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1. 发明申请

WO2008008709A2 METHOD OF SELECTIVE PROTEIN ENRICHMENT AND ASSOCIATED APPLICATIONS 审中-公开
标题翻译：选择性蛋白质丰富和相关应用的方法
公开(公告)号：WO2008008709A2
公开(公告)日：2008-01-17
申请号：PCT/US2007072947
申请日：2007-07-06
申请人： LEAP BIOSCIENCE CORP , HUI CEN
发明人： HUI CEN
IPC分类号： G01N33/53
CPC分类号： G01N33/554 , G01N33/6803
摘要： Provided herein are methods of selective enrichment of ligands present in a suitable biological sample. According to the invention, one or a plurality of receptor carriers may be used to capture ligands which are capable of binding to receptors immobilized on the surface of the receptor carriers. The receptor carriers bound with the ligands are separated from the remaining sample and the ligands are then eluted with a ligand elution solution to result in a ligand-containing solution, which is further concentrated to give a ligand sample. In one embodiment, the receptor carriers are living cells comprising a plurality of receptors on the outer leaflet of cytoplasmic membranes. Ligand samples obtained by the present invention may be useful for ligand profiling, for example, via any known methods including 2-D gel electrophoresis coupled with mass spectrometry, for example.
摘要翻译：本文提供选择性富集存在于合适的生物样品中的配体的方法。根据本发明，可以使用一种或多种受体载体捕获能够结合固定在受体载体表面上的受体的配体。与配体结合的受体载体与剩余的样品分离，然后用配体洗脱溶液洗脱配体，得到含配体的溶液，其进一步浓缩得到配体样品。在一个实施方案中，受体载体是在细胞质膜外叶上包含多个受体的活细胞。通过本发明获得的配体样品可用于配体分析，例如通过任何已知的方法，包括与质谱联用的2-D凝胶电泳。

2. 发明申请

US20120115129A1 ENZYME SUBSTRATE COMPRISING A FUNCTIONAL DYE AND ASSOCIATED TECHNOLOGY AND METHODS 有权
标题翻译：包含功能性染色及其相关技术和方法的酶基质
公开(公告)号：US20120115129A1
公开(公告)日：2012-05-10
申请号：US13326009
申请日：2011-12-14
申请人： FEI MAO , HUI CEN , WAI-YEE LEUNG
发明人： FEI MAO , HUI CEN , WAI-YEE LEUNG
IPC分类号： C12Q1/37 , C12Q1/68 , C12Q1/25 , C07K17/02 , C07D413/06
CPC分类号： C12N9/96 , C07D263/60 , C07D417/06 , C07D501/00 , C07K7/06 , C12Q1/37 , C12Q1/48 , C12Q1/485 , G01N21/6486 , G01N33/582
摘要： Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.
摘要翻译：提供了本发明的酶底物和相关技术。本发明的酶底物可以包含生物功能性荧光染料和酶特异性底物部分，其功能性染料的官能度减弱。酶反应可以从酶底物切割底物部分的至少一部分以提供更多功能的产物染料。通常，在特定条件下，例如当结合到伴侣生物分子或伴侣生物分子的组合时，该产物染料通常可以是非荧光的或弱荧光的，并且是相对荧光的。因此，本发明的酶底物可用于荧光检测和/或各种有用应用中的任何一种，例如在无细胞系统或活细胞中检测酶活性，筛选药物，或疾病的诊断。

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