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    • 7. 发明授权
    • Primary cultured adipocytes for gene therapy
    • 原代培养的脂肪细胞进行基因治疗
    • US08071085B2
    • 2011-12-06
    • US12175186
    • 2008-07-17
    • Masashi ItoYasushi Saito
    • Masashi ItoYasushi Saito
    • A61K48/00C12P21/00C12P21/02
    • C12N5/0653A61K35/12C12N2510/00C12N2510/02
    • The present invention relates to primary cultured adipocytes for gene therapy, where the adipocytes stably maintain a foreign gene encoding a protein that is secreted outside of cells. This invention provides cells suitable for gene therapy, which can replace bone marrow cells and liver cells used for conventional ex vivo gene therapy. The present invention established methods for transferring foreign genes into primary cultured adipocytes, which are suitable for ex vivo gene therapy; can be easily collected and implanted; and can be removed after implantation. Specifically, the present invention established these methods that use retroviral vectors. The present invention also established primary cultured adipocytes for gene therapy, where the adipocytes stably maintain a foreign gene encoding a protein that is secreted outside of cells.
    • 本发明涉及用于基因治疗的原代培养的脂肪细胞,其中脂肪细胞稳定地维持编码在细胞外分泌的蛋白质的外源基因。 本发明提供适用于基因治疗的细胞,其可代替用于常规体外基因治疗的骨髓细胞和肝细胞。 本发明建立了将外源基因转移到适合离体基因治疗的原代培养脂肪细胞中的方法; 可以轻松收集和植入; 并且可以在植入后去除。 具体地,本发明建立了使用逆转录病毒载体的这些方法。 本发明还建立了用于基因治疗的原代培养的脂肪细胞,其中脂肪细胞稳定地保持编码在细胞外分泌的蛋白质的外源基因。
    • 9. 发明申请
    • Method for Manufacturing Optically Active Tetrahydrothiophene Derivative and Method for Crystallization of Optically Active Tetrahydrothiophene-3-Ol
    • 光学活性四氢噻吩衍生物的制备方法和光活性四氢噻吩-3-O的结晶方法
    • US20080050787A1
    • 2008-02-28
    • US10581366
    • 2004-12-01
    • Hazuki NagaiKaname KonukiMasashi ItoTomohiro Sameshima
    • Hazuki NagaiKaname KonukiMasashi ItoTomohiro Sameshima
    • C12P17/00C07D333/32
    • C12P17/167
    • A method for manufacturing (R)-tetrahydrothiophene-3-ol denoted by formula (II): by bioconversion of tetrahydrothiophene-3-one denoted by formula (I): to (R)-tetrahydrothiophene-3-ol denoted by formula (II). It comprises the steps of: (A) incubating the tetrahydrothiophene-3-one denoted by formula (I) in the presence of a strain, or a preparation of a cultured cell thereof, belonging to Penicillium, Aspergillus, or Streptomyces that is capable of said bioconversion; and (B) collecting the (R)-tetrahydrothiophene-3-ol denoted by formula (II) from incubated solution. A method for crystallization of optically active tetrahydrothiophene-3-ol of improved optical purity, characterized by maintaining a solution comprising optically active tetrahydrothiophene-3-ol and organic solvent at equal to or lower than 1° C. to cause optically active tetrahydrothiophene-3-ol to crystallize from said solution, or characterized by adding optically active tetrahydrothiophene-3-ol dropwise to organic solvent at a solution temperature of equal to or lower than 1° C. to cause optically active tetrahydro thiophene-3-ol to crystallize.
    • 由式(II)表示的(R) - 四氢噻吩-3-醇的制造方法:通过式(I)表示的四氢噻吩-3-酮的生物转化:式(II)表示的(R) - 四氢噻吩-3-醇 )。 其包括以下步骤:(A)在菌株存在下将式(I)表示的四氢噻吩-3-酮或其培养细胞的制剂,属于青霉属,曲霉属或链霉菌属,其能够 说生物转化 和(B)从温育溶液中收集由式(II)表示的(R) - 四氢噻吩-3-醇。 一种提高光学纯度的光学活性四氢噻吩-3-醇的结晶方法,其特征在于将含有光学活性四氢噻吩-3-醇和有机溶剂的溶液保持在等于或低于1℃,使光学活性的四氢噻吩-3 - 醇从所述溶液中结晶,或者特征在于在等于或低于1℃的溶液温度下将光学活性的四氢噻吩-3-醇滴加到有机溶剂中,使光学活性的四氢噻吩-3-醇结晶。
    • 10. 发明申请
    • Primarily cultured adipocytes for gene therapy
    • 主要培养的脂肪细胞进行基因治疗
    • US20060121008A1
    • 2006-06-08
    • US10518472
    • 2003-06-18
    • Masashi ItoYasushi Saito
    • Masashi ItoYasushi Saito
    • A61K48/00C12N5/08C12N15/867C12N15/861
    • C12N5/0653A61K35/12C12N2510/00C12N2510/02
    • The present invention relates to primary cultured adipocytes for gene therapy, where the adipocytes stably maintain a foreign gene encoding a protein that is secreted outside of cells. This invention provides cells suitable for gene therapy, which can replace bone marrow cells and liver cells used for conventional ex vivo gene therapy. The present invention established methods for transferring foreign genes into primary cultured adipocytes, which are suitable for ex vivo gene therapy; can be easily collected and implanted; and can be removed after implantation. Specifically, the present invention established these methods that use retroviral vectors. The present invention also established primary cultured adipocytes for gene therapy, where the adipocytes stably maintain a foreign gene encoding a protein that is secreted outside of cells.
    • 本发明涉及用于基因治疗的原代培养的脂肪细胞,其中脂肪细胞稳定地维持编码在细胞外分泌的蛋白质的外源基因。 本发明提供适用于基因治疗的细胞,其可代替用于常规体外基因治疗的骨髓细胞和肝细胞。 本发明建立了将外源基因转移到适合离体基因治疗的原代培养脂肪细胞中的方法; 可以轻松收集和植入; 并且可以在植入后去除。 具体地,本发明建立了使用逆转录病毒载体的这些方法。 本发明还建立了用于基因治疗的原代培养的脂肪细胞,其中脂肪细胞稳定地保持编码在细胞外分泌的蛋白质的外源基因。