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1. 发明授权

US5272065A Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA 失效
标题翻译：通过使用干扰互补MRNA的MRNA的翻译抑制来调节基因表达
公开(公告)号：US5272065A
公开(公告)日：1993-12-21
申请号：US542209
申请日：1990-06-21
申请人： Masayori Inouye , Takeshi Mizuno , Mei-Yin Chou
发明人： Masayori Inouye , Takeshi Mizuno , Mei-Yin Chou
IPC分类号： A61K38/00 , C07H21/00 , C12N15/113 , C12P19/34 , A61K48/00 , C07H15/12
CPC分类号： C12N15/113 , C07H21/00 , A61K38/00 , C12N2310/111
摘要： Gene expression in a cell can be regulated or inhibited by incorporating into or associating with the genetic material of the cell a non-native nucleic acid sequence which is transcribed to produce an mRNA which is complementary to and capable of binding to the mRNA produced by the genetic material of said cell.
摘要翻译：细胞中的基因表达可通过将细胞的遗传物质并入或与细胞的遗传物质相关联而被调节或抑制，所述非天然核酸序列被转录以产生与mRNA产生的mRNA互补并能够结合mRNA 所述细胞的遗传物质。

2. 发明授权

US08476037B2 Method for screening of antiviral agents 有权
标题翻译：筛选抗病毒剂的方法
公开(公告)号：US08476037B2
公开(公告)日：2013-07-02
申请号：US13213230
申请日：2011-08-19
申请人： Masayori Inouye , Lili Mao , William F. Degrado , Nathan H. Joh
发明人： Masayori Inouye , Lili Mao , William F. Degrado , Nathan H. Joh
IPC分类号： C12Q1/02
CPC分类号： C12Q1/18
摘要： A method for screening for an antiviral agent capable of blocking a viral viroporin by determining whether a test agent can rescue expression of a fragment of a viral viroporin in a Single Protein Production system of Escherichia coli is provided.
摘要翻译：提供了通过确定测试试剂是否可以在大肠杆菌的单一蛋白质生产系统中拯救病毒性异丙肾上腺素的片段的表达来筛选能够阻断病毒性丙泊酚的抗病毒剂的方法。

3. 发明申请

US20130071374A1 RNA Interferases and Methods of Use Thereof 审中-公开
标题翻译： RNA干扰物及其使用方法
公开(公告)号：US20130071374A1
公开(公告)日：2013-03-21
申请号：US13449095
申请日：2012-04-17
申请人： Masayori Inouye , Junjie Zhang , Yong Long Zhang
发明人： Masayori Inouye , Junjie Zhang , Yong Long Zhang
IPC分类号： C12N9/22 , C12P19/34 , A61K38/46 , C12Q1/44
CPC分类号： C12N9/22 , A61K38/465 , C12N15/70 , C12P19/34 , C12Q1/42 , C12Q1/44 , G01N2333/922 , G01N2800/26
摘要： The present invention is directed to the discovery of a novel family of enzymes designated herein as mRNA interferases that exhibit endoribonuclease activity. The novel finding of the present inventors, therefore, presents new applications for which mRNA interferase nucleic and amino acid sequences, and compositions thereof may be used to advantage. The invention also encompasses screening methods to identify compounds/agents capable of modulating mRNA interferase activity and methods for using such compounds/agents. Also provided is a kit comprising mRNA interferase nucleic and/or amino acid sequences, mRNA interferase activity compatible buffers, and instruction materials.
摘要翻译：本发明涉及在本文中称为表达内切核糖核酸酶活性的mRNA干扰酶的新型家族的发现。因此，本发明人的新发现提出了可以使用mRNA干扰酶核酸和氨基酸序列及其组合物的新用途。本发明还包括鉴定能够调节mRNA干扰酶活性的化合物/试剂的筛选方法以及使用这些化合物/试剂的方法。还提供了包含mRNA干扰酶核酸和/或氨基酸序列，mRNA干扰酶活性相容缓冲液和说明材料的试剂盒。

4. 发明申请

US20130029426A1 Dual Inducible System for the cSPP System 有权
标题翻译： cSPP系统的双诱导系统
公开(公告)号：US20130029426A1
公开(公告)日：2013-01-31
申请号：US13556100
申请日：2012-07-23
申请人： Masayori Inouye , Lili Mao , S. Thangminlal Vaiphei , Yojiro Ishida
发明人： Masayori Inouye , Lili Mao , S. Thangminlal Vaiphei , Yojiro Ishida
IPC分类号： C12N1/21 , C12N15/70
CPC分类号： C12N15/70 , C12P21/02
摘要： The present invention provides a dual inducible system for single protein production, as well as a method of inducing high level protein expression using amino acids.
摘要翻译：本发明提供了用于单蛋白生产的双诱导系统，以及使用氨基酸诱导高水平蛋白表达的方法。

5. 发明申请

US20100035346A1 SINGLE PROTEIN PRODUCTION IN LIVING CELLS FACILITATED BY A MESSENGER RNA INTERFERASE 有权
标题翻译：生物细胞中的单蛋白生产由信使RNA干扰素
公开(公告)号：US20100035346A1
公开(公告)日：2010-02-11
申请号：US11750314
申请日：2007-05-17
申请人： Masayori Inouye , Junjie Zhang , Motoo Suzuki
发明人： Masayori Inouye , Junjie Zhang , Motoo Suzuki
IPC分类号： C12N15/74 , C12N1/21
CPC分类号： C12N9/22 , C12N15/67 , C12N15/74
摘要： The present invention describes a single-protein production (SPP) system in living E. coli cells that exploits the unique properties of an mRNA interferase, for example, MazF, a bacterial toxin that is a single stranded RNA- and ACA-specific endoribonuclease, which efficiently and selectively degrades all cellular mRNAs in vivo, resulting in a precipitous drop in total protein synthesis. Concomitant expression of MazF and a target gene engineered to encode an ACA-less mRNA results in sustained and high-level (up to 90%) target expression in the virtual absence of background cellular protein synthesis. Remarkably, target synthesis continues for at least 4 days, indicating that cells retain transcriptional and translational competence despite their growth arrest. SPP technology works well for yeast and human proteins, even a bacterial integral membrane protein. This novel system enables unparalleled signal to noise ratios that should dramatically simplify structural and functional studies of previously intractable but biologically important proteins. The present invention also provides an optimized condensed single protein production system.
摘要翻译：本发明描述了利用mRNA干扰酶的独特性质的活的大肠杆菌细胞中的单蛋白质生产（SPP）系统，例如MazF，单链RNA-和ACA特异性内切核糖核酸酶的细菌毒素，其有效和选择性地降解体内所有细胞mRNA，导致总蛋白质合成的急剧下降。 MazF和旨在编码无ACA mRNA的靶基因的伴随表达导致在虚拟无背景细胞蛋白质合成中的持续和高水平（高达90％）的靶表达。值得注意的是，目标合成持续至少4天，表明细胞保留转录和翻译能力，尽管其生长停滞。 SPP技术适用于酵母和人类蛋白质，甚至细菌整合膜蛋白。这种新颖的系统能够实现无与伦比的信噪比，这将显着简化以前难以处理但生物重要的蛋白质的结构和功能研究。本发明还提供优化的冷凝单蛋白生产系统。

6. 发明申请

US20050116263A1 MULTIFUNCTIONAL BIOSENSOR BASED ON ZNO NANOSTRUCTURES 有权
标题翻译：基于ZNO纳米结构的多功能生物传感器
公开(公告)号：US20050116263A1
公开(公告)日：2005-06-02
申请号：US10456050
申请日：2003-06-06
申请人： Yicheng Lu , Zheng Zhang , Nuri Emanetoglu , Masayori Inouye , Oleg Mirochnitchenko
发明人： Yicheng Lu , Zheng Zhang , Nuri Emanetoglu , Masayori Inouye , Oleg Mirochnitchenko
IPC分类号： C12Q1/68 , G01N21/64 , G01N27/00 , G01N27/12 , G01N27/414 , G01N29/02 , G01N33/543 , H01L29/84
CPC分类号： G01N21/6428 , C12Q1/6825 , C12Q1/6837 , G01N27/4145 , G01N27/4146 , G01N29/022 , G01N33/54373 , G01N33/5438 , G01N2021/6432 , G01N2291/011 , G01N2291/014 , G01N2291/0255 , G01N2291/0256 , G01N2291/0423 , G01N2291/0426 , C12Q2565/501 , C12Q2565/607
摘要： The present invention provides the multifunctional biological and biochemical sensor technology based on ZnO nanostructures. The ZnO nanotips serve as strong DNA or protein molecule binding sites to enhance the immobilization. Patterned ZnO nanotips are used to provide conductivity-based biosensors. Patterned ZnO nanotips are also used as the gate for field-effect transistor (FET) type sensors. Patterned ZnO nanotips are integrated with SAW or BAW based biosensors. These ZnO nanotip based devices operate in multimodal operation combining electrical, acoustic and optical sensing mechanisms. The multifunctional biosensors can be arrayed and combined into one biochip, which will enhance the sensitivity and accuracy of biological and biochemical detection due to strong immobilization and multimodal operation capability. Such biological and biochemical sensor technology are useful in detection of RNA-DNA, DNA-DNA, protein-protein, protein-DNA and protein-small molecules interaction. It can be further applied for drug discovery, and for environmental monitoring and protection.
摘要翻译：本发明提供了基于ZnO纳米结构的多功能生物和生物化学传感器技术。 ZnO纳米尖端作为强的DNA或蛋白质分子结合位点来增强固定。图案化的ZnO纳米尖端用于提供基于导电性的生物传感器。图案化的ZnO纳米技术也用作场效应晶体管（FET）型传感器的栅极。图案化的ZnO纳米片与SAW或基于BAW的生物传感器集成。这些基于ZnO纳米管的器件在电气，声学和光学感测机构的多模式操作中工作。多功能生物传感器可以排列并组合成一个生物芯片，由于强固定和多模态操作能力，提高了生物和生化检测的灵敏度和准确性。这种生物和生化传感器技术可用于RNA-DNA，DNA-DNA，蛋白质 - 蛋白质，蛋白质 - DNA和蛋白质 - 小分子相互作用的检测。可进一步应用于药物发现，环境监测和保护。

7. 发明授权

US6043028A Method for synthesizing single-stranded stem-loop DNA's the products and uses therefore 失效
标题翻译：单链茎环DNA的合成方法及其用途
公开(公告)号：US6043028A
公开(公告)日：2000-03-28
申请号：US430277
申请日：1995-04-28
申请人： Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
发明人： Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
IPC分类号： C12N15/09 , C07H21/04 , C12N1/19 , C12N1/21 , C12N9/38 , C12N9/86 , C12N15/10 , C12N15/113 , C12N15/70 , C12N15/81 , C12Q1/68 , C12R1/19 , C12R1/865 , C12N15/64 , C12P19/34
CPC分类号： C12N15/113 , C12N15/10 , C12N15/70 , C12N15/81 , C12N9/2471 , C12N9/86 , C12Q1/6853 , C12Q1/6867 , C12Y302/01023 , C12N2310/111 , C12N2310/15 , C12N2310/53
摘要： A method of synthesis of new and useful single-stranded DNAs which have a stem-loop configuration (ss-slDNA). The method is an in vivo or an in vitro synthesis. Replicating vehicles which produce these ss-slDNAs. The ss-slDNAs are described. Uses for these slDNAs are disclosed. They can be used for introducing random mutations, they lend themselves for replication by a variant of the PCR method. They can also be used for regulating gene function. Other uses are disclosed.
摘要翻译：合成具有茎 - 环构型（ss-slDNA）的新的和有用的单链DNA的方法。该方法是体内或体外合成。复制产生这些ss-SLDNA的载体。描述了ss-SLDNA。公开了这些用于这些slDNA的用途。它们可用于引入随机突变，它们通过PCR方法的变体使其复制。它们也可用于调节基因功能。披露了其他用途。

8. 发明授权

US5981280A Method and constructs for inhibiting protein expression in bacteria 失效
标题翻译：用于抑制细菌中蛋白质表达的方法和构建体
公开(公告)号：US5981280A
公开(公告)日：1999-11-09
申请号：US769945
申请日：1996-12-19
申请人： Li Fang , Weining Jiang , Masanori Mitta , Masayori Inouye
发明人： Li Fang , Weining Jiang , Masanori Mitta , Masayori Inouye
IPC分类号： C12N15/09 , C12N1/21 , C12N15/113 , C12N15/63 , C12N15/67 , C12P21/02 , C12Q1/02 , C07H21/02 , C07H21/04
CPC分类号： C12N15/67 , C12N15/113 , C12N15/63 , C12N2310/111
摘要： A method of inhibiting the translation of bacterial mRNA is disclosed, which method comprises overexpressing in a bacterium an mRNA which contains a sequence which is complementary to the anti-downstream box region of the 16S rRNA. RNA and DNA constructs for the overexpression of the mRNA of the invention are disclosed.
摘要翻译：公开了一种抑制细菌mRNA翻译的方法，该方法包括在细菌中过表达含有与16S rRNA的反向下游盒区互补的序列的mRNA。公开了用于本发明的mRNA的过表达的RNA和DNA构建体。

9. 发明授权

US4643969A Novel cloning vehicles for polypeptide expression in microbial hosts 失效
标题翻译：用于微生物宿主中多肽表达的新型克隆载体
公开(公告)号：US4643969A
公开(公告)日：1987-02-17
申请号：US494040
申请日：1983-07-25
申请人： Masayori Inouye , Yoshihiro Masui
发明人： Masayori Inouye , Yoshihiro Masui
IPC分类号： C12N15/62 , C12N15/70 , C12P21/00 , C12N1/00 , C12N15/00
CPC分类号： C12N15/62 , C12N15/70 , Y10S930/30
摘要： Methods and compositions are provided for regulated expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide (on an insertion site therefor) linked for transcriptional expression in reading phase with four functional fragments derived from the lipoprotein gene of E. coli. The plasmids further include a DNA sequence coding for a specific segment of the E. coli lac promoter-operator, which is positioned in the proper orientation for transcriptional expression of the desired polypeptide, as well as a separate functional E. coli lacI gene coding for the associated repressor molecule which can interact with the lac promoter-operator to prevent transcription therefrom. Expression of the desired polypeptide is under the control of both the constitutive promoter and the inducible promoter, although transcription from either promotor is normally blocked by the repressor molecule. However, the repressor can be selectively inactivated by means of an inducer molecule to permit transcriptional expression of the desired polypeptide from both promoter. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of proteins, such as medically or commerically useful hormones, enzymes, immunogenic proteins, or intermediates therefor, but only in the presence of an appropriate inducer.
摘要翻译：提供了用于在转化的细菌宿主中调节多肽表达的方法和组合物。一类新颖的质粒克隆载体包括编码与在大肠杆菌的脂蛋白基因中衍生的四个功能片段相连的用于在阅读阶段转录表达的期望多肽（在其插入位点上）的DNA序列。质粒还包括编码大肠杆菌lac启动子 - 操纵子的特定区段的DNA序列，其定位于所需多肽的转录表达的正确取向，以及编码所述多肽的单独的功能性大肠杆菌lacI基因可与lac启动子 - 操纵子相互作用以阻止转录的相关阻遏物分子。所需多肽的表达受组成型启动子和诱导型启动子的控制，尽管来自启动子的转录通常被阻遏物分子阻断。然而，阻遏物可以通过诱导剂分子选择性地失活以允许来自两个启动子的所需多肽的转录表达。所述方法利用这种质粒将遗传能力引入微生物以产生蛋白质，例如医学或商业上有用的激素，酶，免疫原性蛋白质或其中间体，但仅在适当的诱导剂存在下。

10. 发明授权

US4624926A Novel cloning vehicles for polypeptide expression in microbial hosts 失效
标题翻译：用于微生物宿主中多肽表达的新型克隆载体
公开(公告)号：US4624926A
公开(公告)日：1986-11-25
申请号：US354287
申请日：1982-03-03
申请人： Masayori Inouye , Kenzo Nakamura
发明人： Masayori Inouye , Kenzo Nakamura
IPC分类号： C07K14/62 , C12N15/62 , C12N1/20 , C12N1/00 , C12N15/00
CPC分类号： C07K14/62 , C12N15/62 , C07K2319/02 , Y10S930/30
摘要： Methods and compositions are provided for expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide linked in reading phase with one or more functional fragments derived from an outer membrane protein gene of a gramnegative bacterium. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor.
摘要翻译：提供了用于在转化的细菌宿主中表达多肽的方法和组合物。一类新颖的质粒克隆载体包括编码在阅读阶段中连接的期望多肽的DNA序列与一种或多种衍生自革兰氏动物细菌的外膜蛋白基因的功能片段。该方法利用这种质粒将遗传能力引入微生物以产生蛋白质，例如医学上或商业上有用的激素，酶，免疫原性蛋白质或其中间体。

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