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1. 发明授权

US6057493A Plasmids, plants and plant cells expressing anti-sense patatin and anti-sense ADP-glucose pyrophosphorylase sequences 失效
标题翻译：表达反义patatin和反义ADP-葡萄糖焦磷酸化酶序列的质粒，植物和植物细胞
公开(公告)号：US6057493A
公开(公告)日：2000-05-02
申请号：US177719
申请日：1994-01-05
申请人： Lothar Willmitzer , Uwe Sonnewald , Rainer Hofgen , Jens Kossmann , Bernd Muller
发明人： Lothar Willmitzer , Uwe Sonnewald , Rainer Hofgen , Jens Kossmann , Bernd Muller
IPC分类号： A01H1/00 , A01H5/00 , A23K10/35 , A23K20/147 , A23L1/09 , A23L1/216 , C07K14/415 , C12N1/21 , C12N5/10 , C12N5/14 , C12N9/12 , C12N15/09 , C12N15/113 , C12N15/29 , C12N15/54 , C12N15/82 , C12R1/19 , C12N5/04
CPC分类号： C12N15/113 , A23K10/35 , A23K20/147 , C07K14/415 , C12N15/8245 , C12N9/1241 , C12N2310/111 , C12N2310/321
摘要： Plasmids containing DNA sequences which when inserted into the genome of a plant modify the carbohydrate or protein concentration and the carbohydrate or protein composition, and plant cells and plants that contain these plasmids.DNA sequences located in plasmids reduce ADP-glucose-pyrophosphorylase activity in the plant and thereby the starch concentration, while at the same time increase the mono- and oligosaccharide concentration. Other DNA sequences in plasmids reduce or increase the protein concentration. Plants that contain these plasmids are suitable inter alia for the extraction of sugar or as protein-enriched food or fodder.
摘要翻译：含有DNA序列的质粒当插入到植物基因组中时，修饰碳水化合物或蛋白质浓度和碳水化合物或蛋白质组成，以及含有这些质粒的植物细胞和植物。位于质粒中的DNA序列降低植物中的ADP-葡萄糖 - 焦磷酸化酶活性，从而降低淀粉浓度，同时增加单糖和寡糖浓度。质粒中的其他DNA序列降低或增加蛋白质浓度。含有这些质粒的植物尤其适用于提取糖或作为富含蛋白质的食物或饲料。

2. 发明授权

US06215042B1 Plasmids containing DNA-sequences that cause changes in the carbohydrate concentration and carbohydrate composition in plants, as well as plant cells and plants containing these plasmids 失效
标题翻译：含有导致植物中碳水化合物浓度和碳水化合物组成变化的DNA序列的质粒以及含有这些质粒的植物细胞和植物
公开(公告)号：US06215042B1
公开(公告)日：2001-04-10
申请号：US08104158
申请日：1993-08-13
申请人： Lothar Willmitzer , Uwe Sonnewald , Jens Kossmann , Bernd Müller-Röber , Richard G. F. Visser , Evert Jacobsen
发明人： Lothar Willmitzer , Uwe Sonnewald , Jens Kossmann , Bernd Müller-Röber , Richard G. F. Visser , Evert Jacobsen
IPC分类号： A01H500
CPC分类号： C12N9/107 , C12N15/8245
摘要： Plasmids are described having DNA sequences that after insertion into the genome of the plants cause changes in the carbohydrate concentration and the carbohydrate composition in regenerated plants. These changes can be obtained from a sequence of a branching enzyme that is located on these plasmids. This branching enzyme alters the amylose/amylopectin ratio in starch of the plants, especially in commercially used plants.
摘要翻译：描述的质粒具有插入植物基因组之后的DNA序列，引起再生植物中碳水化合物浓度和碳水化合物组成的变化。这些变化可以从位于这些质粒上的分支酶的序列获得。该分支酶改变植物淀粉中的直链淀粉/支链淀粉比例，特别是在商业上使用的植物中。

3. 发明授权

US5538879A Expression cassette and plasmids for a guard cell specific expression and their use for the introduction of transgenic plant cells and plants 失效
标题翻译：用于保卫细胞特异性表达的表达盒和质粒及其用于引入转基因植物细胞和植物的用途
公开(公告)号：US5538879A
公开(公告)日：1996-07-23
申请号：US140180
申请日：1994-01-24
申请人： Bernd Muller-Rober , Uwe Sonnewald , Lothar Willmitzer
发明人： Bernd Muller-Rober , Uwe Sonnewald , Lothar Willmitzer
IPC分类号： A01H1/00 , A01H5/00 , C12N5/10 , C12N9/12 , C12N15/29 , C12N15/82 , C07H21/04
CPC分类号： C12N9/1241 , C12N15/8245
摘要： A method of and plasmids for producing specific gene expression in the guard cells of plants. In particular, 5' transcriptional regulatory promoter regions are provided for guard cell specific expression. In addition, methods for preparing an expression cassette, plasmids, and the use of the cassettes and plasmids to produce transgenic plant cells and plants are described.
摘要翻译： PCT No.PCT / EP93 / 00489 371日期：1994年1月24日 102（e）日期1994年1月24日PCT 1993年3月3日PCT公布。公开号WO93 / 18169 日期1993年9月16日用于在植物的保卫细胞中产生特异性基因表达的方法和质粒。特别地，提供5'转录调节启动子区域用于保卫细胞特异性表达。此外，描述了制备表达盒，质粒以及使用盒和质粒产生转基因植物细胞和植物的方法。

4. 发明授权

US5723757A Plant promoters specific for sink organ expression of genes 失效
标题翻译：植物启动子特异于宿主器官表达的基因
公开(公告)号：US5723757A
公开(公告)日：1998-03-03
申请号：US448110
申请日：1995-05-23
申请人： Mario Rocha-Sosa , Uwe Sonnewald , Wolf-Bernd Frommer , Lothar Willmitzer , Marina Stratmann
发明人： Mario Rocha-Sosa , Uwe Sonnewald , Wolf-Bernd Frommer , Lothar Willmitzer , Marina Stratmann
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00 , C12N5/04
CPC分类号： C12N15/8235 , C12N15/8227
摘要： There are described 5' transcriptional regulatory regions of plant genes which ensure sink organ specific expression of cis-linked DNA sequences as well as recombinant double stranded DNA molecules comprising these 5' regulatory regions. Furthermore the present invention relates to a method for producing transgenic plants which show a sink tissue specific expression of a desired DNA sequence.
摘要翻译：描述了植物基因的5'转录调节区，其确保顺式连接的DNA序列的宿主器官特异性表达以及包含这5个调节区的重组双链DNA分子。此外，本发明涉及生产转化植物的方法，其显示所需DNA序列的宿组织特异性表达。

5. 发明授权

US5492820A Plasmids for the production of transgenic plants that are modified in habit and yield 失效
标题翻译：用于生产习惯和产量改良的转基因植物的质粒
公开(公告)号：US5492820A
公开(公告)日：1996-02-20
申请号：US147007
申请日：1993-11-02
申请人： Uwe Sonnewald , Lothar Willmitzer
发明人： Uwe Sonnewald , Lothar Willmitzer
IPC分类号： A01H5/00 , A01H1/00 , C12N5/10 , C12N9/14 , C12N15/09 , C12N15/55 , C12N15/82 , C12N15/00 , C12N15/31
CPC分类号： C12N15/8261 , C12N15/8245 , C12N9/14
摘要： Plasmids that comprise a DNA sequence coding for a protein that modifies the metabolism of soluble sugars, for example, an inorganic pyrophosphatase agent and plant cells comprising those plasmids, for the production of transgenic plants that are modified in habit and yield arc described, the plants being modified in habit by the transfer and the expression of genes that intervene in sugar metabolism or sugar distribution inside a plant.
摘要翻译：该植物包含编码可溶性糖代谢的蛋白质的DNA序列，例如无机焦磷酸酶试剂和包含这些质粒的植物细胞，用于生产以习惯和屈服弧度修饰的转基因植物，植物通过转移和干扰植物内糖代谢或糖分布的基因的表达习惯进行修饰。

6. 发明授权

US06762346B2 Nucleic acid molecules coding for debranching enzymes from maize 有权
标题翻译：编码玉米脱支酶的核酸分子
公开(公告)号：US06762346B2
公开(公告)日：2004-07-13
申请号：US09850991
申请日：2001-05-08
申请人： Jens Kossmann , Lothar Willmitzer , Michael Emmermann
发明人： Jens Kossmann , Lothar Willmitzer , Michael Emmermann
IPC分类号： C12N1582
CPC分类号： C12N9/2451 , C12N9/2457 , C12N15/8245 , C12Y302/01041
摘要： Nucleic acid molecules are described, which encode debranching enzymes from maize, as well as transgenic plant cells and plants in which an amylopectin with modified properties is synthesized due to the expression of a debranching enzyme from maize or due to the inhibition of such an endogeneous debranching enzyme activity.
摘要翻译：描述了核酸分子，其编码来自玉米的脱支酶，以及转基因植物细胞和植物，其中由于来自玉米的脱支酶的表达或由于抑制这种内源性脱支而合成具有改性特性的支链淀粉酶活性。

7. 发明授权

US5917127A Plasmids useful for and methods of preparing transgenic plants with modifications of habit and yield 失效
标题翻译：用于制备具有习惯和产量修饰的转基因植物的质粒和方法
公开(公告)号：US5917127A
公开(公告)日：1999-06-29
申请号：US450042
申请日：1995-05-25
申请人： Lothar Willmitzer , Uwe Sonnewald , Antje Von Schaeven
发明人： Lothar Willmitzer , Uwe Sonnewald , Antje Von Schaeven
IPC分类号： C07K14/81 , C12N9/26 , C12N15/29 , C12N15/82 , A01H4/00 , A01H5/00
CPC分类号： C12N9/2408 , C07K14/8114 , C12N15/8245
摘要： There is disclosed plasmids for the preparation of transgenic plants which are modified through the transfer and the expression of genes localized on the plasmids, that influence the sugar metabolism or the sugar partitioning within a plant. In addition, transgenic plants with modified sugar metabolism or sugar partitioning are described. The genes cause a modified distribution of assimilates in the transgenic plant which result in significant changes in plant habit, such as size, leaf shape, internode separation and root formation, as well as improvements in plant yield. Plasmids and methods are also described which enable foreign proteins to be directed into specific tissues or portions of transgenic plants, including vacuoles, leaves and sink organs.
摘要翻译：公开了用于制备转基因植物的质粒，其通过转移和定位在质粒上的基因的表达进行修饰，所述基因影响植物内的糖代谢或糖分配。此外，还描述了具有修饰的糖代谢或糖分配的转基因植物。这些基因导致转基因植物中同化物的修饰分布，导致植物习性的显着变化，如大小，叶形，节间分离和根形成，以及植物产量的提高。还描述了质粒和方法，其使外来蛋白质能够导入转基因植物的特定组织或部分，包括空泡，叶和宿器官。

8. 发明授权

US5436394A Plasmid for the preparation of transgenic plants with a change in habit and yield 失效
标题翻译：用于制备具有改变习惯和产量的转基因植物的质粒
公开(公告)号：US5436394A
公开(公告)日：1995-07-25
申请号：US156876
申请日：1993-11-23
申请人： Lothar Willmitzer , Uwe Sonnewald , Antje Von Schaeven
发明人： Lothar Willmitzer , Uwe Sonnewald , Antje Von Schaeven
IPC分类号： A01H5/00 , C07K14/81 , C12N5/10 , C12N9/26 , C12N15/09 , C12N15/56 , C12N15/82 , A01H1/00 , C12N5/14
CPC分类号： C12N9/2408 , C07K14/8114 , C12N15/8245
摘要： There is disclosed plasmids for the preparation of transgenic plants, as well as the plants, that are modified through the transfer and the expression of genes influencing the sugar metabolism or the sugar partitioning within a plant, which are localised on these plasmids.The transferred genes cause a modified distribution of assimilates in the transgenic plant which result in significant changes in habit, such as size, leaf shape, internode separation and root formation, as well as improvements in yield.Plasmids are also described which enable foreign proteins to be directed into the vacuoles of transgenic plants.
摘要翻译：公开了用于制备转基因植物以及植物的质粒，其通过转移和表达影响植物中糖代谢或基因分配的基因进行修饰，这些基因位于这些质粒上。转移的基因导致转基因植物中同化物的修饰分布，导致习性的显着变化，例如大小，叶形状，节间分离和根形成以及产量的提高。还描述了能够使外来蛋白质导入转基因植物的空泡中的质粒。

9. 发明授权

US5436393A Potato tuber specific transcriptional regulation 失效
标题翻译：马铃薯块茎特异性转录调控
公开(公告)号：US5436393A
公开(公告)日：1995-07-25
申请号：US995911
申请日：1992-12-22
申请人： Mario Rocha-Sosa , Uwe Sonnewald , Wolf-Bernd Frommer , Lothar Willmitzer , Marina Stratmann
发明人： Mario Rocha-Sosa , Uwe Sonnewald , Wolf-Bernd Frommer , Lothar Willmitzer , Marina Stratmann
IPC分类号： A01H1/00 , C07K14/415 , C12N1/21 , C12N5/10 , C12N9/14 , C12N15/09 , C12N15/29 , C12N15/82 , A01H1/04 , C07H21/04 , C12N5/00 , C12N15/00
CPC分类号： C12N15/8222 , C07K14/415 , C12N15/8227 , C12N15/8235 , C12N9/14
摘要： There is described a new DNA sequence of an expression cassette on which the potato tuber specific regulatory regions are localised as well as the transfer of this DNA sequence into the plant genome using agrobacteria as transfer micro-organisms. The DNA sequence contains a patatin gene with a patatin gene promoter. The transfer DNA sequence acts both for regulating endogenous as well as for preparation of heterologous products in crops.
摘要翻译：描述了一种表达盒的新DNA序列，其上马铃薯块茎特异性调节区域被定位，以及使用农杆菌作为转移微生物将该DNA序列转移到植物基因组中。 DNA序列含有具有patatin基因启动子的patatin基因。转移DNA序列既用于调节内源物质以及在作物中制备异源产物。

10. 发明申请

US20100145625A1 DETERMINATION AND PREDICTION OF THE EXPRESSION OF TRAITS OF PLANTS FROM THE METABOLITE PROFILE AS A BIOMARKER 审中-公开
公开(公告)号：US20100145625A1
公开(公告)日：2010-06-10
申请号：US12520845
申请日：2007-12-21
申请人： Thomas Altmann , Lothar Willmitzer , Joachim Selbig , Rhonda C. Meyer , Matthias Steinfath , Jan Lisec , Oliver Fiehn
发明人： Thomas Altmann , Lothar Willmitzer , Joachim Selbig , Rhonda C. Meyer , Matthias Steinfath , Jan Lisec , Oliver Fiehn
IPC分类号： G06F19/00 , G06F15/18 , G06N5/02 , C12Q1/68 , G06F17/10
CPC分类号： G01N33/5097 , G01N30/7206
摘要： The present invention relates to a method for determining the correlation between the metabolite profiles (MPs) and the expression or potential for expression of a trait of a group of plants, and further to a method for determining or predicting the expression of a trait of a plant by taking advantage of the determined MP of said plant and a determined correlation between the MPs and the expression or potential for the expression of said trait in a group of plants.Particularly, the present invention relates to a method for determining or predicting the biomass production/growth rate of a plant by taking advantage of the determined MP of said plant and a determined correlation between the MPs and the biomass production/growth rate or the potential for biomass production/growth rate of a group of plants.The present invention also relates to a method for breeding of a plant that takes advantage of a method of determining the expression or potential for expression of a trait of said plant according to any one of the methods as disclosed, and selecting said plant on the basis of the expression or potential for expression of said trait. Furthermore, the present invention relates to a method for identifying quantitative trait loci (QTLs) for a trait or for the potential for expression of a trait of a group of plants comprising the step of identifying QTLs for metabolite combinations showing correlation with the expression or potential for expression of said trait of said group of plants.The present invention also relates to and a method for identifying a candidate gene involved in the determination of expression or potential for expression of a trait of a plant comprising the step of isolating a gene corresponding to any one of the QTLs as identified by the corresponding method of the present invention.Additionally, the present invention relates to a method of screening for a plant that exhibits a desired expression or potential for expression of a trait as well as to a method of determining whether a treatment influences the expression or potential for expression of a trait of a plant.

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