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1. 发明授权

US06653080B2 Loop probe hybridization assay for polynucleotide analysis 有权
公开(公告)号：US06653080B2
公开(公告)日：2003-11-25
申请号：US09815510
申请日：2001-03-22
申请人： Marcel P. Bruchez , Jennifer Lai , Vince Phillips , Andrew R. Watson , Edith Wong
发明人： Marcel P. Bruchez , Jennifer Lai , Vince Phillips , Andrew R. Watson , Edith Wong
IPC分类号： C12Q168
CPC分类号： B82Y5/00 , B82Y10/00 , C12Q1/6818 , C12Q1/6834 , C12Q2537/143 , C12Q2561/119 , C12Q2525/301
摘要： Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide and/or an amplification product therefrom are provided. The methods comprise contacting a sample suspected of containing the target polynucleotide with a polynucleotide that can bind specifically thereto; this polynucleotide is conjugated to a substrate, preferably an encoded bead conjugate. An amplification reaction can first be used to produce the amplification product from the target polynucleotide so that it can be used to indirectly assay for the target polynucleotide. An amplification product detection complex and method of forming the same are also provided. The methods are particularly useful in multiplex settings where a plurality of targets are present. Amplification product assay complexes and amplification product assay arrays are also provided, along with methods of forming the same. Kits comprising reagents for performing such methods are also provided.

2. 发明授权

US07129048B2 Loop probe hybridization assay for polynucleotide analysis 有权
公开(公告)号：US07129048B2
公开(公告)日：2006-10-31
申请号：US10716063
申请日：2003-11-17
申请人： Marcel P. Bruchez , Jennifer Lai , Vince Phillips , Andrew R. Watson , Edith Wong
发明人： Marcel P. Bruchez , Jennifer Lai , Vince Phillips , Andrew R. Watson , Edith Wong
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： B82Y5/00 , B82Y10/00 , C12Q1/6818 , C12Q1/6834 , C12Q2537/143 , C12Q2561/119 , C12Q2525/301
摘要： Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide and/or an amplification product therefrom are provided. The methods comprise contacting a sample suspected of containing the target polynucleotide with a polynucleotide that can bind specifically thereto; this polynucleotide is conjugated to a substrate, preferably an encoded bead conjugate. An amplification reaction can first be used to produce the amplification product from the target polynucleotide so that it can be used to indirectly assay for the target polynucleotide. An amplification product detection complex and method of forming the same are also provided. The methods are particularly useful in multiplex settings where a plurality of targets are present. Amplification product assay complexes and amplification product assay arrays are also provided, along with methods of forming the same. Kits comprising reagents for performing such methods are also provided.

3. 发明授权

US06500622B2 Methods of using semiconductor nanocrystals in bead-based nucleic acid assays 有权
标题翻译：在基于珠的核酸测定中使用半导体纳米晶体的方法
公开(公告)号：US06500622B2
公开(公告)日：2002-12-31
申请号：US09815585
申请日：2001-03-22
申请人： Marcel P. Bruchez, Jr. , Jennifer H. Lai , Vince E. Phillips , Andrew R. Watson , Edith Y. Wong
发明人： Marcel P. Bruchez, Jr. , Jennifer H. Lai , Vince E. Phillips , Andrew R. Watson , Edith Y. Wong
IPC分类号： C12Q168
CPC分类号： B82Y5/00 , B82Y10/00 , C12Q1/6818 , C12Q1/6834 , C12Q2537/143 , C12Q2561/119 , C12Q2525/301
摘要： Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide and/or an amplification product therefrom are provided. The methods comprise contacting a sample suspected of containing the target polynucleotide with a polynucleotide that can bind specifically thereto; this polynucleotide is conjugated to a substrate, preferably an encoded bead conjugate. An amplification reaction can first be used to produce the amplification product from the target polynucleotide so that it can be used to indirectly assay for the target polynucleotide. An amplification product detection complex and method of forming the same are also provided. The methods are particularly useful in multiplex settings where a plurality of targets are present. Amplification product assay complexes and amplification product assay arrays are also provided, along with methods of forming the same. Kits comprising reagents for performing such methods are also provided.
摘要翻译：提供了用于测定靶多核苷酸和/或其扩增产物的样品的方法，组合物和制品。所述方法包括使怀疑含有靶多核苷酸的样品与可特异结合的多核苷酸接触; 该多核苷酸与底物缀合，优选编码的珠缀合物。首先可以使用扩增反应来产生来自目标多核苷酸的扩增产物，使得其可以用于间接测定靶多核苷酸。还提供了扩增产物检测复合物及其形成方法。这些方法在存在多个目标的多路复用设置中特别有用。还提供了扩增产物测定复合物和扩增产物测定阵列，以及其形成方法。还提供了包含用于执行这些方法的试剂的试剂盒。

4. 发明授权

US06838243B2 Methods and compositions for polynucleotide analysis using generic capture sequences 有权
标题翻译：使用通用捕获序列的多核苷酸分析的方法和组合物
公开(公告)号：US06838243B2
公开(公告)日：2005-01-04
申请号：US09846430
申请日：2001-04-30
申请人： Jennifer H. Lai , Vincent E. Phillips , Andrew R. Watson
发明人： Jennifer H. Lai , Vincent E. Phillips , Andrew R. Watson
IPC分类号： C07H21/02 , C07H21/04 , C12M1/34 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6834 , C12Q2565/519 , C12Q2537/143 , C12Q2531/113
摘要： Methods, compositions and articles of manufacture for assaying a sample for an amplification product from a target polynucleotide are provided. An amplification reaction is used to produce the amplification product from the target polynucleotide so that it can be used to indirectly assay the sample for the target polynucleotide. A sample suspected of containing the target polynucleotide is contacted with first and second primers to amplify the target polynucleotide; the first primer comprises a tag sequence, the complement of which is formed on the opposite strand during amplification and is referred to as a capture sequence. That opposite strand is referred to as a second primer extension product or an amplification product, and comprises a label. A capture probe is provided that is conjugated to a substrate and can bind to the capture sequence to form an amplification product detection complex. Methods of detecting the amplification product thus produced are also provided, as are amplification product assay arrays, along with methods of forming the same. The methods are particularly useful in multiplex settings where a plurality of target polynucleotides are to be assayed. Kits comprising reagents for performing such methods are also provided.
摘要翻译：提供了用于从靶多核苷酸测定扩增产物样品的方法，组合物和制品。使用扩增反应从靶多核苷酸产生扩增产物，使其可用于间接测定靶多核苷酸的样品。将怀疑含有靶多核苷酸的样品与第一和第二引物接触以扩增靶多核苷酸; 第一引物包含标签序列，其互补体在扩增过程中在相对的链上形成并被称为捕获序列。该相对链被称为第二引物延伸产物或扩增产物，并且包含标记。提供了捕获探针，其与底物缀合并且可以结合捕获序列以形成扩增产物检测复合物。还提供了检测如此生产的扩增产物的方法，扩增产物测定阵列以及其形成方法。所述方法在多重设置中特别有用，其中将测定多个靶多核苷酸。还提供了包含用于执行这些方法的试剂的试剂盒。

5. 发明申请

US20100210472A1 SPATIAL POSITIONING OF SPECTRALLY LABELED BEADS 有权
标题翻译：光纤标线的空间定位
公开(公告)号：US20100210472A1
公开(公告)日：2010-08-19
申请号：US12620475
申请日：2009-11-17
申请人： Stephen A. EMPEDOCLES , Andrew R. Watson , Jian Jin
发明人： Stephen A. EMPEDOCLES , Andrew R. Watson , Jian Jin
IPC分类号： C40B30/04 , C40B30/00 , C40B60/12
CPC分类号： G01N21/272 , B01J2219/00317 , B01J2219/00432 , B01J2219/005 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00657 , B01J2219/00659 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00743 , B01L3/5025 , B01L3/502715 , B01L3/502761 , B01L3/5085 , B01L3/545 , B01L2200/0668 , B01L2300/021 , B01L2300/0819 , B01L2300/0829 , B01L2400/0454 , B01L2400/0457 , B82Y30/00 , C40B40/06 , C40B60/14 , G01N21/25 , G01N21/253 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/6489 , G01N33/54373 , G01N2015/0092 , G01N2015/1472 , G01N2015/1497 , G01N2021/6441 , G01N2021/6482 , G01N2035/00158 , G01N2035/00742 , G01N2035/00752 , G01N2201/04 , G01N2201/06113 , G01N2201/12
摘要： Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.
摘要翻译：用于检测和/或识别非常适合于进行多重测定的多个光谱标记物质的装置，系统，试剂盒和方法。通过用产生可识别光谱的材料对珠粒进行光谱标记，可以在流体内识别多个珠粒。通过将阵列中的珠抑制和/或使用聚焦激光来促进珠的读取。

6. 发明授权

US07559481B2 Differentiable spectral bar code methods and systems 有权
公开(公告)号：US07559481B2
公开(公告)日：2009-07-14
申请号：US10807616
申请日：2004-03-23
申请人： Joseph A. Treadway , Stephen A. Empedocles , Andrew R. Watson
发明人： Joseph A. Treadway , Stephen A. Empedocles , Andrew R. Watson
IPC分类号： G06K19/06
CPC分类号： G01N21/272 , B01J2219/00317 , B01J2219/00432 , B01J2219/005 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00657 , B01J2219/00659 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00743 , B01L3/5025 , B01L3/502715 , B01L3/502761 , B01L3/5085 , B01L3/545 , B01L2200/0668 , B01L2300/021 , B01L2300/0819 , B01L2300/0829 , B01L2400/0454 , B01L2400/0457 , B82Y30/00 , C40B40/06 , C40B60/14 , G01N21/25 , G01N21/253 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/6489 , G01N33/54373 , G01N2015/0092 , G01N2015/1472 , G01N2015/1497 , G01N2021/6441 , G01N2021/6482 , G01N2035/00158 , G01N2035/00742 , G01N2035/00752 , G01N2201/04 , G01N2201/06113 , G01N2201/12
摘要： Devices, systems, methods, and compositions of matter can track and/or identify a library of elements, particularly for use with fluids, particulates, cells, and the like. Signals from one or more semiconductor nanocrystals may be combined to define spectral codes. Separation of signal wavelengths within dedicated wavelength ranges or windows facilitates differentiation of spectral codes, while calibration signals within the spectral codes can avoid ambiguity. Modeling based on prior testing can help derive libraries of acceptable codes.

7. 发明授权

US07079241B2 Spatial positioning of spectrally labeled beads 有权
公开(公告)号：US07079241B2
公开(公告)日：2006-07-18
申请号：US09827256
申请日：2001-04-05
申请人： Stephen A. Empedocles , Andrew R. Watson , Jian Jin
发明人： Stephen A. Empedocles , Andrew R. Watson , Jian Jin
IPC分类号： G01J3/28 , G01J3/00 , G01J3/30 , G01J3/42 , G01N21/29
CPC分类号： G01N21/272 , B01J2219/00317 , B01J2219/00432 , B01J2219/005 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00657 , B01J2219/00659 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00743 , B01L3/5025 , B01L3/502715 , B01L3/502761 , B01L3/5085 , B01L3/545 , B01L2200/0668 , B01L2300/021 , B01L2300/0819 , B01L2300/0829 , B01L2400/0454 , B01L2400/0457 , B82Y30/00 , C40B40/06 , C40B60/14 , G01N21/25 , G01N21/253 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/6489 , G01N33/54373 , G01N2015/0092 , G01N2015/1472 , G01N2015/1497 , G01N2021/6441 , G01N2021/6482 , G01N2035/00158 , G01N2035/00742 , G01N2035/00752 , G01N2201/04 , G01N2201/06113 , G01N2201/12
摘要： Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.

8. 发明授权

US06759235B2 Two-dimensional spectral imaging system 有权
公开(公告)号：US06759235B2
公开(公告)日：2004-07-06
申请号：US09827076
申请日：2001-04-05
申请人： Stephen A. Empedocles , Andrew R. Watson
发明人： Stephen A. Empedocles , Andrew R. Watson
IPC分类号： C12M134
CPC分类号： G01N21/272 , B01J2219/00317 , B01J2219/00432 , B01J2219/005 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00657 , B01J2219/00659 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00743 , B01L3/5025 , B01L3/502715 , B01L3/502761 , B01L3/5085 , B01L3/545 , B01L2200/0668 , B01L2300/021 , B01L2300/0819 , B01L2300/0829 , B01L2400/0454 , B01L2400/0457 , B82Y30/00 , C40B40/06 , C40B60/14 , G01N21/25 , G01N21/253 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/6489 , G01N33/54373 , G01N2015/0092 , G01N2015/1472 , G01N2015/1497 , G01N2021/6441 , G01N2021/6482 , G01N2035/00158 , G01N2035/00742 , G01N2035/00752 , G01N2201/04 , G01N2201/06113 , G01N2201/12
摘要： Improved devices, systems, and methods for sensing and/or identifying signals from within a signal detection region are well-suited for identification of spectral codes. Large numbers of independently identifiable spectral codes can be generated by quite small bodies, and a plurality of such bodies or probes may be present within a detection region. Simultaneously imaging of identifiable spectra from throughout the detection region allows the probes to be identified. As the identifiable spectra can be treated as being generated from a point source within a much larger detection field, a prism, diffractive grading, holographic transmissive grading, or the like can spectrally disperse the images of the labels across a sensor surface. A CCD can identify the relative wavelengths of signals making up the spectra. Absolute signal wavelengths may be identified by determining positions of the labels, by an internal wavelength reference within the spectra, or the like.

9. 发明授权

US06734420B2 Differentiable spectral bar code methods and systems 有权
标题翻译：可分辨光谱条形码方法和系统
公开(公告)号：US06734420B2
公开(公告)日：2004-05-11
申请号：US09827013
申请日：2001-04-05
申请人： Stephen A. Empedocles , Joseph A. Treadway , Andrew R. Watson
发明人： Stephen A. Empedocles , Joseph A. Treadway , Andrew R. Watson
IPC分类号： G01N2129
CPC分类号： G01N21/272 , B01J2219/00317 , B01J2219/00432 , B01J2219/005 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00657 , B01J2219/00659 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00743 , B01L3/5025 , B01L3/502715 , B01L3/502761 , B01L3/5085 , B01L3/545 , B01L2200/0668 , B01L2300/021 , B01L2300/0819 , B01L2300/0829 , B01L2400/0454 , B01L2400/0457 , B82Y30/00 , C40B40/06 , C40B60/14 , G01N21/25 , G01N21/253 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/6489 , G01N33/54373 , G01N2015/0092 , G01N2015/1472 , G01N2015/1497 , G01N2021/6441 , G01N2021/6482 , G01N2035/00158 , G01N2035/00742 , G01N2035/00752 , G01N2201/04 , G01N2201/06113 , G01N2201/12
摘要： Devices, systems, methods, and compositions of matter can track and/or identify a library of elements, particularly for use with fluids, particulates, cells, and the like. Signals from one or more semiconductor nanocrystals may be combined to define spectral codes. Separation of signal wavelengths within dedicated wavelength ranges or windows facilitates differentiation of spectral codes, while calibration signals within the spectral codes can avoid ambiguity. Modeling based on prior testing can help derive libraries of acceptable codes.
摘要翻译：物质的装置，系统，方法和组合物可以跟踪和/或识别元件库，特别是用于流体，微粒，细胞等。来自一个或多个半导体纳米晶体的信号可以被组合以限定光谱代码。信号波长在专用波长范围或窗口内的分离有助于光谱代码的分化，而光谱码内的校准信号可以避免歧义。基于先前测试的建模可以帮助导出可接受代码的库。

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