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1. 发明申请

WO2007047016A3 METHODS FOR PRODUCING HYBRID SEED 审中-公开
标题翻译：混合种子的生产方法
公开(公告)号：WO2007047016A3
公开(公告)日：2008-02-14
申请号：PCT/US2006036847
申请日：2006-09-20
申请人： MONSANTO TECHNOLOGY LLC , ALLEN EDWARDS , GILBERTSON LARRY A , HOUMARD NANCY M , HUANG SHIHSHIEH , IVASHUTA SERGEY I , ROBERTS JAMES K
发明人： ALLEN EDWARDS , GILBERTSON LARRY A , HOUMARD NANCY M , HUANG SHIHSHIEH , IVASHUTA SERGEY I , ROBERTS JAMES K
IPC分类号： C12N15/63 , A01H1/00 , A01H5/00 , A01H5/10 , C12N15/82 , C12N15/87 , C12N15/90
CPC分类号： C12N15/8218 , A01H1/02 , A01H5/00 , A01H5/10 , C12N15/8287 , C12N15/8289 , C12N15/829
摘要： This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.
摘要翻译：本发明提供了生产非天然杂种种子的方法。还公开了可用于在作物植物上赋予诱导型不育性的特异性miRNA和miRNA识别位点，以及包含这种外源miRNA识别位点的重组DNA构建体。

2. 发明申请

WO2006073727A3 RECOMBINANT DNA CONSTRUCTS AND METHODS FOR CONTROLLING GENE EXPRESSION 审中-公开
标题翻译：重组DNA构建体和控制基因表达的方法
公开(公告)号：WO2006073727A3
公开(公告)日：2007-06-28
申请号：PCT/US2005045517
申请日：2005-12-15
申请人： MONSANTO TECHNOLOGY LLC , HUANG SHIHSHIEH , MALVAR THOMAS M , LEUTHY MICHAEL H , MILLER PHILIP W , GILBERTSON LARRY A , ALLEN EDWARDS , HEISEL SARA E , KOVALIC DAVID E , ROBERTS JAMES K , HOUMARD NANCY M , FRIZZI ALESSANDRA , ZHANG YAUNJI
发明人： HUANG SHIHSHIEH , MALVAR THOMAS M , LEUTHY MICHAEL H , MILLER PHILIP W , GILBERTSON LARRY A , ALLEN EDWARDS , HEISEL SARA E , KOVALIC DAVID E , ROBERTS JAMES K , HOUMARD NANCY M , FRIZZI ALESSANDRA , ZHANG YAUNJI
IPC分类号： C12N5/10 , A01H1/00 , A01H5/00 , A01H5/10 , C12N15/82 , C12N15/90
CPC分类号： C12N15/8216 , C12N15/8217 , C12N15/8218 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译：本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用方法。本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。

3. 发明申请

WO2008133643A3 PLANT MICRORNAS AND METHODS OF USE THEREOF 审中-公开
标题翻译：植物微生物及其使用方法
公开(公告)号：WO2008133643A3
公开(公告)日：2009-05-14
申请号：PCT/US2007021987
申请日：2007-10-12
申请人： MONSANTO TECHNOLOGY LLC , ALLEN EDWARDS , GOLDMAN BARRY S , GUO LIANG , HEISEL SARA E , HUANG SHIHSHIEH , IVASHUTA SERGEY I , KRIEGER ELYSIA K , ROBERTS JAMES K , ZHANG YUANJI I
发明人： ALLEN EDWARDS , GOLDMAN BARRY S , GUO LIANG , HEISEL SARA E , HUANG SHIHSHIEH , IVASHUTA SERGEY I , KRIEGER ELYSIA K , ROBERTS JAMES K , ZHANG YUANJI I
IPC分类号： C12Q1/68 , A01H1/00
CPC分类号： C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8238 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N2310/141 , C12N2320/50 , Y02A40/146 , Y02A40/162
摘要： This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
摘要翻译：本发明公开了新的微小RNA及其前体，以及重组DNA构建体，包括这样的新型miRNA，miRNA前体，miRNA启动子和对应于miRNA的miRNA识别位点。包括显示营养反应表达的新型miRNA和miRNA前体。还公开了miRNA诱饵序列。还提供了非天然转基因植物细胞，植物和种子，在其基因组中含有本发明的重组DNA构建体和使用本发明的重组DNA构建体控制基因表达的方法。

4. 发明申请

WO2008133643A8 PLANT MICRORNAS AND METHODS OF USE THEREOF 审中-公开
标题翻译：植物微生物及其使用方法
公开(公告)号：WO2008133643A8
公开(公告)日：2011-10-06
申请号：PCT/US2007021987
申请日：2007-10-12
申请人： MONSANTO TECHNOLOGY LLC , ALLEN EDWARDS , GOLDMAN BARRY S , GUO LIANG , HEISEL SARA E , HUANG SHIHSHIEH , IVASHUTA SERGEY I , KRIEGER ELYSIA K , ROBERTS JAMES K , ZHANG YUANJI I
发明人： ALLEN EDWARDS , GOLDMAN BARRY S , GUO LIANG , HEISEL SARA E , HUANG SHIHSHIEH , IVASHUTA SERGEY I , KRIEGER ELYSIA K , ROBERTS JAMES K , ZHANG YUANJI I
IPC分类号： C12Q1/68 , A01H1/00
CPC分类号： C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8238 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N2310/141 , C12N2320/50 , Y02A40/146 , Y02A40/162
摘要： This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
摘要翻译：本发明公开了新的微小RNA及其前体，以及重组DNA构建体，包括这样的新型miRNA，miRNA前体，miRNA启动子和对应于miRNA的miRNA识别位点。包括显示营养反应表达的新型miRNA和miRNA前体。还公开了miRNA诱饵序列。还提供了非天然转基因植物细胞，植物和种子，在其基因组中含有本发明的重组DNA构建体和使用本发明的重组DNA构建体控制基因表达的方法。

5. 发明申请

WO2007011479A3 DOUBLE-STRANDED RNA STABILIZED IN PLANTA 审中-公开
标题翻译：双稳态RNA稳定在PLANTA
公开(公告)号：WO2007011479A3
公开(公告)日：2007-12-13
申请号：PCT/US2006023039
申请日：2006-06-13
申请人： MONSANTO TECHNOLOGY LLC , ALLEN EDWARDS , GILBERTSON LARRY A , KRIEGER ELYSIA K , ROBERTS JAMES K , STAUB JEFFREY M
发明人： ALLEN EDWARDS , GILBERTSON LARRY A , KRIEGER ELYSIA K , ROBERTS JAMES K , STAUB JEFFREY M
IPC分类号： C12N15/09 , A01H5/00 , A01H5/10 , C12N15/63 , C12N15/82 , C12N15/84
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： This invention discloses recombinant DNA constructs that transcribe to RNA having improved resistance to a plant RNase HI enzyme, and methods for their use in making transgenic plant cells, plants, seeds, and products derived therefrom. Also provided are compositions and methods for imparting to a plant resistance to a pest or pathogen of the plant.
摘要翻译：本发明公开了转录到对植物RNase HI酶具有改善的抗性的RNA的重组DNA构建体及其用于制备转基因植物细胞，植物，种子和由其衍生的产物的方法。还提供了赋予植物对植物的害虫或病原体的抗性的组合物和方法。

6. 发明申请

WO2008027592A3 PHASED SMALL RNAS 审中-公开
标题翻译： PHASED小RNAS
公开(公告)号：WO2008027592A3
公开(公告)日：2008-09-04
申请号：PCT/US2007019283
申请日：2007-08-31
申请人： MONSANTO TECHNOLOGY LLC , ALLEN EDWARDS , GUO LIANG , HEISEL SARA E , IVASHUTA SERGEY I , ZHANG YUANJI I
发明人： ALLEN EDWARDS , GUO LIANG , HEISEL SARA E , IVASHUTA SERGEY I , ZHANG YUANJI I
IPC分类号： C12N15/11 , A01H5/00 , C12N15/82
CPC分类号： C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8254 , C12N15/8266 , C12N15/827
摘要： This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.
摘要翻译：本发明公开了编码可用于调节一种或多种目的基因表达的定相小RNA的重组DNA构建体。本发明还公开了含有本发明的重组DNA构建体的转基因植物细胞，植物和种子。

7. 发明申请

WO2008027592A9 PHASED SMALL RNAS 审中-公开
标题翻译：相互作用的小RNA
公开(公告)号：WO2008027592A9
公开(公告)日：2008-04-24
申请号：PCT/US2007019283
申请日：2007-08-31
申请人： MONSANTO TECHNOLOGY LLC , ALLEN EDWARDS , GUO LIANG , HEISEL SARA E , IVASHUTA SERGEY I , ZHANG YUANJI I
发明人： ALLEN EDWARDS , GUO LIANG , HEISEL SARA E , IVASHUTA SERGEY I , ZHANG YUANJI I
IPC分类号： C12N15/11
CPC分类号： C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8254 , C12N15/8266 , C12N15/827
摘要： This invention discloses recombinant DNA constructs encoding phased small RNAs useful i
摘要翻译：本发明公开了编码分阶段的小RNA的重组DNA构建体，

8. 发明申请

WO2009003078A2 TEMPORAL REGULATION OF GENE EXPRESSION BY MICRORNAS 审中-公开
标题翻译： MICRORNAS进行基因表达的时间调控
公开(公告)号：WO2009003078A2
公开(公告)日：2008-12-31
申请号：PCT/US2008068276
申请日：2008-06-26
申请人： MONSANTO TECHNOLOGY LLC , ALLEN EDWARDS , HEISEL SARA E , IVASHUTA SERGEY I , KRIEGER ELYSIA K , LUTKE JENNIFER LYNN , MEISTER ROBERT JOSEPH , ZHANG YUANJI I
发明人： ALLEN EDWARDS , HEISEL SARA E , IVASHUTA SERGEY I , KRIEGER ELYSIA K , LUTKE JENNIFER LYNN , MEISTER ROBERT JOSEPH , ZHANG YUANJI I
IPC分类号： C12N15/82
CPC分类号： C12N15/8218 , C12N15/8216 , C12N15/8217 , C12N15/8225 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
摘要： This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
摘要翻译：本发明提供用于在植物或植物害虫或病原体中时间特异性控制基因表达的分子构建体和方法。更具体地，本发明提供具有新颖的昼夜节律表达模式的植物miRNA基因，其可用于设计用于至少一种基因的时间特异性表达的重组DNA构建体。还提供了在其基因组中含有本发明的重组DNA构建体的非天然转基因植物细胞，植物和种子。

9. 发明申请

WO2010039750A2 TRANSGENIC PLANTS WITH ENHANCED AGRONOMIC TRAITS 审中-公开
标题翻译：具有增强农业条件的转基因植物
公开(公告)号：WO2010039750A2
公开(公告)日：2010-04-08
申请号：PCT/US2009058908
申请日：2009-09-30
申请人： MONSANTO TECHNOLOGY LLC , AASEN ERIC , ABAD MARK S , ALLEN EDWARDS , AMBIKA THANDONI RAO , ANIL VEENA S , AUGUSTINE ALICE , BACK STEPHANIE , BADAMI PRANESH , BASRA AMARJIT , BRUSTAD KRAIG , DENG MOLIAN , DEZWAAN TODD , DIETRICH CHARLES R , DUFF STEPHEN M , GABBERT KAREN K , HE STEVE , HENDRIX BILL , JIA HONGWU , KAMMARADI RAMANATH BHAT VIDYA , KARUNANANDAA BALASULOJINI , KOSOLA KEVIN R , KRISHNAMURTHY K , KUMAR GANESH , KURIAKOSE SARITHA , LAKKANNA SHANTALA , LEIBMAN MARK , LUTFIYYA LINDA L , MADAPPA SAVITHA , MANJUNATH SIVALINGANNA , MEISTER ROBERT JOSEPH , PRASAD BELLUR NARASIMHA , PREUSS SASHA , QI QUNQANG , RAMACHANDRA DHANALAKSHMI , RAYCHAUDHURI ANIRUDDHA , SANGEETHA S , SARACCO SCOTT , SHARMA CHITRESH , SHARMA VIJAY , SOMAIAH RAJANI MONNANDA , SUDARSHANA PADMINI , THOMPSON-MIZE REBECCA , VENKATACHALAYYA SRIKANTH BABU , VENKATESH TYAMAGONDLU V , WANG HUAI , WU JINGRUI , WU XIAOYUN , YANG SEAN , ZENG QIN , ZHAO JIANMIN
发明人： AASEN ERIC , ABAD MARK S , ALLEN EDWARDS , AMBIKA THANDONI RAO , ANIL VEENA S , AUGUSTINE ALICE , BACK STEPHANIE , BADAMI PRANESH , BASRA AMARJIT , BRUSTAD KRAIG , DENG MOLIAN , DEZWAAN TODD , DIETRICH CHARLES R , DUFF STEPHEN M , GABBERT KAREN K , HE STEVE , HENDRIX BILL , JIA HONGWU , KAMMARADI RAMANATH BHAT VIDYA , KARUNANANDAA BALASULOJINI , KOSOLA KEVIN R , KRISHNAMURTHY K , KUMAR GANESH , KURIAKOSE SARITHA , LAKKANNA SHANTALA , LEIBMAN MARK , LUTFIYYA LINDA L , MADAPPA SAVITHA , MANJUNATH SIVALINGANNA , MEISTER ROBERT JOSEPH , PRASAD BELLUR NARASIMHA , PREUSS SASHA , QI QUNQANG , RAMACHANDRA DHANALAKSHMI , RAYCHAUDHURI ANIRUDDHA , SANGEETHA S , SARACCO SCOTT , SHARMA CHITRESH , SHARMA VIJAY , SOMAIAH RAJANI MONNANDA , SUDARSHANA PADMINI , THOMPSON-MIZE REBECCA , VENKATACHALAYYA SRIKANTH BABU , VENKATESH TYAMAGONDLU V , WANG HUAI , WU JINGRUI , WU XIAOYUN , YANG SEAN , ZENG QIN , ZHAO JIANMIN
IPC分类号： C12N15/82 , A23L11/00
CPC分类号： C12N15/8251 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8275 , Y02A40/146
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced trait.
摘要翻译：本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率，种子蛋白和增强种子油。还公开了用于制备具有增强特征的转基因种子和植物的方法。

10. 发明专利

ES2655700T3 ARN pequeños en fase 未知
公开(公告)号：ES2655700T3
公开(公告)日：2018-02-21
申请号：ES07837683
申请日：2007-08-31
申请人： MONSANTO TECHNOLOGY LLC
发明人： ALLEN EDWARDS , GUO LIANG , HEISEL SARA E , IVASHUTA SERGEY I , ZHANG YUANJI I
IPC分类号： C12N15/11 , A01H5/00 , C12N15/82
摘要： Una construcción de ADN recombinante para la supresión génica que comprende un promotor funcional en células vegetales, operativamente unido a ADN recombinante que codifica un transcrito precursor de ARN pequeño en fase sintético y que comprende una secuencia de nucleótidos de una secuencia molde de ARN pequeño en fase, en la que dicho promotor es heterólogo de dicho ADN recombinante y en la que dicha secuencia de molde de ARN pequeño en fase comprende una secuencia de nucleótidos seleccionada de la SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO. 17, SEQ ID NO. 33, SEQ ID NO. 69, y la secuencia de ADN que codifica la SEQ ID NO. 34, en la que dicho transcrito precursor de ARN pequeño en fase sintético preserva la estructura secundaria del transcrito de dicha secuencia molde de ARN pequeño en fase, en la que dicha estructura secundaria comprende ARN hibridado que forma una estructura plegada que incluye dos brazos antiparalelos de cadena sencilla, en la que un brazo es sustancial, pero no perfectamente, complementario con el otro brazo, en la que el emparejamiento de bases entre los dos brazos de la estructura replegada da como resultado un ARN de doble cadena sustancialmente pero no perfectamente que incluye apareamientos erróneos, en el que dicho ARN hibridado comprende al menos tres ARN bicatenarios pequeños en fase contiguos, en la que cada ARN bicatenario pequeño en fase comprende dos segmentos de ARN antiparalelo de aproximadamente 20 a aproximadamente 27 nucleótidos, cada uno con al menos un apareamiento erróneo entre dichos segmentos por lo que dichos apareamientos erróneos se distribuyen a lo largo de dicho ARN hibridado, y en la que en dicho transcrito de precursor de ARN pequeño en fase sintético, al menos uno de los segmentos de ARN se modifica para suprimir al menos un gen diana reemplazando los nucleótidos de un segmento de ARN de al menos uno de dichos ARN de doble cadena pequeños en fase en dicha secuencia de molde de ARN pequeño en fase por nucleótidos correspondientes a dicho al menos un gen diana, en la que dicho al menos un gen diana se selecciona de entre múltiples segmentos no consecutivos de un gen diana, múltiples alelos de un gen diana o múltiples genes diana de una o más especies, en la que al menos una de dichas dos cadenas antiparalelas de dicho ARN bicatenario pequeño en fase es aproximadamente un 95 % complementaria de dicho al menos un gen diana, y en la que dicho promotor es heterólogo de dicho ADN recombinante.

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