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1. 发明申请

WO2012140429A3 METHOD AND APPARATUS FOR THE ANALYSIS OF BIOLOGICAL SAMPLES 审中-公开
公开(公告)号：WO2012140429A3
公开(公告)日：2012-10-18
申请号：PCT/GB2012/050807
申请日：2012-04-12
申请人： MICROMASS UK LIMITED , WILLIAMS, Jonathan Paul , HUGHES, Christopher John , GREEN, Brian , VISSERS, Johannes Petrus Cornelis
发明人： WILLIAMS, Jonathan Paul , HUGHES, Christopher John , GREEN, Brian , VISSERS, Johannes Petrus Cornelis
IPC分类号： G01N33/72 , G01N33/68
摘要： A method for the detection and quantitation of analytes of interest and variants of the analyte of interest comprising the steps of: (i) providing a sample containing an analyte of interest; (ii) spiking sample with known amount of calibrant; (iii) performing an LCMS or LCMSMS analysis on the spiked sample to produce a data set; (iv) determining from said data set the relative quantity of analyte of interest to calibrant; (v) calculating the absolute quantity of the analyte of interest from said relative quantity of analyte of interest and said known amount of calibrant; (vi) searching for one or more previously identified candidate sequences for one or more known variants denoted by one or more specific peaks to identify the presence of said one or more known variants within the sample; (vii) determining the relative quantity or amount of said one or more variants to said analyte of interest; and (viii) calculating the absolute quantity or amount of any of said one or more variants present in the sample.

2. 发明申请

WO2016051191A1 MASS SPECTROMETRY FOR DETERMINING IF A MUTATED VARIANT OF A TARGET PROTEIN IS PRESENT IN A SAMPLE 审中-公开
标题翻译：如果一个目标蛋白质的变异的变体在样品中存在，则用于测定的质谱
公开(公告)号：WO2016051191A1
公开(公告)日：2016-04-07
申请号：PCT/GB2015/052881
申请日：2015-10-01
申请人： MICROMASS UK LIMITED
发明人： BROWN, Jeffery Mark , MORRIS, Michael Raymond , WILLIAMS, Jonathan , GREEN, Brian
IPC分类号： G01N33/68 , H01J49/00
CPC分类号： G01N33/6848 , G01N33/48 , G01N33/68 , G01N33/6818 , G01N33/6842 , G01N33/721 , H01J49/00 , H01J49/0045
摘要： A method of mass spectrometry is disclosed for determining if a mutated variant of a target protein is present in a sample. The method comprises subjecting the sample to fragmentation so as to cause said target protein to fragment to form second generation fragment ions, and then mass analysing these fragment ions to obtain spectral data. The method determines if a mutated variant is present in the sample by determining that an ion in the spectral data has a mass to charge ratio that differs from the mass to charge ratio of an ion that would be observed if said target protein was a normal unmutated version of said target protein, and by an amount that corresponds to a mass difference that would be caused by the target protein being a mutated variant of said target protein. This method of analysing second generation fragment ions if a rapid and efficiency method of analysing a sample.
摘要翻译：公开了用于确定样品中是否存在靶蛋白的突变型变体的质谱法。该方法包括使样品破碎以使所述靶蛋白片段化以形成第二代碎片离子，然后大量分析这些碎片离子以获得光谱数据。该方法通过确定光谱数据中的离子具有不同于如果所述靶蛋白是正常未突变的观察到的离子的质荷比不同，样品中是否存在突变的变体所述靶蛋白的版本，以及对应于由靶蛋白作为所述靶蛋白的突变变体引起的质量差的量。这种分析第二代碎片离子的方法，如果快速有效的分析样品的方法。

3. 发明授权

EP3201632B1 MASS SPECTROMETRY FOR DETERMINING IF A MUTATED VARIANT OF A TARGET PROTEIN IS PRESENT IN A SAMPLE 有权
公开(公告)号：EP3201632B1
公开(公告)日：2019-12-25
申请号：EP15784434.1
申请日：2015-10-01
申请人： Micromass UK Limited
发明人： BROWN, Jeffery Mark , MORRIS, Michael Raymond , WILLIAMS, Jonathan , GREEN, Brian
IPC分类号： G01N33/68 , G01N33/48 , G01N33/72 , H01J49/00

4. 发明公开

EP3201632A1 MASS SPECTROMETRY FOR DETERMINING IF A MUTATED VARIANT OF A TARGET PROTEIN IS PRESENT IN A SAMPLE 有权
标题翻译：用于确定样品中突变的目标蛋白的质谱的质谱分析法
公开(公告)号：EP3201632A1
公开(公告)日：2017-08-09
申请号：EP15784434.1
申请日：2015-10-01
申请人： Micromass UK Limited
发明人： BROWN, Jeffery Mark , MORRIS, Michael Raymond , WILLIAMS, Jonathan , GREEN, Brian
IPC分类号： G01N33/68 , H01J49/00
摘要： A method of mass spectrometry is disclosed for determining if a mutated variant of a target protein is present in a sample. The method comprises subjecting the sample to fragmentation so as to cause said target protein to fragment to form second generation fragment ions, and then mass analysing these fragment ions to obtain spectral data. The method determines if a mutated variant is present in the sample by determining that an ion in the spectral data has a mass to charge ratio that differs from the mass to charge ratio of an ion that would be observed if said target protein was a normal unmutated version of said target protein, and by an amount that corresponds to a mass difference that would be caused by the target protein being a mutated variant of said target protein. This method of analysing second generation fragment ions if a rapid and efficiency method of analysing a sample.
摘要翻译：公开了一种用于确定样品中是否存在靶蛋白的突变变体的质谱方法。该方法包括对样品进行碎裂以使所述目标蛋白质片段化以形成第二代碎片离子，然后对这些碎片离子进行质量分析以获得光谱数据。该方法通过确定谱数据中的离子具有与如果所述靶蛋白是正常未突变的情况下将观察到的离子的质荷比不同的质荷比的比例来确定突变变体是否存在于样品中所述目标蛋白质的版本和对应于作为所述目标蛋白质的突变变体的靶蛋白质可能引起的质量差异的量。还公开了应用于血红蛋白样品的电子捕获解离或电子转移解离电离技术，其中分析初级或次级离子以鉴定血红蛋白突变。

5. 发明公开

EP2697655A2 METHOD AND APPARATUS FOR THE ANALYSIS OF BIOLOGICAL SAMPLES 审中-公开
标题翻译：方法和设备用于生物样品分析
公开(公告)号：EP2697655A2
公开(公告)日：2014-02-19
申请号：EP12720940.1
申请日：2012-04-12
申请人： Micromass UK Limited
发明人： WILLIAMS, Jonathan Paul , HUGHES, Christopher John , GREEN, Brian , VISSERS, Johannes Petrus Cornelis
IPC分类号： G01N33/72 , G01N33/68
CPC分类号： G01N33/721 , G01N30/7233 , G01N30/8675 , G01N33/6848 , G01N33/72 , G01N2030/045 , G01N2030/8822 , G01N2560/00
摘要： A method for the detection and quantitation of analytes of interest and variants of the analyte of interest comprising the steps of: (i) providing a sample containing an analyte of interest; (ii) spiking sample with known amount of calibrant; (iii) performing an LCMS or LCMSMS analysis on the spiked sample to produce a data set; (iv) determining from said data set the relative quantity of analyte of interest to calibrant; (v) calculating the absolute quantity of the analyte of interest from said relative quantity of analyte of interest and said known amount of calibrant; (vi) searching for one or more previously identified candidate sequences for one or more known variants denoted by one or more specific peaks to identify the presence of said one or more known variants within the sample; (vii) determining the relative quantity or amount of said one or more variants to said analyte of interest; and (viii) calculating the absolute quantity or amount of any of said one or more variants present in the sample.

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