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1. 发明授权

US07935518B2 Smart hydrogel particles for biomarker harvesting 有权
标题翻译：用于生物标志物收获的智能水凝胶颗粒
公开(公告)号：US07935518B2
公开(公告)日：2011-05-03
申请号：US12033701
申请日：2008-02-19
申请人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , David Geho
发明人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , David Geho
IPC分类号： G01N33/545
CPC分类号： B01J20/26 , B01D15/34 , B01J20/265 , B01J20/28078 , B01J20/28095 , B01J2220/54 , C12Q1/6816 , G01N33/54313 , G01N33/54353 , G01N33/5436 , Y10S435/81 , Y10S436/808 , Y10T436/255 , C12Q2565/518
摘要： Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles may be changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.
摘要翻译：描述从溶液中收集分析物的捕获颗粒和使用它们的方法。捕获颗粒由具有允许分析物进入捕获颗粒的孔径的聚合物基质构成。当对颗粒施加刺激时，捕获颗粒的孔径可以变化，允许改变颗粒的孔径，使得目标分析物保留在颗粒内。捕获颗粒的聚合物基质可以由具有结构单体和亲和单体的共聚物制成，亲和单体具有将分析物吸引到捕获颗粒的性质。捕获颗粒可用于分离和鉴定混合物中存在的分析物。它们也可用于保护在收获时通常会降解的分析物，并将低分析物浓缩在液体中的丰度低。

2. 发明申请

US20090148961A1 SMART HYDROGEL PARTICLES FOR BIOMARKER HARVESTING 有权
标题翻译：用于生物标记收集的SMART HYDROGEL颗粒
公开(公告)号：US20090148961A1
公开(公告)日：2009-06-11
申请号：US12033701
申请日：2008-02-19
申请人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , David Geho
发明人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , David Geho
IPC分类号： G01N33/543 , B01J19/00 , G01N27/00 , G01N21/00 , G01N21/64 , C12M1/00 , C08J9/16
CPC分类号： B01J20/26 , B01D15/34 , B01J20/265 , B01J20/28078 , B01J20/28095 , B01J2220/54 , C12Q1/6816 , G01N33/54313 , G01N33/54353 , G01N33/5436 , Y10S435/81 , Y10S436/808 , Y10T436/255 , C12Q2565/518
摘要： Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles may be changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.
摘要翻译：描述从溶液中收集分析物的捕获颗粒和使用它们的方法。捕获颗粒由具有允许分析物进入捕获颗粒的孔径的聚合物基质构成。当对颗粒施加刺激时，捕获颗粒的孔径可以变化，允许改变颗粒的孔径，使得目标分析物保留在颗粒内。捕获颗粒的聚合物基质可以由具有结构单体和亲和单体的共聚物制成，亲和单体具有将分析物吸引到捕获颗粒的性质。捕获颗粒可用于分离和鉴定混合物中存在的分析物。它们也可用于保护在收获时通常会降解的分析物，并将低分析物浓缩在液体中的丰度低。

3. 发明申请

US20080069774A1 PROTEOMIC ANTISENSE MOLECULAR SHIELD AND TARGETING 审中-公开
标题翻译： PROTEOMIC ANTISENSE MILOSILS SHIELD AND TARGETING
公开(公告)号：US20080069774A1
公开(公告)日：2008-03-20
申请号：US11733070
申请日：2007-04-09
申请人： Lance Liotta , David Geho , Emanuel Petricoin
发明人： Lance Liotta , David Geho , Emanuel Petricoin
IPC分类号： A61F13/00 , A61F2/06 , A61K31/20 , A61K31/70 , A61K38/00 , A61K49/00 , A61P9/00 , C07C53/00 , C07H1/00 , C07H21/04 , C07K14/00 , C07K16/18
CPC分类号： A61K38/39 , A61F2/064 , A61K31/20 , A61K31/70 , A61K47/60 , A61K47/6435 , A61K47/6931 , A61K49/0002
摘要： The present invention provides compositions and methods for shielding and directing agents to biological targets in cellular systems for therapeutic, prophylactic, and diagnostic uses. Vascular devices are also provided which have coated surfaces that contain proteomic antisense, as well as therapeutic and other biological agents attached thereto.
摘要翻译：本发明提供了用于屏蔽和引导剂到用于治疗，预防和诊断用途的细胞系统中的生物靶标的组合物和方法。还提供了具有包含蛋白质组学反义物的包被表面以及与其连接的治疗剂和其它生物学试剂的血管装置。

4. 发明授权

US09005996B2 Method of isolating analytes from a sample 有权
标题翻译：从样品中分离分析物的方法
公开(公告)号：US09005996B2
公开(公告)日：2015-04-14
申请号：US11527727
申请日：2006-09-27
申请人： Lance Liotta , Emanuel Petricoin , David Geho
发明人： Lance Liotta , Emanuel Petricoin , David Geho
IPC分类号： G01N33/545 , G01N33/543
CPC分类号： G01N33/54346 , Y10T428/2982 , Y10T428/2991 , Y10T428/2998
摘要： The current invention is a capture-particle comprising: a) a molecular sieve portion; and b) an analyte binding portion; wherein the molecular sieve portion, analyte binding portion or both further comprise a cross-linked region having modified porosity. Capture particles wherein the molecular sieve portion, analyte binding portion or both comprise pore dimensions sufficient to exclude molecules larger than about 60 kDa. These particles are useful in purification and diagnostic methods. Kits comprising the capture particles are also described.
摘要翻译：本发明是捕获颗粒，其包含：a）分子筛部分; 和b）分析物结合部分; 其中所述分子筛部分，分析物结合部分或两者还包含具有改变的孔隙率的交联区域。捕获颗粒，其中分子筛部分，分析物结合部分或两者都包含足以排除大于约60kDa的分子的孔尺寸。这些颗粒可用于纯化和诊断方法。还描述了包含捕获颗粒的试剂盒。

5. 发明申请

US20130243700A1 Proteomic Antisense Molecular Shield and Targeting 审中-公开
标题翻译：蛋白质反义分子屏蔽和靶向
公开(公告)号：US20130243700A1
公开(公告)日：2013-09-19
申请号：US13036902
申请日：2011-02-28
申请人： Lance Liotta , David Geho , Emanuel Petricoin
发明人： Lance Liotta , David Geho , Emanuel Petricoin
IPC分类号： A61K38/39 , A61K47/48 , A61F2/06 , A61K49/00
CPC分类号： A61K38/39 , A61F2/064 , A61K31/20 , A61K31/70 , A61K47/60 , A61K47/6435 , A61K47/6931 , A61K49/0002
摘要： The present invention provides compositions and methods for shielding and directing agents to biological targets in cellular systems for therapeutic, prophylactic, and diagnostic uses. Vascular devices are also provided which have coated surfaces that contain proteomic antisense, as well as therapeutic and other biological agents attached thereto.
摘要翻译：本发明提供了用于屏蔽和引导剂到用于治疗，预防和诊断用途的细胞系统中的生物靶标的组合物和方法。还提供了具有包含蛋白质组学反义物的包被表面以及与其连接的治疗剂和其它生物学试剂的血管装置。

6. 发明申请

US20090305432A1 Polypeptide Molecular Switch 审中-公开
标题翻译：多肽分子开关
公开(公告)号：US20090305432A1
公开(公告)日：2009-12-10
申请号：US12083678
申请日：2006-10-16
申请人： Lance Liotta , Emanuel Petricoin , David Geho
发明人： Lance Liotta , Emanuel Petricoin , David Geho
IPC分类号： G01N33/53 , C07K7/00 , C07K7/06 , C12N9/12 , G11C7/10 , H01L29/772 , G01N27/00
CPC分类号： G01N33/5438 , B82Y10/00 , B82Y15/00 , B82Y30/00 , C12Q1/485 , G01N33/54373 , G01N2333/91215
摘要： A polypeptide can conduct electricity in a closed circuit. Conformational changes in the polypeptide due to posttranslational modifications or ligand binding can effect the conductive properties of the polypeptide which can be measured. In such a closed circuit, a polypeptide having at least one residue capable of reversible modification can be used as a molecular switch. Circuits comprising such molecular switches can be used, for example, in methods for assessing the modification state of a polypeptide, determining the activity of an enzyme of interest, identifying compounds that affect the activity of an enzyme of interest, storing data, detecting the presence of a compound and identifying inhibitors of protein-protein interactions.
摘要翻译：多肽可以在闭路中导电。由于翻译后修饰或配体结合导致的多肽的构象变化可影响可测量的多肽的导电性质。在这样的闭路中，可以使用具有至少一个能够进行可逆修饰的残基的多肽作为分子开关。包括这种分子开关的电路可以用于例如用于评估多肽的修饰状态，确定感兴趣的酶的活性，鉴定影响感兴趣的酶的活性的化合物的方法，存储数据，检测存在的化合物和鉴定蛋白质 - 蛋白质相互作用的抑制剂。

7. 发明申请

US20100240543A1 Method of isolating analytes from a sample 有权
标题翻译：从样品中分离分析物的方法
公开(公告)号：US20100240543A1
公开(公告)日：2010-09-23
申请号：US11527727
申请日：2006-09-27
申请人： Lance Liotta , Emanuel Petricoin , David Geho
发明人： Lance Liotta , Emanuel Petricoin , David Geho
IPC分类号： G01N33/53 , C08F20/56 , C07K1/22 , C40B30/04 , C12Q1/68
CPC分类号： G01N33/54346 , Y10T428/2982 , Y10T428/2991 , Y10T428/2998
摘要： The current invention is a capture-particle comprising: a) a molecular sieve portion; and b) an analyte binding portion; wherein the molecular sieve portion, analyte binding portion or both further comprise a cross-linked region having modified porosity. Capture particles wherein the molecular sieve portion, analyte binding portion or both comprise pore dimensions sufficient to exclude molecules larger than about 60 kDa. These particles are useful in purification and diagnostic methods. Kits comprising the capture particles are also described.
摘要翻译：本发明是捕获颗粒，其包含：a）分子筛部分; 和b）分析物结合部分; 其中所述分子筛部分，分析物结合部分或两者还包含具有改变的孔隙率的交联区域。捕获颗粒，其中分子筛部分，分析物结合部分或两者都包含足以排除大于约60kDa的分子的孔尺寸。这些颗粒可用于纯化和诊断方法。还描述了包含捕获颗粒的试剂盒。

8. 发明授权

US08753897B2 Nanoporous substrates for the analytical methods 有权
标题翻译：纳米多孔基材用于分析方法
公开(公告)号：US08753897B2
公开(公告)日：2014-06-17
申请号：US11641970
申请日：2006-12-20
申请人： Mauro Ferrari , Mark Ming-Cheng Cheng , Giovanni Cuda , Marco Gaspari , David Geho , Lance Liotta , Emmanuel Petricoin , Fredika Robertson , Rosa Terracciano
发明人： Mauro Ferrari , Mark Ming-Cheng Cheng , Giovanni Cuda , Marco Gaspari , David Geho , Lance Liotta , Emmanuel Petricoin , Fredika Robertson , Rosa Terracciano
IPC分类号： G01N1/18
CPC分类号： G01N1/405 , B01D15/00 , B01D15/08 , B01J20/103 , B01J20/2808 , B01J2220/54 , B01L3/5023 , B82Y5/00 , C07K1/30 , C07K1/34 , G01N33/6803 , Y10T436/2525 , Y10T436/255
摘要： Nanoporous materials can be used to enrich samples for subsequent analysis of substances contained in the sample. The method is shown to enrich the yield of species in the low molecular weight proteome, allowing detection of small peptides in the low nanomolar range.
摘要翻译：可以使用纳米多孔材料来富集样品，以便随后分析样品中所含的物质。该方法显示为丰富低分子量蛋白质组中物种的产量，允许检测低纳摩尔范围内的小肽。

9. 发明申请

US20080277578A1 Nanoporous substrates for the analytical methods 有权
标题翻译：纳米多孔基材用于分析方法
公开(公告)号：US20080277578A1
公开(公告)日：2008-11-13
申请号：US11641970
申请日：2006-12-20
申请人： Mauro Ferrari , Mark Ming-Cheng Cheng , Giovanni Cuda , Marco Gaspari , David Geho , Lance Liotta , Emmanuel Petricoin , Fredika Robertson , Rosa Terracciano
发明人： Mauro Ferrari , Mark Ming-Cheng Cheng , Giovanni Cuda , Marco Gaspari , David Geho , Lance Liotta , Emmanuel Petricoin , Fredika Robertson , Rosa Terracciano
IPC分类号： H01J49/04
CPC分类号： G01N1/405 , B01D15/00 , B01D15/08 , B01J20/103 , B01J20/2808 , B01J2220/54 , B01L3/5023 , B82Y5/00 , C07K1/30 , C07K1/34 , G01N33/6803 , Y10T436/2525 , Y10T436/255
摘要： Nanoporous materials can be used to enrich samples for subsequent analysis of substances contained in the sample. The method is shown to enrich the yield of species in the low molecular weight proteome, allowing detection of small peptides in the low nanomolar range.
摘要翻译：可以使用纳米多孔材料来富集样品，以便随后分析样品中所含的物质。该方法显示为丰富低分子量蛋白质组中物种的产量，允许检测低纳摩尔范围内的小肽。

10. 发明申请

US20130345075A1 Tissue Preservation and Fixation Method 有权
标题翻译：组织保存和固定方法
公开(公告)号：US20130345075A1
公开(公告)日：2013-12-26
申请号：US13836995
申请日：2013-03-15
申请人： Virginia A. Espina , Lance A. Liotta , David Geho
发明人： Virginia A. Espina , Lance A. Liotta , David Geho
IPC分类号： G01N1/30
CPC分类号： G01N1/30 , A01N1/00
摘要： This invention relates, e.g., to a composition that, at room temperature, when contacted with a sample comprising phosphoproteins, can fix and stabilize cellular phosphoproteins, preserve cellular morphology, and allow the sample to be frozen to generate a cryostat frozen section suitable for molecular analysis. The composition comprises (1) a fixative that stabilizes the proteins in the sample and that has a sufficient water content for a stabilizer and/or a permeability enhancing agent to be soluble therein; (2) a stabilizer, comprising (a) a kinase inhibitor and (b) a phosphatase inhibitor and, optionally, (c) a protease (e.g., proteinase) inhibitor; (3) a permeability enhancing agent; and (4) lactic acid. Methods and kits are described for preserving phosphoproteins, using such a composition. Also described are endogenous surrogate markers for monitoring protein degradation, including the loss of posttranslational modifications (such as phosphorylation), e.g. following removal of a cell or tissue from a subject; and exogenous molecular sentinels (e.g. phosphoproteins attached to magnetic nanoparticles) that allow one to evaluate the processing history of a cellular or tissue population sample.
摘要翻译：本发明涉及例如在室温下与包含磷蛋白的样品接触时可以固定和稳定细胞磷酸蛋白的组合物，保留细胞形态，并允许样品冷冻以产生适于分子的低温恒温器冷冻切片分析。组合物包含（1）稳定样品中蛋白质并且具有足够的水含量的固定剂，用于稳定剂和/或渗透性增强剂可溶于其中; （2）稳定剂，其包含（a）激酶抑制剂和（b）磷酸酶抑制剂和任选的（c）蛋白酶（例如蛋白酶）抑制剂; （3）渗透增强剂; 和（4）乳酸。描述了使用这种组合物来保存磷蛋白的方法和试剂盒。还描述了用于监测蛋白质降解的内源替代标记，包括翻译后修饰（例如磷酸化）的丧失，例如，从受试者中除去细胞或组织; 和外源性分子前哨蛋白（例如连接到磁性纳米颗粒的磷酸蛋白），其允许评价细胞或组织群体样品的加工历史。

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