专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20050239045A1 Microorganism or cell collecting method, and microorganism or cell collecting implement used for the method 审中-公开
标题翻译：微生物或细胞收集方法，以及用于该方法的微生物或细胞收集器具
公开(公告)号：US20050239045A1
公开(公告)日：2005-10-27
申请号：US10522045
申请日：2003-12-08
申请人： Masashi Okamoto , Tatsuo Kamata , Yuji Izumizawa , Atsushi Murakami
发明人： Masashi Okamoto , Tatsuo Kamata , Yuji Izumizawa , Atsushi Murakami
IPC分类号： C12N1/02 , C12N1/20 , C12Q1/00 , C12Q1/24
CPC分类号： C12Q1/24 , C12N1/02 , G01N2333/35
摘要： A method capable of easily collecting microorganisms or cells in a short time without using a special device, comprising the steps of preparing a centrifugal tube (1) in which the inside thereof is divided into upper and lower parts by a filter (14) and water absorbing resin particles (15) are disposed on the filter (14), pouring liquid specimen in the centrifugal tube (1) so that the liquid specimen touches the water absorbing resin particles (15) to absorb the liquid specimen by the resin particles so as to arrest microorganisms or cells on the surfaces of the particles, pouring collecting liquid into the centrifugal tube (1) to collect the microorganisms or cells with the collecting liquid and, by centrifugal separation, accumulating the collecting liquid at the bottom part of the centrifugal tube (1) through the filter (14).
摘要翻译：一种能够在不使用特殊装置的情况下在短时间内容易地收集微生物或细胞的方法，其特征在于，包括以下步骤：制备其中内部通过过滤器（14）分成上部和下部的离心管（1）和水吸收树脂颗粒（15）设置在过滤器（14）上，将液体样本倾倒在离心管（1）中，使得液体样本接触吸水树脂颗粒（15）以吸收树脂颗粒的液体样本，以便阻止颗粒表面上的微生物或细胞，将收集液倒入离心管（1）中，用收集液收集微生物或细胞，并通过离心分离将收集液体积聚在离心管底部（1）通过过滤器（14）。

2. 发明授权

US07935483B2 Method of effecting lysis of acid-fast bacteria and method of performing gene amplification or detection therewith 有权
标题翻译：实施耐酸细菌裂解的方法及其进行基因扩增或检测的方法
公开(公告)号：US07935483B2
公开(公告)日：2011-05-03
申请号：US11656575
申请日：2007-01-23
申请人： Tatsuo Kamata , Yuji Izumizawa
发明人： Tatsuo Kamata , Yuji Izumizawa
IPC分类号： C12Q1/68
CPC分类号： C12N15/1003 , C12Q1/6806
摘要： A method of effecting lysis of acid-fast bacteria, comprising heating acid-fast bacteria in a liquid containing a non-ionic surfactant at a temperature of below the boiling point of the liquid. This method enables accomplishing secure lysis of acid-fast bacteria in a simple manner within a short period of time without the use of special apparatus and agent and enables extracting genes. The heating is preferably conducted at 96° C. for 10 min. As the nonionic surfactant, use can be made of a d-sorbitol fatty acid ester, a polyoxyethylene glycol sorbitan alkyl ester, a polyoxyethylene glycol p-t-octylphenyl ether or the like. The pH value of the liquid is preferably 8, and the liquid preferably contains EDTA. It is also preferred that before the heating, the acid-fast bacteria be treated with lipase.
摘要翻译：一种实现耐酸菌细菌裂解的方法，包括在低于液体沸点的温度下，将含有非离子表面活性剂的液体中的耐酸细菌加热。该方法能够在短时间内以简单的方式实现耐酸菌的安全裂解，而无需使用专门的装置和试剂，能够提取基因。加热优选在96℃进行10分钟。作为非离子表面活性剂，可以使用d-山梨糖醇脂肪酸酯，聚氧乙烯二醇脱水山梨醇烷基酯，聚氧乙烯二醇对叔辛基苯基醚等。液体的pH值优选为8，液体优选含有EDTA。还优选在加热之前，用脂肪酶处理耐酸细菌。

3. 发明申请

US20070178508A1 Method of effecting lysis of acid-fast bacteria and method of performing gene amplification or detection therewith 有权
标题翻译：实施耐酸细菌裂解的方法及其进行基因扩增或检测的方法
公开(公告)号：US20070178508A1
公开(公告)日：2007-08-02
申请号：US11656575
申请日：2007-01-23
申请人： Tatsuo Kamata , Yuji Izumizawa
发明人： Tatsuo Kamata , Yuji Izumizawa
IPC分类号： C12Q1/68 , C12N1/08 , C12P19/34
CPC分类号： C12N15/1003 , C12Q1/6806
摘要： A method of effecting lysis of acid-fast bacteria, comprising heating acid-fast bacteria in a liquid containing a non-ionic surfactant at a temperature of below the boiling point of the liquid. This method enables accomplishing secure lysis of acid-fast bacteria in a simple manner within a short period of time without the use of special apparatus and agent and enables extracting genes. The heating is preferably conducted at 96° C. for 10 min. As the nonionic surfactant, use can be made of a d-sorbitol fatty acid ester, a polyoxyethylene glycol sorbitan alkyl ester, a polyoxyethylene glycol p-t-octylphenyl ether or the like. The pH value of the liquid is preferably 8, and the liquid preferably contains EDTA. It is also preferred that before the heating, the acid-fast bacteria be treated with lipase.
摘要翻译：一种实现耐酸菌细菌裂解的方法，包括在低于液体沸点的温度下，将含有非离子表面活性剂的液体中的耐酸细菌加热。该方法能够在短时间内以简单的方式实现耐酸菌的安全裂解，而无需使用专门的装置和试剂，能够提取基因。加热优选在96℃进行10分钟。作为非离子表面活性剂，可以使用d-山梨糖醇脂肪酸酯，聚氧乙烯二醇脱水山梨醇烷基酯，聚氧乙烯二醇对叔辛基苯基醚等。液体的pH值优选为8，液体优选含有EDTA。还优选在加热之前，用脂肪酶处理耐酸细菌。

4. 发明申请

US20050181363A1 Method of effecting lysis of acid-fast bacteria and method of performing gene amplification or detection therewith 审中-公开
标题翻译：实施耐酸细菌裂解的方法及其进行基因扩增或检测的方法
公开(公告)号：US20050181363A1
公开(公告)日：2005-08-18
申请号：US10500435
申请日：2003-05-21
申请人： Tatsuo Kamata , Yuji Izumizawa
发明人： Tatsuo Kamata , Yuji Izumizawa
IPC分类号： C12N1/06 , C12N15/10 , C12Q1/68 , C12N1/08
CPC分类号： C12N15/1003 , C12Q1/6806
摘要： A method of effecting lysis of acid-fast bacteria, comprising heating acid-fast bacteria in a liquid containing a non-ionic surfactant at a temperature of below the boiling point of the liquid. This method enables accomplishing secure lysis of acid-fast bacteria in a simple manner within a short period of time without the use of special apparatus and agent and enables extracting genes. The heating is preferably conducted at 96° C. for 10 min. As the nonionic surfactant, use can be made of a d-sorbitol fatty acid ester, a polyoxyethylene glycol sorbitan alkyl ester, a polyoxyethylene glycol p-t-octylphenyl ether or the like. The pH value of the liquid is preferably 8, and the liquid preferably contains EDTA. It is also preferred that before the heating, the acid-fast bacteria be treated with lipase.
摘要翻译：一种实现耐酸菌细菌裂解的方法，包括在低于液体沸点的温度下，将含有非离子表面活性剂的液体中的耐酸细菌加热。该方法能够在短时间内以简单的方式实现耐酸菌的安全裂解，而无需使用专门的装置和试剂，能够提取基因。加热优选在96℃进行10分钟。作为非离子表面活性剂，可以使用d-山梨糖醇脂肪酸酯，聚氧乙烯二醇脱水山梨醇烷基酯，聚氧乙烯二醇对叔辛基苯基醚等。液体的pH值优选为8，液体优选含有EDTA。还优选在加热之前，用脂肪酶处理耐酸细菌。

5. 发明申请

US20060210988A1 Method for isolating nucleic acid and, for nucleic acid isolation, kit and apparatus 审中-公开
标题翻译：用于分离核酸的方法，用于核酸分离的试剂盒和装置
公开(公告)号：US20060210988A1
公开(公告)日：2006-09-21
申请号：US10553376
申请日：2004-04-22
申请人： Ken Inose , Satoshi Hashiguchi , Yuji Izumizawa
发明人： Ken Inose , Satoshi Hashiguchi , Yuji Izumizawa
IPC分类号： C12Q1/68 , C12N1/08 , C12M1/34
CPC分类号： C12N15/1003
摘要： Nucleic acids suitable for PCR amplification are isolated from a sample easily and rapidly by dissolving the sample in a buffer containing surfactant and salt, heating the obtained solution, subjecting the heated solution to gel filtration; and collecting a fraction containing nucleic acids.
摘要翻译：通过将样品溶解在含有表面活性剂和盐的缓冲液中，将所得溶液加热，使加热溶液进行凝胶过滤，从样品中分离出适合于PCR扩增的核酸; 并收集含有核酸的级分。

6. 发明申请

US20090269754A1 METHOD OF PRODUCING AMPLIFICATION PRODUCT BY PCR AND USAGE THEREOF 有权
标题翻译：通过PCR产生放大产物的方法及其用途
公开(公告)号：US20090269754A1
公开(公告)日：2009-10-29
申请号：US12294304
申请日：2007-08-07
申请人： Yuji Izumizawa , Satoshi Majima
发明人： Yuji Izumizawa , Satoshi Majima
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q2527/137 , C12Q2527/125 , C12Q2527/101
摘要： A method of producing a PCR amplification product is provided that suppresses an effect of precipitate, turbidity, or the like derived from a whole blood sample on a detection in the detection of an amplified nucleic acid by an optical unit. The amplification product complementary to a target nucleic acid in the whole blood sample is produced by PCR in a condition where a ratio of the whole blood sample in a PCR reaction solution is in the range of 0.1 to 0.9% by volume or 0.01 to 1.8 g/L in term of hemoglobin content. When the PCR is carried out with such conditions, even with an untreated whole blood sample, a monitoring of the amplification product by the optical unit can be done while suppressing the effect of the precipitate or the turbidity.
摘要翻译：提供了制备PCR扩增产物的方法，其抑制由全血样品衍生的沉淀物，浊度等对通过光学单元检测扩增的核酸的检测。在全血样品中与靶核酸互补的扩增产物通过PCR产生，其中PCR反应溶液中的全血样品的比例为0.1至0.9体积％或0.01至1.8g / L在血红蛋白含量方面。当用这种条件进行PCR时，即使使用未处理的全血样品，也可以通过光学单元监测扩增产物，同时抑制沉淀物的影响或浊度。

7. 发明申请

US20080277348A1 Liquid Exchange Method, Ingredient Extraction Method Using the Same, Composite Container and Autoanalyzer 审中-公开
标题翻译：液体交换方法，使用它的成分萃取方法，复合容器和自动分析仪
公开(公告)号：US20080277348A1
公开(公告)日：2008-11-13
申请号：US11886188
申请日：2006-05-22
申请人： Yuji Izumizawa
发明人： Yuji Izumizawa
IPC分类号： B03C1/02 , G01N33/20
CPC分类号： B03C1/286 , B01L3/502 , B01L2200/0647 , B01L2300/0803 , B01L2300/0809 , B01L2300/087 , B01L2400/043 , B03C1/284 , B03C2201/18 , G01N33/54333 , G01N35/0098
摘要： A liquid exchange method of exchanging liquids present around a magnetic substance is provided. The method serves to prevent a remaining liquid from transferring to a next step and a loss of the magnetic substance. Even when the liquid exchange method is applied to an autoanalyzer, the device structure will not be complicated. A vessel 11 in which a liquid 16 containing a magnetic substance 15, a vessel 12 containing a liquid 17, a magnetic substance move-mediating member 13 and also a magnet 18 are prepared. The magnet 18 is disposed at the exterior of the vessel 11, and the magnet 18 is moved to move the magnetic substance 15 on the inner wall face of the vessel 11 in order to take out the magnetic substance 15 from the magnetic substance containing liquid 16, to move the magnetic substance 15 from the inner wall face of the vessel 11 to the surface of the magnetic substance move-mediating member 13, and to move to the inner wall face of the other vessel 12 via the magnetic substance move-mediating member 13 so as to introduce the magnetic substance 15 into the liquid 17 in the vessel 12, thereby exchanging the liquids present around the magnetic substance 15.
摘要翻译：提供了一种交换存在于磁性物质周围的液体的液体交换方法。该方法用于防止剩余液体转移到下一步骤和磁性物质的损失。即使将液体交换方法应用于自动分析仪，装置结构也不复杂。制备容器11，其中容纳有磁性物质15的液体16，容纳液体17的容器12，磁性物质移动介质构件13和磁体18。磁体18设置在容器11的外部，磁体18被移动以将磁性物质15移动到容器11的内壁面上，以便从含磁性物质的液体16中取出磁性物质15 将磁性物质15从容器11的内壁面移动到磁性物质移动介质构件13的表面，并且经由磁性物质移动介质构件移动到另一容器12的内壁面以便将磁性物质15引入容器12中的液体17中，从而交换存在于磁性物质15周围的液体。

8. 发明授权

US08148079B2 Method of producing amplification product by PCR and usage thereof 有权
标题翻译：通过PCR产生扩增产物的方法及其用途
公开(公告)号：US08148079B2
公开(公告)日：2012-04-03
申请号：US12294304
申请日：2007-08-07
申请人： Yuji Izumizawa , Satoshi Majima
发明人： Yuji Izumizawa , Satoshi Majima
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q2527/137 , C12Q2527/125 , C12Q2527/101
摘要： A method of producing a PCR amplification product is provided that suppresses an effect of precipitate, turbidity, or the like derived from a whole blood sample on a detection in the detection of an amplified nucleic acid by an optical unit. The amplification product complementary to a target nucleic acid in the whole blood sample is produced by PCR in a condition where a ratio of the whole blood sample in a PCR reaction solution is in the range of 0.1 to 0.9% by volume or 0.01 to 1.8 g/L in term of hemoglobin content. When the PCR is carried out with such conditions, even with an untreated whole blood sample, a monitoring of the amplification product by the optical unit can be done while suppressing the effect of the precipitate or the turbidity.
摘要翻译：提供了制备PCR扩增产物的方法，其抑制由全血样品衍生的沉淀物，浊度等对通过光学单元检测扩增的核酸的检测。在全血样品中与靶核酸互补的扩增产物通过PCR产生，其中PCR反应溶液中的全血样品的比例为0.1至0.9体积％或0.01至1.8g / L在血红蛋白含量方面。当用这种条件进行PCR时，即使使用未处理的全血样品，也可以通过光学单元监测扩增产物，同时抑制沉淀物的影响或浊度。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式