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1. 发明授权

US4234682A Method and reagent for determining biologically active heparin in plasma 失效
标题翻译：用于测定血浆中生物活性肝素的方法和试剂
公开(公告)号：US4234682A
公开(公告)日：1980-11-18
申请号：US13759
申请日：1979-02-21
申请人： Knut Bartl , Helmut Lill , Joachim Ziegenhorn
发明人： Knut Bartl , Helmut Lill , Joachim Ziegenhorn
IPC分类号： G01N33/66 , C12Q1/56 , G01N33/86 , C12Q1/38
CPC分类号： C12Q1/56 , C12Q2337/12 , G01N2333/8117 , G01N2333/96444 , G01N2333/974 , G01N2400/40 , Y10T436/108331 , Y10T436/143333
摘要： A method for determining biologically active heparin comprising adding, in the absence of exogenous antithrombin III, a proteolytic enzyme selected from the group consisting of thrombin and factor Xa and a chromogen substrate for the enzyme to a heparin sample, measuring the dye released from the chromogen substrate and thus determining the amount of heparin present.
摘要翻译：一种用于测定生物活性肝素的方法，包括在不存在外源性抗凝血酶III的情况下，将选自凝血酶和因子Xa的蛋白水解酶和所述酶的色原底物加入到肝素样品中，测量从色原体释放的染料底物，从而确定存在的肝素量。

2. 发明授权

US4409327A Process and reagent for the determination of the biological activity of heparin in plasma 失效
标题翻译：用于测定血浆中肝素生物活性的方法和试剂
公开(公告)号：US4409327A
公开(公告)日：1983-10-11
申请号：US233536
申请日：1981-02-11
申请人： Knut Bartl , Helmut Lill , Peter Roeschlau , Joachim Ziegenhorn
发明人： Knut Bartl , Helmut Lill , Peter Roeschlau , Joachim Ziegenhorn
IPC分类号： G01N33/66 , C12Q1/37 , C12Q1/56 , G01N33/50 , G01N33/86 , C12Q1/38
CPC分类号： G01N33/86 , C12Q2337/12 , G01N2333/8117 , G01N2333/96444 , G01N2333/974 , G01N2400/40 , Y10S435/81
摘要： Method and reagent for the determination of the biological activity of heparin in plasma by adding thrombin or Factor X.sub.a as the proteolytic enzyme and a chromogen substrate of the latter and measuring the dye liberated from the chromogen substrate in the absence of exogenous anti-thrombin III, the improvement consisting of adding, additionally, at least one compound of the formula ##STR1## wherein X is oxygen or the group NR.sub.5 ;and R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are independently selected from hydrogen or alkyl of 1 to 2 carbon atoms.
摘要翻译：通过添加凝血酶或因子Xa作为蛋白水解酶和后者的色底物，测定血浆中肝素生物活性的方法和试剂，并测定在没有外源性抗凝血酶III条件下从色底物释放的染料，该改进包括另外加入至少一种式（IMAGE）化合物，其中X是氧或NR5基团; 并且R 1，R 2，R 3，R 4和R 5独立地选自氢或1至2个碳原子的烷基。

3. 发明授权

US4301028A Control reagent for heparin activity determination 失效
标题翻译：用于肝素活性测定的对照试剂
公开(公告)号：US4301028A
公开(公告)日：1981-11-17
申请号：US110581
申请日：1980-01-09
申请人： Knut Bartl , Joachim Ziegenhorn , Peter Wunderwald , Klaus Beaucamp , Helmut Lill
发明人： Knut Bartl , Joachim Ziegenhorn , Peter Wunderwald , Klaus Beaucamp , Helmut Lill
IPC分类号： G01N33/66 , G01N33/50 , G01N33/86 , G01N33/96 , G01N33/16 , C09K3/00
CPC分类号： G01N33/96 , G01N2400/40 , G01N33/86 , Y10T436/10 , Y10T436/106664
摘要： A control reagent for heparin activity determination comprising20 to 1500 USP heparin,2000 to 30000 IU antithrombin III,0.15 to 1.5 millimoles serum albumin,0.005 to 0.05 mole ethylenediaminetetraacetic acid sodium salt,0 to 0.4 mole saccharose,2000 to 20000 U aprotinin, andbuffer substance, pH 6.5 to 7.5,per liter of control reagent solution.
摘要翻译：用于肝素活性测定的对照试剂，其包含20至1500USB肝素，2000至30000IU抗凝血酶III，0.15至1.5毫摩尔血清白蛋白，0.005至0.05摩尔乙二胺四乙酸钠盐，0至0.4摩尔蔗糖，2000至20000U抑肽酶，和缓冲物质，pH 6.5〜7.5，每升对照试剂溶液。

4. 发明授权

US4486531A Method and reagent for the determination of .beta.-lipoprotein 失效
标题翻译：用于测定β-脂蛋白的方法和试剂
公开(公告)号：US4486531A
公开(公告)日：1984-12-04
申请号：US489139
申请日：1983-05-04
申请人： Joachim Ziegenhorn , Knut Bartl , Max Brandhuber
发明人： Joachim Ziegenhorn , Knut Bartl , Max Brandhuber
IPC分类号： G01N33/68 , C12Q1/44 , G01N33/92 , C12Q1/60
CPC分类号： C12Q1/44 , G01N33/92 , G01N2800/044 , Y10S435/81 , Y10T436/104165 , Y10T436/105831 , Y10T436/107497 , Y10T436/25125 , Y10T436/25375
摘要： The present invention provides a process for the direct turbidimetric determination of .beta.-lipoproteins (LDL) in body fluids by precipitation with polyanions and divalent cations, wherein the precipitation is carried out in the presence of a complex former at a pH value of from 7 to 9.The present invention also provides a reagent for the direct turbidimetric determination of .beta.-lipoproteins (LDL) in body fluids, comprising a polyanion, a salt of a divalent metal, a complex former and a substance buffering at pH 7 to 9.
摘要翻译：本发明提供一种通过用聚阴离子和二价阳离子沉淀直接比浊测定体液中的β-脂蛋白（LDL）的方法，其中沉淀是在复合物的存在下在pH值为7至本发明还提供用于直接比浊测定体液中的β-脂蛋白（LDL）的试剂，其包含聚阴离子，二价金属的盐，复合物和在pH7至9下缓冲的物质。

5. 发明授权

US4409328A Method and reagent for the determination of glycerol 失效
标题翻译：测定甘油的方法和试剂
公开(公告)号：US4409328A
公开(公告)日：1983-10-11
申请号：US302861
申请日：1981-09-16
申请人： Joachim Ziegenhorn , Knut Bartl , Albert Roder , Gunter Lang , Hans Mollering , Ulrich Nagele
发明人： Joachim Ziegenhorn , Knut Bartl , Albert Roder , Gunter Lang , Hans Mollering , Ulrich Nagele
IPC分类号： G01N33/50 , C12N9/04 , C12Q1/26 , C12Q1/44 , C12Q1/61 , C12Q1/28 , C12Q1/30 , C12Q1/32
CPC分类号： C12Q1/61 , C12Q1/26 , Y10S435/805 , Y10S435/81
摘要： For the determination of glycerol, the latter is incubated with galactose oxidase in an aqueous medium in the presence of oxygen and either the oxygen consumption or the amount of H.sub.2 O.sub.2 or glyceraldehyde that is formed is determined. A reagent suitable for this purpose consists of galactose oxidase and a system for the determination of H.sub.2 O.sub.2 or a system for the determination of glyceraldehyde. It can additionally contain an agent for the saponification of esterified glycerol.
摘要翻译：为了测定甘油，将后者与半乳糖氧化酶在氧气存在下在水性介质中温育，并测定氧气消耗量或形成的H 2 O 2或甘油醛的量。适合于此目的的试剂由半乳糖氧化酶和用于测定H 2 O 2的系统或用于测定甘油醛的系统组成。它还可以含有酯化甘油皂化剂。

6. 发明授权

US4544630A Process for specific determination of cholesterol in the presence of the HDL fraction in serum 失效
$Process for specific determination of cholesterol in the presence of the HDL fraction in serum$
标题翻译：在血清中HDL级分存在下特异性测定胆固醇的方法
公开(公告)号：US4544630A
公开(公告)日：1985-10-01
申请号：US468792
申请日：1983-02-22
申请人： Joachim Ziegenhorn , Albert Roder , Knut Bartl , Gunter Wehmeyer
发明人： Joachim Ziegenhorn , Albert Roder , Knut Bartl , Gunter Wehmeyer
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/44 , C12Q1/60
CPC分类号： C12Q1/60
摘要： The present invention provides a process for the specific determination of the cholesterol of the LDL fraction in the presence of the HDL fraction of the lipoproteins of serum by the action of cholesterol esterase for the liberation of the cholesterol and oxidation of the liberated cholesterol with cholesterol oxidase and oxygen with the formation of hydrogen peroxide and cholestenone and kinetic measurement of the change of one of the reaction components of the oxidase reaction, especially the formation of hydrogen peroxide, wherein the measurement is carried out in a predetermined period of time, the reaction solution having a tenside concentration of 0.01 to 1.5 mmol/liter, a cholesterol esterase concentration of 0.1 to 30 U/ml. and a pH value of 6.5 to 8.0.The present invention also provides a reagent for carrying out this process.
摘要翻译：本发明提供了一种在胆固醇酯酶的作用下，通过胆固醇释放和胆固醇氧化酶氧化释放的胆固醇，在血清脂蛋白的HDL部分存在下，特异性测定LDL级分的胆固醇的方法和氧气，形成过氧化氢和胆甾烯酮，动力学测量氧化酶反应的一种反应成分的变化，特别是过氧化氢的形成，其中测量在预定时间内进行，反应溶液其表面活性剂浓度为0.01〜1.5mmol / l，胆固醇酯酶浓度为0.1〜30U / ml。 pH值为6.5〜8.0。本发明还提供了一种用于进行该方法的试剂。

7. 发明授权

US5288606A Reagent for specific determination of fructosamine 失效
标题翻译：具体测定果糖胺的试剂
公开(公告)号：US5288606A
公开(公告)日：1994-02-22
申请号：US911282
申请日：1992-07-09
申请人： Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
发明人： Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/30 , C12Q1/44 , C12Q1/54 , G01N33/66 , C12Q1/00 , A61K35/78 , A61K37/02 , C12P19/38
CPC分类号： C12Q1/26 , G01N33/66 , Y10S436/825 , Y10S436/904 , Y10T436/144444 , Y10T436/25125
摘要： The present invention provides a process for the specific determination of the serum fructosamine content in blood or samples derived from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, wherein, before the color reaction, non-specific reducing-acting and/or turbidity-causing sample components are removed at approximately neutral pH value, subsequently the pH is adjusted to a value of from 10 to 12 and the color reagent is added thereto.The present invention also provides a reagent mixture for the specific determination of the serum fructosamine content in blood or samples derived from blood, wherein it comprises a reagent for the removal of non-specific reducing-acting and/or turbidity-causing sample components, a rebuffering reagent with a buffer which has a pH value in the range of from 10.5 to 12.5 and a color reagent for the detection of fructosamine.
摘要翻译：本发明提供一种用于通过与适当的着色试剂反应并测量由此引起的颜色变化来特异性测定血液或血液中衍生血液的血清果糖胺含量的方法，其中在显色反应之前，非特异性还原在大约中性pH值下去除起泡和/或浊度的样品组分，随后将pH调节至10至12的值，并向其中加入着色剂。本发明还提供了用于特异性测定血液或血液中衍生血液中的果糖胺含量的试剂混合物，其中它包括用于除去非特异性还原作用和/或引起浊度的样品组分的试剂，具有pH值在10.5〜12.5范围内的缓冲液和用于检测果糖胺的显色试剂的缓冲试剂。

8. 发明授权

US5108913A Process for preparing a maltoheptaose derivative 失效
标题翻译：制备MALTOHEPTAOSE衍生物的方法
公开(公告)号：US5108913A
公开(公告)日：1992-04-28
申请号：US545092
申请日：1990-06-27
申请人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Michal Gerhard , Gunter Weimann
发明人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Michal Gerhard , Gunter Weimann
IPC分类号： G01N33/50 , C07H15/04 , C07H15/203 , C12Q1/40
CPC分类号： C12Q1/40 , C12Q2334/10 , Y10S435/805 , Y10S435/81
摘要： .alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided. A process for the preparation of a maltoheptaose will Bacillus macerans amylase, free of p-nitrophenyl-alpha-glucoside splitting activity is disclosed.

9. 发明授权

US4892815A Process and reagent for the specific determination of the cholesterol of the HDL fraction 失效
$Process and reagent for the specific determination of the cholesterol of the HDL fraction$
标题翻译：用于特异性测定HDL级分胆固醇的方法和试剂
公开(公告)号：US4892815A
公开(公告)日：1990-01-09
申请号：US107467
申请日：1987-10-06
申请人： Lorenz Kerscher , Brigitte Pautz , Gisela Trunk , Joachim Ziegenhorn
发明人： Lorenz Kerscher , Brigitte Pautz , Gisela Trunk , Joachim Ziegenhorn
IPC分类号： C12Q1/26 , C12Q1/44 , C12Q1/60 , G01N33/92
CPC分类号： C12Q1/60 , G01N33/92 , G01N2800/044 , Y10S435/805 , Y10S435/962 , Y10S436/81 , Y10S436/824
摘要： The present invention provides a process for the specific determination of the cholesterol of the HDL fraction in the presence of the LDL fraction of serum lipoproteins. Pancreatic cholesterol esterase is used to liberate cholesterol, and the liberated cholesterol then reacts with cholesterol oxidase and oxygen to form hydrogen peroxide. The kinetics of either of hydrogen peroxide formation or oxygen consumption is measured within 2 to 15 minutes after the start of the reaction between cholesterol and the oxidase. The temperature is maintained within a range of 20.degree. C., during a predetermined time interval. Specific concentrations of reactants are maintained in the reaction solution, i.e., from 0.05 to 30 U/ml pancreatic cholesterol esterase; from 0.1 to 50 U/ml cholesterol oxidase; from 1.0 to 20 mMole/liter of a tenside of the bile acid group, and 0.1 to 10 g/liter of a non-ionic detergent. The pH is kept within a range of 5 to 9. In addition, a reagent is provided which is used for specific determination of HDL fraction cholesterol in the presence of the LDL fraction of serum lipoproteins. The reagent contains pancreatic esterase (0.05 to 30 U/ml); cholesterol oxidase (0.1 to 50 U/ml) a tenside of the bile acid grop (1.5 to 8 mMole/liter) and a non-ionic detergent (0.1 to 10 g/liter), all concentrations referring to the dilution used in the test. The reagent also contains a buffer at a pH of from 5 to 9, and a system for photometric determination of hydrogen peroxide.
摘要翻译：本发明提供了在血清脂蛋白的LDL级分存在下具体测定HDL级分的胆固醇的方法。胰胆固醇酯酶用于释放胆固醇，然后释放的胆固醇与胆固醇氧化酶和氧气反应形成过氧化氢。在胆固醇和氧化酶反应开始后2至15分钟内测量过氧化氢形成或氧气消耗的动力学。在预定的时间间隔内将温度保持在20℃的范围内。反应物的特定浓度保持在反应溶液中，即0.05至30U / ml胰胆固醇酯酶; 0.1至50U / ml胆固醇氧化酶; 1.0至20mMole /升的胆汁酸基的表面活性剂和0.1至10g /升的非离子型洗涤剂。 pH值保持在5〜9的范围。此外，提供了在血清脂蛋白的LDL级分存在下用于特异性测定HDL级分胆固醇的试剂。试剂含有胰酯酶（0.05〜30U / ml）; 胆固醇氧化酶（0.1至50U / ml）胆汁酸组织（1.5至8mMole /升）的表面活性剂和非离子型洗涤剂（0.1至10g /升），所有浓度均指试验中使用的稀释液。该试剂还含有pH为5至9的缓冲液和用于光度测定过氧化氢的系统。

10. 发明授权

US4816393A Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof 失效
标题翻译：核苷三磷酸依赖性1-甲基乙内酰脲酶，其获得方法及其用途
公开(公告)号：US4816393A
公开(公告)日：1989-03-28
申请号：US704712
申请日：1985-02-25
申请人： Joachim Siedel , Rolf Deeg , Albert Roder , Joachim Ziegenhorn , Hans Mollering , Helmgard Gauhl
发明人： Joachim Siedel , Rolf Deeg , Albert Roder , Joachim Ziegenhorn , Hans Mollering , Helmgard Gauhl
IPC分类号： C12N9/14 , C12N9/86 , C12Q1/34 , C12R1/01 , C12R1/06 , C12R1/13 , C12R1/265 , G01N33/70 , C12Q1/26 , C12Q1/32
CPC分类号： C12N9/86 , C12Q1/34 , G01N33/70 , G01N2333/986 , Y10S435/81
摘要： The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions.The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it.Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination(a) of the hydrolysis product formed from 1-methylhydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or(b) of the simultaneously formed nucleoside diphosphate.
摘要翻译：本发明提供1-甲基乙内酰脲酶，其在核苷三磷酸和多价金属离子的存在下水解1-甲基乙内酰脲。本发明还提供了获得1-甲基乙内酰脲酶和含有它的试剂的方法。此外，本发明提供了通过将肌酐与肌酐去酰亚胺酶（EC 3.5.4.21）转化为1-甲基乙内酰脲来测定肌酸酐的方法，后者在核苷三磷酸存在下用1-甲基乙内酰脲酶水解，测定（a）由N-甲基乙内酰脲与N-氨基甲酰基肌氨酰氨基水解酶形成的水解产物（肌氨酸形成），并用肌氨酸氧化酶或肌氨酸脱氢酶检测肌氨酸，或（b）同时形成的二磷酸核苷。

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