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1. 发明授权

US08247535B2 Directed evolution of GRG31 EPSP synthase enzyme 有权
标题翻译： GRG31 EPSP合成酶的定向进化
公开(公告)号：US08247535B2
公开(公告)日：2012-08-21
申请号：US13101351
申请日：2011-05-05
申请人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
发明人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： C12N15/88 , A61K38/43 , A61K380/00
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。

2. 发明授权

US07960616B2 Directed evolution of GRG31 EPSP synthase enzyme 有权
标题翻译： GRG31 EPSP合成酶的定向进化
公开(公告)号：US07960616B2
公开(公告)日：2011-06-14
申请号：US12362774
申请日：2009-01-30
申请人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
发明人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： C12N15/82 , C12N15/52
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。

3. 发明授权

US08450267B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08450267B2
公开(公告)日：2013-05-28
申请号：US12856082
申请日：2010-08-13
申请人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
发明人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
IPC分类号： C07K14/00 , C12N15/11
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：9,11,13,15,17,19,21,23,25,27,29,31,33，或35，或SEQ ID NO：6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。

4. 发明授权

US08283523B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08283523B2
公开(公告)日：2012-10-09
申请号：US12856072
申请日：2010-08-13
申请人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
发明人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
IPC分类号： C12N15/82 , C12N15/63 , C12N15/52
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。

5. 发明授权

US08252981B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08252981B2
公开(公告)日：2012-08-28
申请号：US12856058
申请日：2010-08-13
申请人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
发明人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
IPC分类号： C12N1/21 , C12N15/52 , C12N15/82
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 33, or the nucleotide sequence set forth in SEQ ID NO: 32.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：33所示的氨基酸序列的核苷酸序列或SEQ ID NO：32所示的核苷酸序列的分离的多核苷酸。

6. 发明申请

US20110223648A1 DIRECTED EVOLUTION OF GRG31 EPSP SYNTHASE ENZYME 有权
标题翻译： GRG31 EPSP合成酶的指导进化
公开(公告)号：US20110223648A1
公开(公告)日：2011-09-15
申请号：US13101351
申请日：2011-05-05
申请人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
发明人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： C12N9/10
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。

7. 发明申请

US20110136200A1 Bacterial Glutamine Synthetases and Methods of Use 有权
标题翻译：细菌谷氨酰胺合成酶和使用方法
公开(公告)号：US20110136200A1
公开(公告)日：2011-06-09
申请号：US13027666
申请日：2011-02-15
申请人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
发明人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
IPC分类号： C12N9/00
CPC分类号： C12N15/8277 , C12N9/93
摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO:1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.
摘要翻译：提供了赋予除草剂抵抗和改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。另外，包括对应于多核苷酸的多肽。特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。

8. 发明授权

US08101389B2 Bacterial glutamine synthetases and methods of use 有权
标题翻译：细菌性谷氨酰胺合成酶及其使用方法
公开(公告)号：US08101389B2
公开(公告)日：2012-01-24
申请号：US13027666
申请日：2011-02-15
申请人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
发明人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
IPC分类号： C12N9/00
CPC分类号： C12N15/8277 , C12N9/93
摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO:1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.
摘要翻译：提供了赋予除草剂抵抗和改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。另外，包括对应于多核苷酸的多肽。特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。

9. 发明授权

US08309332B2 GRG23 and GRG51 polypeptides having herbicide resistance activity 有权
标题翻译：具有除草剂抗性活性的GRG23和GRG51多肽
公开(公告)号：US08309332B2
公开(公告)日：2012-11-13
申请号：US12468205
申请日：2009-05-19
申请人： Cheryl L. Peters , Jill Burdette Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Burdette Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
IPC分类号： C12N9/00
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

10. 发明授权

US08252980B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08252980B2
公开(公告)日：2012-08-28
申请号：US12856021
申请日：2010-08-13
申请人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
发明人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
IPC分类号： C12N1/21 , C12N15/52 , C12N15/82
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 31, or the nucleotide sequence set forth in SEQ ID NO: 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：31所示的氨基酸序列的核苷酸序列或SEQ ID NO：30所示的核苷酸序列的分离的多核苷酸。

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