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1. 发明授权

US5981280A Method and constructs for inhibiting protein expression in bacteria 失效
标题翻译：用于抑制细菌中蛋白质表达的方法和构建体
公开(公告)号：US5981280A
公开(公告)日：1999-11-09
申请号：US769945
申请日：1996-12-19
申请人： Li Fang , Weining Jiang , Masanori Mitta , Masayori Inouye
发明人： Li Fang , Weining Jiang , Masanori Mitta , Masayori Inouye
IPC分类号： C12N15/09 , C12N1/21 , C12N15/113 , C12N15/63 , C12N15/67 , C12P21/02 , C12Q1/02 , C07H21/02 , C07H21/04
CPC分类号： C12N15/67 , C12N15/113 , C12N15/63 , C12N2310/111
摘要： A method of inhibiting the translation of bacterial mRNA is disclosed, which method comprises overexpressing in a bacterium an mRNA which contains a sequence which is complementary to the anti-downstream box region of the 16S rRNA. RNA and DNA constructs for the overexpression of the mRNA of the invention are disclosed.
摘要翻译：公开了一种抑制细菌mRNA翻译的方法，该方法包括在细菌中过表达含有与16S rRNA的反向下游盒区互补的序列的mRNA。公开了用于本发明的mRNA的过表达的RNA和DNA构建体。

2. 发明授权

US07429654B1 Cold-shock regulatory elements, constructs thereof, and method of use 有权
标题翻译：冷休克调节元件，其构建体和使用方法
公开(公告)号：US07429654B1
公开(公告)日：2008-09-30
申请号：US09552272
申请日：2000-04-19
申请人： Li Fang , Weining Jiang , Masanori Mitta , Masayori Inouye , Jean-Pierre Etchegaray , Kunitoshi Yamanaka
发明人： Li Fang , Weining Jiang , Masanori Mitta , Masayori Inouye , Jean-Pierre Etchegaray , Kunitoshi Yamanaka
IPC分类号： C07H21/04
CPC分类号： C12N15/67 , C12N15/113 , C12N15/63 , C12N2310/111
摘要： Regulatory elements of the 5′UTR of cold-shock inducible genes that prolong the expression of cold-shock inducible genes under conditions that elicit the cold-shock response in a bacterium, repress the expression of the cold-shock inducible genes under physiological conditions, or enhance the translation of cold-shock inducible genes under conditions that elicit a cold-shock response in bacteria; vectors incorporating these elements; and methods for expressing proteins.
摘要翻译：冷激诱导型基因的5'UTR的调控元件在细菌诱导冷休克反应的条件下延长冷休克诱导型基因的表达，抑制生理条件下的冷休克诱导型基因的表达，或在细菌引起冷休克反应的条件下增强冷休克诱导型基因的翻译; 包含这些元素的载体; 和表达蛋白质的方法。

3. 发明授权

US06686174B1 Method and constructs for inhibiting protein expression in bacteria 有权
标题翻译：用于抑制细菌中蛋白质表达的方法和构建体
公开(公告)号：US06686174B1
公开(公告)日：2004-02-03
申请号：US09293427
申请日：1999-04-16
申请人： Li Fang , Weinning Jiang , Masanori Mitta , Masayori Inouye
发明人： Li Fang , Weinning Jiang , Masanori Mitta , Masayori Inouye
IPC分类号： C07H2102
CPC分类号： C12N15/67 , C12N15/113 , C12N15/63 , C12N2310/111
摘要： A method of inhibiting the translation of bacterial mRNA is disclosed. The method comprises overexpressing in a bacterium an mRNA which contains a sequence which is complementary to the anti-downstream box region of the 16S rRNA. RNA and DNA constructs for the overexpression of the mRNA of the invention are disclosed. Further, there are disclosed isolated DNA constructs that direct the prolonged expression of a heterologous gene in a cold-shocked bacterium at reduced temperature. The construct can comprise a promoter region of a cold-shocked inducible gene. The replication vehicle comprising such DNA constructs and a method for overexpressing a heterologous gene in a bacterium transformed with such a replication vehicle are also disclosed.
摘要翻译：公开了抑制细菌mRNA翻译的方法。该方法包括在细菌中过表达含有与16S rRNA的反向下游盒区域互补的序列的mRNA。公开了用于本发明的mRNA的过表达的RNA和DNA构建体。此外，公开了分离的DNA构建体，其在降温的温度下将异源基因的延长表达指导在冷的细胞中。构建体可以包含冷激诱导型基因的启动子区。还公开了包含这种DNA构建体的复制载体和用这种复制载体转化的细菌中过表达异源基因的方法。

4. 发明授权

US06333191B1 Nucleic acids sequence, stress-induced proteins and uses thereof 失效
标题翻译：核酸序列，应激诱导的蛋白质及其用途
公开(公告)号：US06333191B1
公开(公告)日：2001-12-25
申请号：US09017754
申请日：1998-02-03
申请人： Masayori Inouye , Pamela Jones , Jean-Pierre Etchegaray , Weining Jiang , N. Stephen Pollitt , Joel Goldstein
发明人： Masayori Inouye , Pamela Jones , Jean-Pierre Etchegaray , Weining Jiang , N. Stephen Pollitt , Joel Goldstein
IPC分类号： C12N1500
CPC分类号： C12N15/70 , C07K14/245 , C12N15/8273
摘要： A family of stimuli-induced in particular stress or cold-shock induced genes and proteins are disclosed which have conserved amino acid domains. Nucleic acid sequences of the genes and the promoters are also described. Various utilities of the promoters and of the proteins are disclosed.
摘要翻译：公开了一种具有特定应激或冷休克诱导的基因和蛋白质的刺激物，其具有保守的氨基酸结构域。还描述了基因和启动子的核酸序列。公开了启动子和蛋白质的各种效用。

5. 发明授权

US5714575A Nucleic acids sequence, stress-induced proteins and uses thereof 失效
标题翻译：核酸序列，应激诱导的蛋白质及其用途
公开(公告)号：US5714575A
公开(公告)日：1998-02-03
申请号：US203806
申请日：1994-03-01
申请人： Masayori Inouye , Pamela Jones , Jean-Pierre Etchegaray , Weining Jiang , N. Stephen Pollitt , Joel Goldstein
发明人： Masayori Inouye , Pamela Jones , Jean-Pierre Etchegaray , Weining Jiang , N. Stephen Pollitt , Joel Goldstein
IPC分类号： C07K14/245 , C12N15/70 , C12N15/82 , C07K14/24
CPC分类号： C12N15/70 , C07K14/245 , C12N15/8273
摘要： A family of stimuli-induced in particular stress or cold-shock induced genes and proteins are disclosed which have conserved amino acid domains. Nucleic acid sequences of the genes and the promoters are also described. Various utilities of the promoters and of the proteins are disclosed.
摘要翻译：公开了一种具有特定应激或冷休克诱导的基因和蛋白质的刺激物，其具有保守的氨基酸结构域。还描述了基因和启动子的核酸序列。公开了启动子和蛋白质的各种效用。

6. 发明授权

US08476037B2 Method for screening of antiviral agents 有权
标题翻译：筛选抗病毒剂的方法
公开(公告)号：US08476037B2
公开(公告)日：2013-07-02
申请号：US13213230
申请日：2011-08-19
申请人： Masayori Inouye , Lili Mao , William F. Degrado , Nathan H. Joh
发明人： Masayori Inouye , Lili Mao , William F. Degrado , Nathan H. Joh
IPC分类号： C12Q1/02
CPC分类号： C12Q1/18
摘要： A method for screening for an antiviral agent capable of blocking a viral viroporin by determining whether a test agent can rescue expression of a fragment of a viral viroporin in a Single Protein Production system of Escherichia coli is provided.
摘要翻译：提供了通过确定测试试剂是否可以在大肠杆菌的单一蛋白质生产系统中拯救病毒性异丙肾上腺素的片段的表达来筛选能够阻断病毒性丙泊酚的抗病毒剂的方法。

7. 发明申请

US20130071374A1 RNA Interferases and Methods of Use Thereof 审中-公开
标题翻译： RNA干扰物及其使用方法
公开(公告)号：US20130071374A1
公开(公告)日：2013-03-21
申请号：US13449095
申请日：2012-04-17
申请人： Masayori Inouye , Junjie Zhang , Yong Long Zhang
发明人： Masayori Inouye , Junjie Zhang , Yong Long Zhang
IPC分类号： C12N9/22 , C12P19/34 , A61K38/46 , C12Q1/44
CPC分类号： C12N9/22 , A61K38/465 , C12N15/70 , C12P19/34 , C12Q1/42 , C12Q1/44 , G01N2333/922 , G01N2800/26
摘要： The present invention is directed to the discovery of a novel family of enzymes designated herein as mRNA interferases that exhibit endoribonuclease activity. The novel finding of the present inventors, therefore, presents new applications for which mRNA interferase nucleic and amino acid sequences, and compositions thereof may be used to advantage. The invention also encompasses screening methods to identify compounds/agents capable of modulating mRNA interferase activity and methods for using such compounds/agents. Also provided is a kit comprising mRNA interferase nucleic and/or amino acid sequences, mRNA interferase activity compatible buffers, and instruction materials.
摘要翻译：本发明涉及在本文中称为表达内切核糖核酸酶活性的mRNA干扰酶的新型家族的发现。因此，本发明人的新发现提出了可以使用mRNA干扰酶核酸和氨基酸序列及其组合物的新用途。本发明还包括鉴定能够调节mRNA干扰酶活性的化合物/试剂的筛选方法以及使用这些化合物/试剂的方法。还提供了包含mRNA干扰酶核酸和/或氨基酸序列，mRNA干扰酶活性相容缓冲液和说明材料的试剂盒。

8. 发明申请

US20130029426A1 Dual Inducible System for the cSPP System 有权
标题翻译： cSPP系统的双诱导系统
公开(公告)号：US20130029426A1
公开(公告)日：2013-01-31
申请号：US13556100
申请日：2012-07-23
申请人： Masayori Inouye , Lili Mao , S. Thangminlal Vaiphei , Yojiro Ishida
发明人： Masayori Inouye , Lili Mao , S. Thangminlal Vaiphei , Yojiro Ishida
IPC分类号： C12N1/21 , C12N15/70
CPC分类号： C12N15/70 , C12P21/02
摘要： The present invention provides a dual inducible system for single protein production, as well as a method of inducing high level protein expression using amino acids.
摘要翻译：本发明提供了用于单蛋白生产的双诱导系统，以及使用氨基酸诱导高水平蛋白表达的方法。

9. 发明申请

US20100035346A1 SINGLE PROTEIN PRODUCTION IN LIVING CELLS FACILITATED BY A MESSENGER RNA INTERFERASE 有权
标题翻译：生物细胞中的单蛋白生产由信使RNA干扰素
公开(公告)号：US20100035346A1
公开(公告)日：2010-02-11
申请号：US11750314
申请日：2007-05-17
申请人： Masayori Inouye , Junjie Zhang , Motoo Suzuki
发明人： Masayori Inouye , Junjie Zhang , Motoo Suzuki
IPC分类号： C12N15/74 , C12N1/21
CPC分类号： C12N9/22 , C12N15/67 , C12N15/74
摘要： The present invention describes a single-protein production (SPP) system in living E. coli cells that exploits the unique properties of an mRNA interferase, for example, MazF, a bacterial toxin that is a single stranded RNA- and ACA-specific endoribonuclease, which efficiently and selectively degrades all cellular mRNAs in vivo, resulting in a precipitous drop in total protein synthesis. Concomitant expression of MazF and a target gene engineered to encode an ACA-less mRNA results in sustained and high-level (up to 90%) target expression in the virtual absence of background cellular protein synthesis. Remarkably, target synthesis continues for at least 4 days, indicating that cells retain transcriptional and translational competence despite their growth arrest. SPP technology works well for yeast and human proteins, even a bacterial integral membrane protein. This novel system enables unparalleled signal to noise ratios that should dramatically simplify structural and functional studies of previously intractable but biologically important proteins. The present invention also provides an optimized condensed single protein production system.
摘要翻译：本发明描述了利用mRNA干扰酶的独特性质的活的大肠杆菌细胞中的单蛋白质生产（SPP）系统，例如MazF，单链RNA-和ACA特异性内切核糖核酸酶的细菌毒素，其有效和选择性地降解体内所有细胞mRNA，导致总蛋白质合成的急剧下降。 MazF和旨在编码无ACA mRNA的靶基因的伴随表达导致在虚拟无背景细胞蛋白质合成中的持续和高水平（高达90％）的靶表达。值得注意的是，目标合成持续至少4天，表明细胞保留转录和翻译能力，尽管其生长停滞。 SPP技术适用于酵母和人类蛋白质，甚至细菌整合膜蛋白。这种新颖的系统能够实现无与伦比的信噪比，这将显着简化以前难以处理但生物重要的蛋白质的结构和功能研究。本发明还提供优化的冷凝单蛋白生产系统。

10. 发明申请

US20050116263A1 MULTIFUNCTIONAL BIOSENSOR BASED ON ZNO NANOSTRUCTURES 有权
标题翻译：基于ZNO纳米结构的多功能生物传感器
公开(公告)号：US20050116263A1
公开(公告)日：2005-06-02
申请号：US10456050
申请日：2003-06-06
申请人： Yicheng Lu , Zheng Zhang , Nuri Emanetoglu , Masayori Inouye , Oleg Mirochnitchenko
发明人： Yicheng Lu , Zheng Zhang , Nuri Emanetoglu , Masayori Inouye , Oleg Mirochnitchenko
IPC分类号： C12Q1/68 , G01N21/64 , G01N27/00 , G01N27/12 , G01N27/414 , G01N29/02 , G01N33/543 , H01L29/84
CPC分类号： G01N21/6428 , C12Q1/6825 , C12Q1/6837 , G01N27/4145 , G01N27/4146 , G01N29/022 , G01N33/54373 , G01N33/5438 , G01N2021/6432 , G01N2291/011 , G01N2291/014 , G01N2291/0255 , G01N2291/0256 , G01N2291/0423 , G01N2291/0426 , C12Q2565/501 , C12Q2565/607
摘要： The present invention provides the multifunctional biological and biochemical sensor technology based on ZnO nanostructures. The ZnO nanotips serve as strong DNA or protein molecule binding sites to enhance the immobilization. Patterned ZnO nanotips are used to provide conductivity-based biosensors. Patterned ZnO nanotips are also used as the gate for field-effect transistor (FET) type sensors. Patterned ZnO nanotips are integrated with SAW or BAW based biosensors. These ZnO nanotip based devices operate in multimodal operation combining electrical, acoustic and optical sensing mechanisms. The multifunctional biosensors can be arrayed and combined into one biochip, which will enhance the sensitivity and accuracy of biological and biochemical detection due to strong immobilization and multimodal operation capability. Such biological and biochemical sensor technology are useful in detection of RNA-DNA, DNA-DNA, protein-protein, protein-DNA and protein-small molecules interaction. It can be further applied for drug discovery, and for environmental monitoring and protection.
摘要翻译：本发明提供了基于ZnO纳米结构的多功能生物和生物化学传感器技术。 ZnO纳米尖端作为强的DNA或蛋白质分子结合位点来增强固定。图案化的ZnO纳米尖端用于提供基于导电性的生物传感器。图案化的ZnO纳米技术也用作场效应晶体管（FET）型传感器的栅极。图案化的ZnO纳米片与SAW或基于BAW的生物传感器集成。这些基于ZnO纳米管的器件在电气，声学和光学感测机构的多模式操作中工作。多功能生物传感器可以排列并组合成一个生物芯片，由于强固定和多模态操作能力，提高了生物和生化检测的灵敏度和准确性。这种生物和生化传感器技术可用于RNA-DNA，DNA-DNA，蛋白质 - 蛋白质，蛋白质 - DNA和蛋白质 - 小分子相互作用的检测。可进一步应用于药物发现，环境监测和保护。

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