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1. 发明授权

US5431882A Apparatus for collecting peptide fragment 失效
标题翻译：用于收集肽片段的装置
公开(公告)号：US5431882A
公开(公告)日：1995-07-11
申请号：US154515
申请日：1993-11-19
申请人： Kiyoshi Nokihara , Rintaro Yamamoto , Yoshiyuki Togawa , Mitsuhiro Hashimoto , Naoki Morita
发明人： Kiyoshi Nokihara , Rintaro Yamamoto , Yoshiyuki Togawa , Mitsuhiro Hashimoto , Naoki Morita
IPC分类号： C07K1/14 , C07K1/12 , G01N33/68
CPC分类号： G01N33/6803 , C07K1/12
摘要： The present invention relates to an apparatus for collecting a peptide fragment, wherein a C-terminal peptide fragment is collected from a peptide fragment mixture resulting from specific cleavage of the peptide bond between a lysine residue and the C-terminal amino acid residue adjacent thereto. The apparatus has an immobilizing means, a cleaving means, a recovering means and a control means. According to the present invention, since the control means sequentially executes the immobilizing means, cleaving means and recovering means, no skillful work is required in peptide fragment collection, making it possible to collect the carboxyl-terminal peptide fragment with simple operation and high reproducibility.
摘要翻译：本发明涉及一种用于收集肽片段的装置，其中从由赖氨酸残基和与其相邻的C末端氨基酸残基之间的肽键的特异性切割得到的肽片段混合物收集C末端肽片段。该装置具有固定装置，切割装置，恢复装置和控制装置。根据本发明，由于控制装置依次执行固定装置，切割装置和恢复装置，因此在肽片段收集中不需要熟练的工作，使得可以以简单的操作和高再现性收集羧基末端肽片段。

2. 发明授权

US5348883A Selecting device for cells and the like 失效
标题翻译：选择单元格的装置等
公开(公告)号：US5348883A
公开(公告)日：1994-09-20
申请号：US968530
申请日：1992-10-29
申请人： Yoshiyuki Togawa
发明人： Yoshiyuki Togawa
IPC分类号： C12M1/40 , B01L3/00 , B01L3/02 , C12M1/34 , C12N11/10 , G01N1/28 , G02B21/32 , C12M1/26
CPC分类号： B01L3/508 , C12M47/04 , B01J2219/00468 , B01L2200/0647 , B01L2300/0809 , B01L3/0241 , G01N2001/2833 , G02B21/16
摘要： Cells and the like, such as granular cells, granular microorganisms and gel beads which embrace cells or microorganisms, are arranged on a support member in a single layer. A detector detects each optical property of the cells and the like, and detectors detect each position of the cells and the like with respect to the structure. Data obtained by the detectors are stored in a memory device. According to the stored data, a controll device moves a pickup member relatively in the three dimensional directions with respect to the support member. The pickup member picks up any of the cells and the like which has a specific optical property via an adhesive. Each of the specific cells and the like which adheres to the pickup member is releasable from the pickup member.
摘要翻译：诸如颗粒细胞，颗粒微生物和包围细胞或微生物的凝胶珠等细胞等被布置在单层中的支撑构件上。检测器检测单元等的每个光学特性，并且检测器相对于该结构检测单元等的每个位置。由检测器获得的数据存储在存储器件中。根据所存储的数据，控制装置相对于相对于支撑构件的三维方向移动拾取构件。拾取构件通过粘合剂拾取具有特定光学性质的任何电池等。附着于拾取元件的每个特定电池等可从拾取元件释放。

3. 发明授权

US5587062A Sample collecting apparatus by gel electrophoresis 失效
标题翻译：凝胶电泳样品收集装置
公开(公告)号：US5587062A
公开(公告)日：1996-12-24
申请号：US592872
申请日：1996-01-24
申请人： Yoshiyuki Togawa , Makoto Miura
发明人： Yoshiyuki Togawa , Makoto Miura
IPC分类号： G01N1/28 , G01N27/447 , G01N27/26
CPC分类号： G01N27/44739 , G01N2001/288
摘要： An apparatus for collecting samples by gel electrophoresis has a base table, a cutting tool or an extractor disposed above the base table, a moving mechanism, a pattern detector and a control device. The base table is for placing thereon a gel in the form of a slab with one of its sandwiching support plates removed therefrom in such a way that the gel is exposed in the upward direction. The cutter is for cutting out a specified portion of the gel, and the extractor is for extracting a specified migration band in such a specified portion of the gel by electrophoresis. The moving mechanism is for moving the cutter, or the extractor, three-dimensionally. The pattern detector is for optically detecting migration patterns in the gel on the base table so as to enable the user to determine which part of the gel or which migration band thereon is of interest. The control device controls the motion of the moving mechanism so as to have a portion of the gel at a specified migration band to be automatically or manually cut out or to have its migration band extracted by electrophoresis.
摘要翻译：通过凝胶电泳收集样品的装置具有设置在基台上方的基台，切割工具或提取器，移动机构，图案检测器和控制装置。底座用于在其上放置其中夹着支撑板之一的板坯形式的凝胶，使得凝胶沿向上的方向暴露。切割机用于切割凝胶的指定部分，并且提取器用于通过电泳在凝胶的指定部分中提取特定的迁移带。移动机构用于三维地移动切割器或提取器。图案检测器用于光学检测基座上的凝胶中的迁移模式，以使用户能够确定凝胶的哪一部分或其上的哪个迁移带是感兴趣的。控制装置控制移动机构的运动，使得具有指定迁移带的凝胶的一部分被自动或手动切除，或者通过电泳提取其迁移带。

4. 发明授权

US5325289A Operating system for uniplanar translation of micromanipulator instruments 失效
标题翻译：微操纵器仪器的单面平移操作系统
公开(公告)号：US5325289A
公开(公告)日：1994-06-28
申请号：US083937
申请日：1993-06-28
申请人： Yoshiyuki Togawa
发明人： Yoshiyuki Togawa
IPC分类号： B25J7/00 , B25J9/18 , B25J13/06 , G02B21/32 , G05B19/409 , G06F15/46 , G05B19/18
CPC分类号： G05B19/409 , G05B2219/35438 , G05B2219/35463 , G05B2219/35466 , G05B2219/36119 , G05B2219/39452 , G05B2219/45182
摘要： In micromanipulator apparatus having fine instrument mechanism translation drivers movable along three axes within a defined space, an operating system directing uniplanar movement of the translation drivers. The system is realized in operating apparatus including a mouse as an operational data input device which, via an analogue movement thereof generating drive data, directs a correspondingly mapped uniplanar movement of the fine instrument translation drivers concerning two of the three axes, in an actual selection from a repeating sequence of dual combinations via a click button of the mouse. A controller, accordingly responsive to the drive and axes selection data input from the mouse, commands, via output to the translation drivers, movement in a plane through the two axes actually in selection. Thus operational movement and data input via the mouse effect constantly uniplanar translation of the fine instrument mechanisms, the plane of translation being continuously switchable among three planes of movement. Stable operative control of complicated micromanipulator movements, especially in micrometer increments, is furthermore enabled to be single-handed, such that two micromanipulating fine instrument mechanisms can be operated by an operator using both hands to handle the pair of corresponding mouse devices.
摘要翻译：在具有可在限定空间内沿三个轴移动的精细仪器机构平移驱动器的显微操纵器装置中，指示翻译驱动器的单面运动的操作系统。该系统在包括作为操作数据输入装置的鼠标的操作装置中实现，该操作数据输入装置经由其产生驱动数据的模拟运动，在实际选择中引导与三个轴中的两个相对应的精细仪器转换驱动器的相应映射的单平面运动从双重组合的重复序列通过鼠标的点击按钮。控制器响应于从鼠标输入的驱动和轴选择数据，经由输出到翻译驱动器，通过实际选择的两个轴在平面中的运动。因此，通过鼠标的操作运动和数据输入不断地对精细仪器机构进行单面平移，平移平面可在三个运动平面之间连续切换。复杂的微操纵器运动的稳定操作控制特别是以微米为单位进一步使得能够单手操作，使得操作者可以使用两只手操作两个微操纵的精细仪器机构来处理该对相应的鼠标装置。

5. 发明授权

US4804450A Apparatus for fusing cells 失效
标题翻译：用于融合细胞的装置
公开(公告)号：US4804450A
公开(公告)日：1989-02-14
申请号：US940119
申请日：1986-12-10
申请人： Takanori Mochizuki , Kenzo Toda , Mamoru Koga , Yoshiyuki Togawa , Shinitiro Takayama , Katsuyuki Imai
发明人： Takanori Mochizuki , Kenzo Toda , Mamoru Koga , Yoshiyuki Togawa , Shinitiro Takayama , Katsuyuki Imai
IPC分类号： C12N1/06 , C12M3/00 , C12N13/00 , C12N15/00
CPC分类号： C12N13/00 , C12M35/02
摘要： Apparatus for fusing cells, wherein the fusion chamber is provided with a pair of spaced electrodes whose opposite surfaces have such a vertical dimension as to prevent any nonuniform electric field from adversely affecting fusion of cells. The electrodes may comprise a pair of parallel plate electrodes or cocentrically arranged hollow cylindrical electrodes the outer one of which has an inner circumferential surface of a first radius while the inner electrode has an outer circumferential surface of a second radius smaller than the first radius, the difference between the first and second radii being sufficiently small as compared with the whole length of the radii.
摘要翻译：用于熔合电池的装置，其中熔融室设置有一对间隔开的电极，其相对表面具有这样的垂直尺寸，以防止任何不均匀的电场不利地影响电池的熔合。电极可以包括一对平行板电极或中心布置的中空圆柱形电极，其外部一个具有第一半径的内圆周表面，而内部电极具有比第一半径小的第二半径的外圆周表面，与半径的整个长度相比，第一和第二半径之间的差异足够小。

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