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    • 9. 发明申请
    • METHODS FOR MUTATING GENES IN CELLS AND ANIMALS USING INSERTIONAL MUTAGENESIS
    • 在细胞和动物中使用插入性突变体诱导基因的方法
    • WO2003040324A2
    • 2003-05-15
    • PCT/US2002/035405
    • 2002-11-04
    • ATHERSYS, INC.HARRINGTON, John, JosephJACKSON, Paul, DavidJIANG, Li
    • HARRINGTON, John, JosephJACKSON, Paul, DavidJIANG, Li
    • C12N
    • C12N15/86A01K2217/075A01K2267/03C12N2740/10043C12N2800/60C12N2800/90C12N2840/203C12N2840/206C12N2840/44C12N2840/445
    • The present invention is in the fields of molecular biology, cell biology, and genetics. The invention is directed generally to mutating genes in cells in vitro and in multi-cellular organisms. The invention encompasses methods for mutating genes in cells using polynucleotides that act as insertional mutagens. Such methods are used to achieve mutation of a single gene to achieve a desired phenotype as well as mutation of multiple genes, required cumulatively to achieve a desired phenotype, in a cell or in a multi-cellular organism. The invention is also directed to methods of identifying one or more mutated genes, made by the methods of the invention, in cells and in multi-cellular organisms, by means of a tagging property provided by the insertional mutagen(s). The insertional mutagen thus allows identification of one or more genes that are mutated by insertion of an insertional mutagen. The invention is also directed to methods for correlating a phenotype with a gene by screening or selecting cells that have been mutated by an insertional mutagen incorporated into one or more genes in a cell and identifying the gene or genes causing the phenotype by means of a tagging property in one or more of the insertional mutagens. The invention is also directed to cells and multi-cellular organisms created by the methods of the invention and uses of the cells and multicellular organisms. The invention is also directed to libraries of cells created by the methods of the invention and uses of the libraries.
    • 本发明涉及分子生物学,细胞生物学和遗传学领域。 本发明一般涉及体外和多细胞生物体中细胞中的突变基因。 本发明包括使用充当插入诱变剂的多核苷酸突变细胞中的基因的方法。 这样的方法用于实现单个基因的突变,以实现期望的表型以及在细胞或多细胞生物体中累积需要累积所需表型的多个基因的突变。 本发明还涉及通过本发明的方法在细胞和多细胞生物体中通过插入诱变剂提供的标记特性鉴定一种或多种突变基因的方法。 因此,插入型诱变剂允许鉴定通过插入插入型突变体而突变的一种或多种基因。 本发明还涉及通过筛选或选择已经通过掺入细胞中的一个或多个基因的插入突变体突变的细胞并通过标记鉴定引起表型的基因或基因来将表型与基因相关联的方法 属性在一个或多个插入诱变剂。 本发明还涉及由本发明的方法和细胞和多细胞生物体的用途产生的细胞和多细胞生物体。 本发明还涉及通过本发明的方法和图书馆的使用而创建的细胞库。