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1. 发明专利

DE602005022757D1 VERWENDUNG VON ENDOSIALINBINDENDEN PROTEINEN ZUM ISOLIEREN ENDOSIALINPOSITIVER ZELLEN 未知
公开(公告)号：DE602005022757D1
公开(公告)日：2010-09-16
申请号：DE602005022757
申请日：2005-12-02
申请人： MORPHOTEK INC
发明人： GRASSO LUIGI , DESHMUKH GAURAV , NICOLAIDES NICHOLAS , SASS PHILIP
IPC分类号： C07K16/28 , C12N5/10 , C12N15/13 , G01N33/50

2. 发明专利

AT444359T 未知
公开(公告)号：AT444359T
公开(公告)日：2009-10-15
申请号：AT03786774
申请日：2003-11-14
申请人： MORPHOTEK INC
发明人： GRASSO LUIGI , LIANG SHAOHONG , NICOLAIDES NICHOLAS , SASS PHILIP
IPC分类号： C12N15/10 , C07K16/00 , C07K16/12 , C07K16/24 , C07K16/28 , C07K16/30 , C12N5/08 , C12N5/10 , C12N5/16 , C12N15/01 , C12N15/06 , C12N15/13

3. 发明申请

WO2006060719A3 USE OF ENDOSIALIN BINDING PROTEINS TO ISOLATE ENDOSIALIN POSITIVE CELLS 审中-公开
标题翻译：使用内啡肽结合蛋白分离内皮素阳性细胞
公开(公告)号：WO2006060719A3
公开(公告)日：2006-11-23
申请号：PCT/US2005043749
申请日：2005-12-02
申请人： MORPHOTEK INC , GRASSO LUIGI , DESHMUKH GAURAV , NICOLAIDES NICHOLAS , SASS PHILIP
发明人： GRASSO LUIGI , DESHMUKH GAURAV , NICOLAIDES NICHOLAS , SASS PHILIP
IPC分类号： C07K16/28 , C12N5/10 , C12N15/13 , G01N33/50
CPC分类号： G01N33/574 , C07K16/2851 , G01N33/56966 , G01N33/57492 , G01N2333/515 , G01N2333/70596 , G01N2500/00 , G01N2800/10 , G01N2800/16 , G01N2800/24
摘要： Monoclonal antibodies and other proteins that specifically bind to cells positive for endosialin are provided. The antibodies and proteins are useful in the isolation of endosialin-positive cells, particularly cells associated with neovascularization associated with cancer and neovascular disease. The invention is also related to cells that are isolated using monoclonal antibodies; antibody derivatives, such as chimeric and humanized monoclonal antibodies; antibody fragments; mammalian cells expressing the monoclonal antibodies, derivatives and fragment; and methods of detecting and isolation endosialin-positive cells using the antibodies, derivatives and fragments.
摘要翻译：提供单克隆抗体和特异性结合内皮素的阳性细胞的其他蛋白质。抗体和蛋白质可用于分离内皮唾液酸阳性细胞，特别是与与癌症和新血管病相关的新生血管形成相关的细胞。本发明还涉及使用单克隆抗体分离的细胞; 抗体衍生物，例如嵌合和人源化单克隆抗体; 抗体片段; 哺乳动物细胞表达单克隆抗体，衍生物和片段; 以及使用抗体，衍生物和片段检测和分离内皮唾液酸 - 阳性细胞的方法。

4. 发明专利

AT501635T 未知
公开(公告)号：AT501635T
公开(公告)日：2011-04-15
申请号：AT01901992
申请日：2001-01-15
申请人： MORPHOTEK INC
发明人： NICHOLAIDES NICHOLAS , GRASSO LUIGI , SASS PHILIP
IPC分类号： A01H1/06 , A01H5/00 , A01H3/04 , A01K67/027 , A61K31/7076 , A61K31/7088 , C12N1/00 , C12N5/00 , C12N15/01 , C12N15/09 , C12N15/10 , C12N15/82 , C12Q1/02 , C12Q1/68 , G01N33/50

5. 发明专利

AT404665T 未知
公开(公告)号：AT404665T
公开(公告)日：2008-08-15
申请号：AT01907187
申请日：2001-02-12
申请人： UNIV JOHNS HOPKINS , MORPHOTEK INC
发明人： NICOLAIDES NICHOLAS , SASS PHILIP , GRASSO LUIGI , VOGELSTEIN BERT , KINZLER KENNETH
IPC分类号： C12N15/10 , C12N1/20 , C12N15/01
摘要： Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficiency and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch repair activity to the bacteria.

6. 发明专利

AT548461T 未知
公开(公告)号：AT548461T
公开(公告)日：2012-03-15
申请号：AT03703842
申请日：2003-01-17
申请人： MORPHOTEK INC
发明人： GRASSO LUIGI , KLINE J , NICOLAIDES NICHOLAS , SASS PHILIP
IPC分类号： C12N15/87 , A61K48/00 , C07H21/04 , C12N1/20 , C12N15/00 , C12N15/10 , C12N15/63 , C12N15/90 , C12Q1/68
摘要： Inhibitors of mismatch repair can be used to generate hypermutable cells and organisms. By inhibiting this process in cells, new cell lines and varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of homologous recombination. These methods are useful for generating targeted loci that can alter the expression profiles of target genes as well as tag exons of a gene with a reporter marker to facilitate the monitoring of a given gene product when the host is grown under different conditions or exposed to biological and chemical entities.

7. 发明专利

CA2590583A1 USE OF ENDOSIALIN BINDING PROTEINS TO ISOLATE ENDOSIALIN POSITIVE CELLS 未知
公开(公告)号：CA2590583A1
公开(公告)日：2006-06-08
申请号：CA2590583
申请日：2005-12-02
申请人： MORPHOTEK INC
发明人： SASS PHILIP , DESHMUKH GAURAV , GRASSO LUIGI , NICOLAIDES NICHOLAS
IPC分类号： C07K16/28 , C12N5/10 , C12N15/13 , G01N33/50
摘要： Monoclonal antibodies and other proteins that specifically bind to cells positive for endosialin are provided. The antibodies and proteins are useful in the isolation of endosialin-positive cells, particularly cells associated with neovascularization associated with cancer and neovascular disease. The invention is also related to cells that are isolated using monoclonal antibodies; antibody derivatives, such as chimeric and humanized monoclonal antibodies; antibody fragments; mammalian cells expressing the monoclonal antibodies, derivatives and fragments; and methods of detecting and isolating endosialin-positive cells using the antibodies, derivatives and fragments.

8. 发明专利

AT489474T 未知
公开(公告)号：AT489474T
公开(公告)日：2010-12-15
申请号：AT03765839
申请日：2003-07-21
申请人： MORPHOTEK INC
发明人： GRASSO LUIGI , KLINE BRADFORD J , NICOLAIDES NICHOLAS , SASS PHILIP
IPC分类号： C12P21/00 , G01N33/48 , C07K16/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/63 , C12N15/87 , C12P21/04 , C12Q1/02 , C12Q1/68 , C12Q1/70 , G01N33/53 , G01N33/68
摘要： The use of mismatch repair (MMR) defective antibody producer cells offers a method to generate subclone variants with elevated protein production such as antibodies. Using MMR defective cells and animals, new cell lines and animal varieties with novel and useful properties such as enhanced protein production can be generated more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within host cells to alter endogenous genes that can yield increased titer levels of protein production. By employing this method, two genes were discovered whose suppressed expression is associated with enhanced antibody production. Suppressed expression of these genes by a variety of methods leads to increased antibody production for manufacturing as well as strategies for modulating antibody production in immunological disorders. Moreover, the suppression of these two genes in host cells can be useful for generating universal high titer protein production lines.

9. 发明专利

AT476449T 未知
公开(公告)号：AT476449T
公开(公告)日：2010-08-15
申请号：AT05847825
申请日：2005-12-02
申请人： MORPHOTEK INC
发明人： GRASSO LUIGI , DESHMUKH GAURAV , NICOLAIDES NICHOLAS , SASS PHILIP
IPC分类号： C07K16/28 , C12N5/10 , C12N15/13 , G01N33/50
摘要： Monoclonal antibodies and other proteins that specifically bind to cells positive for endosialin are provided. The antibodies and proteins are useful in the isolation of endosialin-positive cells, particularly cells associated with neovascularization associated with cancer and neovascular disease. The invention is also related to cells that are isolated using monoclonal antibodies; antibody derivatives, such as chimeric and humanized monoclonal antibodies; antibody fragments; mammalian cells expressing the monoclonal antibodies, derivatives and fragments; and methods of detecting and isolating endosialin-positive cells using the antibodies, derivatives and fragments.

10. 发明专利

AT428804T 未知
公开(公告)号：AT428804T
公开(公告)日：2009-05-15
申请号：AT03733833
申请日：2003-02-21
申请人： MORPHOTEK INC
发明人： GRASSO LUIGI , NICOLAIDES NICHOLAS , SASS PHILIP
IPC分类号： C12Q1/68 , C12N15/10 , C12N15/85
摘要： 1. The present invention provides a method for producing an isolated, genetically stable somatic cell line with a new phenotype, the method comprising the steps of: a) culturing a recombinant host somatic cell comprising a vector, said vector comprising a constitutively active promoter operatively linked to a sequence encoding a dominant negative allele of a mismatch repair gene, an internal ribosome entry site, and a negative selection marker, under conditions for the expression of the dominant negative allele, rendering the recombinant host somatic cell hypermutable and thereby producing a library of cells; b) selecting for clones from the cell library exhibiting new phenotypes; c) from the clones selected in step (b), negatively selecting for clones no longer expressing the dominant negative allele of a mismatch repair gene, rendering the resulting subclones genetically stable; and d) expanding a genetically stable subclone of step (c) to generate said genetically stable cell line.

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