. 发明授权

US6156330A Chitin beads, chitosan beads, process for preparing these beads, carrier comprising said beads, and process for preparing microsporidian spore 失效
标题翻译：甲壳素珠，壳聚糖珠，制备这些珠的方法，包含所述珠的载体和用于制备微孢子虫孢子的方法
公开(公告)号：US6156330A
公开(公告)日：2000-12-05
申请号：US214383
申请日：1998-12-30
申请人： Masuhiro Tsukada , Akira Shirata , Sho Ji Hayasaka
发明人： Masuhiro Tsukada , Akira Shirata , Sho Ji Hayasaka
IPC分类号： C08B37/08 , A01N25/10 , A01N25/26 , A23K1/00 , A23K1/16 , A23L1/056 , A23L1/22 , A61K9/16 , C09B67/02 , A01N25/28
CPC分类号： C09B67/0097 , A01N25/10 , A01N25/26 , A23K20/163 , A23K40/30 , A23L27/72 , A23L29/275 , A61K9/1652
摘要： The present invention relates to chitin beads having a uniform and fine particle size and comprising microsporidian spores whose principal cell wall substance is chitin; chitosan beads comprising N-deacetylated chitin and having a uniform fine particle size; methods for preparing these beads; carriers comprising these beads; and a method for preparing microsporidian spores.Microsporidian spores are proliferated in insect's bodies or cultured cells to obtain chitin beads having the uniform and fine particle size and whose cell wall substance is mainly composed of chitin. The chitin as the major cell wall substance is subjected to N-deacetylation to give chitosan beads. When the insect is a larva of domesticated silkworm, microsporidian spores are proliferated by orally or percutaneously inoculating the spores into the 2nd instar domesticated silkworm larvae in a concentration of 5.times.10.sup.2 to 5.times.10.sup.8 spores/ml, then the proliferated microsporidian spores are isolated from the bodies of the grown 5th instar domesticated silkworm larvae, followed by purifying the resulting spores to give chitin beads having the uniform and fine particle size and chitosan beads can be obtained from the chitin beads in the same manner used above. These beads are used as carriers for the immobilization or encapsulation of a variety of substances such as an antibiotic and a microorganism.
摘要翻译： PCT No.PCT / JP97 / 03741 Sec。 371日期1998年12月30日第 102（e）日期1998年12月30日PCT 1997年10月16日PCT公布。公开号WO98 / 51711 PCT 日期：1998年11月19日本发明涉及具有均匀且细粒度的几丁质珠粒，其包含主要细胞壁物质为壳多糖的微孢子虫孢子; 包含N-脱乙酰化几丁质并具有均匀细粒度的壳聚糖珠粒; 制备这些珠粒的方法; 包含这些珠的载体; 和一种制备微孢子虫孢子的方法。微孢子虫孢子在昆虫体内或培养细胞中增殖，获得具有均匀和细粒度的壳多糖颗粒，其细胞壁物质主要由甲壳质组成。作为主要细胞壁物质的几丁质进行N-脱乙酰化，得到壳聚糖珠。当昆虫是家蚕的幼虫时，微孢子虫孢子通过口服或经皮接种孢子以5×102-5×10 8孢子/ ml浓度的2龄家蚕幼虫进行增殖，然后将增殖的微孢子虫孢子从生长的五龄驯化的蚕幼虫，然后纯化所得的孢子，得到具有均匀和细粒度的壳多糖珠，并且可以以与上述相同的方式从壳多糖珠获得壳聚糖珠。这些珠被用作固定或包封各种物质如抗生素和微生物的载体。

7. 发明授权

US06894153B1 Gene Any-RF; dormancy regulatory substance, process for producing the same and cell regulator for vital cells 失效
标题翻译：基因Any-RF; 休眠调节物质，生产细胞的过程和细胞调节剂
公开(公告)号：US06894153B1
公开(公告)日：2005-05-17
申请号：US09674436
申请日：2000-05-26
申请人： Toyomi Kotaki , Masuhiro Tsukada , Koichi Suzuki , Ping Yang
发明人： Toyomi Kotaki , Masuhiro Tsukada , Koichi Suzuki , Ping Yang
IPC分类号： A61K38/00 , A61P35/00 , C07K14/435 , C12N15/12 , A61K38/02 , C07K1/16 , C12P21/02
CPC分类号： C07K14/43563 , A61K38/00
摘要： The present invention provides a gene Any-RF derived from an insect, having dormancy-control activity and biological cell-control function; a dormancy-control substance and a method for preparing the same; as well as a biological cell-control agent, which comprises, as an effective component, a physiologically active substance having a biological cell-control function and which never causes any antigen-antibody reaction in the living body.The gene encodes for a protein having an amino acid sequence specified as Sequence No. 1 in SEQUENCE LISTING: Asp-Ile-Leu-Arg-Gly, whose C-terminal is amidated and having a molecular weight of 570.959. The physiologically active substance comprising such a gene is a peptide, which can be isolated and purified by adding an acid-methanol aqueous solution to Antheraea yamamai, triturating the resulting mixture, centrifuging the same and then treating the resulting supernatant in an HPLC system. The peptide permits the control of the dormancy of insects and is also useful as a biological cell-control agent, which comprises, as an effective component, such a peptide.
摘要翻译：本发明提供了具有休眠控制活性和生物细胞控制功能的来自昆虫的基因Any-RF; 休眠控制物质及其制备方法。以及生物细胞控制剂，其包含作为有效成分的具有生物细胞控制功能的生物活性物质，并且在生物体中不会引起任何抗原 - 抗体反应。基因编码具有氨基酸序列的序列SEQ ID NO：I-Leu-Leu-Arg-Gly序列号1的氨基酸序列的氨基酸序列，其C-末端酰胺化，分子量为570.959。包含这种基因的生理活性物质是一种肽，其可以通过向Antheraea yamamai加入酸 - 甲醇水溶液来分离和纯化，研磨所得混合物，将其离心，然后在HPLC系统中处理所得上清液。该肽允许控制昆虫的休眠，并且还可用作生物细胞控制剂，其包含作为有效成分的这种肽。

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