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1. 发明申请

WO2010151714A3 MOLECULAR ARRAYS 审中-公开
公开(公告)号：WO2010151714A3
公开(公告)日：2010-12-29
申请号：PCT/US2010/039882
申请日：2010-06-24
申请人： LIFE TECHNOLOGIES CORPORATION , ZHOU, Zhaohui , FRY, George A. , WOO, Sam Lee
发明人： ZHOU, Zhaohui , FRY, George A. , WOO, Sam Lee
IPC分类号： C12Q1/68 , G01N33/50
摘要： The disclosure relates generally to compositions, systems, and methods for use in preparing single molecule arrays, for example, arrays of single nucleic acid molecules, as well as arrays formed using such compositions, systems and methods by using a spacer which restricts the spacing between adjacent components of the array. In an exemplary embodiment, the array is formed by contacting a surface with a spacer-anchor complex including one or more spacers linked to an anchor, wherein the spacer comprises a linear or branched polymeric molecule folded to form a three-dimensional shape of desired size dimensions. The size of the folded spacer may optionally be adjusted to modulate the spacing distance between adjacent array components.

2. 发明公开

EP2721170A2 COMPOSITIONS AND METHODS FOR DETECTION OF CRONOBACTER SPP. AND CRONOBACTER SPECIES AND STRAINS 审中-公开
标题翻译：组合物和坂SPP的检测方法。与坂种和菌株
公开(公告)号：EP2721170A2
公开(公告)日：2014-04-23
申请号：EP12727766.3
申请日：2012-06-13
申请人： Life Technologies Corporation , Ji, Yongmei , Shih, Sheung-mei , Degoricija, Lovorka , Zhou, Zhaohui , Cummings, Craig , Furtado, Manohar , Brzoska, Pius , Burrell, Angela , Leong, Harrison , Fang, Xingwang , Bounpheng, Mangkey , Shah, Rohan
发明人： JI, Yongmei , SHIH, Sheung-Mei ("Rita") , DEGORICIJA, Lovorka , ZHOU, Zhaohui , CUMMINGS, Craig , FURTADO, Manohar , BRZOSKA, Pius , BURRELL, Angela , LEONG, Harrison , FANG, Xingwang , BOUNPHENG, Mangkey , SHAH, Rohan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12R1/01
摘要： Disclosed are genomic sequences for nine strains of C
ronobacter spp . (C.
sakazakii - 696, 701, 680; C.
malonaticus - 507, 681; C.
turicensis - 564; C.
muytjensii - 530; C.
dublinensis - 582; C.
genomospl - 581) and compositions, methods, and kits for detecting, identifying and distinguishing C
ronobacter spp . strains from each other and from non-C
ronobacter spp . strains. Some embodiments describe isolated nucleic acid compositions unique to certain C
ronobacter strains as well as compositions that are specific to all C
ronobacter spp . Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of C
ronobacter spp . are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR

3. 发明申请

WO2012174119A2 COMPOSITIONS AND METHODS FOR DETECTION OF CRONOBACTER SPP. AND CRONOBACTER SPECIES AND STRAINS 审中-公开
标题翻译：用于检测CRONOBACTER SPP的组合物和方法。和克隆物种和菌株
公开(公告)号：WO2012174119A2
公开(公告)日：2012-12-20
申请号：PCT/US2012/042263
申请日：2012-06-13
申请人： LIFE TECHNOLOGIES CORPORATION , JI, Yongmei , SHIH, Sheung-Mei ("Rita") , DEGORICIJA, Lovorka , ZHOU, Zhaohui , CUMMINGS, Craig , FURTADO, Manohar , BRZOSKA, Pius , BURRELL, Angela , LEONG, Harrison , FANG, Xingwang , BOUNPHENG, Mangkey , SHAH, Rohan
发明人： JI, Yongmei , SHIH, Sheung-Mei ("Rita") , DEGORICIJA, Lovorka , ZHOU, Zhaohui , CUMMINGS, Craig , FURTADO, Manohar , BRZOSKA, Pius , BURRELL, Angela , LEONG, Harrison , FANG, Xingwang , BOUNPHENG, Mangkey , SHAH, Rohan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12R1/01
摘要： Disclosed are genomic sequences for nine strains of C ronobacter spp . (C. sakazakii - 696, 701, 680; C. malonaticus - 507, 681; C. turicensis - 564; C. muytjensii - 530; C. dublinensis - 582; C. genomospl - 581) and compositions, methods, and kits for detecting, identifying and distinguishing C ronobacter spp . strains from each other and from non-C ronobacter spp . strains. Some embodiments describe isolated nucleic acid compositions unique to certain C ronobacter strains as well as compositions that are specific to all C ronobacter spp . Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of C ronobacter spp . are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR
摘要翻译：公开了九个克罗杆菌属菌株的基因组序列。（C. sakazakii-696,701,680; C. malonaticus-507,681; C. turicensis-564; C. muytjensii-530; C.dublinensis-582; C.基因组蛋白-58）和组合物，方法和试剂盒用于检测，识别和鉴别克罗杆菌属。菌株彼此和非克罗杆菌属。株。一些实施方案描述了某些克罗杆菌菌株特有的分离的核酸组合物以及对所有克罗杆菌属特异性的组合物。还提供引物和探针组合物以及引物和探针的使用方法。克隆杆菌属鉴定试剂盒也被描述。一些实施例涉及用于设置用于PCR分析的基于控制的阈值的计算机软件方法

4. 发明申请

WO2010141390A3 NUCLEOTIDE TRANSIENT BINDING FOR SEQUENCING METHODS 审中-公开
公开(公告)号：WO2010141390A3
公开(公告)日：2010-12-09
申请号：PCT/US2010/036755
申请日：2010-05-28
申请人： LIFE TECHNOLOGIES CORPORATION , VANDER HORN, Peter B. , CHEN, Cheng-Yao , NISHIMURA, Stefanie Yukiko , ZHOU, Zhaohui
发明人： VANDER HORN, Peter B. , CHEN, Cheng-Yao , NISHIMURA, Stefanie Yukiko , ZHOU, Zhaohui
IPC分类号： C12Q1/68 , C12N15/10 , C12N15/11 , G01N33/52
摘要： Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

5. 发明申请

WO2007087541A2 ASSAYS FOR S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES 审中-公开
标题翻译： S-腺嘌呤依赖性甲基衍生物的测定
公开(公告)号：WO2007087541A2
公开(公告)日：2007-08-02
申请号：PCT/US2007/060930
申请日：2007-01-23
申请人： WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATION , ZHOU, Zhaohui , DORGAN, Kathleen
发明人： ZHOU, Zhaohui , DORGAN, Kathleen
IPC分类号： C12Q1/52
CPC分类号： C12Q1/48 , C12Q1/52 , G01N2333/91011
摘要： Disclosed are novel methyltransferase assay methods, comprising: including, in a reaction mixture for a methyltransferase activity, a purified or recombinant adenosine nucleosidase activity that catalyses release of an adenine or adenine derivative moiety from a transmethylation product, and a purified or recombinant adenine deaminase activity that catalyses deamination of the released moiety to hypoxanthine or respective derivative and ammonia, wherein the methyltransferase activity is rate-limiting; and determining the methyltransferase activity by spectrophotometric or chromatographic monitoring of the coupled deamination reaction products, or of subsequent enzymatic or chemical reactions coupled thereto. Coupled oxidation of the hypoxanthine to uric acid and hydrogen peroxide is optionally affected using purified or recombinant xanthine oxidase, wherein the methyltransferase activity is rate-limiting, and wherein determining the methyltransferase activity comprises monitoring of the coupled oxidation reaction. Variations are disclosed comprises monitoring of reaction products (e.g., to detect NH3, Hypoxanthine, H2O2, and Uric Acid).
摘要翻译：公开了新的甲基转移酶测定方法，其包括：在用于甲基转移酶活性的反应混合物中，包含催化腺嘌呤或腺嘌呤衍生物部分从转甲基化产物释放的纯化或重组腺苷核苷酶活性，以及纯化或重组腺嘌呤脱氨酶活性催化释放的部分与次黄嘌呤或相应的衍生物和氨的脱氨基，其中甲基转移酶活性是速率限制的; 以及通过分光光度法或色谱监测偶联的脱氨基反应产物，或随后与其偶联的酶或化学反应来确定甲基转移酶活性。次黄嘌呤与尿酸和过氧化氢的偶联氧化任选地使用纯化或重组的黄嘌呤氧化酶来影响，其中甲基转移酶活性是速率限制的，并且其中确定甲基转移酶活性包括监测偶联的氧化反应。公开的变化包括监测反应产物（例如，检测NH 3，次黄嘌呤，H 2 O 2和尿酸）。

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