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1. 发明授权

US06987008B1 Variant nucleoside-5′-phosphate producing enzyme 失效
标题翻译：变体核苷-5'-磷酸生成酶
公开(公告)号：US06987008B1
公开(公告)日：2006-01-17
申请号：US09807990
申请日：2000-09-01
申请人： Kohki Ishikawa , Ei-ichiro Suzuki , Keiko Gondoh , Nobuhisa Shimba , Yasuhiro Mihara , Hisashi Kawasaki , Osamu Kurahashi , Tohru Kouda , Megumi Shimaoka , Rie Kozutsumi , Yasuhisa Asano
发明人： Kohki Ishikawa , Ei-ichiro Suzuki , Keiko Gondoh , Nobuhisa Shimba , Yasuhiro Mihara , Hisashi Kawasaki , Osamu Kurahashi , Tohru Kouda , Megumi Shimaoka , Rie Kozutsumi , Yasuhisa Asano
IPC分类号： C12P19/30 , C12N9/14 , C07H21/04
CPC分类号： C12N9/12 , A61K38/00 , C12N9/16
摘要： There is provided a mutant nucleoside-5′-phosphate producing enzyme with improved nucleoside-5′-phosphate producing ability of a nucleoside-5′-phosphate producing enzyme that has transphosphorylation activity and/or phosphatase activity and has one Lys residue, two Arg residues and two His residues with distances between their Cα's within a particular ranges and a space around them allowing a binding of a nucleoside, of which mutation is designed by using the crystal structure of EB-AP.
摘要翻译：提供了具有转移磷酸化活性和/或磷酸酶活性并具有一个Lys残基的核苷-5'-磷酸生产酶的核苷-5'-磷酸生产能力的突变体核苷-5'-磷酸生产酶，两个Arg 残留物和两个His残基，其特定范围内的Calpha与其周围的空间之间的距离允许结合核苷，其中通过使用EB-AP的晶体结构设计突变。

2. 发明授权

US06207435B1 Method for producing nucleoside-5′-phosphate ester 有权
标题翻译：制备核苷-5'-磷酸酯的方法
公开(公告)号：US06207435B1
公开(公告)日：2001-03-27
申请号：US09417090
申请日：1999-10-13
申请人： Yasuhiro Mihara , Takashi Utagawa , Hideaki Yamada , Yasuhisa Asano
发明人： Yasuhiro Mihara , Takashi Utagawa , Hideaki Yamada , Yasuhisa Asano
IPC分类号： C12N916
CPC分类号： C12N9/16 , C12P19/30
摘要： A method for producing nucleoside-5′-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phospahte group donor using an acid phosphatase having an increased affinity for the nucleoside and/or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5′-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and/or an enhanced temperature stability are also provided.
摘要翻译：通过使用对核苷具有增加的亲和性的酸性磷酸酶和/或在pH3.0至pH3.0的pH升高的温度稳定性，磷酸化具有磷酸基供体的核苷的方法，以高产率生产核苷-5'-磷酸酯 5.5，生成核苷-5'-磷酸酯。还提供了对核苷具有增加的亲和力和/或提高的温度稳定性的突变型酸性磷酸酶。

3. 发明授权

US06015697A Method for producing nucleoside-5'-phosphate ester 失效
标题翻译：制备核苷-5'-磷酸酯的方法
公开(公告)号：US06015697A
公开(公告)日：2000-01-18
申请号：US975698
申请日：1997-11-21
申请人： Yasuhiro Mihara , Takashi Utagawa , Hideaki Yamada , Yasuhisa Asano
发明人： Yasuhiro Mihara , Takashi Utagawa , Hideaki Yamada , Yasuhisa Asano
IPC分类号： C12N15/09 , C12N1/21 , C12N9/16 , C12N15/00 , C12N15/55 , C12P19/30 , C12P19/36 , C12R1/01 , C12R1/185 , C12R1/19 , C12R1/22 , C12R1/425 , C12P19/38
CPC分类号： C12N9/16 , C12P19/30
摘要： A method for producing nucleoside-5'-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phospahte group donor using an acid phosphatase having an increased affinity for the nucleoside and/or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5'-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and/or an enhanced temperature stability are also provided.
摘要翻译：通过使用对核苷具有增加的亲和性的酸性磷酸酶和/或在pH3.0至pH3.0的pH升高的温度稳定性，磷酸化具有磷酸基供体的核苷的方法，以高产率生产核苷-5'-磷酸酯 5.5，生成核苷-5'-磷酸酯。还提供了对核苷具有增加的亲和力和/或提高的温度稳定性的突变型酸性磷酸酶。

4. 发明授权

US06355472B2 Method for producing nucleoside-5′-phosphate ester 有权
标题翻译：制备核苷-5'-磷酸酯的方法
公开(公告)号：US06355472B2
公开(公告)日：2002-03-12
申请号：US09727578
申请日：2000-12-04
申请人： Yasuhiro Mihara , Takashi Utagawa , Hideaki Yamada , Yasuhisa Asano
发明人： Yasuhiro Mihara , Takashi Utagawa , Hideaki Yamada , Yasuhisa Asano
IPC分类号： C12N120
CPC分类号： C12N9/16 , C12P19/30
摘要： A method for producing nucleoside-5′-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phosphate group donor using an acid phosphatase having an increased affinity for the nucleoside and/or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5′-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and/or an enhanced temperature stability are also provided.
摘要翻译：通过使用对核苷具有增加的亲和力的酸性磷酸酶和/或在pH3.0至pH3.0的pH升高的温度稳定性磷酸化磷酸基团供体的核苷而廉价且高收率地制备核苷-5'-磷酸酯的方法， 5.5，生成核苷-5'-磷酸酯。还提供了对核苷具有增加的亲和力和/或提高的温度稳定性的突变型酸性磷酸酶。

5. 发明授权

US6010851A Method for producing nucleoside-5'-phosphate ester 失效
标题翻译：制备核苷-5'-磷酸酯的方法
公开(公告)号：US6010851A
公开(公告)日：2000-01-04
申请号：US750145
申请日：1997-01-21
申请人： Yasuhiro Mihara , Takashi Utagawa , Hideaki Yamada , Yasuhisa Asano
发明人： Yasuhiro Mihara , Takashi Utagawa , Hideaki Yamada , Yasuhisa Asano
IPC分类号： C12N15/09 , C12N9/16 , C12N15/55 , C12P19/30 , C12P19/32 , C12R1/01 , C12R1/19 , C12Q1/68
CPC分类号： C12N9/16 , C12P19/32
摘要： Nucleoside-5'-phosphate ester is produced inexpensively and efficiently by allowing an acid phosphatase, especially an acid phosphatase having a lowered phosphomonoesterase activity to act under a condition of pH 3.0 to 5.5 on a nucleoside and a phosphate group donor selected from the group consisting of polyphosphoric acid or a salt thereof, phenylphosphoric acid or a salt thereof, and carbamyl phosphate or a salt thereof to produce nucleoside-5'-phosphate ester, and collecting it.
摘要翻译： PCT No.PCT / JP96 / 01402 Sec。 371日期1997年1月21日 102（e）1997年1月21日PCT PCT 1996年5月24日PCT公布。 WO96 / 37603 PCT出版物日期：1996年11月28日核苷-5'-磷酸酯是通过使酸性磷酸酶，特别是磷酸单酯酶活性降低的酸性磷酸酶在pH3.0〜5.5的条件下在核苷和磷酸基供体上起作用而廉价而有效地生产的选自多磷酸或其盐，苯基磷酸或其盐，磷酸氨基戊酯或其盐，以制备核苷-5'-磷酸酯，并将其收集。

6. 发明申请

US20170268033A1 Method of Analyzing L-Tryptophan in Biological Samples, and Kit Used Therein 审中-公开
公开(公告)号：US20170268033A1
公开(公告)日：2017-09-21
申请号：US15608460
申请日：2017-05-30
申请人： Yasuhisa Asano , Masafumi Kameya , Hiroyasu Onaka
发明人： Yasuhisa Asano , Masafumi Kameya , Hiroyasu Onaka
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26 , C12Y104/03
摘要： Disclosed is a method for quantifying L-tryptophan involving a step for mixing a specimen, L-tryptophan oxidase, and water, a step for allowing the obtained reaction solution to stand a predetermined period of time in the presence of oxygen, and a step for measuring the reaction product resulting from action of enzymes present in the reaction solution after allowing to stand. The L-tryptophan oxidase has a given amino acid sequence and has oxidase activity that generates hydrogen peroxide and ammonia by acting on the L-tryptophan in the presence of oxygen and water. The oxidase activity of the L-tryptophan oxidase on the L-phenylalanine is in the range of 0-3% of the oxidase activity thereof on the L-tryptophan, and the L-tryptophan oxidase does not have oxidase activity on protein-constituting amino acids other than L-tryptophan and L-phenylalanine. Also disclosed are a kit used to quantify the L-tryptophan containing L-tryptophan oxidase, and an enzyme sensor using the L-tryptophan oxidase. This method, kit and enzyme sensor use an L-tryptophan-specific enzyme, so are capable of quantifying L-tryptophan even in the presence of other amino acids.

7. 发明授权

US09181574B2 L-amino acid oxidase, method for measuring L-lysine, kit and enzyme sensor 有权
标题翻译： L-氨基酸氧化酶，测量L-赖氨酸，试剂盒和酶传感器的方法
公开(公告)号：US09181574B2
公开(公告)日：2015-11-10
申请号：US14445597
申请日：2014-07-29
申请人： Yasuhisa Asano , Daisuke Matsui
发明人： Yasuhisa Asano , Daisuke Matsui
IPC分类号： C12N9/06 , C12Q1/00 , C12Q1/26 , C12Q1/28
CPC分类号： C12Q1/005 , C12N9/0022 , C12Q1/26 , C12Q1/28 , C12Q2533/00
摘要： Methods are provided measuring L-lysine using a variant enzyme, an L-lysine measurement kit, and an enzyme sensor. Variant L-amino acid oxidase having a predetermined amino acid mutation, and having oxidase activity that is highly substrate-specific for L-lysine; a method for measuring L-lysine using this variant enzyme; an L-lysine measurement kit; and an enzyme sensor are also provided.
摘要翻译：提供使用变体酶，L-赖氨酸测量试剂盒和酶传感器测量L-赖氨酸的方法。具有预定氨基酸突变的具有对L-赖氨酸具有高度底物特异性的氧化酶活性的变体L-氨基酸氧化酶; 使用该变体酶测定L-赖氨酸的方法; L-赖氨酸测量试剂盒; 还提供酶传感器。

8. 发明授权

US07776570B2 L-amino acid amide asymmetric hydrolase and DNA encoding the same 有权
标题翻译： L-氨基酸酰胺不对称水解酶和DNA编码相同
公开(公告)号：US07776570B2
公开(公告)日：2010-08-17
申请号：US12174995
申请日：2008-07-17
申请人： Yasuhisa Asano , Atsushi Inoue
发明人： Yasuhisa Asano , Atsushi Inoue
IPC分类号： C12P13/04 , C12N9/78 , C12N9/80 , C07H21/04
CPC分类号： C12P13/04 , C12N9/80 , C12P13/12 , C12P41/006 , C12Y305/01004
摘要： A recombinant microorganism is produced by introducing a DNA encoding an enzyme which hydrolyzes an amido bond of L-amino acid amide, especially L-2-alkylcysteine amide, and L-amino acid is produced by using cells or cell processed product of the obtained microorganism.
摘要翻译：通过引入编码水解L-氨基酸酰胺的酰胺键的酶，特别是L-2-烷基半胱氨酸酰胺的DNA，通过使用得到的微生物的细胞或细胞加工产物产生L-氨基酸来制备重组微生物。

9. 发明授权

US06246741B1 Fuel assembly 有权
标题翻译：燃油组件
公开(公告)号：US06246741B1
公开(公告)日：2001-06-12
申请号：US09258443
申请日：1999-02-26
申请人： Kohichi Nunokawa , Yasuhisa Asano
发明人： Kohichi Nunokawa , Yasuhisa Asano
IPC分类号： G21C330
CPC分类号： G21C7/20 , Y02E30/32 , Y02E30/39
摘要： A fuel assembly for a pressurized water reactor having control rod guide thimbles (5) each having a dashpot (12) for protecting against flexural deformation which may impair insertability of a control rod The fuel assembly includes, a plurality of control rod guide thimbles (5) having bottom and top end portions fixedly secured to a lower nozzle (2) and an upper nozzle (4), respectively, disposed in opposition to each other. The dashpot (12) of each control rod guide thimble (5) includes a small diameter section (13b) having an outer diameter smaller than that of the control rod guide thimble (5) formed at an upper portion of the dashpot (12), and a large diameter section (13a) having an outer diameter substantially equal to that of the control rod guide thimble (5) formed at a lower portion of the dashpot (12). With the length of the control rod guide thimble (5) represented by L, the effective length (S) of the small diameter section (13b) is selected to lie within a range of from 0.03 L to 0.1 L.
摘要翻译：一种用于具有控制杆导向套管（5）的压力水反应器的燃料组件，每个具有缓冲器（12），用于防止可能损害控制棒的可插入性的弯曲变形。燃料组件包括多个控制杆导向套管（5 ），其底部和顶端部分分别固定地固定到彼此相对设置的下喷嘴（2）和上喷嘴（4）。每个控制杆导向套管（5）的缓冲器（12）包括一个小直径部分（13b），其外径小于形成在缓冲器（12）的上部处的控制杆导向套管（5）的外径，以及具有与形成在缓冲器（12）的下部的控制棒导向套管（5）的外径大致相等的外径的大直径部（13a）。以L表示的控制杆导向套管（5）的长度，小直径部分（13b）的有效长度（S）选择在0.03L至0.1L的范围内。

10. 发明授权

US4970157A Isolated phenylalanine dehydrogenase gene and process for production of phenylalanine dehydrogenase 失效
标题翻译：分离的苯丙氨酸脱氢酶基因和苯丙氨酸脱氢酶的生产方法
公开(公告)号：US4970157A
公开(公告)日：1990-11-13
申请号：US84238
申请日：1987-08-11
申请人： Yasuo Hibino , Yasuhisa Asano , Noriko Okazaki , Naganori Numao
发明人： Yasuo Hibino , Yasuhisa Asano , Noriko Okazaki , Naganori Numao
IPC分类号： C12N1/21 , C12N9/06 , C12N15/53 , C12P13/22
CPC分类号： C12N9/0018 , C12N9/0028 , C12P13/222
摘要： An isolated gene coding for phenylalanine dehydrogenase of a microorganism belonging to a genus selected from the group consisting of the genera Bacillus and Sporosarcina origin; plasmids containing the gene; microorganism transformed with the plasmid, a process for the production of phenylalanine dehydrogenase using the microogranism; and a process for the production of L-phenylalanine using the enzyme.
摘要翻译：编码属于选自芽孢杆菌属和孢子纲属的属的微生物的苯丙氨酸脱氢酶的分离基因; 含有该基因的质粒; 用质粒转化的微生物，使用微观色谱法生产苯丙氨酸脱氢酶的方法; 以及使用该酶生产L-苯丙氨酸的方法。

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