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1. 发明授权

US5344613A Agitation stabilizer in solid-phase peptide synthesizer 失效
标题翻译：固相肽合成仪中的搅拌稳定剂
公开(公告)号：US5344613A
公开(公告)日：1994-09-06
申请号：US9080
申请日：1993-01-26
申请人： Kiyoshi Nokihara , Rintaro Yamamoto , Makoto Hazama , Shin Nakamura
发明人： Kiyoshi Nokihara , Rintaro Yamamoto , Makoto Hazama , Shin Nakamura
IPC分类号： B01J19/00 , B01L99/00 , C07K1/04 , C08F2/00 , C40B40/10 , C40B60/14 , C07K5/00
CPC分类号： B01L99/00 , B01J19/0046 , C07K1/045 , B01J2219/00286 , B01J2219/00288 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： In an automated solid-phase peptide synthesizing apparatus comprising at least one reaction vessel containing particulate resin as an insoluble support matrix which anchors elongating peptide chains yielded through coupling-assembly formation of peptide bonds, mixing agitation of reagents, as well as circulation agitation of washing solvents flushing the coupling-associated reaction solutions, introduced in peptide chain-assembly process steps into the reaction vessel, is effected by the bubbling of an inert gas forcibly passed through the reagents or washing solvent via a drainage port of the reaction vessel, in order to promote peptide synthesis. A removable agitation stabilizer is inserted into the reaction vessel as a barrier for inhibiting extra-vessel escape, due to action of the bubbling inert gas, of the reagents as well as the washing solvents, and of the support matrix anchoring the elongating peptide chains. The stabilizer also breaks clumping and coagulative skinning of elongated peptides bound to the support matrix, as effects further consequent of the action of the bubbling inert gas and levitated thereby toward the mouth of the reaction vessel during peptide synthesis. The stabilizer additionally acts as a baffle furthering mixing agitation, to promote dispersive mixing of the peptidyl resin with the reagents and washing solvents during peptide chain assembly; and It also serves to distribute washing solvents evenly along the inner surface of the reaction vessel.
摘要翻译：在一种自动化固相肽合成装置中，包括至少一个含有颗粒状树脂作为不溶性支持基质的反应容器，该反应容器通过肽键的偶联 - 组装形成固定延伸的肽链，混合搅拌试剂以及循环搅拌洗涤将通过肽链组装工艺步骤引入反应容器的偶联相关反应溶液冲洗到反应容器中，通过以反应容器的排水口强制通过试剂或洗涤溶剂的惰性气体起泡促进肽合成。将可移除的搅拌稳定剂插入到反应容器中，作为阻止由于起泡惰性气体的作用以及洗涤溶剂和固定伸长肽链的载体基质而导致的外部容器逸出的屏障。稳定剂还破坏结合到载体基质上的细长肽的结块和凝结性皮肤剥离，其作用是由于气泡惰性气体的作用而进一步引起，并且由此在肽合成期间悬浮于反应容器口中。稳定剂另外作为进一步混合搅拌的挡板，以促进肽链组装期间肽基树脂与试剂和洗涤溶剂的分散混合; 并且还用于沿着反应容器的内表面均匀分布洗涤溶剂。

2. 发明授权

US5395594A Simultaneous multiple chemical synthesizer 失效
标题翻译：同时多重化学合成仪
公开(公告)号：US5395594A
公开(公告)日：1995-03-07
申请号：US933729
申请日：1992-08-24
申请人： Kiyoshi Nokihara , Makoto Hazama , Rintaro Yamamoto , Shin Nakamura
发明人： Kiyoshi Nokihara , Makoto Hazama , Rintaro Yamamoto , Shin Nakamura
IPC分类号： B01J19/00 , B01J19/28 , C07K1/04 , C07K5/08 , C07K7/06 , C07K14/575 , C07K14/655 , C07K14/70 , C40B40/10 , C40B60/14 , B01J8/02
CPC分类号： B01J19/004 , B01J19/0046 , C07K1/045 , B01J2219/00286 , B01J2219/00288 , B01J2219/00308 , B01J2219/00373 , B01J2219/00423 , B01J2219/00493 , B01J2219/005 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： The present invention is directed to a simultaneous multiple chemical synthesizer comprising a number of reaction vessels wherein each vessel has a filter in the bottom portion thereof, a number of needles wherein each needle is connected to an aspiration injection line of a reaction mixture and a gas supply line in connection with each reaction vessel and each needle does not touch the resin contained in the reaction vessel, a number of arms which are horizontally and vertically movable and hold the respective needles, a bubbling gas line and a waste discharge line in connection with each reaction vessel, wherein each line is connected to the bottom portion of each reaction vessel, a number of purging means which move synchronously with said waste discharge lines, and means for washing the portions of the needles and purge means which contact with the reaction reagents.
摘要翻译：本发明涉及一种同时多重化学合成器，其包括多个反应容器，其中每个容器在其底部具有过滤器，多个针，其中每个针连接到反应混合物的抽吸注入管线和气体与每个反应容器相关的供应管线，并且每个针不接触包含在反应容器中的树脂，可水平和垂直移动并保持各针的多个臂，鼓泡气管线和排放废水排放管线与每个反应容器，其中每条管线连接到每个反应容器的底部，与所述废物排放管线同步移动的多个清洗装置，以及用于清洗针头的部分和与反应试剂接触的清洗装置的装置。

3. 发明授权

US5356596A Apparatus for isolation of synthetic peptide without mechanical loss 失效
标题翻译：无机械损失分离合成肽的装置
公开(公告)号：US5356596A
公开(公告)日：1994-10-18
申请号：US22036
申请日：1993-02-24
申请人： Kiyoshi Nokihara , Rintaro Yamamoto , Shin Nakamura
发明人： Kiyoshi Nokihara , Rintaro Yamamoto , Shin Nakamura
IPC分类号： B01J19/00 , C07K1/04 , C08F2/00 , C40B40/10 , C40B50/14 , C40B60/14 , B01L3/00 , C12M1/00
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00288 , B01J2219/00308 , B01J2219/00454 , B01J2219/005 , B01J2219/00725 , C40B40/10 , C40B50/14 , C40B60/14
摘要： In connection with an automated peptide synthesizer, an apparatus and associated method through which peptides produced in simultaneous multiple synthesis are together isolable. Cleavage to liberate the peptides from linkage to the support matrix of the solid phase is carried out in the same vessels in which the synthesizing reactions took place. Prior to cleavage, a drainage port of each reaction vessel is closed off by a cap. The reaction vessel is inserted into a centrifuge tube, which in turn is put into a rack supporting a number of tubes equal to the number of channels of the peptide synthesizing apparatus. After the peptides are cleaved and dissolved into cleaving solution added into each of the reaction vessels, the caps are removed from the drainage ports of each reaction vessel, and the vessel is returned into the centrifuge tube. A plastic jet-fitting attached to the nozzle tip of a blow unit pressure gun is inserted into and pressed into contact against the supply opening of the reaction vessel. Operating a trigger of the pressure gun releases pressurized inert gas into the reaction chamber. The peptide-dissolved cleaving solution is thereby passed through the drainage port, and is thus transferred in liquid phase into the centrifuge tube as filtrate. Associated with the blow unit pressure gun, a needle tube fitting is provided for localized desiccation of wetted peptide following precipitation and centrifuging.
摘要翻译：关于自动肽合成仪，同时多重合成中产生的肽一起可分离的装置和相关方法。在与发生合成反应的相同的容器中进行从固定相的支持基质的连接释放肽的切割。在切割之前，每个反应容器的排水口由盖封闭。将反应容器插入到离心管中，该离心管又被放入支架中，该支架中的多个管等于肽合成装置的通道数。在肽被切割并溶解到每个反应容器中的裂解溶液中之后，从每个反应容器的排出口移除盖子，并将容器返回到离心管中。将附接到吹塑单元压力枪的喷嘴尖端的塑料喷射管插入并压接到反应容器的供应开口。操作压力枪的触发器将加压的惰性气体释放到反应室中。因此，肽溶解的解离溶液通过排出口，因此以液相方式转移到作为滤液的离心管中。与吹气单元压力枪相关联，提供针管配件，用于沉淀和离心后的湿肽的局部干燥。

4. 发明申请

US20080087547A1 Electrophoresis apparatus 失效
标题翻译：电泳仪
公开(公告)号：US20080087547A1
公开(公告)日：2008-04-17
申请号：US11905539
申请日：2007-10-02
申请人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
发明人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
IPC分类号： G01N27/447 , G01N21/64
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： For automating the operation of an electrophoresis apparatus and improving the throughput, the present electrophoresis apparatus has two platens capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit for sending a loading medium under pressure, a loading medium charging nozzle mechanism having a pair of nozzles connected to the loading medium charging unit, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens, a stacker mechanism for storing sample plates, a loading buffer solution supplying mechanism, a loading buffer solution dispensing mechanism connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates.
摘要翻译：为了使电泳装置的操作自动化并提高通过量，本电泳装置具有能够控制放置在其上的电泳板的温度的两个压板，用于在压力下发送加载介质的装载介质充电单元，具有连接到装载介质充电单元的一对喷嘴，用于分配样品以对放置在压板上的电泳板的分配开口进行采样的移液器机构，用于存储样品板的堆垛机构，装载缓冲溶液供应机构，装载缓冲溶液连接到装载缓冲溶液供应机构的分配机构，用于允许放置在台板上的每个电泳板电泳分离的动力单元，以及用于光学检测通过电泳板的每个电泳流路移动的组分的检测器。

5. 发明授权

US07655125B2 Electrophoresis apparatus 失效
标题翻译：电泳仪
公开(公告)号：US07655125B2
公开(公告)日：2010-02-02
申请号：US11905539
申请日：2007-10-02
申请人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
发明人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
IPC分类号： G01N27/453
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： For automating the operation of an electrophoresis apparatus and improving the throughput, the present electrophoresis apparatus has two platens capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit for sending a loading medium under pressure, a loading medium charging nozzle mechanism having a pair of nozzles connected to the loading medium charging unit, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens, a stacker mechanism for storing sample plates, a loading buffer solution supplying mechanism, a loading buffer solution dispensing mechanism connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates.
摘要翻译：为了使电泳装置的操作自动化并提高通过量，本电泳装置具有能够控制放置在其上的电泳板的温度的两个压板，用于在压力下发送加载介质的装载介质充电单元，具有连接到装载介质充电单元的一对喷嘴，用于分配样品以对放置在压板上的电泳板的分配开口进行采样的移液器机构，用于存储样品板的堆垛机构，装载缓冲溶液供应机构，装载缓冲溶液连接到装载缓冲溶液供应机构的分配机构，用于允许放置在台板上的每个电泳板电泳分离的动力单元，以及用于光学检测通过电泳板的每个电泳流路移动的组分的检测器。

6. 发明授权

US5470703A Method for peptide C-terminal fragment sequence analysis and apparatus for collecting peptide fragment 失效
标题翻译：肽C-末端片段序列分析方法及收集肽片段的方法
公开(公告)号：US5470703A
公开(公告)日：1995-11-28
申请号：US136761
申请日：1993-10-15
申请人： Kiyoshi Nokihara , Rintaro Yamamoto
发明人： Kiyoshi Nokihara , Rintaro Yamamoto
IPC分类号： C07K1/12 , C12Q1/37 , G01N33/68 , C12Q1/00 , C12M1/40 , C12N11/00
CPC分类号： C12Q1/37 , C07K1/128 , G01N33/6821 , Y10S436/81 , Y10T436/255
摘要： The present invention relates to a method for peptide C-terminal fragment sequence analysis, in which the fragment collection is carried out on an allylamine group-derivatized polymer membrane or on allylamine group-derivatized glass fiber filter paper; the collected C-terminal fragment is immobilized thereon using a water-soluble carbodiimide etc.; and the obtained immobilized product is subjected directly to amino acid sequence analysis. The present invention also relates to an apparatus for collecting a peptide fragment. According to the method of the present invention, peptides which are rich in hydrophobic groups in their C-terminus and are therefore difficult to trap with polyvalent ion carriers, currently used in the gas-phase sequencer, can be completely analyzed up to their C-terminus. Also, amino acid sequence analysis can be made even when the amount of C-terminal fragments is very small. In addition, since collection and immobilization of fragments can be done in the same bottle, the risks of contamination and mechanical loss are very low.
摘要翻译：本发明涉及一种肽C-末端片段序列分析方法，其中片段收集是在烯丙基胺基衍生的聚合物膜上或在烯丙胺基衍生的玻璃纤维滤纸上进行的; 收集的C-末端片段使用水溶性碳二亚胺等固定在其上; 将获得的固定化产物直接进行氨基酸序列分析。本发明还涉及一种收集肽片段的装置。根据本发明的方法，可以完全分析其C-末端富含疏水性基团并且因此难以与当前用于气相测序仪中的多价离子载体捕获的肽，终点。此外，即使C末端片段的量非常小，也可以进行氨基酸序列分析。此外，由于碎片的收集和固定可以在同一瓶中进行，污染和机械损失的风险非常低。

7. 发明授权

US5431882A Apparatus for collecting peptide fragment 失效
标题翻译：用于收集肽片段的装置
公开(公告)号：US5431882A
公开(公告)日：1995-07-11
申请号：US154515
申请日：1993-11-19
申请人： Kiyoshi Nokihara , Rintaro Yamamoto , Yoshiyuki Togawa , Mitsuhiro Hashimoto , Naoki Morita
发明人： Kiyoshi Nokihara , Rintaro Yamamoto , Yoshiyuki Togawa , Mitsuhiro Hashimoto , Naoki Morita
IPC分类号： C07K1/14 , C07K1/12 , G01N33/68
CPC分类号： G01N33/6803 , C07K1/12
摘要： The present invention relates to an apparatus for collecting a peptide fragment, wherein a C-terminal peptide fragment is collected from a peptide fragment mixture resulting from specific cleavage of the peptide bond between a lysine residue and the C-terminal amino acid residue adjacent thereto. The apparatus has an immobilizing means, a cleaving means, a recovering means and a control means. According to the present invention, since the control means sequentially executes the immobilizing means, cleaving means and recovering means, no skillful work is required in peptide fragment collection, making it possible to collect the carboxyl-terminal peptide fragment with simple operation and high reproducibility.
摘要翻译：本发明涉及一种用于收集肽片段的装置，其中从由赖氨酸残基和与其相邻的C末端氨基酸残基之间的肽键的特异性切割得到的肽片段混合物收集C末端肽片段。该装置具有固定装置，切割装置，恢复装置和控制装置。根据本发明，由于控制装置依次执行固定装置，切割装置和恢复装置，因此在肽片段收集中不需要熟练的工作，使得可以以简单的操作和高再现性收集羧基末端肽片段。

8. 发明授权

US06818436B2 Micro array chip 有权
标题翻译：微阵列芯片
公开(公告)号：US06818436B2
公开(公告)日：2004-11-16
申请号：US10235971
申请日：2002-09-05
申请人： Rintaro Yamamoto , Shin Nakamura , Tsutomu Nishine , Toshihiko Yoshida
发明人： Rintaro Yamamoto , Shin Nakamura , Tsutomu Nishine , Toshihiko Yoshida
IPC分类号： C12M300
CPC分类号： H01L23/3677 , H01L2924/0002 , H01L2924/00
摘要： A micro array chip is formed by high-speed in jection molding. A chip body has a thickness of 1 mm and each well has a capacity of 1.2 &mgr;L with the bottom having a wall thickness of 250 &mgr;m. An opening of each well is surrounded by an annular protrusion part, which is raised from a surface of the chip body by a height of 200 &mgr;m. The openings of wells may be closed with a sheet of sealant made of aluminum or a resin. An overall shape of the micro array chip is rectangular and is a flat plate with a level bottom surface. Therefore, the micro array chip can be used with a heat block in flat plate form, which is independent of chip specifications such as a number of wells and their shape.
摘要翻译：微阵列芯片通过高速成型而形成。芯片的厚度为1mm，每个孔的容积为1.2μm，底部的壁厚为250μm。每个孔的开口由环形突出部分围绕，该突出部分从芯片体的表面升高200μm的高度。孔的开口可以用由铝或树脂制成的密封剂片封闭。微阵列芯片的整体形状是矩形的，并且是具有水平底面的平板。因此，微阵列芯片可以与平板形式的热块一起使用，其独立于芯片规格，例如多个孔及其形状。

9. 发明申请

US20060266649A1 Electrophoretic member, electrophoretic device, electrophoretic method and sample dispensing probe 审中-公开
标题翻译：电泳部件，电泳装置，电泳方法和样品分配探针
公开(公告)号：US20060266649A1
公开(公告)日：2006-11-30
申请号：US10571893
申请日：2004-09-21
申请人： Shin Nakamura , Rintaro Yamamoto , Toru Kaji
发明人： Shin Nakamura , Rintaro Yamamoto , Toru Kaji
IPC分类号： C07K1/26 , G01N27/00
CPC分类号： G01N27/44743
摘要： An electrophoretic member is structured such that a plate member as a base plate is provided with a separating flow path in a shape of a groove, and another plate member is provided with through-holes as small reservoirs at positions corresponding to both ends of the flow path. Both plate members are bonded together, so that the flow path is disposed inside thereof and both ends of the flow path communicate with the reservoirs to constitute an integrated base plate. Larger reservoirs having a size greater than that of the small reservoirs are connected at the positions of the small reservoirs on the plate member. Thus, a sample injection quantity can be minimized.
摘要翻译：电泳构件被构造成使得作为基板的板构件设置有凹槽形状的分离流动路径，并且另一个板构件在对应于流动两端的位置处设置有作为小储存器的通孔路径。两个板构件结合在一起，使得流路设置在其内部，并且流路的两端与储存器连通以构成一体的基板。尺寸大于小蓄水池的大型蓄水池连接在板件上的小蓄水池的位置。因此，可以使样本注入量最小化。

10. 发明授权

US07033475B2 Electrophoretic apparatus 失效
标题翻译：电泳仪
公开(公告)号：US07033475B2
公开(公告)日：2006-04-25
申请号：US09982964
申请日：2001-10-22
申请人： Shin Nakamura , Toru Kaji , Rintaro Yamamoto
发明人： Shin Nakamura , Toru Kaji , Rintaro Yamamoto
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44721 , G01N27/44743 , G01N27/44782 , G01N27/44791
摘要： An electrophoretic apparatus which can analyze multiple tests specimens and being comprised of an electrophoretic member having a relatively simple configuration of passages, such that one or a plurality of passages are formed inside a plate-shaped member therein in which holes are formed connecting to the passages at positions corresponding to the ends of each passage, a voltage application part for applying a voltage across the passages, a detector part for detecting a specimen within the passage and an electrophoretic-member holding part for holding a plurality of the electrophoretic members so as to provide for simultaneous electrophoretic operations.
摘要翻译：一种电泳装置，其可以分析多个测试样本并且由具有相对简单的通道构造的电泳构件组成，使得一个或多个通道形成在其内形成有孔的连接到通道的板状构件中在每个通道的端部对应的位置，用于在通道上施加电压的电压施加部分，用于检测通道内的样本的检测器部分和用于保持多个电泳部件的电泳部件保持部分，以便提供同步电泳操作。

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