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1. 发明申请

WO2003040324A3 METHODS FOR MUTATING GENES IN CELLS AND ANIMALS USING INSERTIONAL MUTAGENESIS 审中-公开
公开(公告)号：WO2003040324A3
公开(公告)日：2003-05-15
申请号：PCT/US2002/035405
申请日：2002-11-04
申请人： ATHERSYS, INC. , HARRINGTON, John, Joseph , JACKSON, Paul, David , JIANG, Li
发明人： HARRINGTON, John, Joseph , JACKSON, Paul, David , JIANG, Li
IPC分类号： C12N5/00
摘要： The present invention is in the fields of molecular biology, cell biology, and genetics. The invention is directed generally to mutating genes in cells in vitro and in multi-cellular organisms. The invention encompasses methods for mutating genes in cells using polynucleotides that act as insertional mutagens. Such methods are used to achieve mutation of a single gene to achieve a desired phenotype as well as mutation of multiple genes, required cumulatively to achieve a desired phenotype, in a cell or in a multi-cellular organism. The invention is also directed to methods of identifying one or more mutated genes, made by the methods of the invention, in cells and in multi-cellular organisms, by means of a tagging property provided by the insertional mutagen(s). The insertional mutagen thus allows identification of one or more genes that are mutated by insertion of an insertional mutagen. The invention is also directed to methods for correlating a phenotype with a gene by screening or selecting cells that have been mutated by an insertional mutagen incorporated into one or more genes in a cell and identifying the gene or genes causing the phenotype by means of a tagging property in one or more of the insertional mutagens. The invention is also directed to cells and multi-cellular organisms created by the methods of the invention and uses of the cells and multicellular organisms. The invention is also directed to libraries of cells created by the methods of the invention and uses of the libraries.

2. 发明申请

WO2003035834A2 COMPOSITIONS AND METHODS FOR MAKING MUTATIONS IN CELL LINES AND ANIMALS 审中-公开
标题翻译：用于在细胞系和动物中制备突变的组合物和方法
公开(公告)号：WO2003035834A2
公开(公告)日：2003-05-01
申请号：PCT/US2002/033714
申请日：2002-10-22
申请人： ATHERSYS, INC. , HARRINGTON, John, Joseph , JACKSON, Paul, David , JIANG, Li
发明人： HARRINGTON, John, Joseph , JACKSON, Paul, David , JIANG, Li
IPC分类号： C12N
CPC分类号： C12N15/102 , C12N15/01 , C12Q1/6897
摘要： The present invention is directed generally to reduction or inactivation of gene function or gene expression in cells in vitro and in multicellular organisms. The invention encompasses methods for mutating cells using a combination of mutagens, particularly wherein at least one mutagen is an insertional mutagen, to achieve homozygous gene mutation or mutation of multiple genes required cumulatively to achieve a phenotype to create knock-outs, knock-downs, and other modifications in the same cell. The invention is also directed to cells (and libraries thereof) and organisms created by the methods of the invention, including those in which at least one of the genes created by insertional mutagenesis is tagged by means of the insertion sequences thereby allowing identification of the mutated gene(s). The invention is also directed to libraries of mutated cells and their uses. The invention is also directed to methods of identifying mutations with methods of the invention, in cells (and libraries thereof) and organisms, bymeans of the insertional tag
摘要翻译：本发明总体上涉及体外和多细胞生物体中细胞中基因功能或基因表达的减少或失活。本发明包括使用诱变剂的组合来突变细胞的方法，特别是其中至少一种诱变剂是插入诱变剂，以实现累积需要的多个基因的纯合基因突变或突变以实现表型以产生敲除，敲除，和在同一个单元格中的其他修改。本发明还涉及通过本发明的方法产生的细胞（及其文库）和生物体，包括通过插入序列对至少一个通过插入诱变产生的基因进行标记的细胞，从而允许鉴定突变的基因（一个或多个）。本发明还涉及突变细胞的文库及其用途。本发明还涉及通过插入标记的方法在细胞（及其文库）和生物体中用本发明的方法鉴定突变的方法，

3. 发明申请

WO2003040324A2 METHODS FOR MUTATING GENES IN CELLS AND ANIMALS USING INSERTIONAL MUTAGENESIS 审中-公开
标题翻译：在细胞和动物中使用插入性突变体诱导基因的方法
公开(公告)号：WO2003040324A2
公开(公告)日：2003-05-15
申请号：PCT/US2002/035405
申请日：2002-11-04
申请人： ATHERSYS, INC. , HARRINGTON, John, Joseph , JACKSON, Paul, David , JIANG, Li
发明人： HARRINGTON, John, Joseph , JACKSON, Paul, David , JIANG, Li
IPC分类号： C12N
CPC分类号： C12N15/86 , A01K2217/075 , A01K2267/03 , C12N2740/10043 , C12N2800/60 , C12N2800/90 , C12N2840/203 , C12N2840/206 , C12N2840/44 , C12N2840/445
摘要： The present invention is in the fields of molecular biology, cell biology, and genetics. The invention is directed generally to mutating genes in cells in vitro and in multi-cellular organisms. The invention encompasses methods for mutating genes in cells using polynucleotides that act as insertional mutagens. Such methods are used to achieve mutation of a single gene to achieve a desired phenotype as well as mutation of multiple genes, required cumulatively to achieve a desired phenotype, in a cell or in a multi-cellular organism. The invention is also directed to methods of identifying one or more mutated genes, made by the methods of the invention, in cells and in multi-cellular organisms, by means of a tagging property provided by the insertional mutagen(s). The insertional mutagen thus allows identification of one or more genes that are mutated by insertion of an insertional mutagen. The invention is also directed to methods for correlating a phenotype with a gene by screening or selecting cells that have been mutated by an insertional mutagen incorporated into one or more genes in a cell and identifying the gene or genes causing the phenotype by means of a tagging property in one or more of the insertional mutagens. The invention is also directed to cells and multi-cellular organisms created by the methods of the invention and uses of the cells and multicellular organisms. The invention is also directed to libraries of cells created by the methods of the invention and uses of the libraries.
摘要翻译：本发明涉及分子生物学，细胞生物学和遗传学领域。本发明一般涉及体外和多细胞生物体中细胞中的突变基因。本发明包括使用充当插入诱变剂的多核苷酸突变细胞中的基因的方法。这样的方法用于实现单个基因的突变，以实现期望的表型以及在细胞或多细胞生物体中累积需要累积所需表型的多个基因的突变。本发明还涉及通过本发明的方法在细胞和多细胞生物体中通过插入诱变剂提供的标记特性鉴定一种或多种突变基因的方法。因此，插入型诱变剂允许鉴定通过插入插入型突变体而突变的一种或多种基因。本发明还涉及通过筛选或选择已经通过掺入细胞中的一个或多个基因的插入突变体突变的细胞并通过标记鉴定引起表型的基因或基因来将表型与基因相关联的方法属性在一个或多个插入诱变剂。本发明还涉及由本发明的方法和细胞和多细胞生物体的用途产生的细胞和多细胞生物体。本发明还涉及通过本发明的方法和图书馆的使用而创建的细胞库。

4. 发明授权

EP1451295B1 METHODS FOR MUTATING GENES IN CELLS AND ANIMALS USING INSERTIONAL MUTAGENESIS 有权
标题翻译：方法突变细胞和动物插入突变源基因的
公开(公告)号：EP1451295B1
公开(公告)日：2015-06-03
申请号：EP02780573.8
申请日：2002-11-04
申请人： Athersys, Inc.
发明人： HARRINGTON, John Joseph , JACKSON, Paul David , JIANG, Li
IPC分类号： C12N15/86
CPC分类号： C12N15/86 , A01K2217/075 , A01K2267/03 , C12N2740/10043 , C12N2800/60 , C12N2800/90 , C12N2840/203 , C12N2840/206 , C12N2840/44 , C12N2840/445

5. 发明申请

WO2013033086A3 METHOD AND COMPOSITION TO REDUCE GEL VISCOSITY IN THE INTERMEDIATE TEMPERATURE RANGE 审中-公开
公开(公告)号：WO2013033086A3
公开(公告)日：2013-03-07
申请号：PCT/US2012/052649
申请日：2012-08-28
申请人： SCHLUMBERGER CANADA LIMITED , SERVICES PETROLIERS SCHLUMBERGER , SCHLUMBERGER HOLDINGS LIMITED , SCHLUMBERGER TECHNOLOGY B.V. , PRAD RESEARCH AND DEVELOPMENT LIMITED , JIANG, Li , ALI, Syed A. , PARRIS, Michael D. , HUTCHINS, Richard D. , OGUNLAJA, Gbenga
发明人： JIANG, Li , ALI, Syed A. , PARRIS, Michael D. , HUTCHINS, Richard D. , OGUNLAJA, Gbenga
IPC分类号： C09K8/58 , C09K8/584
摘要： Methods for reducing a viscosity of a viscosified fluid include reacting, such as by depolymerizing and/or decomposing, a polymeric material of the viscosified fluid with a breaking agent including one or more organic peroxide breakers having a structural feature selected from a cyclic peroxide segment and/or multiple linear peroxide moieties per molecule. The methods of treating the subterranean are provided that include reacting, such as by depolymerizing and/or decomposing, a polymeric material of a viscosified treatment fluid with one or more organic peroxide breakers having a structural feature selected from cyclic peroxide segment and/or multiple linear peroxide moieties per molecule to facilitate breaking of the viscosified treatment fluid after the fracturing or treatment is finished.

6. 发明申请

WO2007034131A1 ELECTRO-CHEMICAL SENSOR 审中-公开
标题翻译：电化学传感器
公开(公告)号：WO2007034131A1
公开(公告)日：2007-03-29
申请号：PCT/GB2006/002935
申请日：2006-08-08
申请人： SCHLUMBERGER TECHNOLOGY B.V. , SCHLUMBERGER HOLDINGS LIMITED , SCHLUMBERGER CANADA LIMITED , SERVICES PETROLIERS SCHLUMBERGER , PRAD RESEARCH AND DEVELOPMENT N.V. , LAWRENCE, Nathan , ROBINSON, Kay, Louise , JIANG, Li , PAGELS, Markus , HACKETT, Simon
发明人： LAWRENCE, Nathan , ROBINSON, Kay, Louise , JIANG, Li , PAGELS, Markus , HACKETT, Simon
IPC分类号： G01N27/333 , G01N27/49 , E21B49/08
CPC分类号： E21B49/08 , E21B47/01 , G01N27/48
摘要： An electro-chemical sensor is described having two molecular redox systems one being sensitive the other insensitive to the species to be detected and both being covalently bound to a polymer and having a detector to detect relative shifts in the voltammograms of the two redox systems.
摘要翻译：描述了一种电化学传感器，其具有两个分子氧化还原系统，一个对待检测物质敏感的分子氧化还原体系，并且两者共价结合到聚合物上并具有检测器以检测两种氧化还原体系的伏安图的相对位移。

7. 发明申请

WO2004011929A1 METHODS AND APPARATUS FOR THE MEASUREMENT OF HYDROGEN SULPHIDE AND THIOLS IN FLUIDS 审中-公开
标题翻译：用于测量流体中硫化氢和硫酸的方法和装置
公开(公告)号：WO2004011929A1
公开(公告)日：2004-02-05
申请号：PCT/GB2003/002991
申请日：2003-07-10
申请人： SCHLUMBERGER TECHNOLOGY B.V. , PETROLEUM RESEARCH AND DEVELOPMENT N.V. , SCHLUMBERGER CANADA LIMITED , SCHLUMBERGER HOLDINGS LIMITED , SCHLUMBERGER OILFIELD ASSISTANCE LIMITED , SCHLUMBERGER OVERSEAS S.A. , SCHLUMBERGER SERVICES LIMITED , SCHLUMBERGER SURENCO S.A. , SERVICES PETROLIERS SCHLUMBERGER , JIANG, Li , JONES, Timothy, Gareth, John , LAWRENCE, Nathan , THOMPSON, Mary , COMPTON, Richard
发明人： JIANG, Li , JONES, Timothy, Gareth, John , LAWRENCE, Nathan , THOMPSON, Mary , COMPTON, Richard
IPC分类号： G01N33/00
CPC分类号： G01N33/0044 , E21B49/10 , G01N27/49
摘要： An electrochemical sensor for measuring the amount of hydrogen sulphide or thiols in a fluid comprises an electrically conductive porous electrode within which are dispersed a precursor and a reaction solution which together with the hydrogen sulphide or thiols a redox reaction resulting in an electrical current dependent upon the amount of hydrogen sulphide or thiols in the fluid. The reaction solution may be initially provided in the pores of the porous electrode, or derived in use from the fluid itself.
摘要翻译：用于测量流体中硫化氢或硫醇的量的电化学传感器包括导电多孔电极，其中分散有前体和反应溶液，其与硫化氢或硫醇一起进行氧化还原反应，导致电流取决于流体中的硫化氢或硫醇的量。反应溶液可以最初设置在多孔电极的孔中，或者在使用中从流体本身获得。

8. 发明申请

WO2012080978A2 COLD WEATHER COMPATIBLE CROSSLINKER SOLUTION 审中-公开
标题翻译：冷天气兼容的交联剂解决方案
公开(公告)号：WO2012080978A2
公开(公告)日：2012-06-21
申请号：PCT/IB2011/055712
申请日：2011-12-15
申请人： SCHLUMBERGER CANADA LIMITED , SERVICES PETROLIERS SCHLUMBERGER , SCHLUMBERGER HOLDINGS LIMITED , SCHLUMBERGER TECHNOLOGY B.V. , PRAD RESEARCH AND DEVELOPMENT LIMITED , PARRIS, Michael D. , JIANG, Li , WILLIAMSON, Don
发明人： PARRIS, Michael D. , JIANG, Li , WILLIAMSON, Don
IPC分类号： C11D7/26 , C11D7/22 , C09D5/16 , C08J7/04
CPC分类号： C09K8/685 , C08F8/00
摘要： Disclosed herein is a well treatment fluid comprising an aqueous solution comprising greater than or equal to about 1 wt% boron, at least 5 wt% of a co-solvent, and greater than or equal to about 5 wt% sodium hydroxide, potassium hydroxide, or a combination thereof, wherein the co-solvent comprises glycerol, ethylene glycol, propylene glycol, or a combination thereof. Methods of using the well treatment fluid are also disclosed.
摘要翻译：本文公开了一种井处理流体，其包含含有大于或等于约1重量％的硼，至少5重量％的助溶剂和大于或等于约5的水溶液 wt％氢氧化钠，氢氧化钾或其组合，其中所述助溶剂包含甘油，乙二醇，丙二醇或其组合。也公开了使用井处理流体的方法。

9. 发明申请

WO2010129892A2 METHOD AND APPARATUS FOR PROVIDING A HAPTIC FEEDBACK SHAPE-CHANGING DISPLAY 审中-公开
标题翻译：提供快速反馈形状变化显示的方法和装置
公开(公告)号：WO2010129892A2
公开(公告)日：2010-11-11
申请号：PCT/US2010/034078
申请日：2010-05-07
申请人： IMMERSION CORPORATION , GRANT, Danny, A. , MODARRES, Ali , CRUZ-HERNANDEZ, Juan, Manuel , JIANG, Li , BIRNBAUM, David, M. , PIERON, Remy , ULLRICH, Christopher, J. , LACROIX, Robert
发明人： GRANT, Danny, A. , MODARRES, Ali , CRUZ-HERNANDEZ, Juan, Manuel , JIANG, Li , BIRNBAUM, David, M. , PIERON, Remy , ULLRICH, Christopher, J. , LACROIX, Robert
IPC分类号： G09G5/00
CPC分类号： G06F3/016
摘要： A haptic device includes a processor, a communication module coupled to the processor for receiving a shape input, and a housing for housing the communication module and including a deformable portion. The deformable portion includes a deformation actuator, and the processor provides a signal to the deformation actuator in response to the shape input to deform the housing. The shape of other areas of the device may also change in response to the signal. The shape changes may provide haptic effects, provide information, provide ergonomic changes, provide additional functionality, etc., to a user of the device.
摘要翻译：触觉装置包括处理器，耦合到处理器的通信模块，用于接收形状输入，以及用于容纳通信模块并包括可变形部分的壳体。可变形部分包括变形致动器，并且处理器响应于形状输入而向变形致动器提供信号以使壳体变形。设备的其他区域的形状也可以响应于信号而改变。形状变化可以提供触觉效果，提供信息，向设备的用户提供人机工程学变化，提供附加功能等。

10. 发明申请

WO2010023517A2 DETECTING GAS COMPOUNDS FOR DOWNHOLE FLUID ANALYSIS 审中-公开
标题翻译：检测气体化合物进行井下流体分析
公开(公告)号：WO2010023517A2
公开(公告)日：2010-03-04
申请号：PCT/IB2009/006458
申请日：2009-08-06
申请人： SCHLUMBERGER TECHNOLOGY B.V. , SERVICES PETROLIERS SCHLUMBERGER , SCHLUMBERGER CANADA LIMITED , SCHLUMBERGER HOLDINGS LIMITED , PRAD RESEARCH AND DEVELOPMENT LIMITED , LAWRENCE, Jimmy , JONES, Timothy, G., J. , INDO, Kentaro , YAMATE, Tsutomu , MATSUMOTO, Noriyuki , TORIBIO, Michael , YOSHIUCHI, Hidetoshi , MEREDITH, Andrew , LAWRENCE, Nathan, S. , JIANG, Li , FUJISAWA, Go , MULLINS, Oliver, C.
发明人： LAWRENCE, Jimmy , JONES, Timothy, G., J. , INDO, Kentaro , YAMATE, Tsutomu , MATSUMOTO, Noriyuki , TORIBIO, Michael , YOSHIUCHI, Hidetoshi , MEREDITH, Andrew , LAWRENCE, Nathan, S. , JIANG, Li , FUJISAWA, Go , MULLINS, Oliver, C.
IPC分类号： E21B49/00 , E21B49/08
CPC分类号： E21B49/005 , E21B49/08
摘要： A gas separation and detection tool for performing in situ analysis of borehole fluid is described. A separation system such as a membrane is employed to separate one or more target gasses from the borehole fluid. The separated gas may be detected by reaction with another material or spectroscopy. When spectroscopy is employed, a test chamber defined by a housing is used to hold the gas undergoing test. Various techniques may be employed to protect the gas separation system from damage due to pressure differential. For example, a separation membrane may be integrated with layers that provide strength and rigidity. The integrated membrane separation may include one or more of a water impermeable layer, gas selective layer, inorganic base layer and metal support layer. The gas selective layer itself can also function as a water impermeable layer. The metal support layer enhances resistance to differential pressure. Alternatively, the chamber may be filled with a liquid or solid material.
摘要翻译：描述了用于执行井眼流体的原位分析的气体分离和检测工具。采用诸如膜的分离系统将一种或多种目标气体与井眼流体分离。分离的气体可以通过与另一种材料或光谱反应来检测。当采用光谱学时，使用由外壳限定的测试室来保持正在进行测试的气体。可以采用各种技术来保护气体分离系统免受压力差造成的损害。例如，分离膜可以与提供强度和刚性的层结合。集成膜分离可以包括不透水层，气体选择性层，无机基底层和金属支撑层中的一个或多个。气体选择层本身也可以用作不透水层。金属支撑层增强了对差压的抵抗力。或者，腔室可以填充液体或固体材料。

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