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1. 发明申请

US20140186827A1 ASSAYS FOR THE DETECTION OF GENOTYPE, MUTATIONS, AND/OR ANEUPLOIDY 审中-公开
标题翻译：用于检测基因组，突变和/或异常的测定
公开(公告)号：US20140186827A1
公开(公告)日：2014-07-03
申请号：US13977629
申请日：2011-05-16
申请人： Martin Pieprzyk , Robert C. Jones , Kenneth J. Livak , Andrew May , Alain Mir , Jian Qin , Ramesh Ramakrishnan , Sandra Spurgeon , Jun Wang , Bernhard G. Zimmermann
发明人： Martin Pieprzyk , Robert C. Jones , Kenneth J. Livak , Andrew May , Alain Mir , Jian Qin , Ramesh Ramakrishnan , Sandra Spurgeon , Jun Wang , Bernhard G. Zimmermann
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , G01N33/542 , G01N33/582 , G01N33/6893 , G01N2800/368 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2535/131 , C12Q2537/143 , C12Q2537/16 , C12Q2563/173
摘要： The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.
摘要翻译：本发明提供用于检测基因型，突变和/或非整倍体的基于扩增的方法。这些方法具有广泛的适用性，但是特别适合于检测和定量存在于母体体液样品中的游离胎儿DNA中的靶核酸。

2. 发明申请

US20120115143A1 Universal Probe Assay Methods 有权
标题翻译：通用探针测定方法
公开(公告)号：US20120115143A1
公开(公告)日：2012-05-10
申请号：US13280214
申请日：2011-10-24
申请人： Kenneth J. Livak , Jason A. A. West , Robert C. Jones
发明人： Kenneth J. Livak , Jason A. A. West , Robert C. Jones
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6876 , C12Q2600/178 , C12Q2525/155 , C12Q2525/307 , C12Q2537/161
摘要： Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.
摘要翻译：提供了用于检测样品中靶多核苷酸的存在的试剂和方法。一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并将第一检测探针与扩增产物杂交，所述第一检测探针包括与第一探针结合序列杂交的第一片段和与第二探针结合序列杂交的第二片段，从而产生标记的扩增产物。

3. 发明授权

US09353406B2 Universal probe assay methods 有权
标题翻译：通用探针测定方法
公开(公告)号：US09353406B2
公开(公告)日：2016-05-31
申请号：US13280214
申请日：2011-10-24
申请人： Kenneth J. Livak , Jason A. A. West , Robert C. Jones
发明人： Kenneth J. Livak , Jason A. A. West , Robert C. Jones
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6876 , C12Q2600/178 , C12Q2525/155 , C12Q2525/307 , C12Q2537/161
摘要： Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.
摘要翻译：提供了用于检测样品中靶多核苷酸的存在的试剂和方法。一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并且将第一检测探针与扩增产物杂交，所述第一检测探针包含与第一探针结合序列杂交的第一区段和与第二探针结合序列杂交的第二区段，从而产生标记的扩增产物。

4. 发明申请

US20120172252A1 High Density Sequence Detection Methods 审中-公开
标题翻译：高密度序列检测方法
公开(公告)号：US20120172252A1
公开(公告)日：2012-07-05
申请号：US13420998
申请日：2012-03-15
申请人： Timothy M. Woudenberg , Robert C. Jones , Kenneth J. Livak
发明人： Timothy M. Woudenberg , Robert C. Jones , Kenneth J. Livak
IPC分类号： C40B30/04 , C40B40/06
CPC分类号： B01L3/50851 , B01L3/50853 , B01L3/50857 , B01L2200/0642 , B01L2300/044 , B01L2300/0819 , B01L2300/0829 , B01L2400/0409 , B01L2400/0487 , C12Q1/6837
摘要： A method for performing PCR on a liquid sample comprising a plurality of polynucleotide targets, wherein each polynucleotide target is present at very low concentration within the sample. The method comprises applying PCR reactants to the surface of a substrate to produce a plurality of reaction spots on the surface of the substrate; loading the liquid sample and a PCR reagent mixture onto the reaction spots; forming a sealed reaction chamber, having a volume of less than about 20 nanoliters, over each of the reaction spots; and amplifying the sample.
摘要翻译：在包含多个多核苷酸靶标的液体样品上进行PCR的方法，其中每个多核苷酸靶标以非常低的浓度存在于样品内。该方法包括将PCR反应物施加到基底的表面以在基底的表面上产生多个反应点; 将液体样品和PCR试剂混合物加载到反应点上; 在每个反应点上形成体积小于约20纳升的密封反应室; 并放大样品。

5. 发明申请

US20100173293A1 High Density Sequence Detection Methods 审中-公开
标题翻译：高密度序列检测方法
公开(公告)号：US20100173293A1
公开(公告)日：2010-07-08
申请号：US12497398
申请日：2009-07-02
申请人： Timothy M. Woudenberg , Robert C. Jones , Kenneth J. Livak
发明人： Timothy M. Woudenberg , Robert C. Jones , Kenneth J. Livak
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： B01L3/50851 , B01L3/50853 , B01L3/50857 , B01L2200/0642 , B01L2300/044 , B01L2300/0819 , B01L2300/0829 , B01L2400/0409 , B01L2400/0487 , C12Q1/6837
摘要： A method for performing PCR on a liquid sample comprising a plurality of polynucleotide targets, wherein each polynucleotide target is present at very low concentration within the sample. The method comprises applying PCR reactants to the surface of a substrate to produce a plurality of reaction spots on the surface of the substrate; loading the liquid sample and a PCR reagent mixture onto the reaction spots; forming a sealed reaction chamber, having a volume of less than about 20 nanoliters, over each of the reaction spots; and amplifying the sample.
摘要翻译：在包含多个多核苷酸靶标的液体样品上进行PCR的方法，其中每个多核苷酸靶标以非常低的浓度存在于样品内。该方法包括将PCR反应物施加到基底的表面以在基底的表面上产生多个反应点; 将液体样品和PCR试剂混合物加载到反应点上; 在每个反应点上形成体积小于约20纳升的密封反应室; 并放大样品。

6. 发明申请

US20130045881A1 Probe Based Nucleic Acid Detection 有权
标题翻译：基于探针的核酸检测
公开(公告)号：US20130045881A1
公开(公告)日：2013-02-21
申请号：US13467933
申请日：2012-05-09
申请人： Kenneth J. Livak , Stacey N. Myers , Jun Wang , Xiaohui Wang
发明人： Kenneth J. Livak , Stacey N. Myers , Jun Wang , Xiaohui Wang
IPC分类号： G01N21/64 , C40B30/04
CPC分类号： C12Q1/6818 , C12Q2525/151
摘要： The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.
摘要翻译：本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

7. 发明授权

US08809238B2 Probe based nucleic acid detection 有权
标题翻译：基于探针的核酸检测
公开(公告)号：US08809238B2
公开(公告)日：2014-08-19
申请号：US13467933
申请日：2012-05-09
申请人： Kenneth J. Livak , Stacey N. Meyers , Jun Wang , Xiaohui Wang
发明人： Kenneth J. Livak , Stacey N. Meyers , Jun Wang , Xiaohui Wang
IPC分类号： G01N21/64 , C40B30/04 , C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2525/151
摘要： The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.
摘要翻译：本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

8. 发明申请

US20130022973A1 Multiplex Amplification for the Detection of Nucleic Acid Variations 审中-公开
标题翻译：用于检测核酸变异的多重扩增
公开(公告)号：US20130022973A1
公开(公告)日：2013-01-24
申请号：US13521400
申请日：2011-01-14
申请人： Carl L. G. Hansen , Oleh Petriv , Kevin Heyries , Kenneth J. Livak
发明人： Carl L. G. Hansen , Oleh Petriv , Kevin Heyries , Kenneth J. Livak
IPC分类号： C12Q1/68 , G01N21/64
CPC分类号： C12Q1/6851 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2537/143 , C12Q2537/165 , C12Q2563/159
摘要： Kits, primers, and methods are provided herein for detecting relative target source to reference source ratios in a biological sample, by distributing the biological sample into discrete subsamples, wherein the biological sample includes, a plurality of target molecules on a target source; and a plurality of reference molecules on a reference source; providing target primers directed to one or more of the plurality of target molecules and reference primers directed to one or more of the plurality of reference molecules; performing digital amplification with the target primers and the reference primers; and detecting the presence or absence of amplified target products with target probes and detecting the presence or absence of amplified reference products with reference probes, wherein the ratio of amplified target products to amplified reference products is indicative of a relative amount of target source to reference source in a biological sample.
摘要翻译：本文提供了试剂盒，引物和方法，用于通过将生物样品分配到离散的子样品中来检测生物样品中的相对目标来源参考源比例，其中生物样品包括目标源上的多个靶分子; 和参考源上的多个参考分子; 提供指向所述多个靶分子中的一个或多个的靶引物和指向所述多个参考分子中的一个或多个的参考引物; 用目标引物和参照引物进行数字扩增; 并用靶探针检测扩增的靶产物的存在或不存在，并用参考探针检测扩增的参考产物的存在或不存在，其中扩增的靶产物与扩增的参考产物的比率指示靶源与参考源的相对量在生物样品中。

9. 发明申请

US20110251083A1 Multiplexed Amplification of Short Nucleic Acids 审中-公开
标题翻译：短核酸的多重扩增
公开(公告)号：US20110251083A1
公开(公告)日：2011-10-13
申请号：US12762272
申请日：2010-04-16
申请人： Kai Qin LAO , Kenneth J. Livak , Neil A. Straus
发明人： Kai Qin LAO , Kenneth J. Livak , Neil A. Straus
IPC分类号： C40B30/04 , C12P19/34 , C40B40/06 , C12Q1/68
CPC分类号： C12N15/1065 , C12Q1/6851 , C12Q1/686 , C12Q2525/301 , C12Q2525/161 , C12Q2563/179 , C12Q2537/143 , C12Q2525/207
摘要： The present teachings provide methods, compositions, and kits for reverse transcribing and amplifying small nucleic acids such as micro RNAs. High levels of multiplexing are provided by the use of a zip-coded stem-loop reverse transcription primer, along with a PCR-based pre-amplification reaction that comprises a zip-coded forward primer. Detector probes in downstream decoding PCRs can take advantage of the zip-code introduced by the stem-loop reverse transcription primer. In some embodiments, further amplification is achieved by cycling the reverse transcription reaction. The present teachings also provide compositions and kits useful for performing the reverse transcription and amplification reactions described herein.
摘要翻译：本教导提供用于逆转录和扩增小核酸如微RNA的方法，组合物和试剂盒。通过使用zip编码的茎 - 环逆转录引物以及包含经翻译的正向引物的基于PCR的预扩增反应来提供高水平的复用。下游解码PCR中的检测器探针可以利用茎环逆转录引物引入的zip码。在一些实施方案中，通过循环逆转录反应来实现进一步的扩增。本教导还提供可用于进行本文所述的逆转录和扩增反应的组合物和试剂盒。

10. 发明申请

US20090239290A1 SINGLE-TUBE, READY-TO-USE ASSAY KITS, AND METHODS USING SAME 审中-公开
标题翻译：单管，即用型测试工具，以及使用相同的方法
公开(公告)号：US20090239290A1
公开(公告)日：2009-09-24
申请号：US12404674
申请日：2009-03-16
申请人： Michael W. Hunkapiller , Dennis A. Gilbert , Kenneth J. Livak , Junko Stevens , Susan K. Eddins , Michael Lucero
发明人： Michael W. Hunkapiller , Dennis A. Gilbert , Kenneth J. Livak , Junko Stevens , Susan K. Eddins , Michael Lucero
IPC分类号： C12M1/00
CPC分类号： C12Q1/6858 , G16B50/00 , C12Q2547/101
摘要： An assay kit is provided that includes assay reagents stored in a single-tube container, and a data storage medium containing information about the contents of the container. Methods are provided for using the data provided with the kit to direct instruments and/or processes, for example, to control an instrument to perform amplification and/or sequencing reactions.
摘要翻译：提供了包含存储在单管容器中的测定试剂和含有关于容器内容物的信息的数据存储介质的测定试剂盒。提供了使用试剂盒提供的数据来指导仪器和/或过程的方法，例如控制仪器进行扩增和/或测序反应。

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