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1. 发明专利

AU2010295637A1 Generation of high polyhydroxybutrate producing oilseeds 未知
公开(公告)号：AU2010295637A1
公开(公告)日：2012-04-26
申请号：AU2010295637
申请日：2010-09-15
申请人： METABOLIX INC , DONALD DANFORTH PLANT SCI CT
发明人： PATTERSON NII , TANG JIHONG , CAHOON EDGAR BENJAMIN , JAWORSKI JAN G , YANG WENYU , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H1/02 , C12N15/82
摘要： Transgenic oilseed plants, plant material, plant cells, and genetic constructs for synthesis of polyhydroxyalkanoates ("PHA") are provided. In a preferred embodiment, the transgenic oilseed plants synthesize (poly)3- hydroxybutyrate ("PHB") in the seed. Genes utilized include phaA,phaB, phaC, all of which are known in the art. The genes can be introduced in the plant, plant tissue, or plant cell using conventional plant molecular biology techniques.

2. 发明专利

CA2773703A1 GENERATION OF HIGH POLYHYDROXYBUTRATE PRODUCING OILSEEDS 未知
公开(公告)号：CA2773703A1
公开(公告)日：2011-03-24
申请号：CA2773703
申请日：2010-09-15
申请人： METABOLIX INC , DONALD DANFORTH PLANT SCI CT
发明人： PATTERSON NII , TANG JIHONG , CAHOON EDGAR BENJAMIN , JAWORSKI JAN G , YANG WENYU , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H1/02 , C12N15/82
摘要： Transgenic oilseed plants, plant material, plant cells, and genetic constructs for synthesis of polyhydroxyalkanoates ("PHA") are provided. In a preferred embodiment, the transgenic oilseed plants synthesize (poly)3- hydroxybutyrate ("PHB") in the seed. Genes utilized include phaA,phaB, phaC, all of which are known in the art. The genes can be introduced in the plant, plant tissue, or plant cell using conventional plant molecular biology techniques.

3. 发明专利

JP2007125043A Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways 审中-公开
标题翻译：从脂肪酸生产途径生产中链长度聚羟基烷酸
公开(公告)号：JP2007125043A
公开(公告)日：2007-05-24
申请号：JP2007035383
申请日：2007-02-15
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： AQUIN STEPHANIE , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H5/00 , C12Q1/44 , A01H1/00 , C12N1/21 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/09 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12P7/64 , C12Q1/02 , C12Q1/25 , C12Q1/48
CPC分类号： C12N9/93 , C12N9/00 , C12N9/10 , C12N9/1029 , C12N9/16 , C12N15/8243
摘要： PROBLEM TO BE SOLVED: To express an acyl CoA synthetase, a PHA synthase and a PhaG in an organism to produce PHA. SOLUTION: A method for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, is provided. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase (with substrate specificity for medium chain length 3-hydroxy fatty acids), and a medium chain length PHA synthase, is provided. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：在生物体中表达酰基辅酶A合成酶，PHA合酶和PhaG以产生PHA。解决方案：提供了使用3-羟基酰基ACP硫酯酶，PHA合成酶和酰基辅酶A合成酶从脂肪酸生物合成途径制备聚羟基链烷酸酯（PHA）的方法。使用具有3-羟基酰基ACP硫酯酶（酰基辅酶A合成酶（具有中等链长度3-羟基脂肪酸的底物特异性））的酶的非天然细菌PHA生产者和植物中的脂肪酸生物合成途径的PHA生产的方法酸）和中链长度PHA合酶。版权所有（C）2007，JPO＆INPIT

4. 发明专利

JP2008194049A Production of medium chain length polyhydroxyalkanoate from fatty acid biosynthetic pathway 审中-公开
标题翻译：从脂肪酸生物路线生产中链长度聚羟基烷酸
公开(公告)号：JP2008194049A
公开(公告)日：2008-08-28
申请号：JP2008061883
申请日：2008-03-11
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： AQUIN STEPHANIE , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H5/00 , C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12P7/62 , C12Q1/02 , C12Q1/25 , C12Q1/44 , C12Q1/48
CPC分类号： C12N9/93 , C12N9/00 , C12N9/10 , C12N9/1029 , C12N9/16 , C12N15/8243
摘要： PROBLEM TO BE SOLVED: To express PhaG together with acyl CoA synthetase and PHA synthase in organisms in order to carry out production of PHA. SOLUTION: A method for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, has been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants by using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase (with substrate specificity for medium chain length 3-hydroxy fatty acids) and a medium chain length PHA synthase has been developed. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：与生物体中的酰基辅酶A合成酶和PHA合酶一起表达PhaG，以进行PHA的生产。解决方案：已经开发了使用3-羟基酰基ACP硫酯酶，PHA合成酶和酰基辅酶A合成酶从脂肪酸生物合成途径制备聚羟基链烷酸酯（PHA）的方法。通过使用具有3-羟基酰基ACP硫酯酶（酰基辅酶A合成酶）（具有中链长度3-羟基的底物特异性）的催化活性的酶，在非天然细菌PHA生产者和植物中通过脂肪酸生物合成途径产生PHA的方法脂肪酸）和中链长度的PHA合酶。版权所有（C）2008，JPO＆INPIT

5. 发明专利

JP2007222185A Production of medium chain length polyhydroxyalkanoate from fatty acid biosynthetic pathway 有权
标题翻译：从脂肪酸生物路线生产中链长度聚羟基烷酸
公开(公告)号：JP2007222185A
公开(公告)日：2007-09-06
申请号：JP2007153418
申请日：2007-06-08
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： AQUIN STEPHANIE , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H5/00 , C12Q1/44 , A01H1/00 , C12N1/21 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/09 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12Q1/02 , C12Q1/25 , C12Q1/48
CPC分类号： C12N9/93 , C12N9/00 , C12N9/10 , C12N9/1029 , C12N9/16 , C12N15/8243
摘要： PROBLEM TO BE SOLVED: To develop a PhaG with an acyl CoA synthetase and a PHA synthase in an organism so as to produce PHA. SOLUTION: Methods for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways by using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, have been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants by using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase (having substrate specificity for medium chain length 3-hydroxy fatty acids), and a medium chain length PHA synthase, has been developed. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：在生物体中开发具有酰基辅酶A合成酶和PHA合成酶的PhaG，以产生PHA。解决方案：已经开发了通过使用3-羟基酰基ACP硫酯酶，PHA合成酶和酰基辅酶A合成酶从脂肪酸生物合成途径制备聚羟基链烷酸酯（PHA）的方法。通过使用具有3-羟基酰基ACP硫酯酶的催化活性的酶，酰基辅酶A合成酶（具有中链长度3-羟基的底物特异性），使非天然细菌PHA生产者和植物中脂肪酸生物合成途径产生PHA的方法脂肪酸）和中链长度PHA合成酶。版权所有（C）2007，JPO＆INPIT

6. 发明申请

WO0240690A9 PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM FATTY ACID BIOSYNTHETIC PATHWAYS 审中-公开
标题翻译：从脂肪酸生物合成途径生产中链长度聚羟基烷酸酯
公开(公告)号：WO0240690A9
公开(公告)日：2003-11-20
申请号：PCT/US0143686
申请日：2001-11-16
申请人： METABOLIX INC
发明人： AQUIN STEPHANIE , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H5/00 , C12N1/21 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/09 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12Q1/02 , C12Q1/25 , C12Q1/44 , C12Q1/48 , C12N15/52 , C12N15/70
CPC分类号： C12N9/93 , C12N9/00 , C12N9/10 , C12N9/1029 , C12N9/16 , C12N15/8243
摘要： Methods for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, have been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase with substrate specificity for medium chain length 3-hydroxy fatty acids, and a medium chain length PHA synthase, has been developed. Acyl CoA synthetase activity can be supplied either by the endogenous acyl CoA synthetase of the host organism, when sufficiently expressed, or the host organism's activity can be supplemented by the expression of a recombinant acyl CoA synthetase gene. New strategies are described for plant based PHA production in the chloroplasts, cytosol, and peroxisomes of biomass crops as well as the plastids, cytosol, and peroxisomes of oil seed crops.
摘要翻译：已经开发了使用3-羟基酰基ACP硫酯酶，PHA合酶和酰基CoA合成酶从脂肪酸生物合成途径生产聚羟基链烷酸酯（PHA）的方法。使用具有催化活性的3-羟基酰基ACP硫酯酶的酶，在非天然细菌PHA生产者和植物中从脂肪酸生物合成途径产生PHA的方法，所述酰基CoA合成酶对中等链长3-羟基脂肪酸具有底物特异性，和一个中等链长的PHA合成酶。当充分表达时，酰基辅酶A合成酶活性可以通过宿主生物体的内源性酰基CoA合成酶来提供，或者宿主生物体的活性可以通过表达重组酰基CoA合成酶基因来补充。新的策略描述了基于植物的生物量作物的叶绿体，细胞质和过氧化物酶体以及油籽作物的质体，细胞质和过氧化物酶体的PHA生产。

7. 发明申请

WO0240690A2 PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM FATTY ACID BIOSYNTHETIC PATHWAYS 审中-公开
标题翻译：从脂肪酸生产途径生产中链长度聚羟基烷酸
公开(公告)号：WO0240690A2
公开(公告)日：2002-05-23
申请号：PCT/US0143686
申请日：2001-11-16
申请人： METABOLIX INC
发明人： AQUIN STEPHANIE , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H5/00 , C12N1/21 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/09 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12Q1/02 , C12Q1/25 , C12Q1/44 , C12Q1/48
CPC分类号： C12N9/93 , C12N9/00 , C12N9/10 , C12N9/1029 , C12N9/16 , C12N15/8243
摘要： Methods for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, have been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase with substrate specificity for medium chain length 3-hydroxy fatty acids, and a medium chain length PHA synthase, has been developed. Acyl CoA synthetase activity can be supplied either by the endogenous acyl CoA synthetase of the host organism, when sufficiently expressed, or the host organism's activity can be supplemented by the expression of a recombinant acyl CoA synthetase gene. New strategies are described for plant based PHA production in the chloroplasts, cytosol, and peroxisomes of biomass crops as well as the plastids, cytosol, and peroxisomes of oil seed crops.
摘要翻译：已经开发了使用3-羟基酰基ACP硫酯酶，PHA合成酶和酰基辅酶A合成酶从脂肪酸生物合成途径生产聚羟基链烷酸酯（PHA）的方法。使用具有3-羟基酰基ACP硫酯酶的催化活性的酶，非天然细菌PHA生产者和植物中的脂肪酸生物合成途径的PHA生产的方法，具有对于中链长度3-羟基脂肪酸具有底物特异性的酰基辅酶A合成酶，和中链长度PHA合酶。酰基辅酶A合成酶活性可以通过宿主生物体的内源酰基辅酶A合成酶提供，当被充分表达时，或者宿主生物的活性可以通过重组酰基辅酶A合成酶基因的表达补充。描述了用于生物量作物的叶绿体，细胞溶质和过氧化物酶体以及油料种子作物的质体，细胞溶质和过氧化物酶体的基于植物的PHA生产的新策略。

8. 发明申请

WO0159091A3 MULTI-GENE EXPRESSION CONSTRUCTS CONTAINING MODIFIED INTEINS 审中-公开
标题翻译：含有改性浓度的多基因表达构建体
公开(公告)号：WO0159091A3
公开(公告)日：2001-12-20
申请号：PCT/US0104254
申请日：2001-02-09
申请人： METABOLIX INC
发明人： SNELL KRISTI D
IPC分类号： C12N15/82 , C12P7/62 , C12N15/63
CPC分类号： C12N15/8216 , C12P7/625
摘要： Methods and constructs for the introduction of multiple genes into plants using a single transformation event are described. Constructs contain a single 5' promoter operably linked to DNA encoding a modified intein splicing unit. The splicing unit is expressed as a polyprotein and consists of a first protein fused to an intein fused to a second protein. The splicing unit has been engineered to promote excision of all non-essential components in the polyprotein but prevent the ligation reactions normally associated with protein splicing. Additional genetic elements encoding inteins and additional proteins can be fused in frame to the 5'-terminus of the coding regions for the second protein to form a construct for expression of more than two proteins. A single 3' termination sequence, such as a polyadenylation sequence when the construct is to be expressed in eucaryotic cells, follows the last coding sequence. These methods and constructs are particularly useful for creating plants with stacked input traits, illustrated by glyphosate tolerant plants producing BT toxin, and/or value added products, illustrated by the production of polyhydroxyalkanoates in plants.
摘要翻译：描述了使用单一转化事件将多个基因引入植物的方法和构建体。构建体包含与编码修饰的内含肽剪接单元的DNA可操作地连接的单个5'启动子。剪接单元表示为多聚蛋白，由融合至第二蛋白质的内含肽融合的第一个蛋白质组成。剪接单元已被设计用于促进多蛋白中所有非必需组分的切除，但是防止通常与蛋白质剪接相关的连接反应。编码内含肽和其他蛋白质的其他遗传元件可以与第二个蛋白质的编码区的5'端融合，形成表达两种以上蛋白质的构建体。当构建体在真核细胞中表达时，单个3'终止序列，例如聚腺苷酸化序列，遵循最后的编码序列。这些方法和结构对于产生具有堆叠输入性状的植物特别有用，由产生BT毒素的草甘膦耐受植物和/或增值产物说明，其通过在植物中生产聚羟基链烷酸酯来说明。

9. 发明专利

ES2336641T3 CONSTRUCTOS DE EXPRESION MULTIGENICOS QUE CONTIENEN INTEINAS MODIFICADAS. 未知
公开(公告)号：ES2336641T3
公开(公告)日：2010-04-15
申请号：ES01909045
申请日：2001-02-09
申请人： METABOLIX INC
发明人： SNELL KRISTI D
IPC分类号： C12N15/82 , C12N15/63 , C12P7/62
摘要： Un constructo de DNA para expresión de productos de genes múltiples en una célula eucariota, comprendiendo el constructo de DNA una casete que está constituida por las características que se muestran, en la dirección 5'' a 3'', en la fórmula siguiente: (i)-(ii)-[(iii)-(iv)]n-(v) (a) en la cual: (i) es un promotor simple en el extremo 5'' de la casete, (ii) es una secuencia de DNA que codifica una proteína exteína N-terminal; (iii) es una secuencia de DNA que codifica una inteína, (iv) es una secuencia de DNA que codifica una proteína exteína C-terminal, y (v) es una región no codificante 3'' que es operativa como secuencia de terminación de la transcripción, y comprende una señal de poliadenilación, (b) en la cual n es un número entero de uno o más, y (c) en la cual (ii)-[(iii)-(iv)]n, como se muestra en la fórmula anterior codifican juntos una unidad de remodelación de inteína modificada que se expresa como una sola poliproteína precursora y que cataliza la escisión de las exteínas de la o de cada una de las inteínas, y previene la ligación de las exteínas.

10. 发明专利

AU3646402A Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways 未知
公开(公告)号：AU3646402A
公开(公告)日：2002-05-27
申请号：AU3646402
申请日：2001-11-16
申请人： METABOLIX INC
发明人： AQUIN STEPHANIE , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H5/00 , C12N1/21 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/09 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12Q1/02 , C12Q1/25 , C12Q1/44 , C12Q1/48

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