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1. 发明申请

WO2010151714A3 MOLECULAR ARRAYS 审中-公开
公开(公告)号：WO2010151714A3
公开(公告)日：2010-12-29
申请号：PCT/US2010/039882
申请日：2010-06-24
申请人： LIFE TECHNOLOGIES CORPORATION , ZHOU, Zhaohui , FRY, George A. , WOO, Sam Lee
发明人： ZHOU, Zhaohui , FRY, George A. , WOO, Sam Lee
IPC分类号： C12Q1/68 , G01N33/50
摘要： The disclosure relates generally to compositions, systems, and methods for use in preparing single molecule arrays, for example, arrays of single nucleic acid molecules, as well as arrays formed using such compositions, systems and methods by using a spacer which restricts the spacing between adjacent components of the array. In an exemplary embodiment, the array is formed by contacting a surface with a spacer-anchor complex including one or more spacers linked to an anchor, wherein the spacer comprises a linear or branched polymeric molecule folded to form a three-dimensional shape of desired size dimensions. The size of the folded spacer may optionally be adjusted to modulate the spacing distance between adjacent array components.

2. 发明申请

WO2011038158A2 METHODS, COMPOSITIONS, SYSTEMS AND APPARATUS FOR MOLECULAR ARRAY FABRICATION 审中-公开
标题翻译：用于分子阵列制造的方法，组合物，系统和装置
公开(公告)号：WO2011038158A2
公开(公告)日：2011-03-31
申请号：PCT/US2010/050070
申请日：2010-09-23
申请人： LIFE TECHNOLOGIES CORPORATION , FRY, George A. , WOO, Sam Lee , INMAN, Christina E.
发明人： FRY, George A. , WOO, Sam Lee , INMAN, Christina E.
IPC分类号： G01N33/50 , C12Q1/68 , G01N33/543 , B81C1/00
CPC分类号： C40B50/14 , B01J19/0046 , B01J2219/00466 , B01J2219/005 , B01J2219/00527 , B01J2219/00576 , B01J2219/00596 , B01J2219/00648 , B01J2219/00668 , B01J2219/00722 , C40B50/18
摘要： Provided herein are compositions, methods, systems and apparatuses for use in preparing arrays of molecules or other compounds. The systems include beads which are linked to the molecules or other compounds to be arrayed. The arrays are prepared by depositing the bead-molecule complexes onto a surface. The bead-molecule complexes can be used to prepare an array of single molecules, where each single molecule is attached to a surface at a single location on the surface. In one exemplary embodiment, the molecules to be deposited can include nucleic acid.
摘要翻译：本文提供了用于制备分子阵列或其他化合物的组合物，方法，系统和装置。该系统包括与分子或其他要排列的化合物相连的珠子。阵列通过将珠子分子复合物沉积到表面上来制备。珠子分子复合物可用于制备单个分子阵列，其中每个单个分子附着到表面上单个位置的表面。在一个示例性实施方案中，待沉积的分子可以包括核酸。

3. 发明申请

WO2012058647A1 CHEMICALLY-ENHANCED PRIMER COMPOSITIONS, METHODS AND KITS 审中-公开
标题翻译：化学增强的组合物，方法和工具
公开(公告)号：WO2012058647A1
公开(公告)日：2012-05-03
申请号：PCT/US2011/058474
申请日：2011-10-28
申请人： LIFE TECHNOLOGIES CORPORATION , LEE, Linda , WOO, Sam Lee , MA, Peter
发明人： LEE, Linda , WOO, Sam Lee , MA, Peter
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125
摘要： A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
摘要翻译：提供了一种组合物，其包含带负电荷的基团，寡核苷酸序列和至少一个或一个核酸酶抗性连锁基团以形成化学增强的引物。化学增强引物可用于测序和片段分析。还提供了合成引物的方法以及用于测序的DNA的制备方法和测序DNA的方法以及含有化学增强的引物的试剂盒。 DNA测序的方法可以包括使扩增反应产物与组合物在过量扩增引物被核酸酶降解的条件下接触，化学增强引物基本上不降解。

4. 发明申请

WO2005054515A2 NOVEL METHOD FOR ISOLATING SINGLE STRANDED PRODUCT 审中-公开
标题翻译：隔离单条产品的新方法
公开(公告)号：WO2005054515A2
公开(公告)日：2005-06-16
申请号：PCT/US2004/039700
申请日：2004-11-24
申请人： APPLERA CORPORATION , DIMSOSKI, Pero , WOO, Sam Lee
发明人： DIMSOSKI, Pero , WOO, Sam Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6853 , C12Q2531/113 , C12Q2525/197
摘要： The present teachings relate to methods of purifying, isolating, separating, and identifying target nucleic acids. In some embodiments of the present teachings, an affinity moiety can be incorporated into one of the flanking primers of a nucleic acid amplification reaction primer pair. The reaction mixture can be contacted with a binding moiety specific for the affinity moiety, thereby allowing immobilization of the double stranded amplification product, separation of reaction components lacking the affinity moiety, and isolation of the target nucleic acid strand. Further, one of the flanking primers of the nucleic acid amplification reaction can comprise a label and a mobility modifier, thereby facilitating identification of the target nucleic acids. In some embodiments, the amplification reaction is multiplexed and comprises polymorphic microsatellites useful in human identification and the manufacturing of molecular size standards. Some embodiments of the present teachings provide for improved methods of performing electrokinetic injection.
摘要翻译：本教导涉及纯化，分离，分离和鉴定靶核酸的方法。在本教导的一些实施方案中，可将亲和部分并入核酸扩增反应引物对的侧翼引物之一中。反应混合物可以与对亲和部分特异的结合部分接触，由此允许固定双链扩增产物，分离缺乏亲和部分的反应组分和分离靶核酸链。此外，核酸扩增反应的侧翼引物之一可以包含标记和迁移率调节剂，从而有助于鉴定靶核酸。在一些实施方案中，扩增反应是多路复用的并且包含可用于人类鉴定和制造分子大小标准物的多态微卫星。本教导的一些实施例提供了进行电动注射的改进方法。

5. 发明申请

WO2007024914A3 DEVICE AND METHOD FOR MICROFLUIDIC CONTROL OF A FIRST FLUID IN CONTACT WITH A SECOND FLUID, WHEREIN THE FIRST AND SECOND FLUIDS ARE IMMISCIBLE 审中-公开
公开(公告)号：WO2007024914A3
公开(公告)日：2007-03-01
申请号：PCT/US2006/032861
申请日：2006-08-22
申请人： APPLERA CORPORATION , COX, David M. , WOO, Sam Lee , BOEGE, Steven J. , OLDHAM, Mark F. , REEL, Richard T. , NORDMAN, Eric S. , LEE, Linda G. , WIYATNO, Willy , SAGATELYAN, Dmitry M.
发明人： COX, David M. , WOO, Sam Lee , BOEGE, Steven J. , OLDHAM, Mark F. , REEL, Richard T. , NORDMAN, Eric S. , LEE, Linda G. , WIYATNO, Willy , SAGATELYAN, Dmitry M.
IPC分类号： F15C3/00
摘要： Various embodiments described in the application relate to an apparatus, system, and method for fluidically controlling, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise combinations of detectors, controllers, valves, and fluid supply units to control the spatial size, location and/or fluidic composition of discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

6. 发明公开

EP2633076A1 CHEMICALLY-ENHANCED PRIMER COMPOSITIONS, METHODS AND KITS 有权转让
标题翻译：化学增强底漆组合物，方法和试剂盒
公开(公告)号：EP2633076A1
公开(公告)日：2013-09-04
申请号：EP11785838.1
申请日：2011-10-28
申请人： Life Technologies Corporation
发明人： LEE, Linda , WOO, Sam Lee , MA, Peter
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125
摘要： A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

7. 发明授权

EP2633076B1 CHEMICALLY-ENHANCED PRIMER COMPOSITIONS, METHODS AND KITS 有权转让
标题翻译：化学增强底漆组合物，方法和试剂盒
公开(公告)号：EP2633076B1
公开(公告)日：2016-07-27
申请号：EP11785838.1
申请日：2011-10-28
申请人： Life Technologies Corporation
发明人： LEE, Linda , WOO, Sam Lee , MA, Peter
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125

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