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    • 2. 发明授权
    • Method for in vitro phosphorylation of trap of Staphylococcus aureus and a method for screening the inhibitor of the trap phosphorylation
    • 金黄色葡萄球菌陷阱的体外磷酸化方法和筛选磷酸化抑制剂的方法
    • US07927829B2
    • 2011-04-19
    • US12185843
    • 2008-08-05
    • Kyeong Kyu KimSung Wook Kang
    • Kyeong Kyu KimSung Wook Kang
    • C12Q1/50
    • C07K14/31C12Q1/485G01N2500/04
    • The present invention relates to a method for in vitro phosphorylation of TRAP derived from Staphylococcus aureus. In the present invention, the TRAP is first identified as a kinase that self-phosphorylates and is phosphorylated specifically in the presence of an oxidative metal ion such as iron, copper and zinc. Based upon this finding, a novel method for in vitro phosphorylation of purified TRAP is provided and also, a method for screening various chemicals and natural materials by using the above method is provided in order to select inhibitors against the TRAP phosphorylation. The inhibitors are expected to be applied for novel antibiotics of Staphylococcus aureus, because this TRAP phosphorylation is essential to infect Staphylococcus aureus. Therefore, the present invention can be widely used to develop novel drugs against Staphylococcus aureus and their resistant strains, in near future.
    • 本发明涉及来自金黄色葡萄球菌的TRAP的体外磷酸化方法。 在本发明中,首先将TRAP鉴定为在氧化金属离子例如铁,铜和锌的存在下自磷酸化并被磷酸化的激酶。 基于这一发现,提供了一种用于纯化TRAP的体外磷酸化的新方法,并且还提供了通过使用上述方法筛选各种化学品和天然材料的方法,以选择抗TRAP磷酸化的抑制剂。 预期抑制剂应用于金黄色葡萄球菌的新型抗生素,因为这种TRAP磷酸化对感染金黄色葡萄球菌至关重要。 因此,本发明可以在不久的将来广泛用于开发抗金黄色葡萄球菌及其抗性菌株的新型药物。