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    • 9. 发明授权
    • Method for culturing animal cells
    • 培养动物细胞的方法
    • US5786215A
    • 1998-07-28
    • US436648
    • 1995-05-08
    • Peter C. BrownMark T. WiningerRobert V. Oakley
    • Peter C. BrownMark T. WiningerRobert V. Oakley
    • C12M3/06C12N5/00
    • C12M23/24C12M23/06C12M23/34
    • An apparatus and method for the in vitro culturing of animal cells, e.g., mammalian cells, to attain mass growth of the cells per se and/or to recover cell-secreted products of interest therefrom, in which an integral culture and gassing element is formed from spirally-wound or concentricallywrapped lengths of a liquid-impermeable, oxygen-permeable membrane envelope such that narrow cell culture spaces are formed between adjacent windings or wrapping of the envelope. Cells in the culture spaces, which spaces may optionally contain cell substrate material, are cultured in contact with a culture medium introduced into the spaces from an end of the element, and in contact with oxygen-containing gas supplied to the membrane envelope for diffusion across its oxygen-permeable walls into the culture spaces.
    • 用于体外培养动物细胞(例如哺乳动物细胞)的装置和方法,以实现细胞本身的大规模生长和/或回收其中形成整合培养物和放气元件的感兴趣的细胞分泌产物 从不透液体的透氧薄膜包膜的螺旋卷绕或同心包裹的长度,使得相邻绕组之间形成狭窄的细胞培养空间或包封。 将培养空间中的细胞任选地含有细胞底物材料的细胞与从元件末端引入到空间中的培养基接触培养,并与供应到膜包膜的含氧气体接触以扩散跨越 其透氧壁渗入培养空间。