专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

JPH0759575A EXPRESSION-CONTROLLING DNA 失效
公开(公告)号：JPH0759575A
公开(公告)日：1995-03-07
申请号：JP20646193
申请日：1993-08-20
申请人： MITSUBISHI CORP , MITSUBISHI CHEM CORP
发明人： NAKAJIMA MIDORI , SHIMAMOTO ISAO
IPC分类号： A01H5/00 , C12N5/10 , C12N15/09
摘要： PURPOSE:To obtain the subject DNA contained in a 5' upper stream region from the transcription starting point of a waxy gene strongly expressed in the seed of a gramineous plant and capable of being bound to an extraneous gene to improve the ingredients of the seed or produce a substance in the seed. CONSTITUTION:A genomic DNA prepared from the leaf of rice by a conventional method is cleaved with a restriction enzyme, and subsequently ligated with lambda-phage to make a library. The library is subjected to a plaque hybridization using the waxy cDNA of the rice and a positive clone is screened. The positive clone is treated with a restriction enzyme to form reporter vectors successively deleted from the 5'-upper region. The presence or absence of the expressions of the reporter genes are measured to specify an expression- controlling region to obtain the objective expression-controlling DNA having a basic sequence of the formula, etc., contained in a 5'-upper flow region upper by -637 to 275 bases from the transcription-starting point of the rice waxy gene, and capable of being bound to an extraneous gene and introduced in a gramineous plant to improve the ingredients of the seed and produce a substance with the seed.

2. 发明专利

JPH07274752A GRAMINEOUS PLANT HAVING DISEASE RESISTANCE AND METHOD FOR CREATING THE SAME PLANT 失效
公开(公告)号：JPH07274752A
公开(公告)日：1995-10-24
申请号：JP9803894
申请日：1994-04-11
申请人： MITSUBISHI CORP , MITSUBISHI CHEM CORP
发明人： KIMURA YUSUKE , FUJIMOTO HIDEYA , YOSHIKAWA MASAAKI , TANAKA AKIRA , YAMAMOTO MIKIHIRO , SHIMAMOTO ISAO
IPC分类号： A01H5/00 , C12N5/10 , C12N9/24 , C12N15/09
摘要： PURPOSE:To create a gramineous plant exhibiting a resistance to a disease damage such as rice sheath blight or rice bacterial leaf blight. CONSTITUTION:A promoter functioned in a gramineous plant, a vector having a DNA sequence coding for beta-1,3-endoglucanase and a terminator and a protoplast derived from the gramineous plant are suspended in a liquid medium and the vector is introduced into the protoplast and then, the protoplast is cultured in a medium containing a gramineous plant culturing cell to form a colony and a plant body is regenerated from the colony.

3. 发明专利

JPH03164186A ISOLATION OF PROMOTER 失效
公开(公告)号：JPH03164186A
公开(公告)日：1991-07-16
申请号：JP30238189
申请日：1989-11-21
申请人： MITSUBISHI CHEM IND , MITSUBISHI CORP
发明人： SHIMAMOTO ISAO , NAKAJIMA MIDORI
IPC分类号： C12N15/09 , C12N15/82
摘要： PURPOSE:To readily obtain a promoter efficiently manifesting exotic gene within plant cell from genome by introducing marker gene lacking of promoter into protoplast derived from plant and performing special treatment to said transformant cell. CONSTITUTION:At first, protoplast derived from plant and marker gene not containing promoter are suspended in a liquid medium and the marker gene is introduced into the protoplast with applying electric pulse. Next, genome DNA extracted from resultant marker gene-manifesting transformant cell is cut with restricting enzyme not cutting marker gene and said DNA fragment is subjected to self-cyclization, then said cyclic DNA is modified by heat to resolve to single chain. Next, respective 5-terminals of positive chains and negative chains on said heat-modified cyclic DNA are annealed together with primer DNA complementary to said DNA and heat-resistant DNA polymerase is affected to afford DNA having complementary chain. Furthermore, said DNA having complementary chain is amplified and the aimed DNA containing marker gene-manifesting promoter is obtained from said DNA.

4. 发明专利

JPS62232382A CULTIVATION OF RICE PROTOPLAST 失效
公开(公告)号：JPS62232382A
公开(公告)日：1987-10-12
申请号：JP7570086
申请日：1986-04-02
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND
发明人： SHIMAMOTO ISAO , HAYASHI YASUYUKI , KAERIYAMA JUNKO
IPC分类号： C12N5/10 , A01G1/00 , A01H4/00 , C12N5/00 , C12N5/04 , C12R1/91
摘要： PURPOSE:To produce a plant having novel properties comprising high colony- forming ratio and reproducibility and little difference between species, by preparing a protoplast from a callus originated from well-ripened grain of rice and culturing the protoplast in a medium containing cultured rice cell. CONSTITUTION:A callus is induced from a well-ripened grain of rice such as KOSHIHIKARI (a sperior kind of rice) by removing an awn from the grain, sterilizing the grain and culturing in an agar medium, etc. The collected callus is treated in a liquid containing cell wall-decomposition enzyme and filtered. The filtrate is added with KMC solution, etc., and centrifuged to obtain purified protoplast. The protoplast is solidified with an agarose-containing KM medium, etc., in the form of thin sheet and the solidified agarose gel is cultured in dark place under shaking in the above medium in the presence of cultured rice cell. The obtained colony is cultured in a proliferation medium and the grown callus is cultured in a redifferentiation medium to obtain a transplantable plant.

5. 发明专利

JPH06276872A ELIMINATION OF MARKER GENE 失效
公开(公告)号：JPH06276872A
公开(公告)日：1994-10-04
申请号：JP7380293
申请日：1993-03-31
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND
发明人： SHIMAMOTO ISAO , KIMURA YUSUKE
IPC分类号： A01H1/06
摘要： PURPOSE:To provide a maker gene eliminating method so designed that descendants after the next generation of transformant having an extraneous gene and marker gene respectively derived from foreign plasmid are raised and varieties having the extraneous gene alone are selected to selectively eliminate the marker gene needless for the plant. CONSTITUTION:A protoplast is prepared from a suspension made from callus derived from the fully ripen seeds of a plant (e.g. rice) and incorporated with plural foreign plasmids each having extraneous gene (e.g. stripe virus envelope gene) and marker gene (e.g. hygromycin phosphotransferase gene) respectively derived from foreign plasmid to effect transformation. The resultant transformant plant is cultured to regenerate the plant, and the descendants after the next generation of this regenerated plant are raised and the varieties having the extraneous gene alone to selectively eliminate the extraneous marker gene needless for the plant.

6. 发明专利

JPH01228478A GENE CODING INCLUSION PROTEIN OF CAULIFLOWER MOSAIC VIRUS AND PLANT TRANSFORMED WITH SAID GENE 失效
公开(公告)号：JPH01228478A
公开(公告)日：1989-09-12
申请号：JP5432088
申请日：1988-03-08
申请人： MITSUBISHI CHEM IND , MITSUBISHI CORP
发明人： TAKAHASHI HIDEKI , SHIMAMOTO ISAO , SUZUKI MASAHIKO , EBARA YOSHIO
IPC分类号： A01H1/00 , A01H5/00 , C12N15/00 , C12N15/09 , C12N15/82 , C12R1/91
摘要： PURPOSE:To provide a gene having a specific base sequence, coding an inclusion protein of cauliflower mosaic virus and useful for the production of transformed plant resistant to cauliflower mosaic virus. CONSTITUTION:The gene has a sequence of 1569 bases shown by the table (the description of the 232nd base and thereafter is omitted) and codes an inclusion protein of cauliflower mosaic virus (abbreviated as CaMV). The gene can be prepared by inoculating and proliferating CaMV-S to a leaf of turnip, extracting and purifying CaMV.DNA from the CaMV-infected leaf, cutting the obtained CaMV.DNA and subjecting to cloning, etc. A plant resistant to the virus can be prepared by transforming a plant with the obtained gene.

7. 发明专利

JPS63112936A PRODUCTION OF TRANSFORMED BRASSICA PLANT 失效
公开(公告)号：JPS63112936A
公开(公告)日：1988-05-18
申请号：JP25796786
申请日：1986-10-29
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND
发明人： TAKAHASHI HIDEKI , TANAKA AKIRA , SHIMAMOTO ISAO , SUZUKI MASAHIKO
IPC分类号： A01H1/00 , A01H5/00 , C12N15/00 , C12N15/09

8. 发明专利

JPH06141718A METHOD FOR CREATING LOW TEMPERATURE RESISTANT RICE PLANT BODY 失效
公开(公告)号：JPH06141718A
公开(公告)日：1994-05-24
申请号：JP27737692
申请日：1992-10-15
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND , TOHOKU ELECTRIC POWER CO
发明人： HAYASHI YASUYUKI , TANAKA TAKAYUKI , KURIHARA HIROYUKI , HORIBATA AKIRA , SHIMAMOTO ISAO , SUGAWARA YUKIHIRO , WATANABE CHIKANAGA
IPC分类号： A01H1/06
摘要： PURPOSE:To efficiently creat a low temperature resistant rice plant body capable providing low temperature resistance in the young plant body by subjecting calluses of rice to low temperature treatment, selecting a low temperature resistant callus and then redifferentiating rice young plant from this callus. CONSTITUTION:Rice calluses are treated with a cell wall-decomposing enzyme to prepare a protoplast and the protoplast is irradiated with ultraviolet rays from a distance of 30cm for 60 sec to induce variation and the variant is amhedded in an agar medium and cultured under dark conditions at 28 deg.C for 10-40 days to form a micro colony. Then, the colony is moved to a medium containing a fatty acid-unsaturating enzyme inhibitor such as 4- chloro-5-(dimethylamino)-2-phenyl-3 (2H)-pridazinone together with agar piece and cultured at 28 deg.C and 2000 1ux for 10 day and then subjected to low temperature treatment at 5 deg.C under dark conditions for 30 day and again cultured at 28 deg.C and 2000 lux and low temperature resistant viable callus is selected and young rice plant is redifferentiated from this callus to creat the objective low-temperature resistant rice plant body.

9. 发明专利

JPH0684A GENE OF INSECTICIDAL PROTEIN, GRAMINEOUS PLANT TRANSFOMED WITH THE SAME GENE AND ITS PRODUCTION 失效
公开(公告)号：JPH0684A
公开(公告)日：1994-01-11
申请号：JP30492692
申请日：1992-10-16
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND
发明人： FUJIMOTO HIDEYA , ITO KIMIKO , YAMAMOTO MIKIHIRO , SHIMAMOTO ISAO
IPC分类号： A01H1/00 , A01H5/00 , C12N5/10 , C12N15/09 , C12N15/32 , C12N15/83 , C12R1/91
摘要： PURPOSE:To obtain a new gene useful for variation to produce a gramineous plant of insect-resistant plant. CONSTITUTION:A gene encoding an insecticidal protein is varied by adaptability to codon utility ratio found in a gene of gramineous plant and, for example, has a base sequence of the formula. The gene is synthesized by changing only the kinds of codon in each amino acid without changing an amino acid sequence based on the amino acid sequence of natural Bt toxin protein.

10. 发明专利

JPH05153981A GRAMINEOUS PLANT CHANGED IN AMYLOSE CONTENT AND ITS PRODUCTION 失效
公开(公告)号：JPH05153981A
公开(公告)日：1993-06-22
申请号：JP31693591
申请日：1991-11-29
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND
发明人： NAKAJIMA MIDORI , TERADA RIE , ITO KIMIKO , SHIMAMOTO ISAO
IPC分类号： A01H5/00 , A01H5/10 , C12N5/10 , C12N15/09 , C12N15/54
摘要： PURPOSE:To obtain the subject plant improved in taste of rice by introducing a vector obtained by introducing sense NDA of a rice starch synthetase gene into the downstream of a specific promotor into a protoplast of rice to form a colony and regenerating a plant body from the colony. CONSTITUTION:A vector obtained by introducing sense NDA or anti-DNA of a rice starch synthetase gene into the downstream of a promotor expressed by albumen is mixed with a protoplast suspension derived from Gramineae plant such as glutinous rice and a hygromycin phosphotransferase gene is added to the mixture and electric pulse is applied thereto to introduce the vector into the plasmid and the plasmid is cultured in a culture medium containing rice culturing cell to form a colony and a plant body is regenerated from the colony to provide the objective Gramineous plant artificially improved in taste of rice by changing amylose content of rice.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式