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1. 发明申请

WO2007022348A3 AMPLIFICATION OF CELL POLULATIONS FROM EMBRYONIC STEM CELLS 审中-公开
公开(公告)号：WO2007022348A3
公开(公告)日：2007-02-22
申请号：PCT/US2006/032132
申请日：2006-08-17
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , KARP, Jeffrey, J. , FERREIRA, Lino, S. , KHADEMHOSSEINI, Ali , LANGER, Robert
发明人： KARP, Jeffrey, J. , FERREIRA, Lino, S. , KHADEMHOSSEINI, Ali , LANGER, Robert
IPC分类号： C12N5/02
摘要： Culturing embryonic stem cells without the use of embryoid bodies leads to an increase in the frequency of predetermined cell types.

2. 发明申请

WO2008131301A1 SURFACES, METHODS AND DEVICES EMPLOYING CELL ROLLING 审中-公开
标题翻译：表面，方法和使用细胞滚动的装置
公开(公告)号：WO2008131301A1
公开(公告)日：2008-10-30
申请号：PCT/US2008/060934
申请日：2008-04-18
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , KARP, Jeffrey, M. , MOORE, Michael, J. , KHADEMHOSSEINI, Ali , LANGER, Robert, S. , HONG, Seungpyo
发明人： KARP, Jeffrey, M. , MOORE, Michael, J. , KHADEMHOSSEINI, Ali , LANGER, Robert, S. , HONG, Seungpyo
IPC分类号： A61K38/36
CPC分类号： A61L27/3808 , A61L27/34 , A61L27/507 , A61L27/54 , A61L27/58 , A61L31/005 , A61L31/10 , A61L31/148 , A61L31/16 , A61L2300/25 , A61L2300/252 , A61L2400/06
摘要： In various aspects, the present invention provides surfaces and materials for cell rolling applications, methods of making such surfaces and materials, and devices having such surfaces and materials. In some embodiments, the present invention provides surfaces with at least partial coatings of an ordered layer of cell adhesion molecules, or fragments, analogs, or modifications thereof, covalently bound to the surface of the substrate through an immobilization moiety. In some embodiments, the layer of a cell adhesion molecules further comprises a cell modifying ligand that can be targeted, e.g ., to one or more specific cell types.
摘要翻译：在各个方面，本发明提供了用于电池轧制应用的表面和材料，制造这种表面和材料的方法，以及具有这种表面和材料的装置。在一些实施方案中，本发明提供具有细胞粘附分子或其片段，类似物或修饰的有序层的至少部分涂层的表面，其通过固定部分共价结合到所述底物的表面。在一些实施方案中，细胞粘附分子的层还包含可靶向例如一种或多种特定细胞类型的细胞修饰配体。

3. 发明申请

WO2011035185A2 A MICROFLUIDIC DEVICE AND USES THEREOF 审中-公开
标题翻译：微流体装置及其用途
公开(公告)号：WO2011035185A2
公开(公告)日：2011-03-24
申请号：PCT/US2010/049372
申请日：2010-09-17
申请人： THE BRIGHAM AND WOMEN'S HOSPITAL, INC. , KHADEMHOSSEINI, Ali , KHABIRY, Masoud , CHUNG, Bong Geun , HANCOCK, Matthew , DU, Yanan
发明人： KHADEMHOSSEINI, Ali , KHABIRY, Masoud , CHUNG, Bong Geun , HANCOCK, Matthew , DU, Yanan
IPC分类号： G01N33/48 , C12Q1/02 , C12M1/34 , C12M3/00 , B81C1/00 , G01N35/00
CPC分类号： B01L3/502761 , B01L2300/0636 , B01L2300/0809 , B01L2300/087 , B01L2300/0887 , C12M23/16
摘要： Microfluidic devices and methods for using microfluidic devices for immobilizing cells, e.g., mammalian cells, suspended in a flowing fluid include selecting the geometry and dimensions of one or more multi-surface cavities disposed therein, and/or the flow direction of a cell-containing fluid therein. The cavity can comprise an upper and lower surface, and cells can be captured on either surfaces located at two different depths within a cavity. The invention includes methods for generating co-cultures of different cell populations inside a multi-surface cavity disposed within microfluidic devices as disclosed herein.
摘要翻译：用于使用微流体装置来固定悬浮在流动流体中的细胞（例如哺乳动物细胞）的微流体装置和方法包括选择设置在其中的一个或多个多表面空腔的几何形状和尺寸，和/或其中含有细胞的流体的流动方向。腔体可以包括上表面和下表面，并且细胞可以被捕获在位于腔体内两个不同深度的任一表面上。本发明包括如本文所公开的用于在设置在微流体装置内的多表面腔内产生不同细胞群的共培养物的方法。

4. 发明申请

WO2009014546A2 PARYLENE-C STENCILS 审中-公开
公开(公告)号：WO2009014546A2
公开(公告)日：2009-01-29
申请号：PCT/US2007/081553
申请日：2007-10-16
申请人： THE BRIGHAM AND WOMEN'S HOSPITAL, INC. , KHADEMHOSSEINI, Ali , RAJALINGAM, Bimalraj
发明人： KHADEMHOSSEINI, Ali , RAJALINGAM, Bimalraj
IPC分类号： B32B33/00 , B05D1/32
CPC分类号： C12N5/0068 , B41M1/12 , C12N5/0606 , C12N2502/13 , C12N2502/28 , C12N2533/52 , C12N2533/54 , C12N2533/70 , C12N2535/10 , G03F7/12 , Y10T428/1476
摘要： A reuseable microfabrication stencil is described that can be reversibly sealed on various substrates to pattern biomolecules and biomaterials such as proteins and cells. The stencil may be used for the generation of both static and dynamic co-cultures, and cell aggregates. A multilayer stencil is described that can be used in biological patterning and used to create static and dynamic co-cultures and cell aggregates. Processes for producing and using the microfabrication stencils are also described.
摘要翻译：描述了一种可重复使用的微制造模板，其可以可逆地密封在各种基材上以图案化生物分子和生物材料，例如蛋白质和细胞。模板可用于产生静态和动态共培养物和细胞聚集体。描述了可用于生物图案化并用于创建静态和动态共培养物和细胞聚集体的多层模板。还介绍了生产和使用微制造模板的流程。

5. 发明公开

EP2148696A1 SURFACES, METHODS AND DEVICES EMPLOYING CELL ROLLING 审中-公开
标题翻译：表面上，方法和设备能否与电芯绕组
公开(公告)号：EP2148696A1
公开(公告)日：2010-02-03
申请号：EP08746368.3
申请日：2008-04-18
申请人： Massachusetts Institute of Technology
发明人： KARP, Jeffrey, M. , MOORE, Michael, J. , KHADEMHOSSEINI, Ali , LANGER, Robert, S. , HONG, Seungpyo
IPC分类号： A61K38/36
CPC分类号： A61L27/3808 , A61L27/34 , A61L27/507 , A61L27/54 , A61L27/58 , A61L31/005 , A61L31/10 , A61L31/148 , A61L31/16 , A61L2300/25 , A61L2300/252 , A61L2400/06
摘要： In various aspects, the present invention provides surfaces and materials for cell rolling applications, methods of making such surfaces and materials, and devices having such surfaces and materials. In some embodiments, the present invention provides surfaces with at least partial coatings of an ordered layer of cell adhesion molecules, or fragments, analogs, or modifications thereof, covalently bound to the surface of the substrate through an immobilization moiety. In some embodiments, the layer of a cell adhesion molecules further comprises a cell modifying ligand that can be targeted, e.g., to one or more specific cell types.

6. 发明申请

WO2010057078A2 METHOD AND SYSTEM FOR GENERATING SPATIALLY AND TEMPORALLY CONTROLLABLE CONCENTRATION GRADIENTS 审中-公开
标题翻译：用于产生空间和时间可控浓度梯度的方法和系统
公开(公告)号：WO2010057078A2
公开(公告)日：2010-05-20
申请号：PCT/US2009/064555
申请日：2009-11-16
申请人： THE BRIGHAM AND WOMEN'S HOSPITAL, INC. , KHADEMHOSSEINI, Ali , DU, Yanan
发明人： KHADEMHOSSEINI, Ali , DU, Yanan
IPC分类号： G01N35/08 , G01N33/48 , C12Q1/00
CPC分类号： G01N30/34 , B01L3/50273 , B01L3/502776 , B01L2300/0816 , G01N1/405 , G01N2001/4088 , G01N2030/342 , Y10T436/2575 , G01N30/6095
摘要： The ability to rapidly generate concentration gradients of diffusible molecules has important applications in many chemical and biological studies. The present invention is directed to methods and systems for generating spatially and temporally controllable concentration gradients of molecules (i.e. proteins or toxins) in a portable microfluidic device. The formation of the concentration gradients can be initiated by an induced forward flow and further optimized during an induced backward flow. The forward and backward flows can be either passively induced and/or actively pumped. The centimeter-length gradients along the microfluidic channel can be spatially and temporally controlled by the backward flow. The gradient profile was stabilized by stopping the flow. In one example, a stabilized concentration gradient of a cardiac toxin, Alpha-cypermethrin, generated according to the invention was used to test the response of HL-1 cardiac cells in the microfluidic device, which correlated with toxicity data obtained from multi-well plates. The invention can be useful for biological and chemical processes that require rapid generation of concentration gradients in a portable microfluidic device.
摘要翻译：快速产生扩散分子的浓度梯度的能力在许多化学和生物学研究中具有重要的应用。本发明涉及用于在便携式微流体装置中产生分子（即蛋白质或毒素）的空间和时间可控浓度梯度的方法和系统。浓度梯度的形成可以通过诱导的正向流引发并且在诱导的逆流期间进一步优化。前向和后向流可以被动地被诱导和/或被主动泵送。沿着微流体通道的厘米长度梯度可以由反向流动在空间和时间上控制。通过停止流动来稳定梯度分布。在一个实例中，使用根据本发明产生的心脏毒素α-氯氰菊酯的稳定化浓度梯度来测试微流体装置中HL-1心脏细胞的反应，其与从多孔板获得的毒性数据相关。本发明可用于需要在便携式微流体装置中快速产生浓度梯度的生物和化学过程。

7. 发明申请

WO2011035177A2 HIGH-THROUGHPUT SCREENING 审中-公开
标题翻译：高通量筛选
公开(公告)号：WO2011035177A2
公开(公告)日：2011-03-24
申请号：PCT/US2010/049362
申请日：2010-09-17
申请人： THE BRIGHAM AND WOMEN'S HOSPITAL, INC. , KHADEMHOSSEINI, Ali , WU, Jinhui , WHEELDON, Ian, Richard
发明人： KHADEMHOSSEINI, Ali , WU, Jinhui , WHEELDON, Ian, Richard
IPC分类号： G01N33/50 , G01N33/48 , G01N35/02
CPC分类号： G01N33/5008 , B01J2219/00317 , B01J2219/00509 , B01J2219/00531 , B01J2219/00702 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , B01J2219/00743
摘要： The invention provides a high throughput screening device having a microwell microarray and a printed microarray of test compound deposits. The layout of the test compound deposits matches the layout of the microwells such that each deposit addresses a single microwell when the two arrays are contacted with each other. The invention further provides methods of screening test compounds for biological activity using the device. The invention also provides methods of fabricating the device.
摘要翻译：本发明提供了具有微孔微阵列和测试化合物沉积物的印刷微阵列的高通量筛选装置。测试化合物沉积物的布局与微孔的布局匹配，使得当两个阵列彼此接触时每个沉积物处理单个微孔。本发明还提供使用该装置筛选测试化合物的生物活性的方法。本发明还提供了制造该装置的方法。

8. 发明申请

WO2008070345A2 CONTROLLED RELEASE OF A CURING AGENT FOR THE GENERATION OF MICROSTRUCTURES 审中-公开
标题翻译：用于生成微结构的固化剂的控制释放
公开(公告)号：WO2008070345A2
公开(公告)日：2008-06-12
申请号：PCT/US2007/082690
申请日：2007-10-26
申请人： THE BRIGHAM AND WOMEN'S HOSPITAL, INC. , KHADEMHOSSEINI, Ali , FRANZESI, Giovanni, Talei , NI, Bin , LING, Yibo
发明人： KHADEMHOSSEINI, Ali , FRANZESI, Giovanni, Talei , NI, Bin , LING, Yibo
IPC分类号： B29C41/20 , B29C41/22 , B32B7/00
CPC分类号： A61K9/1652 , A61K9/0097 , A61K9/1694 , A61L27/38 , A61L27/52 , A61L27/54 , A61L2300/602 , B81C1/0046 , C08L5/04 , Y10T428/2991
摘要： A controlled release fabrication process includes shaping a precursor material into a desired configuration with a mold, supplying a curing agent from the mold to the precursor material, curing the precursor material to form a mechanically stable structure, and releasing the mechanically stable structure from the mold. Mechanically stable structures including microfabrication articles produced using the process are also described.
摘要翻译：控释制造方法包括用模具将前体材料成型为期望的构型，将固化剂从模具供应到前体材料，固化前体材料以形成机械稳定的结构，并将机械稳定的结构从模具中释放。还描述了使用该方法生产的包括微细加工制品的机械稳定结构。

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