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    • 1. 发明申请
    • GRG23 EPSP SYNTHASES: COMPOSITIONS AND METHODS OF USE
    • GRG23 EPSP合成:组合物和使用方法
    • US20110236950A1
    • 2011-09-29
    • US12856082
    • 2010-08-13
    • Laura C. SchoutenCheryl L. PetersBrian Vande BergVolker Heinrichs
    • Laura C. SchoutenCheryl L. PetersBrian Vande BergVolker Heinrichs
    • C12N9/00
    • C12N15/8275C12N9/1085C12N9/1092
    • Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.
    • 提供了赋予除草剂抗性或对细菌,植物,植物细胞,组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸,包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体(包括微生物和植物)中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了分离的多核苷酸,其含有编码SEQ ID NO:9,11,13,15,17,19,21,23,25,27,29,31,33, 或35,或SEQ ID NO:6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。
    • 4. 发明申请
    • DIRECTED EVOLUTION OF GRG31 AND GRG36 EPSP SYNTHASE ENZYMES
    • GRG31和GRG36 EPSP合成酶的指导进化
    • US20090205076A1
    • 2009-08-13
    • US12362774
    • 2009-01-30
    • Volker HeinrichsLaura Cooper SchoutenBrian Vande Berg
    • Volker HeinrichsLaura Cooper SchoutenBrian Vande Berg
    • A01H1/00C07H21/00C12N15/63C12N1/21C12N5/04A01H5/00C12N9/00C12P21/00
    • C12N15/8275C12N9/1092
    • Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
    • 提供了赋予除草剂抗性或对细菌,植物,植物细胞,组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸,包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体(包括微生物和植物)中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸,特别是SEQ ID NO:2和4的多肽变体。此外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了含有编码SEQ ID NO:7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO:29或30所示的核苷酸序列的分离的多核苷酸。
    • 5. 发明授权
    • Directed evolution of GRG31 EPSP synthase enzyme
    • GRG31 EPSP合成酶的定向进化
    • US08247535B2
    • 2012-08-21
    • US13101351
    • 2011-05-05
    • Volker HeinrichsLaura Cooper SchoutenBrian Vande Berg
    • Volker HeinrichsLaura Cooper SchoutenBrian Vande Berg
    • C12N15/88A61K38/43A61K380/00
    • C12N15/8275C12N9/1092
    • Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
    • 提供了赋予除草剂抗性或对细菌,植物,植物细胞,组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸,包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体(包括微生物和植物)中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸,特别是SEQ ID NO:2和4的多肽变体。此外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了含有编码SEQ ID NO:7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO:29或30所示的核苷酸序列的分离的多核苷酸。
    • 6. 发明授权
    • Directed evolution of GRG31 EPSP synthase enzyme
    • GRG31 EPSP合成酶的定向进化
    • US07960616B2
    • 2011-06-14
    • US12362774
    • 2009-01-30
    • Volker HeinrichsLaura Cooper SchoutenBrian Vande Berg
    • Volker HeinrichsLaura Cooper SchoutenBrian Vande Berg
    • C12N15/82C12N15/52
    • C12N15/8275C12N9/1092
    • Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
    • 提供了赋予除草剂抗性或对细菌,植物,植物细胞,组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸,包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体(包括微生物和植物)中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸,特别是SEQ ID NO:2和4的多肽变体。此外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了含有编码SEQ ID NO:7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO:29或30所示的核苷酸序列的分离的多核苷酸。
    • 7. 发明授权
    • GRG23 EPSP synthases: compositions and methods of use
    • GRG23 EPSP合成酶:组合物和使用方法
    • US08283523B2
    • 2012-10-09
    • US12856072
    • 2010-08-13
    • Laura Cooper SchoutenBrian Vande BergVolker Heinrichs
    • Laura Cooper SchoutenBrian Vande BergVolker Heinrichs
    • C12N15/82C12N15/63C12N15/52
    • C12N15/8275C12N9/1085C12N9/1092
    • Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
    • 提供了赋予除草剂抗性或对细菌,植物,植物细胞,组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸,包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体(包括微生物和植物)中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。 另外,包括对应于多核苷酸的氨基酸序列。
    • 8. 发明授权
    • GRG23 EPSP synthases: compositions and methods of use
    • GRG23 EPSP合成酶:组合物和使用方法
    • US08252981B2
    • 2012-08-28
    • US12856058
    • 2010-08-13
    • Laura Cooper SchoutenBrian Vande BergVolker Heinrichs
    • Laura Cooper SchoutenBrian Vande BergVolker Heinrichs
    • C12N1/21C12N15/52C12N15/82
    • C12N15/8275C12N9/1085C12N9/1092
    • Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 33, or the nucleotide sequence set forth in SEQ ID NO: 32.
    • 提供了赋予除草剂抗性或对细菌,植物,植物细胞,组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸,包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体(包括微生物和植物)中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了含有编码SEQ ID NO:33所示的氨基酸序列的核苷酸序列或SEQ ID NO:32所示的核苷酸序列的分离的多核苷酸。
    • 9. 发明申请
    • DIRECTED EVOLUTION OF GRG31 EPSP SYNTHASE ENZYME
    • GRG31 EPSP合成酶的指导进化
    • US20110223648A1
    • 2011-09-15
    • US13101351
    • 2011-05-05
    • Volker HeinrichsLaura Cooper SchoutenBrian Vande Berg
    • Volker HeinrichsLaura Cooper SchoutenBrian Vande Berg
    • C12N9/10
    • C12N15/8275C12N9/1092
    • Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
    • 提供了赋予除草剂抗性或对细菌,植物,植物细胞,组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸,包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体(包括微生物和植物)中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸,特别是SEQ ID NO:2和4的多肽变体。此外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了含有编码SEQ ID NO:7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO:29或30所示的核苷酸序列的分离的多核苷酸。
    • 10. 发明申请
    • AXMI205 VARIANT PROTEINS AND METHODS OF USE
    • AXMI205变种蛋白及其使用方法
    • US20140298538A1
    • 2014-10-02
    • US14233426
    • 2012-07-27
    • Volker HeinrichsJayme Williams
    • Volker HeinrichsJayme Williams
    • C12N15/82C07K16/12C07K14/22A01N37/44
    • C12N15/8279A01N37/46A01N63/02A01N65/00C07K14/325C12N15/8286Y02A40/162
    • Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7, 8, 9, 10, 11, or 12, the nucleotide sequence set forth in SEQ ID NO:4, 5, or 6, as well as variants and fragments thereof.
    • 提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的杀虫核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了包含编码SEQ ID NO:7,8,9,10,11或12所示氨基酸序列的核酸分子的核酸分子,SEQ ID NO:4所示的核苷酸序列, 5或6,以及其变体和片段。