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    • 4. 发明申请
    • SYSTEMS AND METHODS FOR FEEDSTOCK QUALITY ASSESSMENT
    • 用于饲料质量评估的系统和方法
    • WO2012078885A3
    • 2013-01-24
    • PCT/US2011063963
    • 2011-12-08
    • POET RES INCLEWIS STEPHEN MNARENDRANATH NEELAKANTAM V
    • LEWIS STEPHEN MNARENDRANATH NEELAKANTAM V
    • C12Q1/44G01N33/10
    • C12Q1/44G01N33/10G01N2333/415
    • Assessing quality of feedstock is provided and may be useful for determining quality of feed (such as corn kernels). The assessment determines endogenous enzyme activity within the feedstock, which correlates with total ethanol yields in raw starch hydrolysis (non-cooked) systems. In some embodiments, a sample containing flour (ground feedstock) is provided. In some cases, flour is diluted in water and buffered with a phosphate buffered solution. The buffered flour solution is contacted with a molecule, or fluorescent dye, such as fluorescein diacetate and/or difluorofluorescein that is altered by enzymatic (esterase) activity in a detectable fashion. Enzyme activity may be facilitated using an incubator. The detectible alteration may be measured using a fluorometer. In some embodiments, two incubations and fluorescence measurements can be performed. By subtracting the difference between the measured fluorescence, a fluorescence index may be found, which correlates with the endogenous enzyme activity in the feedstock sample.
    • 提供原料质量评估,可用于确定饲料质量(如玉米粒)。 该评估确定原料内的内源酶活性,其与生淀粉水解(未煮熟)体系中的总乙醇产量相关。 在一些实施方案中,提供含有面粉(研磨原料)的样品。 在某些情况下,将面粉稀释在水中并用磷酸盐缓冲溶液缓冲。 缓冲的面粉溶液与分子或荧光染料接触,例如以可检测的方式通过酶(酯酶)活性改变的荧光素二乙酸酯和/或二氟荧光素。 使用培养箱可以促进酶活性。 可以用荧光计测量可检测的变化。 在一些实施方案中,可以进行两次孵育和荧光测量。 通过减去测量的荧光之间的差异,可以发现与原料样品中的内源性酶活性相关的荧光指数。
    • 10. 发明公开
    • SYSTEM FOR MANAGEMENT OF YEAST TO FACILITATE THE PRODUCTION OF ETHANOL
    • 系统ZUR VERWALTUNG VON HEFEFÜRERLEICHTERTE ETHANOLHERSTELLUNG
    • EP3004325A4
    • 2017-01-18
    • EP13885981
    • 2013-05-28
    • POET RES INC
    • NARENDRANATH NEELAKANTAM VCARLSON DAVID CHARLES
    • C12N1/18C12M1/00C12N1/16C12P7/10
    • C12N1/18C12M21/12C12M43/02C12P7/10Y02E50/16
    • A system and method for managing an ethanologen for use in biorefinery is disclosed. The method for propagating ethanologen for use in the production of a fermentation product from biomass comprises the steps of providing a medium for propagation of ethanologen; supplying a first cell mass of ethanologen to the medium; supplying xylose to the medium as a carbon source for the ethanologen; and maintaining the medium comprising the first cell mass of ethanologen at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and About 37 degrees Celsius so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen. The second cell mass of ethanologen is larger than the first cell mass of ethanologen. The system for propagating ethanologen for use in the production of a fermentation product from biomass comprises a first stage comprising a first vessel configured to maintain a medium comprising ethanologen; a second stage comprising a second vessel configured to maintain a medium supplied from the first stage; a source of xylose to be provided to the medium as a carbon source for ethanologen in the first stage; and a source of xylose to be provided to the medium as a carbon source for the ethanologen in the second stage. The ethanologen has a first cell mass when supplied to the first stage and the ethanologen has a second cell mass when supplied from the first stage to the second stage and the ethanologen has a third cell mass when supplied from the second stage. The second cell mass is at least 200 times larger than the first cell mass The third cell mass is at least 20 times larger than the second cell mass.
    • 公开了一种用于生物炼制中用于管理种子学的系统和方法。 用于从生物质生产发酵产物中用于繁殖的酿酒酵母的方法包括以下步骤:提供用于繁殖的培养基; 向培养基中提供第一个细胞团的乙醇提取物; 向培养基中提供木糖作为乙醇的碳源; 并且将包含乙醇沸石的第一细胞团的培养基保持在约5.0至6.0之间的温度和约26至约37摄氏度之间的温度下,使得第一个细胞团的乙醇培养基传播到第二个细胞团 。 酿酒酵母的第二个细胞团大于第一批细胞团。 用于从生物质生产发酵产物的用于繁殖乙醇生物的系统包括第一阶段,其包括构造成维持包含乙醇培养基的培养基的第一容器; 第二级包括构造成保持从第一级供应的介质的第二容器; 在第一阶段提供作为乙醇生物碳源的培养基中的木糖来源; 和作为第二阶段的乙醇生物碳源的培养基提供的木糖来源。 当从第一阶段供应时,乙醇提取物具有第一细胞团,并且当从第一阶段供应时,乙醇生物具有第二细胞团,并且当从第二阶段供应时,所述乙醇培养物具有第三细胞团。 第二细胞质量比第一细胞质量至少大200倍。第三细胞质量比第二细胞团质量至少大20倍。