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    • 2. 发明申请
    • SYSTEMS AND METHODS FOR FEEDSTOCK QUALITY ASSESSMENT
    • 用于饲料质量评估的系统和方法
    • WO2012078885A3
    • 2013-01-24
    • PCT/US2011063963
    • 2011-12-08
    • POET RES INCLEWIS STEPHEN MNARENDRANATH NEELAKANTAM V
    • LEWIS STEPHEN MNARENDRANATH NEELAKANTAM V
    • C12Q1/44G01N33/10
    • C12Q1/44G01N33/10G01N2333/415
    • Assessing quality of feedstock is provided and may be useful for determining quality of feed (such as corn kernels). The assessment determines endogenous enzyme activity within the feedstock, which correlates with total ethanol yields in raw starch hydrolysis (non-cooked) systems. In some embodiments, a sample containing flour (ground feedstock) is provided. In some cases, flour is diluted in water and buffered with a phosphate buffered solution. The buffered flour solution is contacted with a molecule, or fluorescent dye, such as fluorescein diacetate and/or difluorofluorescein that is altered by enzymatic (esterase) activity in a detectable fashion. Enzyme activity may be facilitated using an incubator. The detectible alteration may be measured using a fluorometer. In some embodiments, two incubations and fluorescence measurements can be performed. By subtracting the difference between the measured fluorescence, a fluorescence index may be found, which correlates with the endogenous enzyme activity in the feedstock sample.
    • 提供原料质量评估,可用于确定饲料质量(如玉米粒)。 该评估确定原料内的内源酶活性,其与生淀粉水解(未煮熟)体系中的总乙醇产量相关。 在一些实施方案中,提供含有面粉(研磨原料)的样品。 在某些情况下,将面粉稀释在水中并用磷酸盐缓冲溶液缓冲。 缓冲的面粉溶液与分子或荧光染料接触,例如以可检测的方式通过酶(酯酶)活性改变的荧光素二乙酸酯和/或二氟荧光素。 使用培养箱可以促进酶活性。 可以用荧光计测量可检测的变化。 在一些实施方案中,可以进行两次孵育和荧光测量。 通过减去测量的荧光之间的差异,可以发现与原料样品中的内源性酶活性相关的荧光指数。