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1. 发明专利

JPH06141718A METHOD FOR CREATING LOW TEMPERATURE RESISTANT RICE PLANT BODY 失效
公开(公告)号：JPH06141718A
公开(公告)日：1994-05-24
申请号：JP27737692
申请日：1992-10-15
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND , TOHOKU ELECTRIC POWER CO
发明人： HAYASHI YASUYUKI , TANAKA TAKAYUKI , KURIHARA HIROYUKI , HORIBATA AKIRA , SHIMAMOTO ISAO , SUGAWARA YUKIHIRO , WATANABE CHIKANAGA
IPC分类号： A01H1/06
摘要： PURPOSE:To efficiently creat a low temperature resistant rice plant body capable providing low temperature resistance in the young plant body by subjecting calluses of rice to low temperature treatment, selecting a low temperature resistant callus and then redifferentiating rice young plant from this callus. CONSTITUTION:Rice calluses are treated with a cell wall-decomposing enzyme to prepare a protoplast and the protoplast is irradiated with ultraviolet rays from a distance of 30cm for 60 sec to induce variation and the variant is amhedded in an agar medium and cultured under dark conditions at 28 deg.C for 10-40 days to form a micro colony. Then, the colony is moved to a medium containing a fatty acid-unsaturating enzyme inhibitor such as 4- chloro-5-(dimethylamino)-2-phenyl-3 (2H)-pridazinone together with agar piece and cultured at 28 deg.C and 2000 1ux for 10 day and then subjected to low temperature treatment at 5 deg.C under dark conditions for 30 day and again cultured at 28 deg.C and 2000 lux and low temperature resistant viable callus is selected and young rice plant is redifferentiated from this callus to creat the objective low-temperature resistant rice plant body.

2. 发明专利

JPH03164186A ISOLATION OF PROMOTER 失效
公开(公告)号：JPH03164186A
公开(公告)日：1991-07-16
申请号：JP30238189
申请日：1989-11-21
申请人： MITSUBISHI CHEM IND , MITSUBISHI CORP
发明人： SHIMAMOTO ISAO , NAKAJIMA MIDORI
IPC分类号： C12N15/09 , C12N15/82
摘要： PURPOSE:To readily obtain a promoter efficiently manifesting exotic gene within plant cell from genome by introducing marker gene lacking of promoter into protoplast derived from plant and performing special treatment to said transformant cell. CONSTITUTION:At first, protoplast derived from plant and marker gene not containing promoter are suspended in a liquid medium and the marker gene is introduced into the protoplast with applying electric pulse. Next, genome DNA extracted from resultant marker gene-manifesting transformant cell is cut with restricting enzyme not cutting marker gene and said DNA fragment is subjected to self-cyclization, then said cyclic DNA is modified by heat to resolve to single chain. Next, respective 5-terminals of positive chains and negative chains on said heat-modified cyclic DNA are annealed together with primer DNA complementary to said DNA and heat-resistant DNA polymerase is affected to afford DNA having complementary chain. Furthermore, said DNA having complementary chain is amplified and the aimed DNA containing marker gene-manifesting promoter is obtained from said DNA.

3. 发明专利

JPS62232382A CULTIVATION OF RICE PROTOPLAST 失效
公开(公告)号：JPS62232382A
公开(公告)日：1987-10-12
申请号：JP7570086
申请日：1986-04-02
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND
发明人： SHIMAMOTO ISAO , HAYASHI YASUYUKI , KAERIYAMA JUNKO
IPC分类号： C12N5/10 , A01G1/00 , A01H4/00 , C12N5/00 , C12N5/04 , C12R1/91
摘要： PURPOSE:To produce a plant having novel properties comprising high colony- forming ratio and reproducibility and little difference between species, by preparing a protoplast from a callus originated from well-ripened grain of rice and culturing the protoplast in a medium containing cultured rice cell. CONSTITUTION:A callus is induced from a well-ripened grain of rice such as KOSHIHIKARI (a sperior kind of rice) by removing an awn from the grain, sterilizing the grain and culturing in an agar medium, etc. The collected callus is treated in a liquid containing cell wall-decomposition enzyme and filtered. The filtrate is added with KMC solution, etc., and centrifuged to obtain purified protoplast. The protoplast is solidified with an agarose-containing KM medium, etc., in the form of thin sheet and the solidified agarose gel is cultured in dark place under shaking in the above medium in the presence of cultured rice cell. The obtained colony is cultured in a proliferation medium and the grown callus is cultured in a redifferentiation medium to obtain a transplantable plant.

4. 发明专利

JPH0684A GENE OF INSECTICIDAL PROTEIN, GRAMINEOUS PLANT TRANSFOMED WITH THE SAME GENE AND ITS PRODUCTION 失效
公开(公告)号：JPH0684A
公开(公告)日：1994-01-11
申请号：JP30492692
申请日：1992-10-16
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND
发明人： FUJIMOTO HIDEYA , ITO KIMIKO , YAMAMOTO MIKIHIRO , SHIMAMOTO ISAO
IPC分类号： A01H1/00 , A01H5/00 , C12N5/10 , C12N15/09 , C12N15/32 , C12N15/83 , C12R1/91
摘要： PURPOSE:To obtain a new gene useful for variation to produce a gramineous plant of insect-resistant plant. CONSTITUTION:A gene encoding an insecticidal protein is varied by adaptability to codon utility ratio found in a gene of gramineous plant and, for example, has a base sequence of the formula. The gene is synthesized by changing only the kinds of codon in each amino acid without changing an amino acid sequence based on the amino acid sequence of natural Bt toxin protein.

5. 发明专利

JPH05153981A GRAMINEOUS PLANT CHANGED IN AMYLOSE CONTENT AND ITS PRODUCTION 失效
公开(公告)号：JPH05153981A
公开(公告)日：1993-06-22
申请号：JP31693591
申请日：1991-11-29
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND
发明人： NAKAJIMA MIDORI , TERADA RIE , ITO KIMIKO , SHIMAMOTO ISAO
IPC分类号： A01H5/00 , A01H5/10 , C12N5/10 , C12N15/09 , C12N15/54
摘要： PURPOSE:To obtain the subject plant improved in taste of rice by introducing a vector obtained by introducing sense NDA of a rice starch synthetase gene into the downstream of a specific promotor into a protoplast of rice to form a colony and regenerating a plant body from the colony. CONSTITUTION:A vector obtained by introducing sense NDA or anti-DNA of a rice starch synthetase gene into the downstream of a promotor expressed by albumen is mixed with a protoplast suspension derived from Gramineae plant such as glutinous rice and a hygromycin phosphotransferase gene is added to the mixture and electric pulse is applied thereto to introduce the vector into the plasmid and the plasmid is cultured in a culture medium containing rice culturing cell to form a colony and a plant body is regenerated from the colony to provide the objective Gramineous plant artificially improved in taste of rice by changing amylose content of rice.

6. 发明专利

JPH01181791A PRODUCTION OF TRANSFORMED GRAMINEAE FAMILY PLANT 失效
公开(公告)号：JPH01181791A
公开(公告)日：1989-07-19
申请号：JP456588
申请日：1988-01-12
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND
发明人： SHIMAMOTO ISAO , TERADA RIE , SUZUKI MASAHIKO , AOYANAGI MIDORI
IPC分类号： A01H1/00 , C12N5/10 , C12N15/09 , C12N15/82
摘要： PURPOSE:To efficiently provide the title bred plant, by suspending a plasmid having a foreign gene, etc., and a protoplast originated from a gramineae family plant, applying electric pulses to the resultant suspension to transform the protoplast, culturing the transformed protoplast to form a colony and subsequently regenerating the plant body from the colony. CONSTITUTION:The objective transformed gramineae family plant is provided by suspending a plasmid having a promoter acting in a graminae gamily plant, a foreign gene (e.g., hygromycin phosphotransferase gene) and a terminator and a protoplast originated from a gramineae family plant (e.g., rice plant) in a liquid medium, applying electric pulses to the resultant suspension to introduce the plasmid into the protoplast, culturing the transformed protoplast in a medium containing the cultured cells of the gramineae family plant to form a colony and subsequently regenerating the plant body from the colony.

7. 发明专利

JPS6188874A Cultivation of protoplast 失效
标题翻译：原始植物的培养
公开(公告)号：JPS6188874A
公开(公告)日：1986-05-07
申请号：JP20913284
申请日：1984-10-05
申请人： Mitsubishi Chem Ind Ltd , Mitsubishi Corp
发明人： YAHIRO YOSHITERU , SHIMAMOTO ISAO , YAMASHITA YUMIKO
IPC分类号： C12N5/04 , A01G1/00 , A01H4/00 , C12N5/00 , C12R1/91
摘要： PURPOSE: To culture a protoplast separated from a plant of e.g. Brassicaceae family, in high efficiency, division frequency and reproducibility, by embedding the protoplast in agarose, culturing the agarose in a liquid medium free from NH
4
+ , and transferring to a liquid medium containing NH
4
+ .
CONSTITUTION: Protoplast separated from a plant of Brassicaceae family or Solanaceae family is embedded in agarose. The agarose is cultured first in a liquid medium free from NH
4
+ , and then in a liquid medium containing NH
4
+ (e.g. NH
4 NO
3 , etc.).
COPYRIGHT: (C)1986,JPO&Japio
摘要翻译：目的：培养从植物分离的原生质体。通过将原生质体包埋在琼脂糖中，在不含NH4 +的液体培养基中培养琼脂糖，并转移到含有NH4 +的液体培养基中，以高效率，分裂频率和再现性，以甘蓝科植物科，高效率，分裂频率和重现性。构成：将从青蒿科或茄科科植物分离的原生质体嵌入琼脂糖中。首先将琼脂糖在不含NH 4 +的液体培养基中，然后在含有NH 4 +（例如NH 4 NO 3等）的液体培养基中培养。

8. 发明专利

JPH06276872A ELIMINATION OF MARKER GENE 失效
公开(公告)号：JPH06276872A
公开(公告)日：1994-10-04
申请号：JP7380293
申请日：1993-03-31
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND
发明人： SHIMAMOTO ISAO , KIMURA YUSUKE
IPC分类号： A01H1/06
摘要： PURPOSE:To provide a maker gene eliminating method so designed that descendants after the next generation of transformant having an extraneous gene and marker gene respectively derived from foreign plasmid are raised and varieties having the extraneous gene alone are selected to selectively eliminate the marker gene needless for the plant. CONSTITUTION:A protoplast is prepared from a suspension made from callus derived from the fully ripen seeds of a plant (e.g. rice) and incorporated with plural foreign plasmids each having extraneous gene (e.g. stripe virus envelope gene) and marker gene (e.g. hygromycin phosphotransferase gene) respectively derived from foreign plasmid to effect transformation. The resultant transformant plant is cultured to regenerate the plant, and the descendants after the next generation of this regenerated plant are raised and the varieties having the extraneous gene alone to selectively eliminate the extraneous marker gene needless for the plant.

9. 发明专利

JPH01228478A GENE CODING INCLUSION PROTEIN OF CAULIFLOWER MOSAIC VIRUS AND PLANT TRANSFORMED WITH SAID GENE 失效
公开(公告)号：JPH01228478A
公开(公告)日：1989-09-12
申请号：JP5432088
申请日：1988-03-08
申请人： MITSUBISHI CHEM IND , MITSUBISHI CORP
发明人： TAKAHASHI HIDEKI , SHIMAMOTO ISAO , SUZUKI MASAHIKO , EBARA YOSHIO
IPC分类号： A01H1/00 , A01H5/00 , C12N15/00 , C12N15/09 , C12N15/82 , C12R1/91
摘要： PURPOSE:To provide a gene having a specific base sequence, coding an inclusion protein of cauliflower mosaic virus and useful for the production of transformed plant resistant to cauliflower mosaic virus. CONSTITUTION:The gene has a sequence of 1569 bases shown by the table (the description of the 232nd base and thereafter is omitted) and codes an inclusion protein of cauliflower mosaic virus (abbreviated as CaMV). The gene can be prepared by inoculating and proliferating CaMV-S to a leaf of turnip, extracting and purifying CaMV.DNA from the CaMV-infected leaf, cutting the obtained CaMV.DNA and subjecting to cloning, etc. A plant resistant to the virus can be prepared by transforming a plant with the obtained gene.

10. 发明专利

JPS63112936A PRODUCTION OF TRANSFORMED BRASSICA PLANT 失效
公开(公告)号：JPS63112936A
公开(公告)日：1988-05-18
申请号：JP25796786
申请日：1986-10-29
申请人： MITSUBISHI CORP , MITSUBISHI CHEM IND
发明人： TAKAHASHI HIDEKI , TANAKA AKIRA , SHIMAMOTO ISAO , SUZUKI MASAHIKO
IPC分类号： A01H1/00 , A01H5/00 , C12N15/00 , C12N15/09

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