专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20140357524A1 Method for Quantifying Subject Substance 有权
标题翻译：量化物质的方法
公开(公告)号：US20140357524A1
公开(公告)日：2014-12-04
申请号：US14453189
申请日：2014-08-06
申请人： Yasuhisa Asano , Masafumi Kameya
发明人： Yasuhisa Asano , Masafumi Kameya
IPC分类号： C12Q1/48 , C12Q1/32 , C12Q1/34
CPC分类号： C12Q1/485 , C12Q1/25 , C12Q1/32 , C12Q1/34 , C12Q1/48 , G01N33/6812 , G01N33/6815
摘要： There is provided a method for quantifying a subject substance, of which typical examples are amino acids. The method of the present invention comprises the following steps: the step of allowing an enzyme that can generate pyrophosphate by using adenosine triphosphate (ATP) as a substrate with converting the subject substance to act on the subject substance to generate pyrophosphate; the step of allowing pyruvate pyrophosphate dikinase (PPDK) to act on the generated pyrophosphate in the presence of adenosine monophosphate (AMP) and phosphoenolpyruvate (PEP) to generate ATP, phosphoric acid, and pyruvate; and the step of quantifying the generated pyruvate, and amount of the subject substance is determined on the basis of the obtained amount of pyruvate. According to the present invention, an amino acid in a biological sample containing a lot of various kinds of contaminants such as inorganic phosphoric acid and urea can be conveniently and quickly quantified without being influenced by the contaminants.
摘要翻译：本发明提供了一种定量目标物质的方法，其中典型的实例是氨基酸。本发明的方法包括以下步骤：通过使用三磷酸腺苷（ATP）作为底物，使可以产生焦磷酸盐的酶转化为目标物质以产生焦磷酸盐; 在磷酸腺苷（AMP）和磷酸烯醇丙酮酸（PEP）存在下，使丙酮酸焦磷酸二激酶（PPDK）作用于产生的焦磷酸盐以产生ATP，磷酸和丙酮酸盐的步骤; 并且基于获得的丙酮酸的量来确定定量产生的丙酮酸的步骤和目标物质的量。根据本发明，可以方便快速地定量含有大量各种污染物如无机磷酸和尿素的生物样品中的氨基酸，而不受污染物的影响。

2. 发明授权

US08409841B2 Hydroxynitrile lyase 失效
公开(公告)号：US08409841B2
公开(公告)日：2013-04-02
申请号：US13210630
申请日：2011-08-16
申请人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
发明人： Yasuhisa Asano , Takanori Akiyama , Fujio Yu , Eiji Sato
IPC分类号： C12N9/00 , C12N9/88 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/88 , C12P7/42 , C12P13/004
摘要： An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

3. 发明申请

US20130052679A1 L-THREONINE ANALYSIS METHOD AND L-THREONINE DEHYDROGENASE 有权
公开(公告)号：US20130052679A1
公开(公告)日：2013-02-28
申请号：US13602473
申请日：2012-09-04
申请人： Yasuhisa Asano , Techawaree Ueatrongchit
发明人： Yasuhisa Asano , Techawaree Ueatrongchit
IPC分类号： C12Q1/32 , C12N15/63 , C12N1/21 , C12N15/53 , C12N1/19 , C12N1/15 , G01N21/75 , G01N21/64 , C12N9/04 , C12N5/10
CPC分类号： C12Q1/32 , C12N9/0006 , C12Q1/005
摘要： A method for analyzing L-threonine contained in an specimen, which includes the steps of mixing a sample containing the specimen with an L-threonine dehydrogenase derived from Cupriavidus necator and a coenzyme NAD+ and analyzing the amount of NADH or 2-amino-3-oxobutyric acid after a predetermined period; an L-threonine dehydrogenase derived from Cupriavidus necator, which is a novel L-threonine dehydrogenase (TDH; EC 1.1.1.103) and can be utilized in the above-mentioned analysis method; a method for preparing a gene or the like to be used in the preparation of the enzyme, or a method for preparing the enzyme; an L-threonine analysis kit which includes (A) the L-threonine dehydrogenase and (B) a coenzyme NAD+; an enzyme preparation for use in the analysis of L-threonine, which includes the L-threonine dehydrogenase contained in a buffer solution; and an enzyme sensor utilizing the L-threonine dehydrogenase.
摘要翻译：一种用于分析样品中所含的L-苏氨酸的方法，其包括以下步骤：将含有样品的样品与源自Cupriavidus Necator的L-苏氨酸脱氢酶和辅酶NAD +混合，并分析NADH或2-氨基-3- 预定时间后的氧代丁酸; 衍生自Cupriavidus Necator的L-苏氨酸脱氢酶，其是新型L-苏氨酸脱氢酶（TDH; EC 1.1.1.103），可用于上述分析方法; 制备用于制备酶的基因等的方法或其制备方法; 一种L-苏氨酸分析试剂盒，其包括（A）L-苏氨酸脱氢酶和（B）辅酶NAD +; 用于分析L-苏氨酸的酶制剂，其包括缓冲溶液中所含的L-苏氨酸脱氢酶; 和利用L-苏氨酸脱氢酶的酶传感器。

4. 发明申请

US20130005007A1 Method for Industrially Producing(s)-1,1,1-Trifluoro-2-Propanol 有权
标题翻译：工业生产-1,1,1-三氟-2-丙醇的方法
公开(公告)号：US20130005007A1
公开(公告)日：2013-01-03
申请号：US13519058
申请日：2011-02-14
申请人： Yasuhisa Asano , Kenichi Fuhshuku , Tetsuro Nishii , Akihiro Ishii
发明人： Yasuhisa Asano , Kenichi Fuhshuku , Tetsuro Nishii , Akihiro Ishii
IPC分类号： C12P7/04
CPC分类号： C12P7/04 , C12N1/16 , C12N15/81
摘要： Disclosed is a method for producing (S)-1,1,1-trifluoro-2-propanol with high optical purity and high yield by having at least one kind of microorganism, which is selected from the group consisting of Hansenula polymorpha, Pichia anomala, Candida parapsilosis, Candida mycoderma, Pichia naganishii, Candida saitoana, Cryptococcus curvatus, Saturnospora dispora, Saccharomyces bayanus and Pichia membranaefaciens, act on 1,1,1-trifluoroacetone. Since microorganisms found in nature are made to act in a natural state, the problems to be raised when a transformant or the like is used can be avoided in this method. Consequently, the method can be easily put in industrial practice.
摘要翻译：公开了具有高光学纯度和高产率的（S）-1,1,1-三氟-2-丙醇的方法，该方法具有至少一种微生物，其选自多形汉逊酵母（Hansenula polymorpha），异形毕赤酵母，奇异假丝酵母（Candida parapsilosis），真菌念珠菌（Candida mycoderma），日本毕赤酵母（Pichia naganishii），蚕豆假丝酵母（Candida saitoana），曲霉隐球菌（Cryptococcus curvatus），土星孢子（Saturnospora dispora），巴豆酵母（Saccharomyces bayanus）和毕赤酵母（Pichia membranaefaciens），作用于1,1,1-三氟丙酮。由于在自然界中发现的微生物使自然状态发生作用，所以在这种方法中可以避免使用转化体等引起的问题。因此，该方法可以轻松投入工业实践。

5. 发明授权

US06987008B1 Variant nucleoside-5′-phosphate producing enzyme 失效
标题翻译：变体核苷-5'-磷酸生成酶
公开(公告)号：US06987008B1
公开(公告)日：2006-01-17
申请号：US09807990
申请日：2000-09-01
申请人： Kohki Ishikawa , Ei-ichiro Suzuki , Keiko Gondoh , Nobuhisa Shimba , Yasuhiro Mihara , Hisashi Kawasaki , Osamu Kurahashi , Tohru Kouda , Megumi Shimaoka , Rie Kozutsumi , Yasuhisa Asano
发明人： Kohki Ishikawa , Ei-ichiro Suzuki , Keiko Gondoh , Nobuhisa Shimba , Yasuhiro Mihara , Hisashi Kawasaki , Osamu Kurahashi , Tohru Kouda , Megumi Shimaoka , Rie Kozutsumi , Yasuhisa Asano
IPC分类号： C12P19/30 , C12N9/14 , C07H21/04
CPC分类号： C12N9/12 , A61K38/00 , C12N9/16
摘要： There is provided a mutant nucleoside-5′-phosphate producing enzyme with improved nucleoside-5′-phosphate producing ability of a nucleoside-5′-phosphate producing enzyme that has transphosphorylation activity and/or phosphatase activity and has one Lys residue, two Arg residues and two His residues with distances between their Cα's within a particular ranges and a space around them allowing a binding of a nucleoside, of which mutation is designed by using the crystal structure of EB-AP.
摘要翻译：提供了具有转移磷酸化活性和/或磷酸酶活性并具有一个Lys残基的核苷-5'-磷酸生产酶的核苷-5'-磷酸生产能力的突变体核苷-5'-磷酸生产酶，两个Arg 残留物和两个His残基，其特定范围内的Calpha与其周围的空间之间的距离允许结合核苷，其中通过使用EB-AP的晶体结构设计突变。

6. 发明申请

US20170268033A1 Method of Analyzing L-Tryptophan in Biological Samples, and Kit Used Therein 审中-公开
公开(公告)号：US20170268033A1
公开(公告)日：2017-09-21
申请号：US15608460
申请日：2017-05-30
申请人： Yasuhisa Asano , Masafumi Kameya , Hiroyasu Onaka
发明人： Yasuhisa Asano , Masafumi Kameya , Hiroyasu Onaka
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26 , C12Y104/03
摘要： Disclosed is a method for quantifying L-tryptophan involving a step for mixing a specimen, L-tryptophan oxidase, and water, a step for allowing the obtained reaction solution to stand a predetermined period of time in the presence of oxygen, and a step for measuring the reaction product resulting from action of enzymes present in the reaction solution after allowing to stand. The L-tryptophan oxidase has a given amino acid sequence and has oxidase activity that generates hydrogen peroxide and ammonia by acting on the L-tryptophan in the presence of oxygen and water. The oxidase activity of the L-tryptophan oxidase on the L-phenylalanine is in the range of 0-3% of the oxidase activity thereof on the L-tryptophan, and the L-tryptophan oxidase does not have oxidase activity on protein-constituting amino acids other than L-tryptophan and L-phenylalanine. Also disclosed are a kit used to quantify the L-tryptophan containing L-tryptophan oxidase, and an enzyme sensor using the L-tryptophan oxidase. This method, kit and enzyme sensor use an L-tryptophan-specific enzyme, so are capable of quantifying L-tryptophan even in the presence of other amino acids.

7. 发明授权

US09181574B2 L-amino acid oxidase, method for measuring L-lysine, kit and enzyme sensor 有权
标题翻译： L-氨基酸氧化酶，测量L-赖氨酸，试剂盒和酶传感器的方法
公开(公告)号：US09181574B2
公开(公告)日：2015-11-10
申请号：US14445597
申请日：2014-07-29
申请人： Yasuhisa Asano , Daisuke Matsui
发明人： Yasuhisa Asano , Daisuke Matsui
IPC分类号： C12N9/06 , C12Q1/00 , C12Q1/26 , C12Q1/28
CPC分类号： C12Q1/005 , C12N9/0022 , C12Q1/26 , C12Q1/28 , C12Q2533/00
摘要： Methods are provided measuring L-lysine using a variant enzyme, an L-lysine measurement kit, and an enzyme sensor. Variant L-amino acid oxidase having a predetermined amino acid mutation, and having oxidase activity that is highly substrate-specific for L-lysine; a method for measuring L-lysine using this variant enzyme; an L-lysine measurement kit; and an enzyme sensor are also provided.
摘要翻译：提供使用变体酶，L-赖氨酸测量试剂盒和酶传感器测量L-赖氨酸的方法。具有预定氨基酸突变的具有对L-赖氨酸具有高度底物特异性的氧化酶活性的变体L-氨基酸氧化酶; 使用该变体酶测定L-赖氨酸的方法; L-赖氨酸测量试剂盒; 还提供酶传感器。

8. 发明授权

US07776570B2 L-amino acid amide asymmetric hydrolase and DNA encoding the same 有权
标题翻译： L-氨基酸酰胺不对称水解酶和DNA编码相同
公开(公告)号：US07776570B2
公开(公告)日：2010-08-17
申请号：US12174995
申请日：2008-07-17
申请人： Yasuhisa Asano , Atsushi Inoue
发明人： Yasuhisa Asano , Atsushi Inoue
IPC分类号： C12P13/04 , C12N9/78 , C12N9/80 , C07H21/04
CPC分类号： C12P13/04 , C12N9/80 , C12P13/12 , C12P41/006 , C12Y305/01004
摘要： A recombinant microorganism is produced by introducing a DNA encoding an enzyme which hydrolyzes an amido bond of L-amino acid amide, especially L-2-alkylcysteine amide, and L-amino acid is produced by using cells or cell processed product of the obtained microorganism.
摘要翻译：通过引入编码水解L-氨基酸酰胺的酰胺键的酶，特别是L-2-烷基半胱氨酸酰胺的DNA，通过使用得到的微生物的细胞或细胞加工产物产生L-氨基酸来制备重组微生物。

9. 发明授权

US06246741B1 Fuel assembly 有权
标题翻译：燃油组件
公开(公告)号：US06246741B1
公开(公告)日：2001-06-12
申请号：US09258443
申请日：1999-02-26
申请人： Kohichi Nunokawa , Yasuhisa Asano
发明人： Kohichi Nunokawa , Yasuhisa Asano
IPC分类号： G21C330
CPC分类号： G21C7/20 , Y02E30/32 , Y02E30/39
摘要： A fuel assembly for a pressurized water reactor having control rod guide thimbles (5) each having a dashpot (12) for protecting against flexural deformation which may impair insertability of a control rod The fuel assembly includes, a plurality of control rod guide thimbles (5) having bottom and top end portions fixedly secured to a lower nozzle (2) and an upper nozzle (4), respectively, disposed in opposition to each other. The dashpot (12) of each control rod guide thimble (5) includes a small diameter section (13b) having an outer diameter smaller than that of the control rod guide thimble (5) formed at an upper portion of the dashpot (12), and a large diameter section (13a) having an outer diameter substantially equal to that of the control rod guide thimble (5) formed at a lower portion of the dashpot (12). With the length of the control rod guide thimble (5) represented by L, the effective length (S) of the small diameter section (13b) is selected to lie within a range of from 0.03 L to 0.1 L.
摘要翻译：一种用于具有控制杆导向套管（5）的压力水反应器的燃料组件，每个具有缓冲器（12），用于防止可能损害控制棒的可插入性的弯曲变形。燃料组件包括多个控制杆导向套管（5 ），其底部和顶端部分分别固定地固定到彼此相对设置的下喷嘴（2）和上喷嘴（4）。每个控制杆导向套管（5）的缓冲器（12）包括一个小直径部分（13b），其外径小于形成在缓冲器（12）的上部处的控制杆导向套管（5）的外径，以及具有与形成在缓冲器（12）的下部的控制棒导向套管（5）的外径大致相等的外径的大直径部（13a）。以L表示的控制杆导向套管（5）的长度，小直径部分（13b）的有效长度（S）选择在0.03L至0.1L的范围内。

10. 发明授权

US4970157A Isolated phenylalanine dehydrogenase gene and process for production of phenylalanine dehydrogenase 失效
标题翻译：分离的苯丙氨酸脱氢酶基因和苯丙氨酸脱氢酶的生产方法
公开(公告)号：US4970157A
公开(公告)日：1990-11-13
申请号：US84238
申请日：1987-08-11
申请人： Yasuo Hibino , Yasuhisa Asano , Noriko Okazaki , Naganori Numao
发明人： Yasuo Hibino , Yasuhisa Asano , Noriko Okazaki , Naganori Numao
IPC分类号： C12N1/21 , C12N9/06 , C12N15/53 , C12P13/22
CPC分类号： C12N9/0018 , C12N9/0028 , C12P13/222
摘要： An isolated gene coding for phenylalanine dehydrogenase of a microorganism belonging to a genus selected from the group consisting of the genera Bacillus and Sporosarcina origin; plasmids containing the gene; microorganism transformed with the plasmid, a process for the production of phenylalanine dehydrogenase using the microogranism; and a process for the production of L-phenylalanine using the enzyme.
摘要翻译：编码属于选自芽孢杆菌属和孢子纲属的属的微生物的苯丙氨酸脱氢酶的分离基因; 含有该基因的质粒; 用质粒转化的微生物，使用微观色谱法生产苯丙氨酸脱氢酶的方法; 以及使用该酶生产L-苯丙氨酸的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式