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    • 2. 发明授权
    • Method of filling liquid sample
    • 液体样品的填充方法
    • US08535620B2
    • 2013-09-17
    • US12963684
    • 2010-12-09
    • Fumio Takagi
    • Fumio Takagi
    • B01L3/00G01N35/00G01N1/10C12M1/36C12M1/22
    • B65B3/04B01L3/0293B01L3/502707B01L3/50273B01L3/505B01L3/527B01L2200/12B01L2300/123B01L2400/0481Y10T436/11Y10T436/2575
    • A method of filling a liquid sample includes: supplying the liquid sample to a first well of a biochip; adhering a cover and the substrate of the biochip in a loop-shaped area surrounding the first well and plural second wells on the substrate; moving the liquid sample from the first well to the second wells through a space between the cover and the substrate by rotating the biochip around a rotation axis in a state in which the biochip is arranged such that a distance from any one of the second wells to the rotation axis in a vertical direction with respect to the rotation axis is longer than a distance from the first well to the rotation axis in the vertical direction with respect to the rotation axis; and sealing the first well and the second wells by adhering the cover to the substrate to thereby seal.
    • 一种填充液体样品的方法包括:将液体样品供应到生物芯片的第一孔; 将覆盖物和生物芯片的基板粘附在围绕第一阱的环形区域和基板上的多个第二阱; 通过在生物芯片布置的状态下围绕旋转轴转动生物芯片,使液体样品从第一孔到第二孔通过盖和衬底之间的空间移动,使得从第二阱中的任何一个到第 相对于旋转轴在垂直方向上的旋转轴比相对于旋转轴在垂直方向上从第一孔到旋转轴的距离长; 并且通过将盖子粘附到基底上来密封第一孔和第二孔,从而密封。
    • 9. 发明授权
    • Thermal cycler and thermal cycling method
    • 热循环仪和热循环法
    • US08592160B2
    • 2013-11-26
    • US13297747
    • 2011-11-16
    • Yuji SaitoFumio Takagi
    • Yuji SaitoFumio Takagi
    • C12Q1/68C12P19/34
    • B01L7/52B01L3/5082B01L7/525B01L2300/1872B01L2400/0409B01L2400/0457
    • A thermal cycler includes a holder to which a biotip having a longitudinal direction is attached in such a manner that one end portion of the biotip is at a higher level than the other end portion, and that the distance between one end portion of the biotip and the rotational axis is shorter than the distance between the other end portion of the biotip and the rotational axis, a heating unit heats a first end portion of the biotip, a rotating unit rotates the holder, and a controller that controls the rotation speed of the rotating unit. The controller has a first mode a rotation speed at which the magnitude of the centrifugal force acting on the reaction mixture becomes smaller than the gravity, and a second mode a rotation speed at which the magnitude of the centrifugal force acting on the reaction mixture becomes greater than the gravity.
    • 热循环仪包括保持器,具有长度方向的生物体以这样的方式被附着,使得生物体的一个端部处于比另一个端部更高的水平,并且生物体的一个端部之间的距离和 旋转轴短于生物体的另一端部与旋转轴的距离,加热单元加热生物体的第一端部,旋转单元使保持器旋转,控制器控制旋转轴的旋转速度 旋转单元。 控制器具有第一模式,作用在反应混合物上的离心力的大小变得小于重力的旋转速度,第二模式是作用在反应混合物上的离心力的大小变大的第二模式 比重力。