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1. 发明申请

US20060275783A1 Gene markers for lung cancer 审中-公开
公开(公告)号：US20060275783A1
公开(公告)日：2006-12-07
申请号：US11255001
申请日：2005-10-19
申请人： Masato Mitsuhashi , Hiroko Matsunaga , Hideki Kambara , Masafumi Kawamura
发明人： Masato Mitsuhashi , Hiroko Matsunaga , Hideki Kambara , Masafumi Kawamura
IPC分类号： C12Q1/68 , A61K39/395 , G01N33/574
CPC分类号： C07H21/04 , C07K16/30 , C12Q1/6809 , C12Q1/686 , C12Q1/6886 , C12Q2600/158 , G01N33/57423 , G01N33/57484 , C12Q2539/113 , C12Q2537/162 , C12Q2525/191 , C12Q2525/15
摘要： A method for the diagnosis and identification of new or residual lung cancer is disclosed which uses newly identified markers for lung cancer including syndecan 1, collagen 1 alpha 2, and two novel proteins, 7013 and 7018. The method involves identification of the lung cancer markers is blood from a patient. It is envisioned that at least one marker may be used or any mixture of the four. The method may also include the identification of cytokeratin-19.

2. 发明授权

US07214781B2 Gene markers for lung cancer 有权
标题翻译：肺癌基因标记
公开(公告)号：US07214781B2
公开(公告)日：2007-05-08
申请号：US10297277
申请日：2001-06-21
申请人： Masato Mitsuhashi , Hiroko Matsunaga , Hideki Kambara , Masafumi Kawamura
发明人： Masato Mitsuhashi , Hiroko Matsunaga , Hideki Kambara , Masafumi Kawamura
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C07H21/04 , C07K16/30 , C12Q1/6809 , C12Q1/686 , C12Q1/6886 , C12Q2600/158 , G01N33/57423 , G01N33/57484 , C12Q2539/113 , C12Q2537/162 , C12Q2525/191 , C12Q2525/15
摘要： A method for the diagnosis and identification of new or residual lung cancer is disclosed which uses newly identified markers for lung cancer including syndecan 1, collagen 1 alpha 2, and two novel proteins, 7013 and 7018. The method involves identification of the lung cancer markers is blood from a patient. It is envisioned that at least one marker may be used or any mixture of the four. The method may also include the identification of cytokeratin-19.
摘要翻译：公开了用于诊断和鉴定新的或残留的肺癌的方法，其使用新鉴定的肺癌标志物，包括syndecan 1，胶原1α2和两种新型蛋白质，7013和7018.该方法涉及鉴定肺癌标志物是来自病人的血液。可以想象，可以使用至少一个标记物或四种的任何混合物。该方法还可以包括细胞角蛋白-19的鉴定。

3. 发明申请

US20080318244A1 Large-scale parallel nucleic acid analysis method 审中-公开
标题翻译：大规模并行核酸分析方法
公开(公告)号：US20080318244A1
公开(公告)日：2008-12-25
申请号：US12213448
申请日：2008-06-19
申请人： Hiroko Matsunaga , Hideki Kambara , Tomoharu Kajiyama
发明人： Hiroko Matsunaga , Hideki Kambara , Tomoharu Kajiyama
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6844 , C12Q2537/143 , C12Q2525/191 , C12Q2533/101 , C12Q2565/537
摘要： It is intended to provide a technique for amplifying, individually and in parallel, nucleic acids contained in a mixture of plural kinds of nucleic acid samples. The present invention provides a nucleic acid analysis method comprising amplification means, whereby amplification reaction is performed in a reaction solution comprising a homogeneous solvent and comprising at least plural template nucleic acids and solid phase carriers comprising one or more kinds of amplification probes immobilized on the surface, to prevent amplified products attributed to two or more template nucleic acids from being replicated in one solid phase carrier. According to the present invention, plural kinds of analyte nucleic acid samples in a mixed state can be amplified individually and in parallel. This method achieves one solid phase carrier-one nucleic acid. Therefore, a higher density of solid phase carriers with obtained amplified products is easily achieved, leading to improved throughput of amplified product analysis. Reactions in all the amplification reaction steps are performed under homogeneous solvent conditions. Therefore, the method of the present invention is performed by convenient procedures and as such, is suitable to automation.
摘要翻译：旨在提供用于扩增多种核酸样品的混合物中包含的核酸的单独和并行扩增的技术。本发明提供了包含扩增方法的核酸分析方法，其中扩增反应在包含均相溶剂并包含至少多个模板核酸的反应溶液中进行，并且包含固定在表面上的一种或多种扩增探针的固相载体，以防止归因于两种或更多种模板核酸的扩增产物被复制在一种固相载体中。根据本发明，可以单独并行地扩增处于混合状态的多种分析物核酸样品。该方法实现了一种固相载体一核酸。因此，容易获得具有获得的扩增产物的较高密度的固相载体，从而提高扩增产物分析的产量。所有扩增反应步骤中的反应在均相溶剂条件下进行。因此，本发明的方法通过方便的程序进行，因此适用于自动化。

4. 发明授权

US06183970B2 Polynucleotide probe chip and polynucleotide detection method 有权
标题翻译：多核苷酸探针芯片和多核苷酸检测方法
公开(公告)号：US06183970B2
公开(公告)日：2001-02-06
申请号：US09383198
申请日：1999-08-26
申请人： Kazunori Okano , Hideki Kambara , Chihiro Uematsu , Hiroko Matsunaga , Takashi Irie , Tomoharu Kajiyama , Kenji Yasuda
发明人： Kazunori Okano , Hideki Kambara , Chihiro Uematsu , Hiroko Matsunaga , Takashi Irie , Tomoharu Kajiyama , Kenji Yasuda
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00317 , B01J2219/00576 , B01J2219/00596 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00677 , B01J2219/00702 , B01J2219/00704 , B01J2219/00713 , B01J2219/00722 , B01L3/5085 , B01L3/5088 , B01L2300/0819 , B01L2400/0421 , C12Q1/6837 , C40B40/06 , C12Q2565/607 , C12Q2561/119
摘要： There are beforehand prepared a monomer having a reaction residue and a polynucleotide probe set comprising plural kinds of polynucleotide probes having a residue bonded to the reaction residue. The monomer is mixed with each kind of polynucleotide probes comprising any plural probes selected from the polynucleotide probe set. Each kind of the resultant mixtures is added to each of different small holes to make the mixture into gel matrix. Thus, a polynucleotide probe chip is produced. Sample DNA is forcibly migrated in the gels by electrophoresis. Laser light is projected onto the side face of the chip. The fluorescence emitted from the whole surface of the chip is collectively detected with a high-sensitive two-dimensional detector. Thus, the polynucleotide probe chip, holding various kinds of DNA probes, for detecting DNA can be provided. This chip has high hybridization-efficiency and makes high-sensitivity and high-speed DNA detection possible.
摘要翻译：预先制备具有反应残基的单体和含有残基与反应残基连接的多种多核苷酸探针的多核苷酸探针组。将单体与包含选自多核苷酸探针组的任何多个探针的各种多核苷酸探针混合。将各种所得混合物加入每个不同的小孔中以使混合物成为凝胶基质。因此，产生多核苷酸探针芯片。样品DNA通过电泳在凝胶中强制迁移。激光投射到芯片的侧面。从芯片的整个表面发射的荧光由高灵敏度二维检测器共同检测。因此，可以提供用于检测DNA的保持各种DNA探针的多核苷酸探针芯片。该芯片具有高杂交效率，可实现高灵敏度和高速DNA检测。

5. 发明授权

US06514702B1 Polynucleotide chip probe and polynucleotide detection method 失效
标题翻译：多核苷酸芯片探针和多核苷酸检测方法
公开(公告)号：US06514702B1
公开(公告)日：2003-02-04
申请号：US09670701
申请日：2000-09-28
申请人： Kazunori Okano , Hideki Kambara , Chihiro Uematsu , Hiroko Matsunaga , Takashi Irie , Tomoharu Kajiyama , Kenji Yasuda
发明人： Kazunori Okano , Hideki Kambara , Chihiro Uematsu , Hiroko Matsunaga , Takashi Irie , Tomoharu Kajiyama , Kenji Yasuda
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00317 , B01J2219/00576 , B01J2219/00596 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00677 , B01J2219/00702 , B01J2219/00704 , B01J2219/00713 , B01J2219/00722 , B01L3/5085 , B01L3/5088 , B01L2300/0819 , B01L2400/0421 , C12Q1/6837 , C40B40/06 , C12Q2565/607 , C12Q2561/119
摘要： There are beforehand prepared a monomer having a reaction residue and a polynucleotide probe set comprising plural kinds of polynucleotide probes having a residue bonded to the reaction residue. The monomer is mixed with each kind of polynucleotide probes comprising any plural probes selected from the polynucleotide probe set. Each kind of the resultant mixtures is added to each of different small holes to make the mixture into gel matrix. Thus, a polynucleotide probe chip is produced. Sample DNA is forcibly migrated in the gels by electrophoresis. Laser light is projected onto the side face of the chip. The fluorescence emitted from the whole surface of the chip is collectively detected with a high-sensitive two-dimensional detector. Thus, the polynucleotide probe chip, holding various kinds of DNA probes, for detecting DNA can be provided. This chip has high hybridization-efficiency and makes high-sensitivity and high-speed DNA detection possible.
摘要翻译：预先制备具有反应残基的单体和含有残基与反应残基连接的多种多核苷酸探针的多核苷酸探针组。将单体与包含选自多核苷酸探针组的任何多个探针的各种多核苷酸探针混合。将各种所得混合物加入每个不同的小孔中以使混合物成为凝胶基质。因此，产生多核苷酸探针芯片。样品DNA通过电泳在凝胶中强制迁移。激光投射到芯片的侧面。从芯片的整个表面发射的荧光由高灵敏度二维检测器共同检测。因此，可以提供用于检测DNA的保持各种DNA探针的多核苷酸探针芯片。该芯片具有高杂交效率，可实现高灵敏度和高速DNA检测。

6. 发明授权

US5968743A DNA sequencing method and reagents kit 失效
标题翻译： DNA测序方法和试剂盒
公开(公告)号：US5968743A
公开(公告)日：1999-10-19
申请号：US948364
申请日：1997-10-09
申请人： Hiroko Matsunaga , Kazunori Okano , Hideki Kambara
发明人： Hiroko Matsunaga , Kazunori Okano , Hideki Kambara
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6869
摘要： The present invention provides a DNA sequencing method comprising: (1) a step of fragmentation of a sample DNA and amplifying each fragment to obtain a first DNA fragment; (2) a step of obtaining from the first DNA fragment a second DNA fragment substantially complementary to the sample DNA at least at the 3' terminus thereof; and (3) a step of performing an extension reaction of complementary strand, using the sample DNA as a template to produce a third DNA fragment containing a base sequence complementary to the second DNA fragment and having a size longer than that of the second DNA fragment, and using the third DNA fragment as a template for sequencing of the sample DNA. DNA sequencing can be proceeded efficiently with extremely low redundancy.
摘要翻译：本发明提供一种DNA测序方法，其特征在于，包括：（1）将样品DNA分解并扩增各片段以获得第一DNA片段的步骤; （2）至少在其3'末端从第一DNA片段获得与样品DNA基本互补的第二DNA片段的步骤; 和（3）使用样品DNA作为模板进行互补链的延伸反应的步骤，以产生含有与第二个DNA片段互补的碱基序列并且具有比第二个DNA片段长的碱基序列的第三个DNA片段，并使用第三个DNA片段作为样品DNA测序的模板。可以以极低的冗余度有效地进行DNA测序。

7. 发明授权

US07670992B2 Method of producing probe arrays for biological materials using fine particles 失效
标题翻译：使用细颗粒生产生物材料的探针阵列的方法
公开(公告)号：US07670992B2
公开(公告)日：2010-03-02
申请号：US11484046
申请日：2006-07-10
申请人： Hideki Kambara , Masato Mitsuhashi
发明人： Hideki Kambara , Masato Mitsuhashi
IPC分类号： C40B40/00 , G01N33/543 , G01N33/551 , G01N33/544
CPC分类号： B01L3/5027 , B01J19/0046 , B01J2219/00286 , B01J2219/00308 , B01J2219/00337 , B01J2219/00364 , B01J2219/00367 , B01J2219/00405 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/00596 , B01J2219/00657 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , B01L2200/0631 , B01L2200/12 , B01L2300/0636 , B01L2300/0816 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/54313
摘要： The use of probe arrays in which probes of various biological substances such as DNA are immobilized on the surface of a solid is becoming established as an effective means for high-speed screening. Different kinds of probes, such as DNA, are immobilized on the surface of a multiple number of independently treatable fine particles, such as beads, instead of the surface of a single solid, and the resulting beads are aligned in a capillary or a cell in a designated order. The size of the area where one probe is immobilized is reduced. The bead probe array is characterized in that such small beads are aligned one by one in a designated manner using a sheet with holes, and one or a multiple number of beads are held in the holes and then transferred to a probe array holder such as a capillary.
摘要翻译：使用其中各种生物物质如DNA的探针固定在固体表面上的探针阵列正在成为高速筛选的有效手段。将不同种类的探针（例如DNA）固定在多个可独立处理的细颗粒（例如珠粒）的表面上而不是单个固体的表面上，并将所得的珠粒在毛细管或细胞中排列指定的订单。一个探针被固定的区域的尺寸减小了。珠探针阵列的特征在于，使用具有孔的片以指定的方式一个接一个地排列这样的小珠，并且将一个或多个珠保持在孔中，然后转移到探针阵列保持器，例如毛细管。

8. 发明申请

US20060275818A1 Method of producing probe arrays for biological materials using fine particles 失效
标题翻译：使用细颗粒生产生物材料的探针阵列的方法
公开(公告)号：US20060275818A1
公开(公告)日：2006-12-07
申请号：US11484046
申请日：2006-07-10
申请人： Hideki Kambara , Masato Mitsuhashi
发明人： Hideki Kambara , Masato Mitsuhashi
IPC分类号： C40B30/06 , C40B40/08
CPC分类号： B01L3/5027 , B01J19/0046 , B01J2219/00286 , B01J2219/00308 , B01J2219/00337 , B01J2219/00364 , B01J2219/00367 , B01J2219/00405 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/00596 , B01J2219/00657 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , B01L2200/0631 , B01L2200/12 , B01L2300/0636 , B01L2300/0816 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/54313
摘要： The use of probe arrays in which probes of various biological substances such as DNA are immobilized on the surface of a solid is becoming established as an effective means for high-speed screening. Different kinds of probes, such as DNA, are immobilized on the surface of a multiple number of independently treatable fine particles, such as beads, instead of the surface of a single solid, and the resulting beads are aligned in a capillary or a cell in a designated order. The size of the area where one probe is immobilized is reduced. The bead probe array is characterized in that such small beads are aligned one by one in a designated manner using a sheet with holes, and one or a multiple number of beads are held in the holes and then transferred to a probe array holder such as a capillary.
摘要翻译：使用其中各种生物物质如DNA的探针固定在固体表面上的探针阵列正在成为高速筛选的有效手段。将不同种类的探针（例如DNA）固定在多个可独立处理的细颗粒（例如珠粒）的表面上而不是单个固体的表面上，并将所得的珠粒在毛细管或细胞中排列指定的订单。一个探针被固定的区域的尺寸减小了。珠探针阵列的特征在于，使用具有孔的片以指定的方式一个接一个地排列这样的小珠，并且将一个或多个珠保持在孔中，然后转移到探针阵列保持器，例如毛细管。

9. 发明申请

US20050100943A1 Method of producing probe arrays for biological materials using fine particles 审中-公开
标题翻译：使用细颗粒生产生物材料的探针阵列的方法
公开(公告)号：US20050100943A1
公开(公告)日：2005-05-12
申请号：US10964602
申请日：2004-10-12
申请人： Hideki Kambara , Masato Mitsuhashi
发明人： Hideki Kambara , Masato Mitsuhashi
IPC分类号： B01J19/00 , B05D3/00 , C12M1/34 , C12Q1/68 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/543
CPC分类号： B01L3/5027 , B01J19/0046 , B01J2219/00286 , B01J2219/00308 , B01J2219/00337 , B01J2219/00364 , B01J2219/00367 , B01J2219/00405 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/00596 , B01J2219/00657 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , B01L2200/0631 , B01L2200/12 , B01L2300/0636 , B01L2300/0816 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/54313
摘要： The use of probe arrays in which probes of various biological substances such as DNA are immobilized on the surface of a solid is becoming established as an effective means for high-speed screening. Different kinds of probes, such as DNA, are immobilized on the surface of a multiple number of independently treatable fine particles, such as beads, instead of the surface of a single solid, and the resulting beads are aligned in a capillary or a cell in a designated order. The size of the area where one probe is immobilized is reduced. The bead probe array is characterized in that such small beads are aligned one by one in a designated manner using a sheet with holes, and one or a multiple number of beads are held in the holes and then transferred to a probe array holder such as a capillary.
摘要翻译：使用其中各种生物物质如DNA的探针固定在固体表面上的探针阵列正在成为高速筛选的有效手段。将不同种类的探针（例如DNA）固定在多个可独立处理的细颗粒（例如珠粒）的表面上而不是单个固体的表面上，并将所得的珠粒在毛细管或细胞中排列指定的订单。一个探针被固定的区域的尺寸减小了。珠探针阵列的特征在于，使用具有孔的片以指定的方式一个接一个地排列这样的小珠，并且将一个或多个珠保持在孔中，然后转移到探针阵列保持器，例如毛细管。

10. 发明授权

US08975066B2 DNA analysis apparatus 有权
标题翻译： DNA分析仪器
公开(公告)号：US08975066B2
公开(公告)日：2015-03-10
申请号：US12419399
申请日：2009-04-07
申请人： Hideki Kambara , Akihiko Kishimoto
发明人： Hideki Kambara , Akihiko Kishimoto
IPC分类号： C12M1/34 , C12M3/00 , C12Q1/68 , B01L7/00
CPC分类号： C12Q1/6869 , B01J2219/00659 , B01J2219/00722 , B01L7/52 , C12Q2565/301 , C12Q2563/103 , C12Q2535/101
摘要： Accurate and sensitive sequencing in pyrosequencing is achieved by allowing complementary strand synthesis reaction to proceed homogeneously and completely in a short time while performing luminescence reaction for a sufficiently long time. DNA as a sequencing target is immobilized on the surface of a solid support. Nucleic acid substrates are injected from a dispenser to the support site where complementary strand synthesis is in turn performed rapidly and completely in a short time under a small reaction volume. Next, the support together with the product thereon is moved into a luminescence reaction solution where luminescence reaction is in turn performed. Thus, a DNA complementary strand synthesis reaction site and a luminescence reaction site are completely separated. The support surface is also washed by dipping the support in the luminescence reaction solution that contains a luminescence reagent and an enzyme that degrades redundant nucleic acid substrates.
摘要翻译：焦磷酸测序中的精确和灵敏的测序通过使互补链合成反应在短时间内均匀且完全地进行，同时进行发光反应足够长的时间来实现。作为测序靶的DNA被固定在固体支持物的表面上。将核酸底物从分配器注射到支持部位，其中互补链合成在短时间内在小反应体积下快速且完全地进行。接下来，将载体与其上的产物一起移动到进行发光反应的发光反应溶液中。因此，DNA互补链合成反应位点和发光反应位点完全分离。通过将载体浸渍在含有发光试剂的发光反应溶液和降解冗余核酸底物的酶中也可以洗涤载体表面。

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