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1. 发明申请

WO2009031766A2 MUTANTS HAVING CAPABILITY TO PRODUCE 1,4-BUTANEDIOL AND METHOD FOR PREPARING 1,4-BUTANEDIOL USING THE SAME 审中-公开
标题翻译：具有生产1,4-丁二醇能力的突变体以及使用该方法制备1,4-丁二醇的方法
公开(公告)号：WO2009031766A2
公开(公告)日：2009-03-12
申请号：PCT/KR2008/004700
申请日：2008-08-13
申请人： LG CHEM, LTD. , KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , PARK, Si Jae , LEE, Sang Hyun , LEE, Sang Yup , LEE, Eun Jeong
发明人： PARK, Si Jae , LEE, Sang Hyun , LEE, Sang Yup , LEE, Eun Jeong
IPC分类号： C12N15/10
CPC分类号： C12N15/77 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/13 , C12P7/18 , C12Y101/01061 , C12Y102/01024 , C12Y103/99002
摘要： A mutant capable of producing 1,4-butanediol and a method of preparing 1,4- butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4- hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.
摘要翻译：提供了能够生产1,4-丁二醇的突变体和使用该突变体制备1,4-丁二醇的方法。通过在能够产生琥珀酸的微生物中引入和扩增编码将琥珀酸转化成4-羟基丁酸和4-羟基丁酸的酶的基因并扩增到1,4-丁二醇中来制备突变体微生物。该方法包括在含有碳水化合物的培养基中培养突变体并从培养物中获得1,4-丁二醇。因此，化学工业中必不可少的1,4-丁二醇可以用生物过程制备。

2. 发明授权

EP2201115B1 MUTANTS HAVING CAPABILITY TO PRODUCE 1,4-BUTANEDIOL AND METHOD FOR PREPARING 1,4-BUTANEDIOL USING THE SAME 有权
标题翻译：突变体的能力生产1,4-丁二醇和用于生产1,4-丁二醇THEREOF USING
公开(公告)号：EP2201115B1
公开(公告)日：2012-10-24
申请号：EP08793214.1
申请日：2008-08-13
申请人： LG Chem, Ltd. , Korea Advanced Institute of Science and Technology
发明人： PARK, Si Jae , LEE, Sang Hyun , LEE, Sang Yup , LEE, Eun Jeong
IPC分类号： C12N15/10
CPC分类号： C12N15/77 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/13 , C12P7/18 , C12Y101/01061 , C12Y102/01024 , C12Y103/99002

3. 发明公开

EP2201115A2 MUTANTS HAVING CAPABILITY TO PRODUCE 1,4-BUTANEDIOL AND METHOD FOR PREPARING 1,4-BUTANEDIOL USING THE SAME 有权
标题翻译：突变体的能力生产1,4-丁二醇和用于生产1,4-丁二醇THEREOF USING
公开(公告)号：EP2201115A2
公开(公告)日：2010-06-30
申请号：EP08793214.1
申请日：2008-08-13
申请人： LG Chem, Ltd. , Korea Advanced Institute of Science and Technology
发明人： PARK, Si Jae , LEE, Sang Hyun , LEE, Sang Yup , LEE, Eun Jeong
IPC分类号： C12N15/10
CPC分类号： C12N15/77 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/13 , C12P7/18 , C12Y101/01061 , C12Y102/01024 , C12Y103/99002
摘要： A mutant capable of producing 1,4-butanediol and a method of preparing 1,4- butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4- hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.

4. 发明授权

EP2201115B2 MUTANTS HAVING CAPABILITY TO PRODUCE 1,4-BUTANEDIOL AND METHOD FOR PREPARING 1,4-BUTANEDIOL USING THE SAME 有权
标题翻译：突变体的能力生产1,4-丁二醇和用于生产1,4-丁二醇THEREOF USING
公开(公告)号：EP2201115B2
公开(公告)日：2016-07-20
申请号：EP08793214.1
申请日：2008-08-13
申请人： LG Chem, Ltd. , Korea Advanced Institute of Science and Technology
发明人： PARK, Si Jae , LEE, Sang Hyun , LEE, Sang Yup , LEE, Eun Jeong
IPC分类号： C12N15/10
CPC分类号： C12N15/77 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/13 , C12P7/18 , C12Y101/01061 , C12Y102/01024 , C12Y103/99002

5. 发明申请

WO2009022797A1 MUTANT OF PROPIONYL-COA TRANSFERASE FROM CLOSTRIDIUM PROPIONICUM AND PREPARING METHOD FOR PLA OR PLA COPOLYMER USING THE SAME 审中-公开
标题翻译：丙酮酸丙酮转移酶及其制备方法的PLA / PLA共聚物的制备方法
公开(公告)号：WO2009022797A1
公开(公告)日：2009-02-19
申请号：PCT/KR2008/004348
申请日：2008-07-25
申请人： LG CHEM, LTD. , PARK, Si Jae , YANG, Taek Ho , KANG, Hye Ok , LEE, Sang Hyun , LEE, Eun Jung , KIM, Tae Wan
发明人： PARK, Si Jae , YANG, Taek Ho , KANG, Hye Ok , LEE, Sang Hyun , LEE, Eun Jung , KIM, Tae Wan
IPC分类号： C12N15/54
CPC分类号： C12P7/625 , C12N9/1029 , C12N9/1048 , C12N9/13 , C12Y203/01 , C12Y208/03001 , Y02P20/52
摘要： Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E.coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E.coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.
摘要翻译：本发明提供一种丙酸酰辅酶A转移酶突变体，其可从丙酸梭菌中，在使用微生物制备聚乳酸（PLA）或PLA共聚物的方法中，可以高效地将乳酸转化为乳酰辅酶A。与在大肠杆菌中弱表达的常规丙酰基-CoA转移酶不同，当将来自丙酸梭菌的丙酰辅酶A转移酶的突变体引入重组大肠杆菌中时，可以非常顺利地提供乳酰辅酶A，从而能够高效制备聚乳酸（PLA）和PLA共聚物。

6. 发明申请

WO2009091141A3 RECOMBINANT MICROORGANISM HAVING A PRODUCING ABILITY OF POLYLACTATE OR ITS COPOLYMERS AND METHOD FOR PREPARING POLYLACTATE OR ITS COPOLYMERS USING THE SAME 审中-公开
公开(公告)号：WO2009091141A3
公开(公告)日：2009-07-23
申请号：PCT/KR2008/007790
申请日：2008-12-30
申请人： LG CHEM, LTD. , PARK, Si Jae , YANG, Taek Ho , LEE, Sang Hyun , LEE, Eun Jung , KANG, Hye Ok , KIM, Tae Wan
发明人： PARK, Si Jae , YANG, Taek Ho , LEE, Sang Hyun , LEE, Eun Jung , KANG, Hye Ok , KIM, Tae Wan
IPC分类号： C12N15/31
摘要： Provided are a recombinant microorganism capable of producing polylactate (PLA) or hydroxyalkanoate-lactate copolymers and a method of preparing PLA or hydroxyalkanoate-lactate copolymers using the same. The recombinant microorganism has both a gene encoding a propionyl-CoA transferase from Megasphaera elsdenii and a gene encoding a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate. A propionyl-CoA transferase from Megasphaera elsdenii is introduced into the recombinant microorganism to effectively provide lactyl-CoA, thereby enabling efficient preparation of PLA or PLA copolymers.

7. 发明申请

WO2009020279A1 PREPARING METHOD FOR (S)-3-HYDROXYBUTYRIC ACID AND (S)-3-HYDROXYBUTYRATE ESTER USING RECOMBINANT MICROORGANISM 审中-公开
标题翻译：（S）-3-羟基丁酸和（S）-3-羟基丁酸酯的制备方法使用重组微生物
公开(公告)号：WO2009020279A1
公开(公告)日：2009-02-12
申请号：PCT/KR2008/003217
申请日：2008-06-10
申请人： LG CHEM, LTD. , PARK, Si Jae , LEE, Sang Hyun
发明人： PARK, Si Jae , LEE, Sang Hyun
IPC分类号： C12N15/66
CPC分类号： C12P7/62 , C12P7/42
摘要： A method of synthesizing optically-active (S)-3-hydroxybutyric acid and (S)-3-hydroxybutyrate ester using a mutated microorganism is provided. More particularly, a mutated microorganism for preparing (S)-3-hydroxybutyric acid transformed with a gene encoding β-ketothiolase, a gene encoding (S)-3- hydroxybutyryl CoA dehydrogenase and a gene encoding acyl CoA hydrolase; a method of preparing (S)-3-hydroxybutyric acid using the mutated microorganism; a mutated microorganism for preparing (S)-3-hydroxybutyrate ester transformed with a gene encoding β-ketothiolase, a gene encoding (S)- 3-hydroxybutyryl CoA dehydrogenase, a gene encoding acyl CoA hydrolase and a gene encoding lipase; and a method of preparing (S)-3-hydroxybutyrate ester using the mutated microorganism are provided. Accordingly, (S)-3-hydroxybutyric acid with high optical purity may be produced from acetyl CoA produced in glycolysis of a microorganism by a simple process involving the manipulation of a metabolic pathway by a recombinant gene introduced into the microorganism without using a high-cost metal catalyst or a substrate. Further, (S)-3-hydroxybutyrate ester and lactone of (S)-3-hydroxybutyrate ester may be simply produced from (S)-3- hydroxybutyric acid produced by the above method using lipase.
摘要翻译：提供了使用突变微生物合成光学活性（S）-3-羟基丁酸和（S）-3-羟基丁酸酯的方法。更具体地，涉及用编码β-酮硫解酶的基因，编码（S）-3-羟基丁酰辅酶A脱氢酶的基因和编码酰基辅酶A水解酶的基因转化的（S）-3-羟基丁酸的突变微生物; 使用突变的微生物制备（S）-3-羟基丁酸的方法; 用于制备用编码β-酮硫解酶的基因，编码（S）-3-羟基丁酰辅酶A脱氢酶的基因，编码酰基辅酶A水解酶的基因和编码脂肪酶的基因转化的（S）-3-羟基丁酸酯的突变微生物; 并提供使用突变微生物制备（S）-3-羟基丁酸酯的方法。因此，具有高光学纯度的（S）-3-羟基丁酸可以通过涉及通过引入微生物的重组基因操纵代谢途径的简单方法由乙醇辅酶A生产的乙酰辅酶A产生，成本金属催化剂或基材。此外，（S）-3-羟基丁酸酯的（S）-3-羟基丁酸酯和内酯可以由使用脂肪酶的上述方法制备的（S）-3-羟基丁酸简单地制备。

8. 发明公开

EP2188375A1 MUTANT OF PROPIONYL-COA TRANSFERASE FROM CLOSTRIDIUM PROPIONICUM AND PREPARING METHOD FOR PLA OR PLA COPOLYMER USING THE SAME 有权
标题翻译：丙酰辅酶A转移酶突变体从丙酸梭菌及其制备方法PLA或PLA共聚物SO
公开(公告)号：EP2188375A1
公开(公告)日：2010-05-26
申请号：EP08792894.1
申请日：2008-07-25
申请人： LG Chem, Ltd.
发明人： PARK, Si Jae , YANG, Taek Ho , KANG, Hye Ok , LEE, Sang Hyun , LEE, Eun Jung , KIM, Tae Wan
IPC分类号： C12N15/54
CPC分类号： C12P7/625 , C12N9/1029 , C12N9/1048 , C12N9/13 , C12Y203/01 , C12Y208/03001 , Y02P20/52
摘要： Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E.coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E.coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.

9. 发明授权

EP2188375B1 MUTANT OF PROPIONYL-COA TRANSFERASE FROM CLOSTRIDIUM PROPIONICUM AND PREPARING METHOD FOR PLA OR PLA COPOLYMER USING THE SAME 有权
标题翻译：丙酰辅酶A转移酶突变体从丙酸梭菌及其制备方法PLA或PLA共聚物SO
公开(公告)号：EP2188375B1
公开(公告)日：2013-09-25
申请号：EP08792894.1
申请日：2008-07-25
申请人： LG Chem, Ltd.
发明人： PARK, Si Jae , YANG, Taek Ho , KANG, Hye Ok , LEE, Sang Hyun , LEE, Eun Jung , KIM, Tae Wan
IPC分类号： C12N15/54 , C12N9/10
CPC分类号： C12P7/625 , C12N9/1029 , C12N9/1048 , C12N9/13 , C12Y203/01 , C12Y208/03001 , Y02P20/52
摘要： Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E.coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E.coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.

10. 发明公开

EP2395075A2 RECOMBINANT RALSTONIA EUTROPHA CAPABLE OF PRODUCING POLYLACTIC ACID OR POLYLATIC ACID COPOLYMER, AND METHOD FOR PRODUCING POLYLACTIC ACID OR POLYLATIC ACID COPOLYMER USING SAME 有权
标题翻译：聚乳酸或聚乳酸共聚物，现代制造重组富养产碱及其制造方法或聚乳酸聚乳酸共聚物SO
公开(公告)号：EP2395075A2
公开(公告)日：2011-12-14
申请号：EP10738720.1
申请日：2010-02-03
申请人： LG Chem, Ltd.
发明人： YANG, Taek Ho , PARK, Si Jae , LEE, Eun Jeong , KANG, Hye Ok , KIM, Tae Wan , LEE, Sang Hyun
IPC分类号： C12N1/21 , C12N15/31 , C12N15/63
CPC分类号： C12P7/56 , C12N9/0006 , C12N9/1029 , C12N9/13 , C12N15/74 , C12P7/62 , C12P7/625 , C12Y101/01036 , C12Y203/01 , C12Y208/03001
摘要： Provided are a recombinant Ralstonia eutropha capable of producing polylactate or a hydroxyalkanoate-lactate copolymer, and a method of preparing polylactate or a hydroxyalkanoate-lactate copolymer using the same. The recombinant Ralstonia eutropha, which is prepared by introducing a gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate thereto, may be cultured, thereby efficiently preparing a lactate polymer and a lactate copolymer.
摘要翻译：本发明提供一种重组富养产碱能够产生聚乳酸酯或羟基链烷酸酯 - 乳酸酯共聚物的，和制备聚乳酸酯或使用其的羟基链烷酸酯 - 乳酸酯共聚物的方法。这是通过引入酶转化乳酸成乳酰CoA的基因和聚羟基脂肪酸酯的基因制备的重组富养产碱，全部采用（PHA）合酶乳酰基-CoA作为底物于此，可以是培养的，从而有效地制备乳酸聚合物和乳酸酯共聚物。

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