专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20060003388A1 Sensitive intracellular calcium assay 有权
标题翻译：敏感的细胞内钙测定
公开(公告)号：US20060003388A1
公开(公告)日：2006-01-05
申请号：US11135779
申请日：2005-05-23
申请人： Linda Kauffman , Rajendra Singh , Edwin Ullman
发明人： Linda Kauffman , Rajendra Singh , Edwin Ullman
IPC分类号： G01N33/53
CPC分类号： G01N33/582
摘要： A sensitive intracellular calcium assay is disclosed comprising conveniently a reagent comprised of a dye precursor capable of entering cells and being hydrolyzed to a dye, whereby the dye complexes with calcium in said cells and provides a luminescent signal, an antibody specific for the dye and conjugated with a quencher, and a cellular anion exchange enzyme inhibitor. In performing the assay, the reagent is combined with cells expressing a receptor responsive to a ligand resulting in a change in cytosolic calcium. After incubation for the dye precursor to permeate the cells, the calcium may be determined by exciting the dye precursor and determining the peak fluorescence over a time course. The method can be used for measuring the effect of an agent on cytosolic calcium by binding to a cell surface membrane receptor.
摘要翻译：公开了敏感的细胞内钙测定法，其包括方便的是由能够进入细胞并被水解成染料的染料前体组成的试剂，由此所述染料与所述细胞中的钙络合并提供发光信号，对于染料和缀合的抗体与猝灭剂和细胞阴离子交换酶抑制剂。在进行测定时，试剂与表达对配体有影响的受体的细胞结合，导致细胞溶质钙的变化。在孵育染料前体渗透细胞后，可以通过激发染料前体并在时间过程中确定峰荧光来确定钙。该方法可用于通过结合细胞表面膜受体来测量药剂对细胞溶质钙的影响。

2. 发明申请

US20060199238A1 ADP detection using an enzyme-coupled reaction 有权
标题翻译：使用酶联反应的ADP检测
公开(公告)号：US20060199238A1
公开(公告)日：2006-09-07
申请号：US11357325
申请日：2006-02-17
申请人： Neil Charter , Richard Eglen , Rajendra Singh , Edwin Ullman
发明人： Neil Charter , Richard Eglen , Rajendra Singh , Edwin Ullman
IPC分类号： C12Q1/66 , C12Q1/26
CPC分类号： C12Q1/42
摘要： Methods and compositions are provided for determining ADP in the presence of ATP. These comprise including among the assay reagents at least one of the correcting components creatine phosphokinase and phosphocreatine, pyruvate kinase and phosphoenolpyruvate, peroxidase and a non-interfering peroxidase substrate, and catalase. One aspect of the method employs formation of hydrogen peroxide from the ADP by pyruvate kinase, phosphoenolpyruvate and pyruvate oxidase. The hydrogen peroxide is then determined. A combined reagent having all of the reagents may optionally include a peroxidase when the hydrogen peroxide is to be enzymatically determined. A peroxidase substrate is added to the sample in conjunction with the peroxidase substrate reagent, the mixture incubated and depending on whether the peroxidase substrate is a fluorescer or chemiluminescer, the mixture may be illuminated with excitation light and the emitted light determined as a measure of the ADP in the sample.
摘要翻译：提供了在ATP存在下测定ADP的方法和组合物。这些包括测定试剂中的至少一种校正组分肌酸磷酸激酶和磷酸肌酸，丙酮酸激酶和磷酸烯醇丙酮酸，过氧化物酶和非干扰性过氧化物酶底物，以及过氧化氢酶。该方法的一个方面是通过丙酮酸激酶，磷酸烯醇丙酮酸和丙酮酸氧化酶从ADP形成过氧化氢。然后测定过氧化氢。当要酶法测定过氧化氢时，具有所有试剂的组合试剂可以任选地包括过氧化物酶。将过氧化物酶底物与过氧化物酶底物试剂一起加入到样品中，混合物孵育，并且取决于过氧化物酶底物是荧光剂还是化学发光剂，可以用激发光照射混合物，并且将测定的发射光作为 ADP在样品中。

3. 发明授权

US06379899B1 Isothermal exponential RNA amplification in complex mixtures 失效
标题翻译：复杂混合物中的等温指数RNA扩增
公开(公告)号：US06379899B1
公开(公告)日：2002-04-30
申请号：US09805674
申请日：2001-03-13
申请人： Edwin Ullman , Ming Wu
发明人： Edwin Ullman , Ming Wu
IPC分类号： C12Q168
CPC分类号： C12Q1/6865 , C12Q2525/143 , C12Q2525/301
摘要： Methods and compositions are provided for performing isothermal amplification of a nucleic acid target employing probes characterized by having a masked RNA polymer promoter unable to bind to a complementary initiator oligonucleotide and RNA polymerase and initiate transcription, a dsDNA sequence which when invaded by the target nucleic acid exposes the masked promoter to initiate transcription, and a template sequence, a portion of which is normally included in the dsDNA region, which when copied produces a product that can reinitiate the process of invading the dsDNA region and initiating transcription of another copy.
摘要翻译：提供了方法和组合物，用于使用探针的等温扩增，所述探针的特征在于具有不能结合互补引发剂寡核苷酸和RNA聚合酶并启动转录的掩蔽的RNA聚合物启动子，当被靶核酸侵入时，暴露掩蔽的启动子以启动转录，并且模板序列（其一部分通常包含在dsDNA区域中），其在复制时产生可以重新引入入侵dsDNA区域并启动另一拷贝的转录的产物。

4. 发明申请

US20050214875A1 Metal chelate containing compositions for use in chemiluminescent assays 有权
公开(公告)号：US20050214875A1
公开(公告)日：2005-09-29
申请号：US11132399
申请日：2005-05-19
申请人： Sharat Singh , Edwin Ullman
发明人： Sharat Singh , Edwin Ullman
IPC分类号： G01N33/532 , C07D265/30 , C07D279/12 , C07D327/06 , C07D413/04 , C07F5/00 , C07F15/00 , C12Q1/26 , G01N21/78 , G01N33/53 , G01N33/533 , G01N33/542 , G01N33/543 , G01N33/58
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , C07D413/04 , G01N33/533 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/968 , Y10S436/80 , Y10S436/805 , Y10S436/808 , Y10T436/13
摘要： Compositions are disclosed comprising (a) a metal chelate wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium in at least a hexacoordinated state and (b) a compound having a double bond substituted with two aryl groups, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen wherein one of the aryl groups is electron donating with respect to the other. Such composition is preferably incorporated in a latex particulate material. Methods and kits are also disclosed for determining an analyte in a medium suspected of containing the analyte. The methods and kits employ as one component a composition as described above.

5. 发明申请

US20050191676A1 Detection of nucleic acids by target-catalyzed product formation 审中-公开
标题翻译：通过靶催化产物形成检测核酸
公开(公告)号：US20050191676A1
公开(公告)日：2005-09-01
申请号：US11054255
申请日：2005-02-09
申请人： Linda Western , Samuel Rose , Edwin Ullman
发明人： Linda Western , Samuel Rose , Edwin Ullman
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/689 , C12Q1/682 , C12Q1/6823 , C12Q2521/319 , C12Q2537/149 , C12Q2521/301 , C12Q2563/101
摘要： A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide, for example, a polynucleotide analyte, in the presence of a 5′-nuclease under isothermal conditions. The polynucleotide analyte serves as a recognition element to enable a 5′-nuclease to cleave the oligonucleotide to provide (i) a first fragment-that is substantially non-hybridizable to the polynucleotide analyte and (ii) a second fragment that lies 3′ of the first fragment (in the intact oligonucleotide) and is substantially hybridizable to the polynucleotide analyte. At least a 100-fold molar excess of the first fragment and/or the second fragment are obtained relative to the molar amount of the polynucleotide analyte. The presence of the first fragment and/or the second fragment is detected, the presence thereof indicating the presence of the polynucleotide analyte. The method has particular application to the detection of a polynucleotide analyte such as DNA. Kits for conducting methods in accordance with the present invention are also disclosed.
摘要翻译：公开了一种修饰寡核苷酸的方法，该方法可用于检测多核苷酸分析物。在等温条件下，在5'-核酸酶的存在下，寡核苷酸与多核苷酸例如多核苷酸分析物可逆地杂交。多核苷酸分析物用作识别元件，以使5'-核酸酶切割寡核苷酸以提供（i）与多核苷酸分析物基本上不可杂交的第一片段和（ii）位于3'末端的第3个片段第一片段（在完整寡核苷酸中）并且与多核苷酸分析物基本上可杂交。相对于多核苷酸分析物的摩尔量，获得至少100倍摩尔过量的第一片段和/或第二片段。检测到第一片段和/或第二片段的存在，其存在表明多核苷酸分析物的存在。该方法特别适用于多核苷酸分析物如DNA的检测。还公开了用于根据本发明的方法的套件。

6. 发明申请

US20060121506A1 Particles for diagnostic and therapeutic use 有权
标题翻译：用于诊断和治疗用途的颗粒
公开(公告)号：US20060121506A1
公开(公告)日：2006-06-08
申请号：US11273275
申请日：2005-11-14
申请人： Sharat Singh , John Pease , Jacqueline Sadakian , Daniel Wagner , Edwin Ullman
发明人： Sharat Singh , John Pease , Jacqueline Sadakian , Daniel Wagner , Edwin Ullman
IPC分类号： C12Q1/68 , C07H21/04 , C12M1/34
CPC分类号： G01N33/587 , C12Q1/6816 , Y10T436/10 , C12Q2563/155 , C12Q2523/319
摘要： Methods, compositions and kits are disclosed. The compositions are light emitting and comprise a polymeric matrix having dissolved therein a photoactive compound. The composition has the characteristic that, after activation of the photoactive compound, the rate of decrease in the intensity of light emission at any time during a 20-fold decrease in the intensity is proportional to the intensity of the light emission. In one embodiment the polymeric matrix is comprised of particles of about 20 nm to about 100 μm in diameter to which is bound a specific binding pair member. The particles generally comprise a polymeric matrix having dissolved therein about 1 to about 20% by weight of a dopant. The compositions may be used in methods for determining an analyte. A combination is provided comprising (1) a medium suspected of containing the analyte, (2) and the aforementioned composition. The photoactive substance is activated and the effect of the activating on the optical properties of the combination is detected. The presence and amount of the effect is related to the presence and amount of the analyte in the medium. Also disclosed are kits for use in an assay.
摘要翻译：公开了方法，组合物和试剂盒。组合物是发光的并且包含其中溶解有光敏化合物的聚合物基质。该组合物具有以下特征：在光活性化合物活化后，强度降低20倍的任何时间的发光强度的降低率与发光强度成比例。在一个实施方案中，聚合物基质包括直径约20nm至约100μm的颗粒，其与特定结合对成员结合。颗粒通常包含其中溶解约1至约20重量％的掺杂剂的聚合物基质。该组合物可用于测定分析物的方法中。提供的组合包括（1）怀疑含有分析物的介质，（2）和上述组合物。光活性物质被激活，并且检测到激活对组合的光学性质的影响。影响的存在和数量与介质中分析物的存在和数量有关。还公开了用于测定的试剂盒。

7. 发明申请

US20060099664A1 Simultaneous screening of multiple analytes 有权
公开(公告)号：US20060099664A1
公开(公告)日：2006-05-11
申请号：US11268323
申请日：2005-11-07
申请人： Edwin Ullman , Marcel Pirio , Mary Ericson , Daniel Wagner , Dariush Davalian
发明人： Edwin Ullman , Marcel Pirio , Mary Ericson , Daniel Wagner , Dariush Davalian
IPC分类号： G01N33/537
CPC分类号： G01N33/946 , G01N33/54386 , Y10S435/975 , Y10S436/805 , Y10S436/811 , Y10S436/815 , Y10S436/816 , Y10S436/817 , Y10S436/901
摘要： Methods, compositions and kits are disclosed. The methods are directed to determining the presence of one or more analytes in a sample suspected of containing any one of a plurality of the analytes. A combination is provided comprising in a medium (i) the sample, (ii) a binding partner for each of the analytes, (iii) for each of the analytes, a first reagent comprising a member of a signal producing system, a ligand and an analyte analog, and (iv) a second reagent comprising a binding partner for the ligand. The binding of the binding partner for the ligand is affected by the presence of an analyte and alters the amount of signal produced by the member of the signal producing system. The signal thus is modulated if one or more of the analytes are present in the sample. The amount of the signal is determined and is related to the presence of one or more of the analytes in the sample. The method may be homogeneous or heterogeneous.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式