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1. 发明申请

US20060010513A1 Oligonucleotide arrays to monitor gene expression and methods for making and using same 审中-公开
标题翻译：用于监测基因表达的寡核苷酸阵列及其制备和使用方法
公开(公告)号：US20060010513A1
公开(公告)日：2006-01-12
申请号：US11128049
申请日：2005-05-11
申请人： Mark Melville , Timothy Charlebois , William Mounts , Louane Hann , Martin Sinacore , Mark Leonard , Eugene Brown , Christopher Miller , Gene Lee
发明人： Mark Melville , Timothy Charlebois , William Mounts , Louane Hann , Martin Sinacore , Mark Leonard , Eugene Brown , Christopher Miller , Gene Lee
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C07K14/47 , C12Q1/6837 , C12Q1/6876 , C12Q2600/158
摘要： The present invention provides an oligonucleotide array capable of identifying genes and related pathways involved with the induction of a particular phenotype by a cell line, e.g., the genes and related pathways involved with the induction of transgene expression by the cell line. The invention is particularly useful when there is little or no information about the genome of the cell line being studied, because it provides methods for identifying consensus sequences for known and previously undiscovered genes, and for designing oligonucleotide probes to the identified consensus sequences. Additionally, when the array is to be used to determine optimal conditions for expression of a transgene by the cell line, the invention teaches methods of including oligonucleotide probes to transgene sequences in the array. The invention also provides methods of using the array to identify genes and related pathways involved with the induction of a particular cell line phenotype. The invention also provides novel polynucleotides of undiscovered genes (i.e., a gene that had not been sequenced and/or shown to be expressed by CHO cells) and novel polynucleotides involved with the induction of a particular cell phenotype, e.g., increased survival when grown under stressful culture conditions, increased transgene expression, decreased production of an antigen, etc. These novel polynucleotides are termed novel CHO sequences and differential CHO sequences, respectively. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention.
摘要翻译：本发明提供了能够鉴定涉及通过细胞系诱导特定表型的基因和相关途径的寡核苷酸阵列，例如涉及通过细胞系诱导转基因表达的基因和相关途径。当关于正在研究的细胞系的基因组的信息很少或没有信息时，本发明是特别有用的，因为它提供了用于鉴定已知和先前未被发现的基因的共有序列的方法，以及为所识别的共有序列设计寡核苷酸探针。另外，当该阵列用于确定由细胞系表达转基因的最佳条件时，本发明教导了将阵列中的转基因序列包含寡核苷酸探针的方法。本发明还提供了使用该阵列鉴定涉及诱导特定细胞系表型的基因和相关途径的方法。本发明还提供未被发现的基因的新型多核苷酸（即，未被测序和/或显示由CHO细胞表达的基因）和参与特定细胞表型诱导的新型多核苷酸，例如当在下面生长时增加的存活压力培养条件，转基因表达增加，抗原产生减少等。这些新型多核苷酸分别被称为新的CHO序列和差异CHO序列。本发明还提供了遗传工程改造的表达载体，宿主细胞和包含本发明的新型核酸分子的转基因动物。本发明还向本发明的核酸分子提供反义和RNAi分子。本发明还提供使用本发明的多核苷酸的方法。

2. 发明申请

US20060003958A1 Novel polynucleotides related to oligonucleotide arrays to monitor gene expression 审中-公开
标题翻译：与寡核苷酸阵列相关的新型多核苷酸以监测基因表达
公开(公告)号：US20060003958A1
公开(公告)日：2006-01-05
申请号：US11128061
申请日：2005-05-11
申请人： Mark Melville , Timothy Charlebois , William Mounts , Louane Hann , Martin Sinacore , Mark Leonard , Eugene Brown , Christopher Miller , Gene Lee
发明人： Mark Melville , Timothy Charlebois , William Mounts , Louane Hann , Martin Sinacore , Mark Leonard , Eugene Brown , Christopher Miller , Gene Lee
IPC分类号： A61K48/00 , C07K14/47 , C12Q1/68 , C07H21/04 , C12N5/06
CPC分类号： C07K14/47 , C12Q1/6837 , C12Q1/6876 , C12Q2600/158
摘要： The present invention provides an oligonucleotide array capable of identifying genes and related pathways involved with the induction of a particular phenotype by a cell line, e.g., the genes and related pathways involved with the induction of transgene expression by the cell line. The invention is particularly useful when there is little or no information about the genome of the cell line being studied, because it provides methods for identifying consensus sequences for known and previously undiscovered genes, and for designing oligonucleotide probes to the identified consensus sequences. Additionally, when the array is to be used to determine optimal conditions for expression of a transgene by the cell line, the invention teaches methods of including oligonucleotide probes to transgene sequences in the array. The invention also provides methods of using the array to identify genes and related pathways involved with the induction of a particular cell line phenotype. The invention also provides novel polynucleotides of undiscovered genes (i.e., a gene that had not been sequenced and/or shown to be expressed by CHO cells) and novel polynucleotides involved with the induction of a particular cell phenotype, e.g., increased survival when grown under stressful culture conditions, increased transgene expression, decreased production of an antigen, etc. These novel polynucleotides are termed novel CHO sequences and differential CHO sequences, respectively. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention.
摘要翻译：本发明提供了能够鉴定涉及通过细胞系诱导特定表型的基因和相关途径的寡核苷酸阵列，例如涉及通过细胞系诱导转基因表达的基因和相关途径。当关于正在研究的细胞系的基因组的信息很少或没有信息时，本发明是特别有用的，因为它提供了用于鉴定已知和先前未被发现的基因的共有序列的方法，以及为所识别的共有序列设计寡核苷酸探针。另外，当该阵列用于确定由细胞系表达转基因的最佳条件时，本发明教导了将阵列中的转基因序列包含寡核苷酸探针的方法。本发明还提供了使用该阵列鉴定涉及诱导特定细胞系表型的基因和相关途径的方法。本发明还提供未被发现的基因的新型多核苷酸（即，未被测序和/或显示由CHO细胞表达的基因）和参与特定细胞表型诱导的新型多核苷酸，例如当在下面生长时增加的存活压力培养条件，转基因表达增加，抗原产生减少等。这些新型多核苷酸分别被称为新的CHO序列和差异CHO序列。本发明还提供了遗传工程改造的表达载体，宿主细胞和包含本发明的新型核酸分子的转基因动物。本发明还向本发明的核酸分子提供反义和RNAi分子。本发明还提供使用本发明的多核苷酸的方法。

3. 发明申请

US20070270331A1 Modulators of gluconeogenesis 失效
标题翻译：糖异生的调节剂
公开(公告)号：US20070270331A1
公开(公告)日：2007-11-22
申请号：US11717426
申请日：2007-03-13
申请人： Stephen Berasi , Christine Huard , Dongmei Li , Heather Shih , Ying Sun , Janet Paulsen , Eugene Brown , Ruth Gimeno , Robert Martinez
发明人： Stephen Berasi , Christine Huard , Dongmei Li , Heather Shih , Ying Sun , Janet Paulsen , Eugene Brown , Ruth Gimeno , Robert Martinez
IPC分类号： A61K38/02 , A61K31/7052 , A61P3/08 , C12N5/02
CPC分类号： C07K14/4702 , A61K38/1709 , C12N9/16
摘要： The present invention provides methods of affecting gluconeogenesis by altering the levels or activity of DUSP4 and/or EGR1. Pharmaceutical compositions comprising or encoding modulators of gluconeogenesis are also disclosed.
摘要翻译：本发明提供通过改变DUSP4和/或EGR1的水平或活性来影响糖异生的方法。还公开了包含或编码糖异生的调节剂的药物组合物。

4. 发明申请

US20070051559A1 Maintenance stand 有权
标题翻译：维护架
公开(公告)号：US20070051559A1
公开(公告)日：2007-03-08
申请号：US11220125
申请日：2005-09-06
申请人： Eugene Brown , Eugene Nelson
发明人： Eugene Brown , Eugene Nelson
IPC分类号： E04C1/00
CPC分类号： B66F11/042
摘要： A wheeled maintenance stand for aircraft has a worker platform elevated by a pair of double scissor linkages, which have track and follower connections with a mobile base frame and the worker platform. A staircase with self-leveling steps is connected to a laterally outer side of one of the double scissor linkages. A power unit supplies fluid power for propelling the stand, operating the scissor linkages and steering. The upper worker platform has left, right and center rolling sections. A manually operated brake is provides for each of the rolling sections. Electric power, compressed air and cooling air are supplied to the upper work platform
摘要翻译：用于飞机的轮式维护支架具有由一对双剪刀连接器提升的工作平台，其具有与移动基座和工作平台的跟踪和跟随连接。具有自流平台阶的楼梯连接到双剪刀连杆之一的横向外侧。动力单元提供流体动力来推动支架，操作剪刀连杆和转向。上层工作平台有左，右和中心滚动部分。为每个轧制部分提供手动制动器。电力，压缩空气和冷却空气供应到上部工作平台

5. 发明申请

US20060164447A1 Ethernet and USB powered printers and methods for supplying ethernet and USB power to a printer 有权
公开(公告)号：US20060164447A1
公开(公告)日：2006-07-27
申请号：US11336089
申请日：2006-01-20
申请人： David Poole , Douglas Fox , Robert Ehrhardt , Eugene Brown , Rod DeMay , John Ham , Gary Lynch , Donald Thielen
发明人： David Poole , Douglas Fox , Robert Ehrhardt , Eugene Brown , Rod DeMay , John Ham , Gary Lynch , Donald Thielen
IPC分类号： B41J29/38
CPC分类号： G06F3/1221 , B41J29/393 , G06F3/1215 , G06F3/1229 , G06F3/1286 , G06K15/4055
摘要： Ethernet and Universal Serial Bus (USB) powered printing devices are provided. In particular, Ethernet and USB powered printers and methods of Ethernet powering of printers that support high speed printing and/or data intensive printing. Such high-speed and/or data intensive printing operations are able to be Ethernet or USB powered by providing for a power storage unit within the printer device that is able be charged during non-printing periods and provide for necessary energy bursts to support such higher powered processes. In addition, the present invention provides for devices and methods that allow for printers to operate with main power provided by conventional power mains and for back-up or secondary power to be supplied by Ethernet or USB power. Additionally, the invention provides for Ethernet or USB powering, both primary and back-up, of the data and configuration settings in the printer control and image generating electronics. In the back-up power mode, the Ethernet or USB power insures that data and configurations are not lost during periods of main power outage. The invention also provides a printer and method that can optimize a printing operation, e.g., by adjusting the speed of the printing operation according to the power required for the operation and/or the power available.

6. 发明申请

US20050014142A1 Hybridization normalization methods 审中-公开
标题翻译：杂交归一化方法
公开(公告)号：US20050014142A1
公开(公告)日：2005-01-20
申请号：US10479866
申请日：2002-06-06
申请人： Uwe Scherf , Michael Elashoff , Yasmin Beazer-Barclay , Kristen Antonellis , Scott Jelinsky , Maryann Whitley , Eugene Brown
发明人： Uwe Scherf , Michael Elashoff , Yasmin Beazer-Barclay , Kristen Antonellis , Scott Jelinsky , Maryann Whitley , Eugene Brown
IPC分类号： C12Q1/68 , C12Q1/6832 , C12Q1/6837
CPC分类号： C12Q1/6832 , C12Q1/6837 , C12Q2545/113
摘要： The present invention includes methods of normalizing hybridization reactions that are designed to select normalization control genes, specifically 5′-3′-, and middle portions of the these genes, that hybridize similarly to a probe array and that produce the most consistently linear curve of hybridization signal over a range of normalization control gene segment concentrations. These methods have applicability across a broad spectrum of hybridization formats.
摘要翻译：本发明包括归一化杂交反应的方法，其被设计用于选择归一化对照基因，特别是这些基因的5'-3'-和中间部分，其与探针阵列类似地杂交并产生最一致的线性曲线杂交信号在一系列归一化控制基因片段浓度。这些方法在广泛的杂交格式中具有适用性。

7. 发明授权

US07257904B1 Kingpin inspection gauge and hitch adjustment tool 有权
标题翻译： Kingpin检测仪和搭接调整工具
公开(公告)号：US07257904B1
公开(公告)日：2007-08-21
申请号：US11043342
申请日：2005-01-26
申请人： Eugene Brown , Tom Moulton , Danny Henderson
发明人： Eugene Brown , Tom Moulton , Danny Henderson
IPC分类号： G01B3/14 , B25F1/00
CPC分类号： G01B5/255 , G01B2210/24
摘要： A tool for inspecting a trailer kingpin and adjusting a fifth wheel hitch includes a plate having at least two mutually substantially perpendicular sides, one side having upper and lower generally rectangular tabs extending therefrom and separated by a space therebetween, the space being dimensioned to snugly receive a kingpin lower flange, the upper tab being dimensioned to be snugly received along a kingpin shank. Opposing this tabbed side is a side having at least one shaped recess for receiving a means for adjusting play in a fifth wheel hitch and transferring the application of torque thereto. The plate also includes a shaped aperture for receiving a kingpin, the shaped aperture having a substantially rectangular wide portion and a substantially rectangular narrow portion.
摘要翻译：用于检查拖车主销并调整第五轮牵引的工具包括具有至少两个相互基本上垂直的侧面的板，一个侧面具有从其延伸的上部和下部大致矩形的突出部，并且由它们之间的空间隔开，该空间的尺寸被设计成紧贴地接收一个主销下部法兰，上部突出部的尺寸设计成沿着一个主销柄紧贴地容纳。与该标签侧相对的是具有至少一个成形凹槽的一侧，用于接收用于调整第五轮牵引中的弹奏并传递施加扭矩的装置。板还包括用于接收主销的成形孔，该成形孔具有基本上矩形的宽部分和基本上矩形的窄部分。

8. 发明申请

US20050266409A1 Compositions and methods for diagnosing, preventing, and treating cancers 审中-公开
标题翻译：用于诊断，预防和治疗癌症的组合物和方法
公开(公告)号：US20050266409A1
公开(公告)日：2005-12-01
申请号：US10770726
申请日：2004-02-04
申请人： Eugene Brown , Wei Liu
发明人： Eugene Brown , Wei Liu
IPC分类号： C12Q1/68 , G01N33/48 , G01N33/50 , G01N33/574 , G06F19/00
CPC分类号： C12N15/117 , A61K39/21 , A61K2039/53 , A61K2039/545 , A61K2039/572 , A61K2039/585 , C12N15/1058 , C12N2310/16 , C12N2310/17 , C12N2310/351 , C12N2310/531 , C12N2320/30 , C12P19/34 , C12Q1/6886 , C12Q2600/136 , C12Q2600/158 , G01N33/5308 , G01N33/57415 , G01N33/57419 , G01N33/57423 , G01N33/57434 , G01N33/6854 , G01N2333/162 , G01N2333/9121 , Y02A50/57
摘要： Compositions and methods for diagnosing, preventing, and treating cancers. In one embodiment, genes differentially expressed in colon, lung, breast and prostate cancer tissues relative to corresponding cancer-free tissues are identified. These genes or their products can be used as markers for the detection of respective cancers. Modulators of these genes or their products can be used for the treatment or prevention of respective cancers.
摘要翻译：用于诊断，预防和治疗癌症的组合物和方法。在一个实施方案中，鉴定了相对于相应的无癌组织在结肠癌，肺癌，乳腺癌和前列腺癌组织中差异表达的基因。这些基因或其产物可用作检测各自癌症的标志物。这些基因或其产物的调节剂可用于治疗或预防各自的癌症。

9. 发明申请

US20050202500A1 Expression monitoring to high density oligonucleotide arrays 审中-公开
标题翻译：对高密度寡核苷酸阵列的表达监测
公开(公告)号：US20050202500A1
公开(公告)日：2005-09-15
申请号：US11118974
申请日：2005-04-28
申请人： David Lockhart , Eugene Brown , Gordon Wong , Mark Chee , Thomas Gingeras
发明人： David Lockhart , Eugene Brown , Gordon Wong , Mark Chee , Thomas Gingeras
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N15/14 , G01N37/00 , C12P19/34 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C12Q1/6809 , C12Q1/6837 , G01N15/1475 , C12Q2565/507
摘要： This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.
摘要翻译：本发明提供监测多种基因的表达水平的方法。所述方法包括将核酸样品与寡核苷酸探针的高密度阵列杂交，其中高密度阵列含有与核酸样品中靶核酸的子序列互补的寡核苷酸探针。在一个实施方案中，所述方法包括提供包含一个或多个靶基因的RNA转录物或衍生自RNA转录物的核酸的靶核酸库，将所述核酸库与固定在表面上的寡核苷酸探针阵列杂交，其中包含超过100种不同寡核苷酸的阵列和每种不同的寡核苷酸定位在表面的预定区域中，不同寡核苷酸的密度大于每1cm 2大约60个不同的寡核苷酸，并且寡核苷酸探针与衍生自RNA转录物的RNA转录物或核酸互补; 并量化阵列中的杂交核酸。

10. 发明申请

US20050202432A1 Estrogen receptor alpha regulated gene expression, related assays and therapeutics 失效
标题翻译：雌激素受体α调控基因表达，相关测定和治疗
公开(公告)号：US20050202432A1
公开(公告)日：2005-09-15
申请号：US10511064
申请日：2003-04-10
申请人： Scott Jelinksy , Heather Harris , Eugene Brown , Mark Evans
发明人： Scott Jelinksy , Heather Harris , Eugene Brown , Mark Evans
IPC分类号： A61K48/00 , C07H21/04 , C12N15/12 , C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6876 , C12Q1/6883 , C12Q2600/158 , C12Q2565/501
摘要： A plurality of genes modulated by estrogen or other agents, such as hormones or combinations of hormones, in various types of tissue is described. One embodiment of the disclosure relates to a plurality of genes, which demonstrates certain patterns of expression differing qualitatively or quantitatively, with and without exposure to estrogen and/or other hormone compositions. Methods of using these genes in identifying candidate agents that exert at least some of the biological effects of estrogen and/or other hormone, and pharmaceuticals and related therapies also is disclosed. The use of the plurality of genes in methods of monitoring, in gene chips and in kits also is disclosed.
摘要翻译：描述了在各种类型的组织中由雌激素或其他试剂调节的多种基因，例如激素或激素组合。本公开的一个实施方案涉及多种基因，其表现出在不影响雌激素和/或其它激素组合物的情况下，定性或定量不同的某些表达模式。公开了将这些基因用于鉴定发挥雌激素和/或其他激素的至少一些生物学效应的候选药物的方法，以及药物和相关疗法。公开了在基因芯片和试剂盒中监测方法中多种基因的使用。

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