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1. 发明授权

US07486440B2 Polarized total internal reflection illumination optical system by rotary annulus light 失效
标题翻译：通过旋转环空光学偏振全内反射照明光学系统
公开(公告)号：US07486440B2
公开(公告)日：2009-02-03
申请号：US10539757
申请日：2003-09-18
申请人： Masasuke Yoshida , Toshiharu Suzuki , Megumi Shio , Ryota Iino
发明人： Masasuke Yoshida , Toshiharu Suzuki , Megumi Shio , Ryota Iino
IPC分类号： G02B21/06
CPC分类号： G02B21/084 , G02B21/06
摘要： An illumination system uses rotatable, polarized illumination optics to detect the direction of highly efficient excitation of fluorescent dyes coupled to a sample, or the absorption transition moment, using information on the direction of maximal fluorescence intensity, in which it is possible to detect individual dynamic changes in the internal structure or orientation of an entire protein molecule by coupling a single fluorescent dye molecule to the protein molecule. The polarized total internal reflection illumination optical system by rotary annulus light is also an illumination optical system in which laser beams are introduced into the objective lens of a microscope at the peripheral region by means of the rotatable illumination direction of the laser beams, and is designed to illuminate by the evanescent field that contains only transverse components that are perpendicular to the direction of radiation from the optical axis of the objective lens.
摘要翻译：照明系统使用可旋转的偏振照明光学器件来检测耦合到样品的荧光染料的高效激发的方向或吸收转变时刻，使用关于最大荧光强度方向的信息，其中可以检测单个动态通过将单个荧光染料分子偶联到蛋白质分子上，整个蛋白质分子的内部结构或取向的变化。通过旋转环带光的极化全内反射照明光学系统也是一种照明光学系统，其中激光束通过激光束的可旋转照明方向在周边区域被引入显微镜的物镜中，并被设计由仅包含垂直于物镜的光轴的辐射方向的横向分量的渐逝场照亮。

2. 发明申请

US20080062513A1 Polarized Total Internal Reflection Illumination Optical System by Rotary Annulus Light 失效
标题翻译：旋转环形光的极化全内反射照明光学系统
公开(公告)号：US20080062513A1
公开(公告)日：2008-03-13
申请号：US10539757
申请日：2003-09-18
申请人： Masasuke Yoshida , Toshiharu Suzuki , Megumi Shio , Ryota Iino
发明人： Masasuke Yoshida , Toshiharu Suzuki , Megumi Shio , Ryota Iino
IPC分类号： G02B21/06
CPC分类号： G02B21/084 , G02B21/06
摘要： The present invention relates to an illumination system that use rotatable, polarized illumination optics to detect the direction of highly efficient excitation of fluorescent dyes coupled to a sample, or the absorption transition moment, using information on the direction of maximal fluorescence intensity. The present invention also makes it possible to detect individual dynamic changes in the internal structure or orientation of an entire protein molecule by coupling a single fluorescent dye molecule to the protein molecule. The polarized total internal reflection illumination optical system by rotary annulus light of the present invention is also an illumination optical system in which laser beams are introduced into the objective lens of a microscope at the peripheral region by means of the rotatable illumination direction of the laser beams, and the present invention is designed to illuminate by the evanescent field that contains only transverse components that are perpendicular to the direction of radiation from the optical axis of the objective lens. The present invention is also an illumination optical system in which the direction of vibration of the rotating polarized light, which are not usually visualized, is easily detectable in the visual field of a microscope by providing a index pin in the illumination system, while additionally the rotating speed of the polarized light can be monitored from the rotary drive control system.
摘要翻译：本发明涉及使用可旋转的偏振照明光学器件来检测耦合到样品的荧光染料的高效激发方向或吸收转变时刻的照明系统，使用关于最大荧光强度方向的信息。本发明还可以通过将单个荧光染料分子偶联到蛋白质分子来检测整个蛋白质分子的内部结构或取向的单个动态变化。本发明的通过旋转环形光的极化全内反射照明光学系统也是一种照明光学系统，其中激光束通过激光束的可旋转照明方向在周边区域被引入显微镜的物镜并且本发明被设计为通过仅包含垂直于从物镜的光轴的辐射方向的横向分量的渐逝场照射。本发明还是一种照明光学系统，其中通常在显微镜的视场中通常可视化的旋转偏振光的振动方向在照明系统中提供一个折射引脚，而另外可以从旋转驱动控制系统监视偏振光的旋转速度。

3. 发明授权

US09399788B2 Method for inspecting susceptibility of bacteria or fungi to antimicrobial drug and system for use in the same 有权
标题翻译：用于检测细菌或真菌对抗菌药物的敏感性的方法及其用途
公开(公告)号：US09399788B2
公开(公告)日：2016-07-26
申请号：US14344475
申请日：2012-08-31
申请人： Yoshimi Matsumoto , Kohei Hayama , Shouichi Sakakihara , Kunihiko Nishino , Akihito Yamaguchi , Hiroyuki Noji , Ryota Iino
发明人： Yoshimi Matsumoto , Kohei Hayama , Shouichi Sakakihara , Kunihiko Nishino , Akihito Yamaguchi , Hiroyuki Noji , Ryota Iino
IPC分类号： C12Q1/18
CPC分类号： C12Q1/18
摘要： The present disclosure describes a method capable of easily and rapidly inspecting the susceptibility of bacteria or fungi to an antimicrobial drug. The inspection method of the present disclosure is a method for inspecting susceptibility of bacteria or fungi to an antimicrobial drug using a micro-device having flow channels, including: incubating a mixture of the antimicrobial drug and a suspension to be inspected in the flow channels of the micro-device; and detecting bacteria or fungi derived from the suspension to be inspected in an observation area of the flow channels of the micro-device. The detecting step can be performed by detecting an increase or decrease in the number of or a change in shape of bacteria or fungi derived from the suspension to be inspected in the observation area by a microscope or the like.
摘要翻译：本公开描述了能够容易且快速地检查细菌或真菌对抗微生物药物的敏感性的方法。本公开的检查方法是使用具有流动通道的微型装置检测细菌或真菌对抗微生物药物的敏感性的方法，包括：将抗微生物药物和待检查的悬浮液的混合物在流动通道微器件; 以及在微型装置的流路的观察区域中检测来自要检查的悬浮液的细菌或真菌。检测步骤可以通过用显微镜等检测在观察区域中待检查的悬浮液的细菌或真菌的数量的增加或变化的形状的增加或减少来进行。

4. 发明授权

US08524447B2 Fluorescently labeled fusion protein for assaying adenosine triphosphate 有权
标题翻译：荧光标记的融合蛋白，用于测定三磷酸腺苷
公开(公告)号：US08524447B2
公开(公告)日：2013-09-03
申请号：US12594198
申请日：2008-03-25
申请人： Hiroyuki Noji , Hiromi Imamura , Ryota Iino , Yasuyuki Yamada
发明人： Hiroyuki Noji , Hiromi Imamura , Ryota Iino , Yasuyuki Yamada
IPC分类号： C12Q1/68 , C12Q1/02 , C12N9/00 , C12N15/63
CPC分类号： C07K14/00 , C07K14/32 , C07K2319/60 , C12N9/14 , G01N33/533 , G01N33/542 , G01N33/573 , G01N33/582 , G01N2333/914
摘要： The object of the present invention is to provide a substance, which is easy to handle and enables the measurement of ATP with a high sensitivity regardless of the concentration of protein, and further a measuring method of ATP using the substance. Such object is solved with a fluorescence labelled fusion protein obtained by attaching two types of fluorescent substances of potential donor and acceptor for fluorescence resonance energy transfer (FRET) respectively to a protein which can cause structural changes depending on ATP binding, namely ε protein, which is the subunit of ATP synthetase, and further solved by contacting the fluorescence labelled fusion protein with a subject substance and then measuring the fluorescence spectra.
摘要翻译：本发明的目的是提供一种容易处理的物质，并且能够高灵敏度地测量ATP，而与蛋白质的浓度无关，并且还提供了使用该物质的ATP的测量方法。通过将两种类型的荧光共振能量转移（FRET）的潜在供体荧光物质和受体的荧光物质分别连接到能够引起结构变化（依赖于ATP结合的蛋白质，即ε蛋白）而得到的荧光标记的融合蛋白来解决这个问题。是ATP合成酶的亚基，进一步通过荧光标记的融合蛋白与目标物质接触，然后测量荧光光谱。

5. 发明申请

US20100233692A1 FLUORESCENTLY LABELED FUSION PROTEIN FOR ASSAYING ADENOSINE TRIPHOSPHATE 有权
标题翻译：荧光标记的融合蛋白，用于测定腺嘌呤三磷酸
公开(公告)号：US20100233692A1
公开(公告)日：2010-09-16
申请号：US12594198
申请日：2008-03-25
申请人： Hiroyuki Noji , Hiromi Imamura , Ryota Iino , Yasuyuki Yamada
发明人： Hiroyuki Noji , Hiromi Imamura , Ryota Iino , Yasuyuki Yamada
IPC分类号： C12Q1/68 , C12Q1/02 , C12N9/00 , C12N15/63 , C07H21/04
CPC分类号： C07K14/00 , C07K14/32 , C07K2319/60 , C12N9/14 , G01N33/533 , G01N33/542 , G01N33/573 , G01N33/582 , G01N2333/914
摘要： The object of the present invention is to provide a substance, which is easy to handle and enables the measurement of ATP with a high sensitivity regardless of the concentration of protein, and further a measuring method of ATP using the substance. Such object is solved with a fluorescence labelled fusion protein obtained by attaching two types of fluorescent substances of potential donor and acceptor for fluorescence resonance energy transfer (FRET) respectively to a protein which can cause structural changes depending on ATP binding, namely ε protein, which is the subunit of ATP synthetase, and further solved by contacting the fluorescence labelled fusion protein with a subject substance and then measuring the fluorescence spectra.
摘要翻译：本发明的目的是提供一种容易处理的物质，并且能够高灵敏度地测量ATP，而与蛋白质的浓度无关，并且还提供了使用该物质的ATP的测量方法。通过将两种荧光共振能量转移（FRET）的潜在供体荧光物质和受体的荧光物质分别连接到能够引起结构上依赖于ATP结合的结构变化的蛋白质而获得的荧光标记的融合蛋白来解决这个问题。蛋白质，其是ATP合成酶的亚基，并且通过将荧光标记的融合蛋白与受试物质接触，然后测量荧光光谱进一步解决。

6. 发明申请

US20130345088A1 BEAD SEALING METHOD, METHOD FOR DETECTING TARGET MOLECULE, ARRAY, KIT, AND TARGET MOLECULE DETECTION DEVICE 有权
标题翻译：珠密封方法，用于检测目标分子的方法，阵列，试剂盒和目标分子检测装置
公开(公告)号：US20130345088A1
公开(公告)日：2013-12-26
申请号：US14003509
申请日：2012-03-07
申请人： Hiroyuki Noji , Ryota Iino , Suguru Araki
发明人： Hiroyuki Noji , Ryota Iino , Suguru Araki
IPC分类号： G01N33/543
CPC分类号： G01N33/54386 , B01J2219/00317 , B01J2219/00466 , B01J2219/00619 , B01J2219/00621 , B01L3/502715 , B01L3/502761 , B01L3/50853 , B01L2200/0642 , B01L2200/0668 , B01L2200/12 , B01L2300/0627 , B01L2300/0819 , B01L2300/0829 , B01L2300/0858 , B01L2300/0877 , B01L2300/161 , B01L2300/165 , G01N21/6452 , G01N33/54306 , G01N33/54313
摘要： This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41′) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41′) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).
摘要翻译：本发明提供能够以高灵敏度检测低浓度的目标分子的技术。本发明包括（i）将含有珠（40），（41'）的亲水性溶剂（42）引入到（a）包括多个容器（13）的下层部分（10）之间的空间（30）），其每一个仅能够容纳一个珠（41），（41'），并且通过具有疏水性上表面的侧壁（12）和（b）上层部分（20'）彼此分离）面向设置有多个插座（13）的表面的下层部分（10）的表面; 和（ii）将疏水性溶剂（43）引入空间（30）的步骤，步骤（ii）在步骤（i）之后进行。

7. 发明授权

US09329174B2 Bead trapping method and method for detecting target molecule 有权
标题翻译：珠粒捕获法和靶分子检测方法
公开(公告)号：US09329174B2
公开(公告)日：2016-05-03
申请号：US14003509
申请日：2012-03-07
申请人： Hiroyuki Noji , Ryota Iino , Suguru Araki
发明人： Hiroyuki Noji , Ryota Iino , Suguru Araki
IPC分类号： G01N33/543 , G01N21/64 , B01L3/00
CPC分类号： G01N33/54386 , B01J2219/00317 , B01J2219/00466 , B01J2219/00619 , B01J2219/00621 , B01L3/502715 , B01L3/502761 , B01L3/50853 , B01L2200/0642 , B01L2200/0668 , B01L2200/12 , B01L2300/0627 , B01L2300/0819 , B01L2300/0829 , B01L2300/0858 , B01L2300/0877 , B01L2300/161 , B01L2300/165 , G01N21/6452 , G01N33/54306 , G01N33/54313
摘要： This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41′) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41′) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).
摘要翻译：本发明提供能够以高灵敏度检测低浓度的目标分子的技术。本发明包括（i）将含有珠（40），（41'）的亲水性溶剂（42）引入到（a）包括多个容器（13）的下层部分（10）之间的空间（30）），其每一个仅能够容纳一个珠（41），（41'），并且通过具有疏水性上表面的侧壁（12）和（b）上层部分（20'）彼此分离）面向设置有多个插座（13）的表面的下层部分（10）的表面; 和（ii）将疏水性溶剂（43）引入空间（30）的步骤，步骤（ii）在步骤（i）之后进行。

8. 发明申请

US20140349333A1 METHOD FOR INSPECTING SUSCEPTIBILITY OF BACTERIA OR FUNGI TO ANTIMICROBIAL DRUG AND SYSTEM FOR USE IN THE SAME 有权
标题翻译：检测细菌或真菌对抗微生物药物及其用途的系统的检测方法
公开(公告)号：US20140349333A1
公开(公告)日：2014-11-27
申请号：US14344475
申请日：2012-08-31
申请人： Yoshimi Matsumoto , Kohei Hayama , Shouichi Sakakihara , Kunihiko Nishino , Akihito Yamaguchi , Hiroyuki Noji , Ryota Iino
发明人： Yoshimi Matsumoto , Kohei Hayama , Shouichi Sakakihara , Kunihiko Nishino , Akihito Yamaguchi , Hiroyuki Noji , Ryota Iino
IPC分类号： C12Q1/18
CPC分类号： C12Q1/18
摘要： The present invention provides a novel method capable of easily and rapidly inspecting the susceptibility of bacteria or fungi to an antimicrobial drug and an inspection system for use in the method. The inspection method of the present invention is a method for inspecting susceptibility of bacteria or fungi to an antimicrobial drug using a micro-device having a flow channel, including: an incubating step of incubating a mixture of the antimicrobial drug and a bacterial suspension to be inspected in the flow channel of the micro-device; and a detecting step of detecting bacteria or fungi derived from the bacterial suspension to be inspected in an observation area of the flow channel of the micro-device. The detecting step can be performed by detecting an increase or decrease in the number of or a change in shape of bacteria or fungi derived from the bacterial suspension to be inspected in the observation area by a microscope or the like.
摘要翻译：本发明提供了能够容易且快速地检测细菌或真菌对抗微生物药物的敏感性和用于该方法的检查系统的新方法。本发明的检查方法是使用具有流路的微型装置检测细菌或真菌对抗微生物药物的敏感性的方法，包括：将抗微生物药物和细菌悬浮液的混合物孵育成的孵育步骤，在微型装置的流路中检查; 以及检测步骤，在微型装置的流路的观察区域中检测来自待检查的细菌悬浮液的细菌或真菌。检测步骤可以通过显微镜等检测观察区域中待检查的细菌悬浮液的细菌或真菌的数量的增加或变化的形状的增加或减少来进行。

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