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1. 发明申请

WO2011082419A3 DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME 审中-公开
标题翻译： DNA序列方法和检测器及其执行系统
公开(公告)号：WO2011082419A3
公开(公告)日：2012-02-09
申请号：PCT/US2011020117
申请日：2011-01-04
申请人： LIFE TECHNOLOGIES CORP , SUN HONGYE , NORDMAN ERIC S , OLDHAM MARK F , O'NEILL JOHN R , CONNELL CHARLES , ULMANELLA UMBERTO , LAU ALDRICH N K , KOTSEROGLOU THEOFILOS , LIVAK KENNETH J
发明人： SUN HONGYE , NORDMAN ERIC S , OLDHAM MARK F , O'NEILL JOHN R , CONNELL CHARLES , ULMANELLA UMBERTO , LAU ALDRICH N K , KOTSEROGLOU THEOFILOS , LIVAK KENNETH J
IPC分类号： C12Q1/68 , G01N27/414 , G01N33/50
CPC分类号： C12Q1/6869 , B01L3/502761 , G01N27/3278 , G01N27/414 , G01N27/4145 , G01N27/4146 , G01N33/5438 , Y10S977/924 , Y10T436/143333 , C12Q2565/631
摘要： In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.
摘要翻译：在一些实施例中，提供了分析物检测系统，其包括纳米通道，电极装置和设置在纳米通道中的多个纳米器件。可以使用多个核酸碱基检测组分，其包括多个纳米孔，多个纳米通道，多个杂交探针及其组合等。根据本教导的其他实施方案，将不同的编码分子与靶DNA分子杂交并用于检测沿着靶分子的各种序列的存在。还提供了包含编码分子混合物的试剂盒。在一些实施方案中，包括纳米通道，纳米孔等的装置用于操纵DNA分子的移动，例如用于DNA测序检测的准备。还提供了制备其的纳米孔结构及其制备方法，使用纳米孔结构的核酸测序方法也是如此。提供表面改性的纳米孔也是制备它们的方法。在一些实施方案中，提供用于减缓单链DNA（ssDNA）穿过纳米孔的易位的表面改性的纳米孔，以及配置成检测ssDNA链上的多个不同碱基中的每一个的纳米孔。

2. 发明申请

WO2008036697A3 METHODS, SYSTEMS AND APPARATUS FOR LIGHT CONCENTRATING MECHANISMS 审中-公开
标题翻译：用于光浓缩机制的方法，系统和装置
公开(公告)号：WO2008036697A3
公开(公告)日：2008-08-07
申请号：PCT/US2007078812
申请日：2007-09-18
申请人： APPLERA CORP , OLDHAM MARK F , NORDMAN ERIC S , CONNELL CHARLES R , WOUDENBERG TIMOTHY M
发明人： OLDHAM MARK F , NORDMAN ERIC S , CONNELL CHARLES R , WOUDENBERG TIMOTHY M
IPC分类号： G01N33/00
CPC分类号： G01N21/648 , G01N2021/6432
摘要： An embodiment relates generally to a method for analysis of a nucleic acid. The method includes providing for a resonant structure configured to couple with one or more fluorescently labeled nucleic acids and directing an excitation light from a source on the resonant structure. The method also includes generating plasmons on the surface of the resonant structure where the analyte is fixed at a point of energy concentration of the resonant structure.
摘要翻译：实施方案一般涉及核酸分析方法。该方法包括提供构造成与一个或多个荧光标记的核酸耦合并将来自源的激发光引导到谐振结构上的谐振结构。该方法还包括在谐振结构的表面上产生等离子体激元，其中分析物固定在谐振结构的能量集中点处。

3. 发明申请

WO2008036697A2 METHODS, SYSTEMS AND APPARATUS FOR LIGHT CONCENTRATING MECHANISMS 审中-公开
标题翻译：用于光聚集机构的方法，系统和设备
公开(公告)号：WO2008036697A2
公开(公告)日：2008-03-27
申请号：PCT/US2007078812
申请日：2007-09-18
申请人： APPLERA CORP , OLDHAM MARK F , NORDMAN ERIC S , CONNELL CHARLES R , WOUDENBERG TIMOTHY M
发明人： OLDHAM MARK F , NORDMAN ERIC S , CONNELL CHARLES R , WOUDENBERG TIMOTHY M
IPC分类号： G01N33/00
CPC分类号： G01N21/648 , G01N2021/6432
摘要： An embodiment relates generally to a method for analysis of a nucleic acid. The method includes providing for a resonant structure configured to couple with one or more fluorescently labeled nucleic acids and directing an excitation light from a source on the resonant structure. The method also includes generating plasmons on the surface of the resonant structure where the analyte is fixed at a point of energy concentration of the resonant structure.
摘要翻译：一个实施方案一般涉及分析核酸的方法。所述方法包括提供谐振结构，所述谐振结构被配置成与一种或多种荧光标记的核酸偶联并且将来自源的激发光引导到谐振结构上。该方法还包括在谐振结构的表面上产生等离子体，其中分析物固定在谐振结构的能量集中点处。

4. 发明申请

WO2007143536A3 DEVICES AND METHODS FOR CONTROLLING BUBBLE FORMATION IN MICROFLUIDIC DEVICES 审中-公开
标题翻译：用于控制微流体装置中的气泡形成的装置和方法
公开(公告)号：WO2007143536A3
公开(公告)日：2008-01-31
申请号：PCT/US2007070197
申请日：2007-06-01
申请人： APPLERA CORP , ULMANELLA UMBERTO , NORDMAN ERIC S , SONG MAENGSEOK , YANG JOON MO , LEE JULIE C , BEARD NIGEL P , YUE MIN , SCHEMBRI CAROL , LIU DAVID M
发明人： ULMANELLA UMBERTO , NORDMAN ERIC S , SONG MAENGSEOK , YANG JOON MO , LEE JULIE C , BEARD NIGEL P , YUE MIN , SCHEMBRI CAROL , LIU DAVID M
IPC分类号： F16K31/44 , C02F1/40
CPC分类号： B01L3/502746 , B01L3/502723 , B01L3/502784 , B01L2200/0642 , B01L2200/0673 , B01L2200/0684 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , B01L2400/086
摘要： A microfluidic device may include a sample distribution network including a plurality of sample chambers configured to be loaded with biological sample for biological testing of the biological sample while in the sample chambers. The sample distribution network may further include a plurality of inlet channels, each inlet channel being in flow communication with and configured to flow biological sample to a respective sample chamber, and a plurality of outlet channels, each outlet channel being in flow communication and configured to flow biological sample from a respective sample chamber. At least some of the sample chambers may include a physical modification configured to control the movement of the meniscus so as to control bubble formation or dried reagent positioned within the at least some sample chambers proximate the inlet channels in flow communication with the at least some sample chambers.
摘要翻译：微流体装置可以包括样品分配网络，其包括多个样品室，其被配置为装载用于在样品室中的生物样品的生物测试的生物样品。样品分配网络还可以包括多个入口通道，每个入口通道与生物样品流动连通并配置成将生物样品流动到相应的样品室，以及多个出口通道，每个出口通道处于流动连通状态并被配置为从相应的样品室流动生物样品。至少一些样品室可以包括物理修饰，其被配置为控制弯液面的运动，以便控制气泡形成或位于靠近入口通道的至少一些样品室内的干燥试剂，其与至少一些样品室。

5. 发明申请

WO2007024914A2 DEVICE AND METHOD FOR MICROFLUIDIC CONTROL OF A FIRST FLUID IN CONTACT WITH A SECOND FLUID, WHEREIN THE FIRST AND SECOND FLUIDS ARE IMMISCIBLE 审中-公开
标题翻译：第一流体与第二流体接触的第一流体的微流控制的装置和方法，其中第一和第二流体是不容易的
公开(公告)号：WO2007024914A2
公开(公告)日：2007-03-01
申请号：PCT/US2006032861
申请日：2006-08-22
申请人： APPLERA CORP , COX DAVID M , WOO SAM LEE , BOEGE STEVEN J , OLDHAM MARK F , REEL RICHARD T , NORDMAN ERIC S , LEE LINDA G , WIYATNO WILLY , SAGATELYAN DMITRY M
发明人： COX DAVID M , WOO SAM LEE , BOEGE STEVEN J , OLDHAM MARK F , REEL RICHARD T , NORDMAN ERIC S , LEE LINDA G , WIYATNO WILLY , SAGATELYAN DMITRY M
IPC分类号： B01D24/00
CPC分类号： C12Q1/6869 , B01J2219/00353 , B01J2219/0036 , B01J2219/00364 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01L3/0293 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , C12Q1/6874 , F15C5/00 , F16K99/0001 , F16K99/0011 , F16K99/0013 , F16K2099/0084 , G01N1/14 , G01N27/44743 , G01N27/44769 , G01N35/08 , Y10T137/0318 , Y10T137/4259 , Y10T137/85978 , Y10T137/85986 , Y10T137/86863 , Y10T436/2575 , C12Q2535/101
摘要： Various embodiments described in the application relate to an apparatus, system, and method for fluidically controlling, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise combinations of detectors, controllers, valves, and fluid supply units to control the spatial size, location and/or fluidic composition of discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.
摘要翻译：在本申请中描述的各种实施例涉及用于在管道内流体控制与第二流体不混溶的一种或多种流体的离散体积的装置，系统和方法。离散体积可用于涉及小体积的生物化学或分子生物学过程，例如微升体积，纳升级体积或更小体积。该系统可以包括检测器，控制器，阀和流体供应单元的组合，以控制通过与离散的流体不混溶的流体彼此分离的离散体积的空间尺寸，位置和/或流体组成体积，例如由油分离的含水不混溶流体离散体积。

6. 发明申请

WO2006135782A2 METHOD AND SYSTEM FOR MULTIPLEX GENETIC ANALYSIS 审中-公开
标题翻译：多基因遗传分析方法与系统
公开(公告)号：WO2006135782A2
公开(公告)日：2006-12-21
申请号：PCT/US2006022545
申请日：2006-06-09
申请人： APPLERA CORP , NORDMAN ERIC S , OLDHAM MARK F , WOUDENBERG TIMOTHY
发明人： NORDMAN ERIC S , OLDHAM MARK F , WOUDENBERG TIMOTHY
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , G01N21/6428 , G01N21/6452 , G01N21/648 , G01N21/6486 , G01N2021/6441 , C12Q2563/107
摘要： The present disclosure provides apparatus, systems and method for detecting separately and substantially simultaneously light emissions from a plurality of localized light-emitting analytes. A system according to exemplary embodiments of the present disclosure comprises a sample holder having structures formed thereon for spatially separating and constraining a plurality of light-emitting analytes each having a single nucleic acid molecule or a single nucleic acid polymerizing enzyme, a light source configured to illuminate the sample holder, an optical assembly configured to collect and detect separately and substantially simultaneously light emissions associated with the plurality of light emitting analytes. The system may further include a computer system configured to analyze the light emissions to determine the structures or properties of a target nucleic acid molecule associated with each analyte.
摘要翻译：本公开提供了用于单独和基本上同时检测来自多个局部发光分析物的光发射的装置，系统和方法。根据本公开的示例性实施例的系统包括具有形成在其上的结构的样本保持器，用于空间分离和约束各自具有单个核酸分子或单个核酸聚合酶的多个发光分析物，光源被配置为照亮样品架，光学组件被配置成分别收集和检测与多个发光分析物相关联的基本上同时的发光。该系统还可以包括被配置为分析光发射以确定与每个分析物相关联的靶核酸分子的结构或性质的计算机系统。

7. 发明申请

WO2005008235A3 ELECTROPHORETIC SYSTEM WITH MULTI-NOTCH FILTER AND LASER EXCITATION SOURCE 审中-公开
标题翻译：具有多孔过滤器和激光源的电泳系统
公开(公告)号：WO2005008235A3
公开(公告)日：2005-08-18
申请号：PCT/US2004022202
申请日：2004-07-08
申请人： APPLERA CORP , OLDHAM MARK F , NORDMAN ERIC S , REEL RICHARD T , SHIGEURA JANICE G LF
发明人： OLDHAM MARK F , NORDMAN ERIC S , REEL RICHARD T , SHIGEURA JOHN S DI
IPC分类号： G01N15/14 , G01N27/447 , G02B5/28
CPC分类号： G01N15/1475 , B01D57/02 , G01N27/44721 , G01N2015/144 , G01N2015/149
摘要： Apparatus and methods for detecting analytes in a sample are provided. The apparatus includes: one or more channels having a dectection zone (14); one or more irradiation sources (16) disposed for irradiating the detection zone (14) with radiation; a detector (34) capable of collecting at least one chage corresponding to an emission beam (22) emitted from the detection zone (14), the detector (34) having an output; and a time delay integration system coupled to the detector for effecting time delay integration of the at least one charge by accumulating the at least one charge before reading the at least one charge at the output of the detector. Various embodiments provide an apparatus with a multi-notch filter. Various embodiments provide a solid state laser, a micro-wire laser, or an organic light-emitting diode as a irradiation source.
摘要翻译：提供了用于检测样品中分析物的装置和方法。该装置包括：一个或多个通道，具有检测区域（14）; 一个或多个辐射源（16）被设置用于用辐射照射检测区域（14）; 检测器（34），其能够收集对应于从所述检测区域（14）发射的发射光束（22）的至少一个机器，所述检测器（34）具有输出; 以及耦合到所述检测器的时间延迟积分系统，用于通过在所述检测器的输出读取所述至少一个电荷之前积累所述至少一个电荷来实现所述至少一个电荷的时间延迟积分。各种实施例提供具有多陷波滤波器的装置。各种实施例提供固态激光器，微线激光器或有机发光二极管作为照射源。

8. 发明申请

WO2009006445A2 SYSTEMS AND METHODS FOR ELECTRONIC DETECTION WITH NANOFETS 审中-公开
标题翻译：具有纳米垫的电子检测系统和方法
公开(公告)号：WO2009006445A2
公开(公告)日：2009-01-08
申请号：PCT/US2008068857
申请日：2008-06-30
申请人： APPLIED BIOSYSTEMS , OLDHAM MARK F , NORDMAN ERIC S
发明人： OLDHAM MARK F , NORDMAN ERIC S
IPC分类号： C12Q1/68
CPC分类号： G01N33/54373 , C12Q1/6825 , G01N27/4145 , G01N27/4146 , C12Q2563/155 , C12Q2563/113
摘要： There is disclosed a system for electrical charge detection comprising a nanoFET device. Also disclosed is a method of electrical charge detection for single molecule sequencing. The method includes attaching a macromolecule or assemblies thereof to a gate of a nanoFET device and flowing in a solution of charge tags, where a charge tag includes a nucleotide attached to a charge complex. The method also includes incorporating one charge tag into the macromolecule or assemblies thereof and cleaving the charge tags from the macromolecule or assemblies thereof. The method further includes detecting at least one of current and voltage from the nanoFET device.
摘要翻译：公开了一种包括nanoFET器件的用于电荷检测的系统。还公开了用于单分子测序的电荷检测方法。该方法包括将大分子或其组件附着到纳米FET器件的栅极并且在电荷标签的溶液中流动，其中电荷标签包括附接到电荷复合物的核苷酸。该方法还包括将一个电荷标签结合到大分子或其组件中，并从大分子或其组件中切割电荷标签。该方法还包括检测来自纳米FET器件的电流和电压中的至少一个。

9. 发明申请

WO2012166742A2 SYSTEMS AND METHODS FOR GENETIC AND BIOLOGICAL ANALYSIS 审中-公开
标题翻译：遗传与生物学分析系统与方法
公开(公告)号：WO2012166742A2
公开(公告)日：2012-12-06
申请号：PCT/US2012039880
申请日：2012-05-29
申请人： GENAPSYS INC , ESFANDYARPOUR HESAAM , BAGHBANI PARAZI KOSAR , OLDHAM MARK F , NORDMAN ERIC S , REEL RICHARD T , BAUMGARTNER SUSANNE , HEINER CHERYL , LEE FRANK
发明人： ESFANDYARPOUR HESAAM , BAGHBANI PARAZI KOSAR , OLDHAM MARK F , NORDMAN ERIC S , REEL RICHARD T , BAUMGARTNER SUSANNE , HEINER CHERYL , LEE FRANK
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6825 , C12Q1/6874 , C12Q2549/126 , C12Q2563/155 , C12Q2563/116 , C12Q2563/149 , C12Q2565/607 , C12Q2535/122
摘要： The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.
摘要翻译：本发明涉及用于测序多核苷酸的系统和方法，以及检测涉及其它生物分子的反应和结合事件。该系统和方法可以采用无室装置和纳米传感器来检测或表征高通量的这种反应。因为在许多实施例中的系统是可重复使用的，与单次使用设备相比，该系统可以经受更复杂和改进的工程。

10. 发明申请

WO2007150043A3 SYSTEMS AND METHODS FOR COOLING IN A THERMAL CYCLER 审中-公开
标题翻译：用于在热循环中冷却的系统和方法
公开(公告)号：WO2007150043A3
公开(公告)日：2008-09-12
申请号：PCT/US2007071930
申请日：2007-06-22
申请人： APPLERA CORP , OLDHAM MARK F , HENDERSON ERIC G , MIRCHANDANI VINOD , NORDMAN ERIC S , HABERSTROH MARC , SLUIS JOHANNES PAUL
发明人： OLDHAM MARK F , HENDERSON ERIC G , MIRCHANDANI VINOD , NORDMAN ERIC S , HABERSTROH MARC , SLUIS JOHANNES PAUL
IPC分类号： C12M1/36
CPC分类号： B01L7/52 , B01L2300/0636 , B01L2300/0829 , B01L2300/0877 , B01L2300/1822 , B01L2300/1844 , B01L2300/185
摘要： A device for performing polymerase chain reactions using cooling members not used in conventional thermal cyclers. A device for performing polymerase chain reactions may include a sample holder configured to receive a nucleic acid sample, a heating system configured to raise the temperature of the sample, a cooling system configured to lower the temperature of the sample, and a controller configured to operably control the heating system and the cooling system to cycle the device through a desired time-temperature profile. The cooling system may comprise at least one cooling member selected from a synthetic jet ejector array, a vibration-induced droplet atomization system, a vibrating diaphragm system, a piezo fan, a Cold Gun, a microchannel cooling loop, and a Cool Chip.
摘要翻译：使用不用于常规热循环仪的冷却部件进行聚合酶链反应的装置。用于进行聚合酶链式反应的装置可以包括配置成接收核酸样品的样品架，配置成提高样品温度的加热系统，配置成降低样品温度的冷却系统，以及配置成可操作地控制加热系统和冷却系统以使设备循环所需的时间 - 温度曲线。冷却系统可以包括选自合成射流喷射器阵列，振动诱导液滴雾化系统，振动膜系统，压电风扇，冷枪，微通道冷却回路和冷却芯片中的至少一个冷却构件。

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